Primer Verrucosispora gap
From CSBLwiki
(Difference between revisions)
(→List of primer) |
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*By Using Primer3 | *By Using Primer3 | ||
*"Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer). | *"Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer). | ||
| - | + | ===primer part 1 - scaffold00001=== | |
| - | > | + | >scaffold00001_1_2_1105_F |
CTCAGGTCAGGCAGGCGTGATGATCTCTTC | CTCAGGTCAGGCAGGCGTGATGATCTCTTC | ||
| - | > | + | >scaffold00001_1_2_529_R |
TACTAACCTCGGGTCGGCGTGAGAGGAGATTG | TACTAACCTCGGGTCGGCGTGAGAGGAGATTG | ||
| - | > | + | >scaffold00001_2_3_751_F |
CAGAACTGGAGAACGTCCATGCTCGAGAAC | CAGAACTGGAGAACGTCCATGCTCGAGAAC | ||
| - | > | + | >scaffold00001_2_3_1360_R |
TCGATCACCAGCTCACGGAAGTCCTCGTTG | TCGATCACCAGCTCACGGAAGTCCTCGTTG | ||
| - | > | + | >scaffold00001_3_4_586_F |
GTCAGTGCCGATCAGGATGTTGTCCAC | GTCAGTGCCGATCAGGATGTTGTCCAC | ||
| - | > | + | >scaffold00001_3_4_183_R |
ATCAACTGCCAGAACTCGTCGGTCCTCATG | ATCAACTGCCAGAACTCGTCGGTCCTCATG | ||
| - | > | + | >scaffold00001_4_5_568_F |
TACGTCGAGGAACTGGGCGACTTCAAGACC | TACGTCGAGGAACTGGGCGACTTCAAGACC | ||
| - | > | + | >scaffold00001_4_5_838_R |
| - | + | CATGTCGTCGATCTGCACGTTGACCAG | |
| - | > | + | >scaffold00001_5_6_1090_F |
GTTGAAGAAGCTGACCAGTCCGACGAGTAC | GTTGAAGAAGCTGACCAGTCCGACGAGTAC | ||
| - | > | + | >scaffold00001_5_6_374_R |
CGGGTCTTCTTCGAGATGGACATCTACTG | CGGGTCTTCTTCGAGATGGACATCTACTG | ||
| - | > | + | >scaffold00001_6_7_939_F |
ATCAGCGGTTACGTCTGGTGGTGAC | ATCAGCGGTTACGTCTGGTGGTGAC | ||
| - | > | + | >scaffold00001_6_7_870_R |
CGATCTCCACGTTTGACAGGATGCTCTG | CGATCTCCACGTTTGACAGGATGCTCTG | ||
| - | > | + | >scaffold00001_7_8_818_F |
| - | + | GAACTGGAGTTGATGGAGCAGCGCGAAAC | |
| - | > | + | >scaffold00001_7_8_545_R |
| - | + | ATCAGTTTGATCGGCGAGACGTGCGAGACC | |
| - | > | + | >scaffold00001_8_9_346_F |
| - | + | CTCGTGCTGATCGTCGAGTACGCCATCTTC | |
| - | > | + | >scaffold00001_8_9_358_R |
ATGGACATGGTCCACCTGGTCATCG | ATGGACATGGTCCACCTGGTCATCG | ||
| - | > | + | >scaffold00001_9_10_1135_F |
AGACCGGATTGAGTGCTTCCTCTGCTGGTG | AGACCGGATTGAGTGCTTCCTCTGCTGGTG | ||
| - | > | + | >scaffold00001_9_10_1408_R |
CGACCAATTTCCGACGAGGTGACATTC | CGACCAATTTCCGACGAGGTGACATTC | ||
| - | > | + | >scaffold00001_10_11_533_F |
TTCGCCACCACCGGTCTAGGATGAGAGATC | TTCGCCACCACCGGTCTAGGATGAGAGATC | ||
| - | > | + | >scaffold00001_10_11_191_R |
ATTCCTCGCGCAGTTGACCCAGTTC | ATTCCTCGCGCAGTTGACCCAGTTC | ||
| - | > | + | >scaffold00001_11_12_354_F |
AAGGTCAGGATGGCGGTGAACACCTTGGTC | AAGGTCAGGATGGCGGTGAACACCTTGGTC | ||
| - | > | + | >scaffold00001_11_12_345_R |
| - | + | ATGTTGTCGCTGACCTTCGCGATACGGGAG | |
| - | > | + | >scaffold00001_12_13_1313_F |
TTTCAGGCTTTGAGGATCGAGGTAAACAGG | TTTCAGGCTTTGAGGATCGAGGTAAACAGG | ||
| - | > | + | >scaffold00001_12_13_315_R |
TCGCATCTTCTCAGCCAGTTCCGAG | TCGCATCTTCTCAGCCAGTTCCGAG | ||
| - | > | + | >scaffold00001_13_14_961_F |
GTCATCGGACAGCCGATTCTTCGACGACTG | GTCATCGGACAGCCGATTCTTCGACGACTG | ||
| - | > | + | >scaffold00001_13_14_1221_R |
AGTCGTCGAAGCGCAGAAGGTACATGTAGG | AGTCGTCGAAGCGCAGAAGGTACATGTAGG | ||
| - | > | + | >scaffold00001_14_15_1407_F |
CTTGTTTCGATAGCATTACCGCCCAGTAAC | CTTGTTTCGATAGCATTACCGCCCAGTAAC | ||
| - | > | + | >scaffold00001_14_15_533_R |
GTACTCGTCGAAGGTGGACACGTTCACTC | GTACTCGTCGAAGGTGGACACGTTCACTC | ||
| - | > | + | >scaffold00001_15_16_126613_F |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_15_16_569_R |
CTTCTACCACGACACACACACCGATCC | CTTCTACCACGACACACACACCGATCC | ||
| - | > | + | >scaffold00001_16_17_1785_F |
CTCGAAGACGTCAACAACCACACCAATCTG | CTCGAAGACGTCAACAACCACACCAATCTG | ||
| - | > | + | >scaffold00001_16_17_478_R |
CTCGTAACTCTCCATCGGTTCTCCAGTAAC | CTCGTAACTCTCCATCGGTTCTCCAGTAAC | ||
| - | > | + | >scaffold00001_17_18_225_F |
GATCGTGACGAACGTGAAGGTCCAC | GATCGTGACGAACGTGAAGGTCCAC | ||
| - | > | + | >scaffold00001_17_18_408_R |
CAGATCCGGTACAGCTCCTTGTATTCCAG | CAGATCCGGTACAGCTCCTTGTATTCCAG | ||
| - | > | + | >scaffold00001_18_19_320_F |
CAGATAGATGGTGAAGTCAGGACGACGG | CAGATAGATGGTGAAGTCAGGACGACGG | ||
| - | > | + | >scaffold00001_18_19_177_R |
ATGAACTTTCCACCGATGCCGGTCGTCTTC | ATGAACTTTCCACCGATGCCGGTCGTCTTC | ||
| - | > | + | >scaffold00001_19_20_313_F |
| - | + | GTGTCGTTGTAGGCGATGAGGAGTTGGTG | |
| - | > | + | >scaffold00001_19_20_645_R |
| - | + | CTGTCTACAACATCCCCGTCGCACTCCAAC | |
| - | > | + | >scaffold00001_20_21_312_F |
TGTCGTTGTAGGCGACGAGGAGCTGATCAC | TGTCGTTGTAGGCGACGAGGAGCTGATCAC | ||
| - | > | + | >scaffold00001_20_21_428_R |
| - | + | TCGGACATGTCTTCGACCTGAGTGC | |
| - | > | + | >scaffold00001_21_22_243_F |
AAGGACGGACGTGGACATCGATCAGCAATC | AAGGACGGACGTGGACATCGATCAGCAATC | ||
| - | > | + | >scaffold00001_21_22_1379_R |
AGGACTCCTGTACGTCGATCACGACGAGTG | AGGACTCCTGTACGTCGATCACGACGAGTG | ||
| - | > | + | >scaffold00001_22_23_238_F |
GTTTCACTCATCGGTGTACCCTCCCGATC | GTTTCACTCATCGGTGTACCCTCCCGATC | ||
| - | > | + | >scaffold00001_22_23_244_R |
| - | + | GAAGTTCGTCATCGAGAACGTCTCCGTGC | |
| - | > | + | >scaffold00001_23_24_410_F |
ACTGACCACGGTGCAGCTCTACTTCAC | ACTGACCACGGTGCAGCTCTACTTCAC | ||
| - | > | + | >scaffold00001_23_24_1184_R |
AGCGTGTAGTCGGCGGTATACACCTTC | AGCGTGTAGTCGGCGGTATACACCTTC | ||
| - | > | + | >scaffold00001_24_25_444_F |
ATTCGACGGGCAGGACGTCAACAAG | ATTCGACGGGCAGGACGTCAACAAG | ||
| - | > | + | >scaffold00001_24_25_458_R |
| - | + | TAGGTGTGCTCCAGGTAGGTGAGGAAC | |
| - | > | + | >scaffold00001_25_26_203_F |
| - | + | GTCGAGGGGATCAACACAAGTGGAATCAAG | |
| - | > | + | >scaffold00001_25_26_184_R |
GTACCGTCGACAAGCAACTCCTGATCGTC | GTACCGTCGACAAGCAACTCCTGATCGTC | ||
| - | > | + | >scaffold00001_26_27_369_F |
ATGAGCGAGCAGGTCTTCCTCCTAC | ATGAGCGAGCAGGTCTTCCTCCTAC | ||
| - | > | + | >scaffold00001_26_27_706_R |
GAACACGCCGTAGTTGTTCAGCGTGAAG | GAACACGCCGTAGTTGTTCAGCGTGAAG | ||
| - | > | + | >scaffold00001_27_28_280_F |
TGACGTGAGGGGACTACGGATGTAC | TGACGTGAGGGGACTACGGATGTAC | ||
| - | > | + | >scaffold00001_27_28_260_R |
ACTTCGACAGGTAGGCCTTGCAGAG | ACTTCGACAGGTAGGCCTTGCAGAG | ||
| - | > | + | >scaffold00001_28_29_209_F |
TCTACGGTGGGTTGCACTGGTTTAGAGAAG | TCTACGGTGGGTTGCACTGGTTTAGAGAAG | ||
| - | > | + | >scaffold00001_28_29_357_R |
TACAGCTGAAGGATCTTCTCGTCGAGGACC | TACAGCTGAAGGATCTTCTCGTCGAGGACC | ||
| - | > | + | >scaffold00001_29_30_1643_F |
CCGATCCAGGCGAAGATCAGATTGGTC | CCGATCCAGGCGAAGATCAGATTGGTC | ||
| - | > | + | >scaffold00001_29_30_767_R |
ATCGACATAGCGCTCCAACAGATCGGTGAC | ATCGACATAGCGCTCCAACAGATCGGTGAC | ||
| - | > | + | >scaffold00001_30_31_237_F |
CACCACACGTTGTACGTCTTCGTGCTCTAC | CACCACACGTTGTACGTCTTCGTGCTCTAC | ||
| - | > | + | >scaffold00001_30_31_286_R |
| - | + | GACATCCCCCAAGTCTTTCCTCTCATGCTC | |
| - | > | + | >scaffold00001_31_32_566_F |
AAACGAGCCGTACAACGGGCAACTG | AAACGAGCCGTACAACGGGCAACTG | ||
| - | > | + | >scaffold00001_31_32_169_R |
| - | + | ATCTTCCTGACCTCGTCGGTGTGGTACTTC | |
| - | > | + | >scaffold00001_32_33_266_F |
| - | + | AACATTGACAGGGTGGCACGCAGTTCG | |
| - | > | + | >scaffold00001_32_33_169_R |
| - | + | TTGTCAATGTAGCGTTGACGGGTGCCTGAC | |
| - | > | + | >scaffold00001_33_34_795_F |
GTCGACGACGAGAATCCGGTTCATG | GTCGACGACGAGAATCCGGTTCATG | ||
| - | > | + | >scaffold00001_33_34_365_R |
