Primer Verrucosispora gap

From CSBLwiki

Revision as of 05:11, 24 January 2011

Primer Naming Format

Scf&SolCtg primer format

Gap primer format

• >[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse]

ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R

                    CONTIG 1                                                     CONTIG 2
                               ------>
        5'                           3'  
--------CTCAGGTCAGGCAGGCGTGATGATCTCTTC----------------NNNNNNNNN----------------------------------------
                                                                       GTTAGAGGAGAGTGCGGCTGGGCTCCAATCAT
                                                                       3'                             5'
                                                                       <-------

                                            1105bp               529bp

List of primer

primers of scaffold & sole contigs

scf1

scf2

scf3

scf4

ctg205

ctg231

• By Using Primer3
• "Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer).

primer part 1 - scaffold00001

>scaffold00001_1_2_1105_F
CTCAGGTCAGGCAGGCGTGATGATCTCTTC
>scaffold00001_1_2_529_R
TACTAACCTCGGGTCGGCGTGAGAGGAGATTG
>scaffold00001_2_3_751_F
CAGAACTGGAGAACGTCCATGCTCGAGAAC
>scaffold00001_2_3_1360_R
TCGATCACCAGCTCACGGAAGTCCTCGTTG
>scaffold00001_3_4_586_F
GTCAGTGCCGATCAGGATGTTGTCCAC
>scaffold00001_3_4_183_R
ATCAACTGCCAGAACTCGTCGGTCCTCATG
>scaffold00001_4_5_568_F
TACGTCGAGGAACTGGGCGACTTCAAGACC
>scaffold00001_4_5_838_R
CATGTCGTCGATCTGCACGTTGACCAG
>scaffold00001_5_6_1090_F
GTTGAAGAAGCTGACCAGTCCGACGAGTAC
>scaffold00001_5_6_374_R
CGGGTCTTCTTCGAGATGGACATCTACTG
>scaffold00001_6_7_939_F
ATCAGCGGTTACGTCTGGTGGTGAC
>scaffold00001_6_7_870_R
CGATCTCCACGTTTGACAGGATGCTCTG
>scaffold00001_7_8_818_F
GAACTGGAGTTGATGGAGCAGCGCGAAAC
>scaffold00001_7_8_545_R
ATCAGTTTGATCGGCGAGACGTGCGAGACC
>scaffold00001_8_9_346_F
CTCGTGCTGATCGTCGAGTACGCCATCTTC
>scaffold00001_8_9_358_R
ATGGACATGGTCCACCTGGTCATCG
>scaffold00001_9_10_1135_F
AGACCGGATTGAGTGCTTCCTCTGCTGGTG
>scaffold00001_9_10_1408_R
CGACCAATTTCCGACGAGGTGACATTC
>scaffold00001_10_11_533_F
TTCGCCACCACCGGTCTAGGATGAGAGATC
>scaffold00001_10_11_191_R
ATTCCTCGCGCAGTTGACCCAGTTC
>scaffold00001_11_12_354_F
AAGGTCAGGATGGCGGTGAACACCTTGGTC
>scaffold00001_11_12_345_R
ATGTTGTCGCTGACCTTCGCGATACGGGAG
>scaffold00001_12_13_1313_F
TTTCAGGCTTTGAGGATCGAGGTAAACAGG
>scaffold00001_12_13_315_R
TCGCATCTTCTCAGCCAGTTCCGAG
>scaffold00001_13_14_961_F
GTCATCGGACAGCCGATTCTTCGACGACTG
>scaffold00001_13_14_1221_R
AGTCGTCGAAGCGCAGAAGGTACATGTAGG
>scaffold00001_14_15_1407_F
CTTGTTTCGATAGCATTACCGCCCAGTAAC
>scaffold00001_14_15_533_R
GTACTCGTCGAAGGTGGACACGTTCACTC
>scaffold00001_15_16_126613_F
Can't find proper primer
>scaffold00001_15_16_569_R
