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Line 16:		Line 16:
	**5s : all same		**5s : all same
	**16s,23s : all different		**16s,23s : all different
		+	=One plasmid=
		+	*length: 5.8 kb
		+	*Has origin?

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Revision as of 07:11, 6 February 2011

Verrucosispora sp.

Contents 1 Log 2 Read Libraries 3 Primers for gap filling 4 rRNA operon 5 One plasmid

Log

1. Illuminia PE -> ABySS -> Fake reads
2. Fake read + 454(2007.06,07) -> Newbler
3. Fake read + 454(2007.06,07,2010.09) -> Newbler

Read Libraries

• Illumina PE - 2007 8,046,794 X 2
• 454 - 2007.06 SE 249,640, 264,032
• 454 - 2007.07 SE 183,870, 187,022
• 454 - 2010.09 PE 235,916
• fake reads SE 15,379

Primers for gap filling

Primer_Verrucosispora_gap

rRNA operon

• copy number: 3
  • 5s : all same
  • 16s,23s : all different

One plasmid

• length: 5.8 kb
• Has origin?