GTTCCCTGCTGCTGAACTGGTTCTTCAC | GTTCCCTGCTGCTGAACTGGTTCTTCAC | ||
| - | > | + | >scaffold00001_34_35_1071_F |
| - | + | CAGTACGTCATCTTCGACCGGACCATTTG | |
| - | > | + | >scaffold00001_34_35_2096_R |
AGTCGTCGTCGTCGAACATCGTGAC | AGTCGTCGTCGTCGAACATCGTGAC | ||
| - | > | + | >scaffold00001_35_36_685_F |
GTCGTTCAGGTTCAGGTAGAACTCGGTGAC | GTCGTTCAGGTTCAGGTAGAACTCGGTGAC | ||
| - | > | + | >scaffold00001_35_36_0_R |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_36_37_1073_F |
| - | + | GATCGGTTTGCCGCGGTCGTTGTATTCGAC | |
| - | > | + | >scaffold00001_36_37_740_R |
ATACGGCTTCGTCGAGAACCTGACC | ATACGGCTTCGTCGAGAACCTGACC | ||
| - | > | + | >scaffold00001_37_38_498_F |
TTCGACGAACTGTGGCAACGCTTCTCC | TTCGACGAACTGTGGCAACGCTTCTCC | ||
| - | > | + | >scaffold00001_37_38_562_R |
AGGACGTCATGCACGGTCAACACAC | AGGACGTCATGCACGGTCAACACAC | ||
| - | > | + | >scaffold00001_38_39_612_F |
| - | + | AACATGTCGTTGAGCGCGGCCAGTTTG | |
| - | > | + | >scaffold00001_38_39_672_R |
CTCTTCGACGAGATCATCCAGGTGCTG | CTCTTCGACGAGATCATCCAGGTGCTG | ||
| - | > | + | >scaffold00001_39_40_196_F |
| - | + | CACAATTTCATCGATCAACACAAACGCGAG | |
| - | > | + | >scaffold00001_39_40_425_R |
ACCTGCAACTGACTCGGCTGGCTATAGCTG | ACCTGCAACTGACTCGGCTGGCTATAGCTG | ||
| - | > | + | >scaffold00001_40_41_522_F |
GACTACAAGCCGGAGAACGTGCTCATCTC | GACTACAAGCCGGAGAACGTGCTCATCTC | ||
| - | > | + | >scaffold00001_40_41_660_R |
GTGCAGAAGGGATAGAGGGTGACGTAC | GTGCAGAAGGGATAGAGGGTGACGTAC | ||
| - | > | + | >scaffold00001_41_42_205_F |
ACCGAAGCAGTCGAAGACGGTACAC | ACCGAAGCAGTCGAAGACGGTACAC | ||
| - | > | + | >scaffold00001_41_42_339_R |
| - | + | GTCTTCTTCTGGCCCCGCAACGAGATCATG | |
| - | > | + | >scaffold00001_42_43_389_F |
TAAAATCTGTGATCTGGGCTAACCTTGGGG | TAAAATCTGTGATCTGGGCTAACCTTGGGG | ||
| - | > | + | >scaffold00001_42_43_223_R |
GTGATCGGTGTGTTCGTGGCTGACCAGTAC | GTGATCGGTGTGTTCGTGGCTGACCAGTAC | ||
| - | > | + | >scaffold00001_43_44_440_F |
CTCGGAGGAAAACGGTGAACAGTCAGCAAC | CTCGGAGGAAAACGGTGAACAGTCAGCAAC | ||
| - | > | + | >scaffold00001_43_44_563_R |
TGAACGCCTCCACCCAGCATTTGATCACAG | TGAACGCCTCCACCCAGCATTTGATCACAG | ||
| - | > | + | >scaffold00001_44_45_93801_F |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_44_45_485_R |
GTGTTGCGATCGGATTCGTAGCTGAC | GTGTTGCGATCGGATTCGTAGCTGAC | ||
| - | > | + | >scaffold00001_45_46_401_F |
GGATCAGCTCCGGATAGTTGAAGTCGTC | GGATCAGCTCCGGATAGTTGAAGTCGTC | ||
| - | > | + | >scaffold00001_45_46_423_R |
ACAACGAGGACTTCGACAACGGCTTCAAGG | ACAACGAGGACTTCGACAACGGCTTCAAGG | ||
| - | > | + | >scaffold00001_46_47_370_F |
| - | + | GTAAACTCCTGATCTCAGCCGCTCTCGTG | |
| - | > | + | >scaffold00001_46_47_845_R |
CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG | CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG | ||
| - | > | + | >scaffold00001_47_48_1970_F |
GACGACTACCTGCGTAAACCCTTCGACCTG | GACGACTACCTGCGTAAACCCTTCGACCTG | ||
| - | > | + | >scaffold00001_47_48_1044_R |
| - | + | CACCAATCTGCTTGTGGGTCTGGTGCTGAC | |
| - | > | + | >scaffold00001_48_49_1639_F |
GATGTTCATCGACCTGGAGAACGTGC | GATGTTCATCGACCTGGAGAACGTGC | ||
| - | > | + | >scaffold00001_48_49_244_R |
| - | + | CATCTCTCCCGTCAGCTCATCGTTCGAAG | |
| - | > | + | >scaffold00001_49_50_1297_F |
CAGGGTTTCCTGAGCCACCTTGAAC | CAGGGTTTCCTGAGCCACCTTGAAC | ||
| - | > | + | >scaffold00001_49_50_822_R |
CTGTTCTCCCAGGACCCGATCTTCAACTC | CTGTTCTCCCAGGACCCGATCTTCAACTC | ||
| - | > | + | >scaffold00001_50_51_227_F |
CGTGCCATGTTCTGAACGAGAACGATC | CGTGCCATGTTCTGAACGAGAACGATC | ||
| - | > | + | >scaffold00001_50_51_860_R |
GTGACCAGCGAGTGAATCTTGTCGCGTTG | GTGACCAGCGAGTGAATCTTGTCGCGTTG | ||
| - | > | + | >scaffold00001_51_52_1410_F |
GATGTCCACGTCGTACGCCATGCTGTTGTC | GATGTCCACGTCGTACGCCATGCTGTTGTC | ||
| - | > | + | >scaffold00001_51_52_509_R |
CCTGATTCACCTGATTCGTCCACCAGTTC | CCTGATTCACCTGATTCGTCCACCAGTTC | ||
| - | > | + | >scaffold00001_52_53_361_F |
| - | + | GTCATCCACACATGGCATTCTGTGACC | |
| - | > | + | >scaffold00001_52_53_353_R |
AGGATGGCGTAATCCGCGATGATGG | AGGATGGCGTAATCCGCGATGATGG | ||
| - | > | + | >scaffold00001_53_54_414_F |
TTCTGGAAGACCATCTGCAGACCGATCTCG | TTCTGGAAGACCATCTGCAGACCGATCTCG | ||
| - | > | + | >scaffold00001_53_54_823_R |
TACGCGAAGGGACGTTAGAGATGGCAC | TACGCGAAGGGACGTTAGAGATGGCAC | ||
| - | > | + | >scaffold00001_54_55_1250_F |
CTGGAACCGGATGATGTTGGAGTCCTC | CTGGAACCGGATGATGTTGGAGTCCTC | ||
| - | > | + | >scaffold00001_54_55_175_R |
| - | + | TACCGAGGAACTGATCGACCTCTCC | |
| - | > | + | >scaffold00001_55_56_239_F |
CACCTGACCTCTGACAATGAACGCCGAAAG | CACCTGACCTCTGACAATGAACGCCGAAAG | ||
| - | > | + | >scaffold00001_55_56_206_R |
GATTGGCTTCTATACAAACTAAGGTGGCGC | GATTGGCTTCTATACAAACTAAGGTGGCGC | ||
| - | > | + | >scaffold00001_56_57_412_F |
| - | + | GTACTCACGATGGTCCTTCTCCTCGTTG | |
| - | > | + | >scaffold00001_56_57_184_R |
CAAACCGGTGGTCGGGCTGATCATGTATTC | CAAACCGGTGGTCGGGCTGATCATGTATTC | ||
| - | > | + | >scaffold00001_57_58_1204_F |
TCATGCTGTCCGTATCCATTTAGGACCTTC | TCATGCTGTCCGTATCCATTTAGGACCTTC | ||
| - | > | + | >scaffold00001_57_58_939_R |
TTCTACCGTGCCTACTTCGAGACCGAC | TTCTACCGTGCCTACTTCGAGACCGAC | ||
| - | > | + | >scaffold00001_58_59_788_F |
CAAAGCAAATCGGAGGGGACATGACG | CAAAGCAAATCGGAGGGGACATGACG | ||
| - | > | + | >scaffold00001_58_59_229_R |
ACACGTTCGTCCGCAACAACGGATG | ACACGTTCGTCCGCAACAACGGATG | ||
| - | > | + | >scaffold00001_59_60_1979_F |
GTGACCAGCCAGGTCTGTTCGAATCTCAC | GTGACCAGCCAGGTCTGTTCGAATCTCAC | ||
| - | > | + | >scaffold00001_59_60_625_R |
| - | + | GAGATCATGTACGCCGACTTCAGCTTCGTG | |
| - | > | + | >scaffold00001_60_61_755_F |
GTACGGTTTCTGACCAACACCGACTCGAAG | GTACGGTTTCTGACCAACACCGACTCGAAG | ||
| - | > | + | >scaffold00001_60_61_647_R |
CCAACATCACCATCACGAACCTCGG | CCAACATCACCATCACGAACCTCGG | ||
| - | > | + | >scaffold00001_61_62_285_F |
TCAGGTTGAGATCGACCATCGCGAG | TCAGGTTGAGATCGACCATCGCGAG | ||
| - | > | + | >scaffold00001_61_62_944_R |
| - | + | TTGCACCATCAGCTCAGCATCGTCTTCGAC | |
| - | > | + | >scaffold00001_62_63_898_F |
CTGATGATCAAGGTGTTGCGGCTGTC | CTGATGATCAAGGTGTTGCGGCTGTC | ||
| - | > | + | >scaffold00001_62_63_367_R |
GTTGTAGCCGCCGAAGTCGTTCATGCTGAC | GTTGTAGCCGCCGAAGTCGTTCATGCTGAC | ||
| - | > | + | >scaffold00001_63_64_711_F |
ACGTAGTTCCCGACCGTAGAGCAGTTC | ACGTAGTTCCCGACCGTAGAGCAGTTC | ||
| - | > | + | >scaffold00001_63_64_1286_R |
| - | + | CGATAGGTGAGACTCGATGCATGGTTAGTG | |
| - | > | + | >scaffold00001_64_65_324_F |
CTGGTGTGCTTTTCGGTTCGGGTAC | CTGGTGTGCTTTTCGGTTCGGGTAC | ||
| - | > | + | >scaffold00001_64_65_297_R |
AACGCCCACGTCATCCTCGAAGAAC | AACGCCCACGTCATCCTCGAAGAAC | ||
| - | > | + | >scaffold00001_65_66_1986_F |
GATCAGTCCGGCGATCGACGAGTAGAGAAC | GATCAGTCCGGCGATCGACGAGTAGAGAAC | ||
| - | > | + | >scaffold00001_65_66_1292_R |
GTTGCTGCTGGAAGTGGCATGGGAAAC | GTTGCTGCTGGAAGTGGCATGGGAAAC | ||
| - | > | + | >scaffold00001_66_67_428_F |
CTTCGTCACTGGCTGTGTCAAGGTCAAC | CTTCGTCACTGGCTGTGTCAAGGTCAAC | ||
| - | > | + | >scaffold00001_66_67_346_R |
ACTGTTCCTGCTGTACTCCTCGGTG | ACTGTTCCTGCTGTACTCCTCGGTG | ||
| - | > | + | >scaffold00001_67_68_5122_F |
| - | + | ||
| - | + | ||
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_67_68_0_R |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_68_69_5187_F |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_68_69_1373_R |