CTTCTACCACGACACACACACCGATCC
>scaffold00001_16_17_1785_F
CTCGAAGACGTCAACAACCACACCAATCTG
>scaffold00001_16_17_478_R
CTCGTAACTCTCCATCGGTTCTCCAGTAAC
>scaffold00001_17_18_225_F
GATCGTGACGAACGTGAAGGTCCAC
>scaffold00001_17_18_408_R
CAGATCCGGTACAGCTCCTTGTATTCCAG
>scaffold00001_18_19_320_F
CAGATAGATGGTGAAGTCAGGACGACGG
>scaffold00001_18_19_177_R
ATGAACTTTCCACCGATGCCGGTCGTCTTC
>scaffold00001_19_20_313_F
GTGTCGTTGTAGGCGATGAGGAGTTGGTG
>scaffold00001_19_20_645_R
CTGTCTACAACATCCCCGTCGCACTCCAAC
>scaffold00001_20_21_312_F
TGTCGTTGTAGGCGACGAGGAGCTGATCAC
>scaffold00001_20_21_428_R
TCGGACATGTCTTCGACCTGAGTGC
>scaffold00001_21_22_243_F
AAGGACGGACGTGGACATCGATCAGCAATC
>scaffold00001_21_22_1379_R
AGGACTCCTGTACGTCGATCACGACGAGTG
>scaffold00001_22_23_238_F
GTTTCACTCATCGGTGTACCCTCCCGATC
>scaffold00001_22_23_244_R
GAAGTTCGTCATCGAGAACGTCTCCGTGC
>scaffold00001_23_24_410_F
ACTGACCACGGTGCAGCTCTACTTCAC
>scaffold00001_23_24_1184_R
AGCGTGTAGTCGGCGGTATACACCTTC
>scaffold00001_24_25_444_F
ATTCGACGGGCAGGACGTCAACAAG
>scaffold00001_24_25_458_R
TAGGTGTGCTCCAGGTAGGTGAGGAAC
>scaffold00001_25_26_203_F
GTCGAGGGGATCAACACAAGTGGAATCAAG
>scaffold00001_25_26_184_R
GTACCGTCGACAAGCAACTCCTGATCGTC
>scaffold00001_26_27_369_F
ATGAGCGAGCAGGTCTTCCTCCTAC
>scaffold00001_26_27_706_R
GAACACGCCGTAGTTGTTCAGCGTGAAG
>scaffold00001_27_28_280_F
TGACGTGAGGGGACTACGGATGTAC
>scaffold00001_27_28_260_R
ACTTCGACAGGTAGGCCTTGCAGAG
>scaffold00001_28_29_209_F
TCTACGGTGGGTTGCACTGGTTTAGAGAAG
>scaffold00001_28_29_357_R
TACAGCTGAAGGATCTTCTCGTCGAGGACC
>scaffold00001_29_30_1643_F
CCGATCCAGGCGAAGATCAGATTGGTC
>scaffold00001_29_30_767_R
ATCGACATAGCGCTCCAACAGATCGGTGAC
>scaffold00001_30_31_237_F
CACCACACGTTGTACGTCTTCGTGCTCTAC
>scaffold00001_30_31_286_R
GACATCCCCCAAGTCTTTCCTCTCATGCTC
>scaffold00001_31_32_566_F
AAACGAGCCGTACAACGGGCAACTG
>scaffold00001_31_32_169_R
ATCTTCCTGACCTCGTCGGTGTGGTACTTC
>scaffold00001_32_33_266_F
AACATTGACAGGGTGGCACGCAGTTCG
>scaffold00001_32_33_169_R
TTGTCAATGTAGCGTTGACGGGTGCCTGAC
>scaffold00001_33_34_795_F
GTCGACGACGAGAATCCGGTTCATG
>scaffold00001_33_34_365_R
GTTCCCTGCTGCTGAACTGGTTCTTCAC
>scaffold00001_34_35_1071_F
CAGTACGTCATCTTCGACCGGACCATTTG
>scaffold00001_34_35_2096_R
AGTCGTCGTCGTCGAACATCGTGAC
>scaffold00001_35_36_685_F
GTCGTTCAGGTTCAGGTAGAACTCGGTGAC
>scaffold00001_35_36_0_R
Can't find proper primer
>scaffold00001_36_37_1073_F
GATCGGTTTGCCGCGGTCGTTGTATTCGAC
>scaffold00001_36_37_740_R
ATACGGCTTCGTCGAGAACCTGACC
>scaffold00001_37_38_498_F
TTCGACGAACTGTGGCAACGCTTCTCC
>scaffold00001_37_38_562_R
AGGACGTCATGCACGGTCAACACAC
>scaffold00001_38_39_612_F
AACATGTCGTTGAGCGCGGCCAGTTTG
>scaffold00001_38_39_672_R
CTCTTCGACGAGATCATCCAGGTGCTG
>scaffold00001_39_40_196_F
CACAATTTCATCGATCAACACAAACGCGAG
>scaffold00001_39_40_425_R
ACCTGCAACTGACTCGGCTGGCTATAGCTG
>scaffold00001_40_41_522_F
