Genome assembly

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Coverage Graph

Using Solexa reads with Mosaik Aligner.

mosaik_aligner_result1

8번의 경우 cov가 다른 scf의 4~5배.

3번은 align 안됨. (paired-end 모드 때문?, 로슈의 gsMapper로 454 reads를 align 했을 때도 매핑이 안되었음.)

Annotation

annotation_E_limosum

tRNA_E_limosum

rRNA E_limosum

scaffolds

1. abyss : solexa
2. newbler : SE reads + PE reads + abyss fake reads (SE_PE_abyss)
3. gapRes (my_run1.fasta)
4. manually check (manual_align3.fasta)
5. minimus2 : contigs + abyss contig (after_minimus.fasta)
6. manually arrange the orientation of minimus2 with nucmer(--maxmatch [1]
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