| + | CTCCTGGTGGGCTCGGTGAAAACCAACATC | ||
| + | >scaffold00001_69_70_254_F | ||
CTTCGACATGGAGGCAGTGCTTGAGAACTC | CTTCGACATGGAGGCAGTGCTTGAGAACTC | ||
| - | > | + | >scaffold00001_69_70_318_R |
AGTGAGGAGGAACAGTTCTACAATGGTGTG | AGTGAGGAGGAACAGTTCTACAATGGTGTG | ||
| - | > | + | >scaffold00001_70_71_240_F |
CATTCGGCGTGGTAAAGACCCACTGGTAC | CATTCGGCGTGGTAAAGACCCACTGGTAC | ||
| - | > | + | >scaffold00001_70_71_233_R |
ATCGATGACCCTCCAAGACAGACCTCATTG | ATCGATGACCCTCCAAGACAGACCTCATTG | ||
| - | > | + | >scaffold00001_71_72_349_F |
AACCCACGCATCCCACTCAACGAGCTGAAC | AACCCACGCATCCCACTCAACGAGCTGAAC | ||
| - | > | + | >scaffold00001_71_72_0_R |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_72_73_2964_F |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_72_73_0_R |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_73_74_2968_F |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_73_74_0_R |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_74_75_356_F |
GTGTTCACCGGCGTGATGTACAACGACTAC | GTGTTCACCGGCGTGATGTACAACGACTAC | ||
| - | > | + | >scaffold00001_74_75_0_R |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_75_76_788_F |
GTCGACGTTTCATCACGTCACTGGGTG | GTCGACGTTTCATCACGTCACTGGGTG | ||
| - | > | + | >scaffold00001_75_76_0_R |
Can't find proper primer | Can't find proper primer | ||
| - | > | + | >scaffold00001_76_77_1854_F |
GTGATCAAGGTGATCGAAGCCCTGC | GTGATCAAGGTGATCGAAGCCCTGC | ||
| - | > | + | >scaffold00001_76_77_535_R |
ATCTACAACCACCTGGGCGGCAAGGAGAAC | ATCTACAACCACCTGGGCGGCAAGGAGAAC | ||
| - | > | + | >scaffold00001_77_78_624_F |
| - | + | AATCTCATGGGGTGCCTTCGTCGATCGGTGAG | |
| - | > | + | >scaffold00001_77_78_349_R |
CTCGGCAACCGACTGTGGAACAACATC | CTCGGCAACCGACTGTGGAACAACATC | ||
| - | > | + | >scaffold00001_78_79_195_F |
| - | + | GTAGTTGCACGAGACGGCCATTACCTTG | |
| - | > | + | >scaffold00001_78_79_276_R |
GAAATGCCGTCACTCTCCGTAGATGTCC | GAAATGCCGTCACTCTCCGTAGATGTCC | ||
| - | > | + | >scaffold00001_79_80_301_F |
TACGTTTGGCTCAACACCCCACGCGAGATG | TACGTTTGGCTCAACACCCCACGCGAGATG | ||
| - | > | + | >scaffold00001_79_80_212_R |
GTCGCTGATCATGGTTCGGTAGACGATGTC | GTCGCTGATCATGGTTCGGTAGACGATGTC | ||
| - | > | + | >scaffold00001_80_81_426_F |
CACTGCCCAAACCGATCTATCCGACGTTC | CACTGCCCAAACCGATCTATCCGACGTTC | ||
| - | > | + | >scaffold00001_80_81_202_R |
GATCCAAGCTGTCTTAACGAGGAGTTGCC | GATCCAAGCTGTCTTAACGAGGAGTTGCC | ||
| - | > | + | >scaffold00001_81_82_751_F |
CAAATCCGGGCACCTCGAACTCAAG | CAAATCCGGGCACCTCGAACTCAAG | ||
| - | > | + | >scaffold00001_81_82_233_R |
AACACCACCACCCCCGAGTAGCAGACAACAAC | AACACCACCACCCCCGAGTAGCAGACAACAAC | ||
| - | > | + | >scaffold00001_82_83_253_F |
CAAGGTCATCGTCAGCGGTGGATTG | CAAGGTCATCGTCAGCGGTGGATTG | ||
| - | > | + | >scaffold00001_82_83_302_R |
AGATCCGGATACTGCGCTGGGAAAC | AGATCCGGATACTGCGCTGGGAAAC | ||
| - | > | + | >scaffold00001_83_84_243_F |
GTGGGGCAAGTGCTGGAGTTTGATTCAC | GTGGGGCAAGTGCTGGAGTTTGATTCAC | ||
| - | > | + | >scaffold00001_83_84_182_R |
GTGGACGGACCAGTCGTGATGTTCGATTC | GTGGACGGACCAGTCGTGATGTTCGATTC | ||
| - | > | + | >scaffold00001_84_85_414_F |
| - | + | GTTTACCCTCGATCCGGCAGATGAAGC | |
| - | > | + | >scaffold00001_84_85_726_R |
GACCCGTCCAACACCTTCGATCAGAGCTTC | GACCCGTCCAACACCTTCGATCAGAGCTTC | ||
| - | > | + | >scaffold00001_85_86_202_F |
| - | + | GTTTAGGAAGTGTGGACACGGCCGGAAGTC | |
| - | > | + | >scaffold00001_85_86_191_R |
CCGAAGTGTGCAAACGTTGTCTTTACGTC | CCGAAGTGTGCAAACGTTGTCTTTACGTC | ||
| - | > | + | >scaffold00001_86_87_218_F |
TCAACAACCCAGTGAACACCGAGGACGTAC | TCAACAACCCAGTGAACACCGAGGACGTAC | ||
| - | > | + | >scaffold00001_86_87_245_R |
| - | + | CATTGAGAAAGCGATGTACCGCCACGAGAC | |
| - | > | + | >scaffold00001_87_88_204_F |
GAAGTGATCACGCGATCCACGGGATTCCAC | GAAGTGATCACGCGATCCACGGGATTCCAC | ||
| - | > | + | >scaffold00001_87_88_267_R |
AGCTTCCAGACCCTGGTCAACAGTC | AGCTTCCAGACCCTGGTCAACAGTC | ||
| - | > | + | >scaffold00001_88_89_1973_F |
AGTAGCCGATGACCCCGTTGAGTTG | AGTAGCCGATGACCCCGTTGAGTTG | ||
| - | > | + | >scaffold00001_88_89_463_R |
CAGGTGTTCAACCTCGCCCAGACCTTCTAC | CAGGTGTTCAACCTCGCCCAGACCTTCTAC | ||
| - | > | + | >scaffold00001_89_90_613_F |
ATGACCTTCGTGAAACTGACCTTCTCGCTG | ATGACCTTCGTGAAACTGACCTTCTCGCTG | ||
| - | > | + | >scaffold00001_89_90_528_R |
GACAAGGACTCCGCCAAGAAGGACGAATC | GACAAGGACTCCGCCAAGAAGGACGAATC | ||
| - | > | + | |
| + | |||
| + | ===primer part 2 - scaffold00003, scaffold00004=== | ||
| + | |||
| + | >scaffold00003_1_2_276_F | ||
GACGTGTACATCTCGAACATCTCGCGG | GACGTGTACATCTCGAACATCTCGCGG | ||
| - | > | + | >scaffold00003_1_2_387_R |
| - | + | AAAACGGGCGTTCACCGCATGGTAC | |
| - | > | + | >scaffold00003_2_3_232_F |
CAAGGGAGAGTTCCGTTACGACCACAG | CAAGGGAGAGTTCCGTTACGACCACAG | ||
| - | > | + | >scaffold00003_2_3_460_R |
TTGATCAGCCGGCACAGCAACAAGGTGGTG | TTGATCAGCCGGCACAGCAACAAGGTGGTG | ||
| - | > | + | >scaffold00003_3_4_218_F |
ACAAGCTCATTGCCTACTAGATCGACTTCG | ACAAGCTCATTGCCTACTAGATCGACTTCG | ||
| - | > | + | >scaffold00003_3_4_1054_R |
| - | + | GCATCGTCCAACGGTAGCCTTCTTTC | |
| - | > | + | >scaffold00003_4_5_574_F |
ATCGACCTGGTGCACGCGAACAACTCCAAG | ATCGACCTGGTGCACGCGAACAACTCCAAG | ||
| - | > | + | >scaffold00003_4_5_289_R |
GAAGAAGGCGTTGGAGTGCCACAGTTG | GAAGAAGGCGTTGGAGTGCCACAGTTG | ||
| - | > | + | >scaffold00003_5_6_195_F |
| - | + | ATCTGTTGCCGAATTGGCCTGTACTAGATC | |
| - | > | + | >scaffold00003_5_6_263_R |
ATGGCATCCCTCAACCACTACCACC | ATGGCATCCCTCAACCACTACCACC | ||
| - | > | + | >scaffold00003_6_7_918_F |
ATGCTCAGTCCGGGAGTACAGCTCGGTAAC | ATGCTCAGTCCGGGAGTACAGCTCGGTAAC | ||
| - | > | + | >scaffold00003_6_7_245_R |
| - | + | GCATGCTGTTGTTGATGCTGTCCTTCAG | |
| - | > | + | >scaffold00003_7_8_365_F |
ATCCGACGAGTACGACATCCACCTG | ATCCGACGAGTACGACATCCACCTG | ||
| - | > | + | >scaffold00003_7_8_601_R |
CAGACCAACTACGCCACAAGCAAGG | CAGACCAACTACGCCACAAGCAAGG | ||
| - | > | + | >scaffold00003_8_9_472_F |
CTGTTCCAGACCTTCCTCACCGTGCTTGAC | CTGTTCCAGACCTTCCTCACCGTGCTTGAC | ||
| - | > | + | >scaffold00003_8_9_218_R |
CTTATGCCCTGACAGAAGGTGCAAGATCAG | CTTATGCCCTGACAGAAGGTGCAAGATCAG | ||
| - | > | + | >scaffold00003_9_10_376_F |
CATGCACAAGCAGGACCTCATCGAG | CATGCACAAGCAGGACCTCATCGAG | ||
| - | > | + | >scaffold00003_9_10_212_R |
TTTCCGTTGAGCCGCCACTCGTACTTGTAG | TTTCCGTTGAGCCGCCACTCGTACTTGTAG | ||
| - | > | + | >scaffold00003_10_11_1436_F |
TGGTCCTGCCGTACTTCCTGATCACCACTG | TGGTCCTGCCGTACTTCCTGATCACCACTG | ||
| - | > | + | >scaffold00003_10_11_232_R |
GATGGGACAGACTTCGTACACCGGTTG | GATGGGACAGACTTCGTACACCGGTTG | ||
| - | > | + | >scaffold00003_11_12_266_F |
CTGTATTGATGATCCACTCGCTGCCGCTC | CTGTATTGATGATCCACTCGCTGCCGCTC | ||
| - | > | + | >scaffold00003_11_12_1184_R |
TACAGTGATCGGCCGTTGTTGGAGTGTGTG | TACAGTGATCGGCCGTTGTTGGAGTGTGTG | ||
| - | > | + | >scaffold00003_12_13_2070_F |
CTCCAGCAGGGTTTCATCGATTCGGTAG | CTCCAGCAGGGTTTCATCGATTCGGTAG | ||
| - | > | + | >scaffold00003_12_13_324_R |
CTGGACCAGTTCACCCTCCAGATCATG | CTGGACCAGTTCACCCTCCAGATCATG | ||
| - | > | + | >scaffold00003_13_14_463_F |
| - | + | CTGGTGTCACACAACGAAAAGGACCTGAC | |
| - | > | + | >scaffold00003_13_14_304_R |
GAAGAAGTCGTCCTTCTCCTTGACCGACAG | GAAGAAGTCGTCCTTCTCCTTGACCGACAG | ||
| - | > | + | >scaffold00003_14_15_137305_F |
| - | + | Can't find proper primer | |
