Gene replacement

From CSBLwiki

Revision as of 17:34, 17 September 2010 by Igchoi (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Jump to: navigation, search

Contents

1 E. coli Gene Replacement
- 1.1 pKO3 & pKOV system
- 1.2 pKNOCK system

E. coli Gene Replacement

pKO3 & pKOV system

sacB
pSC101-ts (30C, 43C), cat
natural recombination
knockout - looking for integrates (30C->43C)
marker free - sacB (50:50)

pKNOCK system

R6k-gamma (pir+ dependent)
RP4 (oriT)
natural recombination

Error fetching PMID 10337469:

Error fetching PMID 10337469: [pKNOCK]

Retrieved from "http://compbio.korea.ac.kr/wiki/index.php/Gene_replacement"

Personal tools

Site

Choi lab

Resources

Toolbox