GACTACAAGCCGGAGAACGTGCTCATCTC
>scaffold00001_40_41_660_R
GTGCAGAAGGGATAGAGGGTGACGTAC
>scaffold00001_41_42_205_F
ACCGAAGCAGTCGAAGACGGTACAC
>scaffold00001_41_42_339_R
GTCTTCTTCTGGCCCCGCAACGAGATCATG
>scaffold00001_42_43_389_F
TAAAATCTGTGATCTGGGCTAACCTTGGGG
>scaffold00001_42_43_223_R
GTGATCGGTGTGTTCGTGGCTGACCAGTAC
>scaffold00001_43_44_440_F
CTCGGAGGAAAACGGTGAACAGTCAGCAAC
>scaffold00001_43_44_563_R
TGAACGCCTCCACCCAGCATTTGATCACAG
>scaffold00001_44_45_93801_F
Can't find proper primer
>scaffold00001_44_45_485_R
GTGTTGCGATCGGATTCGTAGCTGAC
>scaffold00001_45_46_401_F
GGATCAGCTCCGGATAGTTGAAGTCGTC
>scaffold00001_45_46_423_R
ACAACGAGGACTTCGACAACGGCTTCAAGG
>scaffold00001_46_47_370_F
GTAAACTCCTGATCTCAGCCGCTCTCGTG
>scaffold00001_46_47_845_R
CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG
>scaffold00001_47_48_1970_F
GACGACTACCTGCGTAAACCCTTCGACCTG
>scaffold00001_47_48_1044_R
CACCAATCTGCTTGTGGGTCTGGTGCTGAC
>scaffold00001_48_49_1639_F
GATGTTCATCGACCTGGAGAACGTGC
>scaffold00001_48_49_244_R
CATCTCTCCCGTCAGCTCATCGTTCGAAG
>scaffold00001_49_50_1297_F
CAGGGTTTCCTGAGCCACCTTGAAC
>scaffold00001_49_50_822_R
CTGTTCTCCCAGGACCCGATCTTCAACTC
>scaffold00001_50_51_227_F
CGTGCCATGTTCTGAACGAGAACGATC
>scaffold00001_50_51_860_R
GTGACCAGCGAGTGAATCTTGTCGCGTTG
>scaffold00001_51_52_1410_F
GATGTCCACGTCGTACGCCATGCTGTTGTC
>scaffold00001_51_52_509_R
CCTGATTCACCTGATTCGTCCACCAGTTC
>scaffold00001_52_53_361_F
GTCATCCACACATGGCATTCTGTGACC
>scaffold00001_52_53_353_R
AGGATGGCGTAATCCGCGATGATGG
>scaffold00001_53_54_414_F
TTCTGGAAGACCATCTGCAGACCGATCTCG
>scaffold00001_53_54_823_R
TACGCGAAGGGACGTTAGAGATGGCAC
>scaffold00001_54_55_1250_F
CTGGAACCGGATGATGTTGGAGTCCTC
>scaffold00001_54_55_175_R
TACCGAGGAACTGATCGACCTCTCC
>scaffold00001_55_56_239_F
CACCTGACCTCTGACAATGAACGCCGAAAG
>scaffold00001_55_56_206_R
GATTGGCTTCTATACAAACTAAGGTGGCGC
>scaffold00001_56_57_412_F
GTACTCACGATGGTCCTTCTCCTCGTTG
>scaffold00001_56_57_184_R
CAAACCGGTGGTCGGGCTGATCATGTATTC
>scaffold00001_57_58_1204_F
TCATGCTGTCCGTATCCATTTAGGACCTTC
>scaffold00001_57_58_939_R
TTCTACCGTGCCTACTTCGAGACCGAC
>scaffold00001_58_59_788_F
CAAAGCAAATCGGAGGGGACATGACG
>scaffold00001_58_59_229_R
ACACGTTCGTCCGCAACAACGGATG
>scaffold00001_59_60_1979_F
GTGACCAGCCAGGTCTGTTCGAATCTCAC
>scaffold00001_59_60_625_R
GAGATCATGTACGCCGACTTCAGCTTCGTG
>scaffold00001_60_61_755_F
GTACGGTTTCTGACCAACACCGACTCGAAG
>scaffold00001_60_61_647_R
CCAACATCACCATCACGAACCTCGG
>scaffold00001_61_62_285_F
TCAGGTTGAGATCGACCATCGCGAG
>scaffold00001_61_62_944_R
TTGCACCATCAGCTCAGCATCGTCTTCGAC
>scaffold00001_62_63_898_F
CTGATGATCAAGGTGTTGCGGCTGTC
>scaffold00001_62_63_367_R
GTTGTAGCCGCCGAAGTCGTTCATGCTGAC
>scaffold00001_63_64_711_F
ACGTAGTTCCCGACCGTAGAGCAGTTC
>scaffold00001_63_64_1286_R
CGATAGGTGAGACTCGATGCATGGTTAGTG
>scaffold00001_64_65_324_F
CTGGTGTGCTTTTCGGTTCGGGTAC