| - | > | + | >scaffold00003_14_15_518_R |
AATACCGCTACGGTGCTGGAACCGATGTAC | AATACCGCTACGGTGCTGGAACCGATGTAC | ||
| - | > | + | >scaffold00003_15_16_227_F |
GTTTCTGGACAAGTACGGTTTCGGGACC | GTTTCTGGACAAGTACGGTTTCGGGACC | ||
| - | > | + | >scaffold00003_15_16_170_R |
| - | + | CTTGAGGCGCTCACTGATGCACTTAAC | |
| - | > | + | >scaffold00003_16_17_319_F |
| - | + | TCACGTGATCTCCTGATAGGTAGACAGACG | |
| - | > | + | >scaffold00003_16_17_503_R |
TACCTGTGGCCCCAAAGCTACTACCTG | TACCTGTGGCCCCAAAGCTACTACCTG | ||
| - | > | + | >scaffold00003_17_18_314_F |
| - | + | TCTGAACGAGACGGAATGGGAGATCCTCAC | |
| - | > | + | >scaffold00003_17_18_222_R |
GACATCACCATCCAGGCAGAGAACATGCTG | GACATCACCATCCAGGCAGAGAACATGCTG | ||
| - | > | + | >scaffold00003_18_19_746_F |
GTTGCTTCGAATCTGGTGACGGGAG | GTTGCTTCGAATCTGGTGACGGGAG | ||
| - | > | + | >scaffold00003_18_19_471_R |
GTATACCGCAACATCGGCGAAATGAAGTC | GTATACCGCAACATCGGCGAAATGAAGTC | ||
| - | > | + | >scaffold00003_19_20_655_F |
GAAGGAACGGCAACTTTCGGTCACGGTAC | GAAGGAACGGCAACTTTCGGTCACGGTAC | ||
| - | > | + | >scaffold00003_19_20_485_R |
CAAGGAGACCGTCTACAAGATCTGGCATC | CAAGGAGACCGTCTACAAGATCTGGCATC | ||
| - | > | + | >scaffold00003_20_21_294_F |
| - | + | ATCAGCCCCGAGCAATTGACCGACCTGAAC | |
| - | > | + | >scaffold00003_20_21_200_R |
GAAGATCGGCAGCTCCATCGTCTCATC | GAAGATCGGCAGCTCCATCGTCTCATC | ||
| - | > | + | >scaffold00003_21_22_1034_F |
GAAGTAACCGGTGGTGTTTTCGGCCAG | GAAGTAACCGGTGGTGTTTTCGGCCAG | ||
| - | > | + | >scaffold00003_21_22_354_R |
ATCTCGCCGGTTACAAGCAGATCCTC | ATCTCGCCGGTTACAAGCAGATCCTC | ||
| - | > | + | >scaffold00003_22_23_1299_F |
TTCAGATGGTTAAGGACAGCTTCATGAGCG | TTCAGATGGTTAAGGACAGCTTCATGAGCG | ||
| - | > | + | >scaffold00003_22_23_368_R |
ACGTGATCGGTGGGAACTTCGACTC | ACGTGATCGGTGGGAACTTCGACTC | ||
| - | > | + | >scaffold00003_23_24_235_F |
CCCAGGGAATATAACGATGGTTCAGGTCAG | CCCAGGGAATATAACGATGGTTCAGGTCAG | ||
| - | > | + | >scaffold00003_23_24_1163_R |
| - | + | GAACTGCTGGTTCATCGAATGGTCGTTGAC | |
| - | > | + | >scaffold00003_24_25_268_F |
| - | + | CTCGAAGTCCCGGTCAAGAAGATCCTCAC | |
| - | > | + | >scaffold00003_24_25_666_R |
TATCGTTCGCGTCGATGGCCGACAACATTG | TATCGTTCGCGTCGATGGCCGACAACATTG | ||
| - | > | + | >scaffold00003_25_26_360_F |
GAAAGGCTGACCATCTACCCGGAGTAC | GAAAGGCTGACCATCTACCCGGAGTAC | ||
| - | > | + | >scaffold00003_25_26_636_R |
| - | + | TGATCCACGTGTCCGTACATCATCGTG | |
| - | > | + | >scaffold00003_26_27_1270_F |
| - | + | GAAATCAAGATCCTGAACCAGTCCCGGAAG | |
| - | > | + | >scaffold00003_26_27_244_R |
| - | + | CCCGATTCCTTGTCCTGGAACAGATAGAAG | |
| - | > | + | >scaffold00003_27_28_833_F |
GTACTCGCCGACTTTCCCGTTCTCATC | GTACTCGCCGACTTTCCCGTTCTCATC | ||
| - | > | + | >scaffold00003_27_28_474_R |
GTACACCTCGTGCTCGGGACACAAATGGTC | GTACACCTCGTGCTCGGGACACAAATGGTC | ||
| - | > | + | >scaffold00003_28_29_210_F |
GTACACGTGCCGTTCCCGTTTCTGGTGAAC | GTACACGTGCCGTTCCCGTTTCTGGTGAAC | ||
| - | > | + | >scaffold00003_28_29_173_R |
| - | + | ACGGCGTCACGAACAACACCTACTG | |
| - | > | + | >scaffold00003_29_30_703_F |
TCAGCGATTCAGAGGTCCCGATCAGAGGTC | TCAGCGATTCAGAGGTCCCGATCAGAGGTC | ||
| - | > | + | >scaffold00003_29_30_1341_R |
TACCTGCATACCGTACGTGGAGTCG | TACCTGCATACCGTACGTGGAGTCG | ||
| - | > | + | >scaffold00003_30_31_256_F |
TTCCGGGGATGAGTAGCCGACCAATTGTCC | TTCCGGGGATGAGTAGCCGACCAATTGTCC | ||
| - | > | + | >scaffold00003_30_31_1239_R |
GATCACCGGTTGGTAGACCAGATCCAG | GATCACCGGTTGGTAGACCAGATCCAG | ||
| - | > | + | >scaffold00003_31_32_351_F |
CACAAATTGAACTTGAGCTGGGATGTCAGG | CACAAATTGAACTTGAGCTGGGATGTCAGG | ||
| - | > | + | >scaffold00003_31_32_565_R |
CAGGATGACAGTGTGAGTCAGCCATGGAC | CAGGATGACAGTGTGAGTCAGCCATGGAC | ||
| - | > | + | >scaffold00003_32_33_376_F |
AGATCGACGAGCTCAACGTCTATCCG | AGATCGACGAGCTCAACGTCTATCCG | ||
| - | > | + | >scaffold00003_32_33_1069_R |
GTCATTCGACACCCACCAGCAACGGATAG | GTCATTCGACACCCACCAGCAACGGATAG | ||
| - | > | + | >scaffold00003_33_34_1969_F |
| - | + | GTCCTCAAGCATCGCAAGCGCAAGGAAAAG | |
| - | > | + | >scaffold00003_33_34_485_R |
CTCCAACTGCTCGGAGAGGAACTTGTAAC | CTCCAACTGCTCGGAGAGGAACTTGTAAC | ||
| - | > | + | >scaffold00003_34_35_491_F |
GAACGAGACCAGGAGACATAGTGGCGAGTC | GAACGAGACCAGGAGACATAGTGGCGAGTC | ||
| - | > | + | >scaffold00003_34_35_222_R |
ATCTCCGCAGTCATATCGGTACGGG | ATCTCCGCAGTCATATCGGTACGGG | ||
| - | > | + | >scaffold00003_35_36_707_F |
AAGTTGAAGGAAATGGGCGAGTTCGTGAAG | AAGTTGAAGGAAATGGGCGAGTTCGTGAAG | ||
| - | > | + | >scaffold00003_35_36_243_R |
| - | + | CAGGTTGAACATCTCCTGGACCAGCGAAC | |
| - | > | + | >scaffold00003_36_37_355_F |
CTGCTCTTCCTCGGCATGCTCATGTTC | CTGCTCTTCCTCGGCATGCTCATGTTC | ||
| - | > | + | >scaffold00003_36_37_430_R |
ATAGCCCGCCCACTGCATGACAAAC | ATAGCCCGCCCACTGCATGACAAAC | ||
| - | > | + | >scaffold00003_37_38_542_F |
CGACTGGGAGTACTACATCGGTAACACC | CGACTGGGAGTACTACATCGGTAACACC | ||
| - | > | + | >scaffold00003_37_38_565_R |
GCACTGCGTGAACTCCCTTTCCATC | GCACTGCGTGAACTCCCTTTCCATC | ||
| - | > | + | >scaffold00003_38_39_791_F |
AACTGTCGACGAAGAACCGCGTCATGAGAG | AACTGTCGACGAAGAACCGCGTCATGAGAG | ||
| - | > | + | >scaffold00003_38_39_613_R |
GTACGAGCAGTTCACCAGTTGCGTG | GTACGAGCAGTTCACCAGTTGCGTG | ||
| - | > | + | >scaffold00003_39_40_351_F |
CACTTGTCTTCTCCTCCGGATACCTGG | CACTTGTCTTCTCCTCCGGATACCTGG | ||
| - | > | + | >scaffold00003_39_40_333_R |
CTGATCCGTCATGGCGCACATTCCACGATG | CTGATCCGTCATGGCGCACATTCCACGATG | ||
| - | > | + | >scaffold00003_40_41_416_F |
| - | + | GTGGTAGGTCTGTGCCTGGTGGTTATG | |
| - | > | + | >scaffold00003_40_41_370_R |
AAGTAGTGCGGCTCCTGCCATCTTCGTTTG | AAGTAGTGCGGCTCCTGCCATCTTCGTTTG | ||
| - | > | + | >scaffold00003_41_42_419_F |
| - | + | GTAGGTCTGTGCCTGGTGCTGGTGGTTATG | |
| - | > | + | >scaffold00003_41_42_166_R |
| - | + | GTACCGCCAACACGACGACTCATTC | |
| - | > | + | >scaffold00003_42_43_317_F |
GTTCCTGTCCTCGTGCAGCACAATTCGATC | GTTCCTGTCCTCGTGCAGCACAATTCGATC | ||
| - | > | + | >scaffold00003_42_43_298_R |
| - | + | ACCGGGGATCATCGTGGTCTTGTAC | |
| - | > | + | >scaffold00003_43_44_737_F |
ATCGTTCGCAGCGGCATCGATTCAC | ATCGTTCGCAGCGGCATCGATTCAC | ||
| - | > | + | >scaffold00003_43_44_992_R |
TGAAGCTGACCAAGCTGGTGGTCTC | TGAAGCTGACCAAGCTGGTGGTCTC | ||
| - | > | + | >scaffold00003_44_45_1117_F |
| - | + | CTCTGTGAGTACGTGGAACAGGCGATG | |
| - | > | + | >scaffold00003_44_45_224_R |
AAGATCACCACGGCGTCGACACAGCTCAAC | AAGATCACCACGGCGTCGACACAGCTCAAC | ||
| - | > | + | >scaffold00003_45_46_422_F |
GTGATGCCCTCGGATCCCAGTTCTTCGTAC | GTGATGCCCTCGGATCCCAGTTCTTCGTAC | ||
| - | > | + | >scaffold00003_45_46_399_R |
AACTCGGTCAAGCTCGAGTTCCTGGTG | AACTCGGTCAAGCTCGAGTTCCTGGTG | ||
| - | > | + | >scaffold00003_46_47_258_F |
GACCTGCTGAGTGGATCATCAACCTATCG | GACCTGCTGAGTGGATCATCAACCTATCG | ||
| - | > | + | >scaffold00003_46_47_510_R |
GTTGTAACCCATGGCTGACGAAGTAGGG | GTTGTAACCCATGGCTGACGAAGTAGGG | ||
| - | > | + | >scaffold00003_47_48_427_F |
CATCACGGCGAACAACAACCAGACCTAC | CATCACGGCGAACAACAACCAGACCTAC | ||
| - | > | + | >scaffold00003_47_48_271_R |
GAACAACGGCATGAGGTCCTTGAACATGAC | GAACAACGGCATGAGGTCCTTGAACATGAC | ||
| - | > | + | >scaffold00003_48_49_532_F |
GAAGGGTCAACAGTGAGATCGCCTGGTTC | GAAGGGTCAACAGTGAGATCGCCTGGTTC | ||
| - | > | + | >scaffold00003_48_49_741_R |
TGTAGCTGCCCTTCTCCTCCTGTTC | TGTAGCTGCCCTTCTCCTCCTGTTC | ||
