14.4.8

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보다 증폭잘된다는 TAKARA LA Taq 사용

1. srtA gene cluster for pSB1C3 cloning PCR with LA Taq

<조성>

LA Taq 0.5

Rxn buffer 5

MgCl2 5

dNTP 8

F,R Primer 4

Template 2

DW 21.5

up to 50

66,68,70 gradient, 기록안해놔서 모름..

실패

2. pEKEx2+srtA gene clutster gibson assembly cloning 할 srtA gene cluter와 pEKEx2 PCR primer 주문