| - | > | + | >scaffold00003_49_50_208_F |
GTGGTGTGCATCGTGCAGATCCCGTTCTAC | GTGGTGTGCATCGTGCAGATCCCGTTCTAC | ||
| - | > | + | >scaffold00003_49_50_975_R |
| - | + | AACCCGGAGCCGAAGAGTTCGAGAAAC | |
| - | > | + | >scaffold00003_50_51_1445_F |
TGGCGTACCTGGAGAAGGTGGTTTC | TGGCGTACCTGGAGAAGGTGGTTTC | ||
| - | > | + | >scaffold00003_50_51_439_R |
CAATGGCACCCTCACATATGGCAGATAATTG | CAATGGCACCCTCACATATGGCAGATAATTG | ||
| - | > | + | >scaffold00004_1_2_381_F |
GAACACGTCACAACCGTGCTTCCAG | GAACACGTCACAACCGTGCTTCCAG | ||
| - | > | + | >scaffold00004_1_2_1879_R |
| - | + | ATCGTCTGGGTCAACACCTGGTTCCTG | |
| - | > | + | >scaffold00004_2_3_250_F |
GTTGAACCGTCTGAACGACCGAGGAAG | GTTGAACCGTCTGAACGACCGAGGAAG | ||
| - | > | + | >scaffold00004_2_3_411_R |
TGTTGTCGGGGTAGGTGGGTAAACG | TGTTGTCGGGGTAGGTGGGTAAACG | ||
| - | > | + | >scaffold00004_3_4_333_F |
TGACTTCTCCGACCAGATCGCACACCTGTC | TGACTTCTCCGACCAGATCGCACACCTGTC | ||
| - | > | + | >scaffold00004_3_4_675_R |
GAGCTTCTCCTTGTGCTGGGTCAAC | GAGCTTCTCCTTGTGCTGGGTCAAC | ||
| - | > | + | >scaffold00004_4_5_297_F |
GGGAGGTTGTAGATCACGAACTGGATGTAG | GGGAGGTTGTAGATCACGAACTGGATGTAG | ||
| - | > | + | >scaffold00004_4_5_180_R |
CAGAACGAGTCCTACAACGGGAACCTTTC | CAGAACGAGTCCTACAACGGGAACCTTTC | ||
| - | > | + | >scaffold00004_5_6_411_F |
GAGATCATGTCGCTGGGCACCAATC | GAGATCATGTCGCTGGGCACCAATC | ||
| - | > | + | >scaffold00004_5_6_469_R |
| - | + | AGCAGAGCACCCGTCTGATGTACAG | |
| - | > | + | >scaffold00004_6_7_257_F |
CGCGTTGAAAAACCGGGCGATATCC | CGCGTTGAAAAACCGGGCGATATCC | ||
| - | > | + | >scaffold00004_6_7_545_R |
GTCTTCGTGGTCAACAAGGCCGATC | GTCTTCGTGGTCAACAAGGCCGATC | ||
| - | > | + | >scaffold00004_7_8_584_F |
TGATCAACGAGGCGTGCAACGGTAG | TGATCAACGAGGCGTGCAACGGTAG | ||
| - | > | + | >scaffold00004_7_8_419_R |
ATGATCGACACCGTTACGGCGAGATGG | ATGATCGACACCGTTACGGCGAGATGG | ||
| - | > | + | >scaffold00004_8_9_447_F |
GAACCTGCTGTTCATGGTCGGTCTG | GAACCTGCTGTTCATGGTCGGTCTG | ||
| - | > | + | >scaffold00004_8_9_234_R |
ACAGTGGTCTCCGTCTACAGTCCAC | ACAGTGGTCTCCGTCTACAGTCCAC | ||
| - | > | + | >scaffold00004_9_10_1726_F |
TAGAGGGTCAACGGTTCGTCACCAG | TAGAGGGTCAACGGTTCGTCACCAG | ||
| - | > | + | >scaffold00004_9_10_313_R |
GTCAAGGATGACTGCGTGACTGGCTAC | GTCAAGGATGACTGCGTGACTGGCTAC | ||
| - | > | + | >scaffold00004_10_11_783_F |
AGAGCAGTTCGAGGATGTTGGGGTC | AGAGCAGTTCGAGGATGTTGGGGTC | ||
| - | > | + | >scaffold00004_10_11_256_R |
GCCGACTTCTACGTACTGCTGTCCATG | GCCGACTTCTACGTACTGCTGTCCATG | ||
| - | > | + | >scaffold00004_11_12_1607_F |
TACCGACAAGTGAGAGGACGACGTG | TACCGACAAGTGAGAGGACGACGTG | ||
| - | > | + | >scaffold00004_11_12_704_R |
GACCTGATCGCGGTGATTGGTTGAC | GACCTGATCGCGGTGATTGGTTGAC | ||
| - | > | + | >scaffold00004_12_13_750_F |
TCGATGGACATGCTCTCCTCCGAGATCGTC | TCGATGGACATGCTCTCCTCCGAGATCGTC | ||
| - | > | + | >scaffold00004_12_13_895_R |
| - | + | TATGCGAGCGTCCGAAAAAGATCGACCAGC | |
| - | > | + | >scaffold00004_13_14_550_F |
GAGCACATCAAGATCACTGACAATGGGATC | GAGCACATCAAGATCACTGACAATGGGATC | ||
| - | > | + | >scaffold00004_13_14_243_R |
AACACTCCGAAATCCGCTGGCTCTC | AACACTCCGAAATCCGCTGGCTCTC | ||
| - | > | + | >scaffold00004_14_15_556_F |
CTGGTGGGACTCGTTGAGCATCCAATG | CTGGTGGGACTCGTTGAGCATCCAATG | ||
| - | > | + | >scaffold00004_14_15_220_R |
CCGAGGGAAAGAACCGGACATTGTG | CCGAGGGAAAGAACCGGACATTGTG | ||
| - | > | + | >scaffold00004_15_16_1515_F |
CTTCACCGGCCTGTTCTTCGTCATCAC | CTTCACCGGCCTGTTCTTCGTCATCAC | ||
| - | > | + | >scaffold00004_15_16_167_R |
| - | + | CAGTACCTGTGGCGTCACACGCGATAAGAG | |
| - | > | + | >scaffold00004_16_17_236_F |
GGTTCGACACCAGGATTTCGAGGTCAC | GGTTCGACACCAGGATTTCGAGGTCAC | ||
| - | > | + | >scaffold00004_16_17_467_R |
GATCTGGTTCCACATCTGTCCCTGGTG | GATCTGGTTCCACATCTGTCCCTGGTG | ||
| - | > | + | >scaffold00004_17_18_420_F |
| - | + | CCTGAGGCACAAGTCAGGGTATCGATC | |
| - | > | + | >scaffold00004_17_18_720_R |
CCAACATCAAGAGCCACCACAACGTC | CCAACATCAAGAGCCACCACAACGTC | ||
| - | > | + | >scaffold00004_18_19_226_F |
GTGTGATCGACACGGTTACGTCGAAG | GTGTGATCGACACGGTTACGTCGAAG | ||
| - | > | + | >scaffold00004_18_19_492_R |
| - | + | TTAACAGGGGGCCCTTCCTTCAACACGGTC | |
| - | > | + | >scaffold00004_19_20_273_F |
GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC | GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC | ||
| - | > | + | >scaffold00004_19_20_878_R |
GACGAGCAACTGGACTCGCTGAACAAG | GACGAGCAACTGGACTCGCTGAACAAG | ||
| - | > | + | >scaffold00004_20_21_2075_F |
| - | + | AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG | |
| - | > | + | >scaffold00004_20_21_521_R |
TGAAGATCACCCAGGATCTCAAGGAGTACG | TGAAGATCACCCAGGATCTCAAGGAGTACG | ||
| - | > | + | >scaffold00004_21_22_386_F |
GAAGACTTCGCCACCGAATACCTCAGC | GAAGACTTCGCCACCGAATACCTCAGC | ||
| - | > | + | >scaffold00004_21_22_182_R |
TTCGACGTACACACCACCCTCGCCGTAATC | TTCGACGTACACACCACCCTCGCCGTAATC | ||
| - | > | + | >scaffold00004_22_23_594_F |
AATTCAGCGGACAGCGGTACGAGGGTGTAG | AATTCAGCGGACAGCGGTACGAGGGTGTAG | ||
| - | > | + | >scaffold00004_22_23_1225_R |
AACAGGACCTGCTCGACAACAAGATCAAG | AACAGGACCTGCTCGACAACAAGATCAAG | ||
| - | > | + | >scaffold00004_23_24_720_F |
| - | + | TCCACCGGAACATGGGTGATACTCGAC | |
| - | > | + | >scaffold00004_23_24_552_R |
GTTGCCTTTGCACAGGTAGATCCCG | GTTGCCTTTGCACAGGTAGATCCCG | ||
| - | > | + | >scaffold00004_24_25_667_F |
CAAGCCATGTCGAAGCAGCACTACTC | CAAGCCATGTCGAAGCAGCACTACTC | ||
| - | > | + | >scaffold00004_24_25_322_R |
AAGCACCTGCTGTTCACAAGCGAACTGATC | AAGCACCTGCTGTTCACAAGCGAACTGATC | ||
| - | > | + | >scaffold00004_25_26_542_F |
GCAGATCGACGAGTACCTGGAGTTCTAC | GCAGATCGACGAGTACCTGGAGTTCTAC | ||
| - | > | + | >scaffold00004_25_26_247_R |
| - | + | AGGAGTCCGACCATGAAGATCAGGAAGATC | |
| - | > | + | >scaffold00004_26_27_255_F |
GATCGTTCGACTACCCACAATCCGCAGATC | GATCGTTCGACTACCCACAATCCGCAGATC | ||
| - | > | + | >scaffold00004_26_27_671_R |
GGTAGAGCAGCCATTCCAGTTCCTGTG | GGTAGAGCAGCCATTCCAGTTCCTGTG | ||
| - | > | + | >scaffold00004_27_28_577_F |
CTGTTCACCGAGGAGGGCGAGTTCTTCTTC | CTGTTCACCGAGGAGGGCGAGTTCTTCTTC | ||
| - | > | + | >scaffold00004_27_28_485_R |
GAAAACGGGGTGTTCCAGCTTCATCGCTTC | GAAAACGGGGTGTTCCAGCTTCATCGCTTC | ||
| - | > | + | >scaffold00004_28_29_1409_F |
AACCGACACACCTGCAGTTGCCGTACCAAC | AACCGACACACCTGCAGTTGCCGTACCAAC | ||
| - | > | + | >scaffold00004_28_29_207_R |
TACTCCGGGATAAGCAAGGCTGAGCTG | TACTCCGGGATAAGCAAGGCTGAGCTG | ||
| - | > | + | >scaffold00004_29_30_278_F |
CATTTGTGACACCTGTCCAGCAGTACG | CATTTGTGACACCTGTCCAGCAGTACG | ||
| - | > | + | >scaffold00004_29_30_264_R |
TCTGGTGAGCCGACAGGTATTCCTC | TCTGGTGAGCCGACAGGTATTCCTC | ||
| - | > | + | >scaffold00004_30_31_444_F |
ACCTTCTTGCTCTTACGGCCCACCTTG | ACCTTCTTGCTCTTACGGCCCACCTTG | ||
| - | > | + | >scaffold00004_30_31_739_R |
| - | + | GAGTACGTCAAGTTCATTTTCGCCACCAC | |
Revision as of 12:57, 3 November 2010
Contents |
Primer Naming Format
- >[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse]
ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R
5' contig1 3' contig2
--------CTCAGGTCAGGCAGGCGTGATGATCTCTTC----------------NNNNNNNNN--------------------------------------
TACTAACCTCGGGTCGGCGTGAGAGGAGATTG
3' 5'
1105bp 529bp
List of primer
- By Using Primer3
- "Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer).
primer part 1 - scaffold00001
>scaffold00001_1_2_1105_F CTCAGGTCAGGCAGGCGTGATGATCTCTTC >scaffold00001_1_2_529_R TACTAACCTCGGGTCGGCGTGAGAGGAGATTG >scaffold00001_2_3_751_F CAGAACTGGAGAACGTCCATGCTCGAGAAC >scaffold00001_2_3_1360_R TCGATCACCAGCTCACGGAAGTCCTCGTTG >scaffold00001_3_4_586_F GTCAGTGCCGATCAGGATGTTGTCCAC >scaffold00001_3_4_183_R ATCAACTGCCAGAACTCGTCGGTCCTCATG >scaffold00001_4_5_568_F TACGTCGAGGAACTGGGCGACTTCAAGACC >scaffold00001_4_5_838_R CATGTCGTCGATCTGCACGTTGACCAG >scaffold00001_5_6_1090_F GTTGAAGAAGCTGACCAGTCCGACGAGTAC >scaffold00001_5_6_374_R CGGGTCTTCTTCGAGATGGACATCTACTG >scaffold00001_6_7_939_F ATCAGCGGTTACGTCTGGTGGTGAC >scaffold00001_6_7_870_R CGATCTCCACGTTTGACAGGATGCTCTG >scaffold00001_7_8_818_F GAACTGGAGTTGATGGAGCAGCGCGAAAC >scaffold00001_7_8_545_R ATCAGTTTGATCGGCGAGACGTGCGAGACC >scaffold00001_8_9_346_F CTCGTGCTGATCGTCGAGTACGCCATCTTC >scaffold00001_8_9_358_R ATGGACATGGTCCACCTGGTCATCG >scaffold00001_9_10_1135_F AGACCGGATTGAGTGCTTCCTCTGCTGGTG >scaffold00001_9_10_1408_R CGACCAATTTCCGACGAGGTGACATTC >scaffold00001_10_11_533_F TTCGCCACCACCGGTCTAGGATGAGAGATC >scaffold00001_10_11_191_R ATTCCTCGCGCAGTTGACCCAGTTC >scaffold00001_11_12_354_F AAGGTCAGGATGGCGGTGAACACCTTGGTC >scaffold00001_11_12_345_R ATGTTGTCGCTGACCTTCGCGATACGGGAG >scaffold00001_12_13_1313_F TTTCAGGCTTTGAGGATCGAGGTAAACAGG >scaffold00001_12_13_315_R TCGCATCTTCTCAGCCAGTTCCGAG >scaffold00001_13_14_961_F GTCATCGGACAGCCGATTCTTCGACGACTG >scaffold00001_13_14_1221_R AGTCGTCGAAGCGCAGAAGGTACATGTAGG >scaffold00001_14_15_1407_F CTTGTTTCGATAGCATTACCGCCCAGTAAC >scaffold00001_14_15_533_R GTACTCGTCGAAGGTGGACACGTTCACTC >scaffold00001_15_16_126613_F Can't find proper primer >scaffold00001_15_16_569_R CTTCTACCACGACACACACACCGATCC >scaffold00001_16_17_1785_F CTCGAAGACGTCAACAACCACACCAATCTG >scaffold00001_16_17_478_R CTCGTAACTCTCCATCGGTTCTCCAGTAAC >scaffold00001_17_18_225_F GATCGTGACGAACGTGAAGGTCCAC >scaffold00001_17_18_408_R CAGATCCGGTACAGCTCCTTGTATTCCAG >scaffold00001_18_19_320_F CAGATAGATGGTGAAGTCAGGACGACGG >scaffold00001_18_19_177_R ATGAACTTTCCACCGATGCCGGTCGTCTTC >scaffold00001_19_20_313_F GTGTCGTTGTAGGCGATGAGGAGTTGGTG >scaffold00001_19_20_645_R CTGTCTACAACATCCCCGTCGCACTCCAAC >scaffold00001_20_21_312_F TGTCGTTGTAGGCGACGAGGAGCTGATCAC >scaffold00001_20_21_428_R TCGGACATGTCTTCGACCTGAGTGC >scaffold00001_21_22_243_F AAGGACGGACGTGGACATCGATCAGCAATC >scaffold00001_21_22_1379_R AGGACTCCTGTACGTCGATCACGACGAGTG >scaffold00001_22_23_238_F GTTTCACTCATCGGTGTACCCTCCCGATC >scaffold00001_22_23_244_R GAAGTTCGTCATCGAGAACGTCTCCGTGC >scaffold00001_23_24_410_F ACTGACCACGGTGCAGCTCTACTTCAC >scaffold00001_23_24_1184_R AGCGTGTAGTCGGCGGTATACACCTTC >scaffold00001_24_25_444_F ATTCGACGGGCAGGACGTCAACAAG >scaffold00001_24_25_458_R TAGGTGTGCTCCAGGTAGGTGAGGAAC >scaffold00001_25_26_203_F GTCGAGGGGATCAACACAAGTGGAATCAAG >scaffold00001_25_26_184_R GTACCGTCGACAAGCAACTCCTGATCGTC >scaffold00001_26_27_369_F ATGAGCGAGCAGGTCTTCCTCCTAC >scaffold00001_26_27_706_R GAACACGCCGTAGTTGTTCAGCGTGAAG >scaffold00001_27_28_280_F TGACGTGAGGGGACTACGGATGTAC >scaffold00001_27_28_260_R ACTTCGACAGGTAGGCCTTGCAGAG >scaffold00001_28_29_209_F TCTACGGTGGGTTGCACTGGTTTAGAGAAG >scaffold00001_28_29_357_R TACAGCTGAAGGATCTTCTCGTCGAGGACC >scaffold00001_29_30_1643_F CCGATCCAGGCGAAGATCAGATTGGTC >scaffold00001_29_30_767_R ATCGACATAGCGCTCCAACAGATCGGTGAC >scaffold00001_30_31_237_F CACCACACGTTGTACGTCTTCGTGCTCTAC >scaffold00001_30_31_286_R GACATCCCCCAAGTCTTTCCTCTCATGCTC >scaffold00001_31_32_566_F AAACGAGCCGTACAACGGGCAACTG >scaffold00001_31_32_169_R ATCTTCCTGACCTCGTCGGTGTGGTACTTC >scaffold00001_32_33_266_F AACATTGACAGGGTGGCACGCAGTTCG >scaffold00001_32_33_169_R TTGTCAATGTAGCGTTGACGGGTGCCTGAC >scaffold00001_33_34_795_F GTCGACGACGAGAATCCGGTTCATG >scaffold00001_33_34_365_R GTTCCCTGCTGCTGAACTGGTTCTTCAC >scaffold00001_34_35_1071_F CAGTACGTCATCTTCGACCGGACCATTTG >scaffold00001_34_35_2096_R AGTCGTCGTCGTCGAACATCGTGAC >scaffold00001_35_36_685_F GTCGTTCAGGTTCAGGTAGAACTCGGTGAC >scaffold00001_35_36_0_R Can't find proper primer >scaffold00001_36_37_1073_F GATCGGTTTGCCGCGGTCGTTGTATTCGAC >scaffold00001_36_37_740_R ATACGGCTTCGTCGAGAACCTGACC >scaffold00001_37_38_498_F TTCGACGAACTGTGGCAACGCTTCTCC >scaffold00001_37_38_562_R AGGACGTCATGCACGGTCAACACAC >scaffold00001_38_39_612_F AACATGTCGTTGAGCGCGGCCAGTTTG >scaffold00001_38_39_672_R CTCTTCGACGAGATCATCCAGGTGCTG >scaffold00001_39_40_196_F CACAATTTCATCGATCAACACAAACGCGAG >scaffold00001_39_40_425_R ACCTGCAACTGACTCGGCTGGCTATAGCTG >scaffold00001_40_41_522_F GACTACAAGCCGGAGAACGTGCTCATCTC >scaffold00001_40_41_660_R GTGCAGAAGGGATAGAGGGTGACGTAC >scaffold00001_41_42_205_F ACCGAAGCAGTCGAAGACGGTACAC >scaffold00001_41_42_339_R GTCTTCTTCTGGCCCCGCAACGAGATCATG >scaffold00001_42_43_389_F TAAAATCTGTGATCTGGGCTAACCTTGGGG >scaffold00001_42_43_223_R GTGATCGGTGTGTTCGTGGCTGACCAGTAC >scaffold00001_43_44_440_F CTCGGAGGAAAACGGTGAACAGTCAGCAAC >scaffold00001_43_44_563_R TGAACGCCTCCACCCAGCATTTGATCACAG >scaffold00001_44_45_93801_F Can't find proper primer >scaffold00001_44_45_485_R GTGTTGCGATCGGATTCGTAGCTGAC >scaffold00001_45_46_401_F GGATCAGCTCCGGATAGTTGAAGTCGTC >scaffold00001_45_46_423_R ACAACGAGGACTTCGACAACGGCTTCAAGG >scaffold00001_46_47_370_F GTAAACTCCTGATCTCAGCCGCTCTCGTG >scaffold00001_46_47_845_R CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG >scaffold00001_47_48_1970_F GACGACTACCTGCGTAAACCCTTCGACCTG >scaffold00001_47_48_1044_R CACCAATCTGCTTGTGGGTCTGGTGCTGAC >scaffold00001_48_49_1639_F GATGTTCATCGACCTGGAGAACGTGC >scaffold00001_48_49_244_R CATCTCTCCCGTCAGCTCATCGTTCGAAG >scaffold00001_49_50_1297_F CAGGGTTTCCTGAGCCACCTTGAAC >scaffold00001_49_50_822_R CTGTTCTCCCAGGACCCGATCTTCAACTC >scaffold00001_50_51_227_F CGTGCCATGTTCTGAACGAGAACGATC >scaffold00001_50_51_860_R GTGACCAGCGAGTGAATCTTGTCGCGTTG >scaffold00001_51_52_1410_F GATGTCCACGTCGTACGCCATGCTGTTGTC >scaffold00001_51_52_509_R CCTGATTCACCTGATTCGTCCACCAGTTC >scaffold00001_52_53_361_F GTCATCCACACATGGCATTCTGTGACC >scaffold00001_52_53_353_R AGGATGGCGTAATCCGCGATGATGG >scaffold00001_53_54_414_F TTCTGGAAGACCATCTGCAGACCGATCTCG >scaffold00001_53_54_823_R TACGCGAAGGGACGTTAGAGATGGCAC >scaffold00001_54_55_1250_F CTGGAACCGGATGATGTTGGAGTCCTC >scaffold00001_54_55_175_R TACCGAGGAACTGATCGACCTCTCC >scaffold00001_55_56_239_F CACCTGACCTCTGACAATGAACGCCGAAAG >scaffold00001_55_56_206_R GATTGGCTTCTATACAAACTAAGGTGGCGC >scaffold00001_56_57_412_F GTACTCACGATGGTCCTTCTCCTCGTTG >scaffold00001_56_57_184_R CAAACCGGTGGTCGGGCTGATCATGTATTC >scaffold00001_57_58_1204_F TCATGCTGTCCGTATCCATTTAGGACCTTC >scaffold00001_57_58_939_R TTCTACCGTGCCTACTTCGAGACCGAC >scaffold00001_58_59_788_F CAAAGCAAATCGGAGGGGACATGACG >scaffold00001_58_59_229_R ACACGTTCGTCCGCAACAACGGATG >scaffold00001_59_60_1979_F GTGACCAGCCAGGTCTGTTCGAATCTCAC >scaffold00001_59_60_625_R GAGATCATGTACGCCGACTTCAGCTTCGTG >scaffold00001_60_61_755_F GTACGGTTTCTGACCAACACCGACTCGAAG >scaffold00001_60_61_647_R CCAACATCACCATCACGAACCTCGG >scaffold00001_61_62_285_F TCAGGTTGAGATCGACCATCGCGAG >scaffold00001_61_62_944_R TTGCACCATCAGCTCAGCATCGTCTTCGAC >scaffold00001_62_63_898_F CTGATGATCAAGGTGTTGCGGCTGTC >scaffold00001_62_63_367_R GTTGTAGCCGCCGAAGTCGTTCATGCTGAC >scaffold00001_63_64_711_F ACGTAGTTCCCGACCGTAGAGCAGTTC >scaffold00001_63_64_1286_R CGATAGGTGAGACTCGATGCATGGTTAGTG >scaffold00001_64_65_324_F CTGGTGTGCTTTTCGGTTCGGGTAC >scaffold00001_64_65_297_R AACGCCCACGTCATCCTCGAAGAAC >scaffold00001_65_66_1986_F GATCAGTCCGGCGATCGACGAGTAGAGAAC >scaffold00001_65_66_1292_R GTTGCTGCTGGAAGTGGCATGGGAAAC >scaffold00001_66_67_428_F CTTCGTCACTGGCTGTGTCAAGGTCAAC >scaffold00001_66_67_346_R ACTGTTCCTGCTGTACTCCTCGGTG >scaffold00001_67_68_5122_F Can't find proper primer >scaffold00001_67_68_0_R Can't find proper primer >scaffold00001_68_69_5187_F Can't find proper primer >scaffold00001_68_69_1373_R CTCCTGGTGGGCTCGGTGAAAACCAACATC >scaffold00001_69_70_254_F CTTCGACATGGAGGCAGTGCTTGAGAACTC >scaffold00001_69_70_318_R AGTGAGGAGGAACAGTTCTACAATGGTGTG >scaffold00001_70_71_240_F CATTCGGCGTGGTAAAGACCCACTGGTAC >scaffold00001_70_71_233_R ATCGATGACCCTCCAAGACAGACCTCATTG >scaffold00001_71_72_349_F AACCCACGCATCCCACTCAACGAGCTGAAC >scaffold00001_71_72_0_R Can't find proper primer >scaffold00001_72_73_2964_F Can't find proper primer >scaffold00001_72_73_0_R Can't find proper primer >scaffold00001_73_74_2968_F Can't find proper primer >scaffold00001_73_74_0_R Can't find proper primer >scaffold00001_74_75_356_F GTGTTCACCGGCGTGATGTACAACGACTAC >scaffold00001_74_75_0_R Can't find proper primer >scaffold00001_75_76_788_F GTCGACGTTTCATCACGTCACTGGGTG >scaffold00001_75_76_0_R Can't find proper primer >scaffold00001_76_77_1854_F GTGATCAAGGTGATCGAAGCCCTGC >scaffold00001_76_77_535_R ATCTACAACCACCTGGGCGGCAAGGAGAAC >scaffold00001_77_78_624_F AATCTCATGGGGTGCCTTCGTCGATCGGTGAG >scaffold00001_77_78_349_R CTCGGCAACCGACTGTGGAACAACATC >scaffold00001_78_79_195_F GTAGTTGCACGAGACGGCCATTACCTTG >scaffold00001_78_79_276_R GAAATGCCGTCACTCTCCGTAGATGTCC >scaffold00001_79_80_301_F TACGTTTGGCTCAACACCCCACGCGAGATG >scaffold00001_79_80_212_R GTCGCTGATCATGGTTCGGTAGACGATGTC >scaffold00001_80_81_426_F CACTGCCCAAACCGATCTATCCGACGTTC >scaffold00001_80_81_202_R GATCCAAGCTGTCTTAACGAGGAGTTGCC >scaffold00001_81_82_751_F CAAATCCGGGCACCTCGAACTCAAG >scaffold00001_81_82_233_R AACACCACCACCCCCGAGTAGCAGACAACAAC >scaffold00001_82_83_253_F CAAGGTCATCGTCAGCGGTGGATTG >scaffold00001_82_83_302_R AGATCCGGATACTGCGCTGGGAAAC >scaffold00001_83_84_243_F GTGGGGCAAGTGCTGGAGTTTGATTCAC >scaffold00001_83_84_182_R GTGGACGGACCAGTCGTGATGTTCGATTC >scaffold00001_84_85_414_F GTTTACCCTCGATCCGGCAGATGAAGC >scaffold00001_84_85_726_R GACCCGTCCAACACCTTCGATCAGAGCTTC >scaffold00001_85_86_202_F GTTTAGGAAGTGTGGACACGGCCGGAAGTC >scaffold00001_85_86_191_R CCGAAGTGTGCAAACGTTGTCTTTACGTC >scaffold00001_86_87_218_F TCAACAACCCAGTGAACACCGAGGACGTAC >scaffold00001_86_87_245_R CATTGAGAAAGCGATGTACCGCCACGAGAC >scaffold00001_87_88_204_F GAAGTGATCACGCGATCCACGGGATTCCAC >scaffold00001_87_88_267_R AGCTTCCAGACCCTGGTCAACAGTC >scaffold00001_88_89_1973_F AGTAGCCGATGACCCCGTTGAGTTG >scaffold00001_88_89_463_R CAGGTGTTCAACCTCGCCCAGACCTTCTAC >scaffold00001_89_90_613_F ATGACCTTCGTGAAACTGACCTTCTCGCTG >scaffold00001_89_90_528_R GACAAGGACTCCGCCAAGAAGGACGAATC
primer part 2 - scaffold00003, scaffold00004
>scaffold00003_1_2_276_F GACGTGTACATCTCGAACATCTCGCGG >scaffold00003_1_2_387_R AAAACGGGCGTTCACCGCATGGTAC >scaffold00003_2_3_232_F CAAGGGAGAGTTCCGTTACGACCACAG >scaffold00003_2_3_460_R TTGATCAGCCGGCACAGCAACAAGGTGGTG >scaffold00003_3_4_218_F ACAAGCTCATTGCCTACTAGATCGACTTCG >scaffold00003_3_4_1054_R GCATCGTCCAACGGTAGCCTTCTTTC >scaffold00003_4_5_574_F ATCGACCTGGTGCACGCGAACAACTCCAAG >scaffold00003_4_5_289_R GAAGAAGGCGTTGGAGTGCCACAGTTG >scaffold00003_5_6_195_F ATCTGTTGCCGAATTGGCCTGTACTAGATC >scaffold00003_5_6_263_R ATGGCATCCCTCAACCACTACCACC >scaffold00003_6_7_918_F ATGCTCAGTCCGGGAGTACAGCTCGGTAAC >scaffold00003_6_7_245_R GCATGCTGTTGTTGATGCTGTCCTTCAG >scaffold00003_7_8_365_F ATCCGACGAGTACGACATCCACCTG >scaffold00003_7_8_601_R CAGACCAACTACGCCACAAGCAAGG >scaffold00003_8_9_472_F CTGTTCCAGACCTTCCTCACCGTGCTTGAC >scaffold00003_8_9_218_R CTTATGCCCTGACAGAAGGTGCAAGATCAG >scaffold00003_9_10_376_F CATGCACAAGCAGGACCTCATCGAG >scaffold00003_9_10_212_R TTTCCGTTGAGCCGCCACTCGTACTTGTAG >scaffold00003_10_11_1436_F TGGTCCTGCCGTACTTCCTGATCACCACTG >scaffold00003_10_11_232_R GATGGGACAGACTTCGTACACCGGTTG >scaffold00003_11_12_266_F CTGTATTGATGATCCACTCGCTGCCGCTC >scaffold00003_11_12_1184_R TACAGTGATCGGCCGTTGTTGGAGTGTGTG >scaffold00003_12_13_2070_F CTCCAGCAGGGTTTCATCGATTCGGTAG >scaffold00003_12_13_324_R CTGGACCAGTTCACCCTCCAGATCATG >scaffold00003_13_14_463_F CTGGTGTCACACAACGAAAAGGACCTGAC >scaffold00003_13_14_304_R GAAGAAGTCGTCCTTCTCCTTGACCGACAG >scaffold00003_14_15_137305_F Can't find proper primer >scaffold00003_14_15_518_R AATACCGCTACGGTGCTGGAACCGATGTAC >scaffold00003_15_16_227_F GTTTCTGGACAAGTACGGTTTCGGGACC >scaffold00003_15_16_170_R CTTGAGGCGCTCACTGATGCACTTAAC >scaffold00003_16_17_319_F TCACGTGATCTCCTGATAGGTAGACAGACG >scaffold00003_16_17_503_R TACCTGTGGCCCCAAAGCTACTACCTG >scaffold00003_17_18_314_F TCTGAACGAGACGGAATGGGAGATCCTCAC >scaffold00003_17_18_222_R GACATCACCATCCAGGCAGAGAACATGCTG >scaffold00003_18_19_746_F GTTGCTTCGAATCTGGTGACGGGAG >scaffold00003_18_19_471_R GTATACCGCAACATCGGCGAAATGAAGTC >scaffold00003_19_20_655_F GAAGGAACGGCAACTTTCGGTCACGGTAC >scaffold00003_19_20_485_R CAAGGAGACCGTCTACAAGATCTGGCATC >scaffold00003_20_21_294_F ATCAGCCCCGAGCAATTGACCGACCTGAAC >scaffold00003_20_21_200_R GAAGATCGGCAGCTCCATCGTCTCATC >scaffold00003_21_22_1034_F GAAGTAACCGGTGGTGTTTTCGGCCAG >scaffold00003_21_22_354_R ATCTCGCCGGTTACAAGCAGATCCTC >scaffold00003_22_23_1299_F TTCAGATGGTTAAGGACAGCTTCATGAGCG >scaffold00003_22_23_368_R ACGTGATCGGTGGGAACTTCGACTC >scaffold00003_23_24_235_F CCCAGGGAATATAACGATGGTTCAGGTCAG >scaffold00003_23_24_1163_R GAACTGCTGGTTCATCGAATGGTCGTTGAC >scaffold00003_24_25_268_F CTCGAAGTCCCGGTCAAGAAGATCCTCAC >scaffold00003_24_25_666_R TATCGTTCGCGTCGATGGCCGACAACATTG >scaffold00003_25_26_360_F GAAAGGCTGACCATCTACCCGGAGTAC >scaffold00003_25_26_636_R TGATCCACGTGTCCGTACATCATCGTG >scaffold00003_26_27_1270_F GAAATCAAGATCCTGAACCAGTCCCGGAAG >scaffold00003_26_27_244_R CCCGATTCCTTGTCCTGGAACAGATAGAAG >scaffold00003_27_28_833_F GTACTCGCCGACTTTCCCGTTCTCATC >scaffold00003_27_28_474_R GTACACCTCGTGCTCGGGACACAAATGGTC >scaffold00003_28_29_210_F GTACACGTGCCGTTCCCGTTTCTGGTGAAC >scaffold00003_28_29_173_R ACGGCGTCACGAACAACACCTACTG >scaffold00003_29_30_703_F TCAGCGATTCAGAGGTCCCGATCAGAGGTC >scaffold00003_29_30_1341_R TACCTGCATACCGTACGTGGAGTCG >scaffold00003_30_31_256_F TTCCGGGGATGAGTAGCCGACCAATTGTCC >scaffold00003_30_31_1239_R GATCACCGGTTGGTAGACCAGATCCAG >scaffold00003_31_32_351_F CACAAATTGAACTTGAGCTGGGATGTCAGG >scaffold00003_31_32_565_R CAGGATGACAGTGTGAGTCAGCCATGGAC >scaffold00003_32_33_376_F AGATCGACGAGCTCAACGTCTATCCG >scaffold00003_32_33_1069_R GTCATTCGACACCCACCAGCAACGGATAG >scaffold00003_33_34_1969_F GTCCTCAAGCATCGCAAGCGCAAGGAAAAG >scaffold00003_33_34_485_R CTCCAACTGCTCGGAGAGGAACTTGTAAC >scaffold00003_34_35_491_F GAACGAGACCAGGAGACATAGTGGCGAGTC >scaffold00003_34_35_222_R ATCTCCGCAGTCATATCGGTACGGG >scaffold00003_35_36_707_F AAGTTGAAGGAAATGGGCGAGTTCGTGAAG >scaffold00003_35_36_243_R CAGGTTGAACATCTCCTGGACCAGCGAAC >scaffold00003_36_37_355_F CTGCTCTTCCTCGGCATGCTCATGTTC >scaffold00003_36_37_430_R ATAGCCCGCCCACTGCATGACAAAC >scaffold00003_37_38_542_F CGACTGGGAGTACTACATCGGTAACACC >scaffold00003_37_38_565_R GCACTGCGTGAACTCCCTTTCCATC >scaffold00003_38_39_791_F AACTGTCGACGAAGAACCGCGTCATGAGAG >scaffold00003_38_39_613_R GTACGAGCAGTTCACCAGTTGCGTG >scaffold00003_39_40_351_F CACTTGTCTTCTCCTCCGGATACCTGG >scaffold00003_39_40_333_R CTGATCCGTCATGGCGCACATTCCACGATG >scaffold00003_40_41_416_F GTGGTAGGTCTGTGCCTGGTGGTTATG >scaffold00003_40_41_370_R AAGTAGTGCGGCTCCTGCCATCTTCGTTTG >scaffold00003_41_42_419_F GTAGGTCTGTGCCTGGTGCTGGTGGTTATG >scaffold00003_41_42_166_R GTACCGCCAACACGACGACTCATTC >scaffold00003_42_43_317_F GTTCCTGTCCTCGTGCAGCACAATTCGATC >scaffold00003_42_43_298_R ACCGGGGATCATCGTGGTCTTGTAC >scaffold00003_43_44_737_F ATCGTTCGCAGCGGCATCGATTCAC >scaffold00003_43_44_992_R TGAAGCTGACCAAGCTGGTGGTCTC >scaffold00003_44_45_1117_F CTCTGTGAGTACGTGGAACAGGCGATG >scaffold00003_44_45_224_R AAGATCACCACGGCGTCGACACAGCTCAAC >scaffold00003_45_46_422_F GTGATGCCCTCGGATCCCAGTTCTTCGTAC >scaffold00003_45_46_399_R AACTCGGTCAAGCTCGAGTTCCTGGTG >scaffold00003_46_47_258_F GACCTGCTGAGTGGATCATCAACCTATCG >scaffold00003_46_47_510_R GTTGTAACCCATGGCTGACGAAGTAGGG >scaffold00003_47_48_427_F CATCACGGCGAACAACAACCAGACCTAC >scaffold00003_47_48_271_R GAACAACGGCATGAGGTCCTTGAACATGAC >scaffold00003_48_49_532_F GAAGGGTCAACAGTGAGATCGCCTGGTTC >scaffold00003_48_49_741_R TGTAGCTGCCCTTCTCCTCCTGTTC >scaffold00003_49_50_208_F GTGGTGTGCATCGTGCAGATCCCGTTCTAC >scaffold00003_49_50_975_R AACCCGGAGCCGAAGAGTTCGAGAAAC >scaffold00003_50_51_1445_F TGGCGTACCTGGAGAAGGTGGTTTC >scaffold00003_50_51_439_R CAATGGCACCCTCACATATGGCAGATAATTG >scaffold00004_1_2_381_F GAACACGTCACAACCGTGCTTCCAG >scaffold00004_1_2_1879_R ATCGTCTGGGTCAACACCTGGTTCCTG >scaffold00004_2_3_250_F GTTGAACCGTCTGAACGACCGAGGAAG >scaffold00004_2_3_411_R TGTTGTCGGGGTAGGTGGGTAAACG >scaffold00004_3_4_333_F TGACTTCTCCGACCAGATCGCACACCTGTC >scaffold00004_3_4_675_R GAGCTTCTCCTTGTGCTGGGTCAAC >scaffold00004_4_5_297_F GGGAGGTTGTAGATCACGAACTGGATGTAG >scaffold00004_4_5_180_R CAGAACGAGTCCTACAACGGGAACCTTTC >scaffold00004_5_6_411_F GAGATCATGTCGCTGGGCACCAATC >scaffold00004_5_6_469_R AGCAGAGCACCCGTCTGATGTACAG >scaffold00004_6_7_257_F CGCGTTGAAAAACCGGGCGATATCC >scaffold00004_6_7_545_R GTCTTCGTGGTCAACAAGGCCGATC >scaffold00004_7_8_584_F TGATCAACGAGGCGTGCAACGGTAG >scaffold00004_7_8_419_R ATGATCGACACCGTTACGGCGAGATGG >scaffold00004_8_9_447_F GAACCTGCTGTTCATGGTCGGTCTG >scaffold00004_8_9_234_R ACAGTGGTCTCCGTCTACAGTCCAC >scaffold00004_9_10_1726_F TAGAGGGTCAACGGTTCGTCACCAG >scaffold00004_9_10_313_R GTCAAGGATGACTGCGTGACTGGCTAC >scaffold00004_10_11_783_F AGAGCAGTTCGAGGATGTTGGGGTC >scaffold00004_10_11_256_R GCCGACTTCTACGTACTGCTGTCCATG >scaffold00004_11_12_1607_F TACCGACAAGTGAGAGGACGACGTG >scaffold00004_11_12_704_R GACCTGATCGCGGTGATTGGTTGAC >scaffold00004_12_13_750_F TCGATGGACATGCTCTCCTCCGAGATCGTC >scaffold00004_12_13_895_R TATGCGAGCGTCCGAAAAAGATCGACCAGC >scaffold00004_13_14_550_F GAGCACATCAAGATCACTGACAATGGGATC >scaffold00004_13_14_243_R AACACTCCGAAATCCGCTGGCTCTC >scaffold00004_14_15_556_F CTGGTGGGACTCGTTGAGCATCCAATG >scaffold00004_14_15_220_R CCGAGGGAAAGAACCGGACATTGTG >scaffold00004_15_16_1515_F CTTCACCGGCCTGTTCTTCGTCATCAC >scaffold00004_15_16_167_R CAGTACCTGTGGCGTCACACGCGATAAGAG >scaffold00004_16_17_236_F GGTTCGACACCAGGATTTCGAGGTCAC >scaffold00004_16_17_467_R GATCTGGTTCCACATCTGTCCCTGGTG >scaffold00004_17_18_420_F CCTGAGGCACAAGTCAGGGTATCGATC >scaffold00004_17_18_720_R CCAACATCAAGAGCCACCACAACGTC >scaffold00004_18_19_226_F GTGTGATCGACACGGTTACGTCGAAG >scaffold00004_18_19_492_R TTAACAGGGGGCCCTTCCTTCAACACGGTC >scaffold00004_19_20_273_F GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC >scaffold00004_19_20_878_R GACGAGCAACTGGACTCGCTGAACAAG >scaffold00004_20_21_2075_F AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG >scaffold00004_20_21_521_R TGAAGATCACCCAGGATCTCAAGGAGTACG >scaffold00004_21_22_386_F GAAGACTTCGCCACCGAATACCTCAGC >scaffold00004_21_22_182_R TTCGACGTACACACCACCCTCGCCGTAATC >scaffold00004_22_23_594_F AATTCAGCGGACAGCGGTACGAGGGTGTAG >scaffold00004_22_23_1225_R AACAGGACCTGCTCGACAACAAGATCAAG >scaffold00004_23_24_720_F TCCACCGGAACATGGGTGATACTCGAC >scaffold00004_23_24_552_R GTTGCCTTTGCACAGGTAGATCCCG >scaffold00004_24_25_667_F CAAGCCATGTCGAAGCAGCACTACTC >scaffold00004_24_25_322_R AAGCACCTGCTGTTCACAAGCGAACTGATC >scaffold00004_25_26_542_F GCAGATCGACGAGTACCTGGAGTTCTAC >scaffold00004_25_26_247_R AGGAGTCCGACCATGAAGATCAGGAAGATC >scaffold00004_26_27_255_F GATCGTTCGACTACCCACAATCCGCAGATC >scaffold00004_26_27_671_R GGTAGAGCAGCCATTCCAGTTCCTGTG >scaffold00004_27_28_577_F CTGTTCACCGAGGAGGGCGAGTTCTTCTTC >scaffold00004_27_28_485_R GAAAACGGGGTGTTCCAGCTTCATCGCTTC >scaffold00004_28_29_1409_F AACCGACACACCTGCAGTTGCCGTACCAAC >scaffold00004_28_29_207_R TACTCCGGGATAAGCAAGGCTGAGCTG >scaffold00004_29_30_278_F CATTTGTGACACCTGTCCAGCAGTACG >scaffold00004_29_30_264_R TCTGGTGAGCCGACAGGTATTCCTC >scaffold00004_30_31_444_F ACCTTCTTGCTCTTACGGCCCACCTTG >scaffold00004_30_31_739_R GAGTACGTCAAGTTCATTTTCGCCACCAC