>scaffold00001_64_65_297_R
AACGCCCACGTCATCCTCGAAGAAC
>scaffold00001_65_66_1986_F
GATCAGTCCGGCGATCGACGAGTAGAGAAC
>scaffold00001_65_66_1292_R
GTTGCTGCTGGAAGTGGCATGGGAAAC
>scaffold00001_66_67_428_F
CTTCGTCACTGGCTGTGTCAAGGTCAAC
>scaffold00001_66_67_346_R
ACTGTTCCTGCTGTACTCCTCGGTG
>scaffold00001_67_68_5122_F
Can't find proper primer
>scaffold00001_67_68_0_R
Can't find proper primer
>scaffold00001_68_69_5187_F
Can't find proper primer
>scaffold00001_68_69_1373_R
CTCCTGGTGGGCTCGGTGAAAACCAACATC
>scaffold00001_69_70_254_F
CTTCGACATGGAGGCAGTGCTTGAGAACTC
>scaffold00001_69_70_318_R
AGTGAGGAGGAACAGTTCTACAATGGTGTG
>scaffold00001_70_71_240_F
CATTCGGCGTGGTAAAGACCCACTGGTAC
>scaffold00001_70_71_233_R
ATCGATGACCCTCCAAGACAGACCTCATTG
>scaffold00001_71_72_349_F
AACCCACGCATCCCACTCAACGAGCTGAAC
>scaffold00001_71_72_0_R
Can't find proper primer
>scaffold00001_72_73_2964_F
Can't find proper primer
>scaffold00001_72_73_0_R
Can't find proper primer
>scaffold00001_73_74_2968_F
Can't find proper primer
>scaffold00001_73_74_0_R
Can't find proper primer
>scaffold00001_74_75_356_F
GTGTTCACCGGCGTGATGTACAACGACTAC
>scaffold00001_74_75_0_R
Can't find proper primer
>scaffold00001_75_76_788_F
GTCGACGTTTCATCACGTCACTGGGTG
>scaffold00001_75_76_0_R
Can't find proper primer
>scaffold00001_76_77_1854_F
GTGATCAAGGTGATCGAAGCCCTGC
>scaffold00001_76_77_535_R
ATCTACAACCACCTGGGCGGCAAGGAGAAC
>scaffold00001_77_78_624_F
AATCTCATGGGGTGCCTTCGTCGATCGGTGAG
>scaffold00001_77_78_349_R
CTCGGCAACCGACTGTGGAACAACATC
>scaffold00001_78_79_195_F
GTAGTTGCACGAGACGGCCATTACCTTG
>scaffold00001_78_79_276_R
GAAATGCCGTCACTCTCCGTAGATGTCC
>scaffold00001_79_80_301_F
TACGTTTGGCTCAACACCCCACGCGAGATG
>scaffold00001_79_80_212_R
GTCGCTGATCATGGTTCGGTAGACGATGTC
>scaffold00001_80_81_426_F
CACTGCCCAAACCGATCTATCCGACGTTC
>scaffold00001_80_81_202_R
GATCCAAGCTGTCTTAACGAGGAGTTGCC
>scaffold00001_81_82_751_F
CAAATCCGGGCACCTCGAACTCAAG
>scaffold00001_81_82_233_R
AACACCACCACCCCCGAGTAGCAGACAACAAC
>scaffold00001_82_83_253_F
CAAGGTCATCGTCAGCGGTGGATTG
>scaffold00001_82_83_302_R
AGATCCGGATACTGCGCTGGGAAAC
>scaffold00001_83_84_243_F
GTGGGGCAAGTGCTGGAGTTTGATTCAC
>scaffold00001_83_84_182_R
GTGGACGGACCAGTCGTGATGTTCGATTC
>scaffold00001_84_85_414_F
GTTTACCCTCGATCCGGCAGATGAAGC
>scaffold00001_84_85_726_R
GACCCGTCCAACACCTTCGATCAGAGCTTC
>scaffold00001_85_86_202_F
GTTTAGGAAGTGTGGACACGGCCGGAAGTC
>scaffold00001_85_86_191_R
CCGAAGTGTGCAAACGTTGTCTTTACGTC
>scaffold00001_86_87_218_F
TCAACAACCCAGTGAACACCGAGGACGTAC
>scaffold00001_86_87_245_R
CATTGAGAAAGCGATGTACCGCCACGAGAC
>scaffold00001_87_88_204_F
GAAGTGATCACGCGATCCACGGGATTCCAC
>scaffold00001_87_88_267_R
AGCTTCCAGACCCTGGTCAACAGTC
>scaffold00001_88_89_1973_F
AGTAGCCGATGACCCCGTTGAGTTG
>scaffold00001_88_89_463_R
CAGGTGTTCAACCTCGCCCAGACCTTCTAC
>scaffold00001_89_90_613_F
ATGACCTTCGTGAAACTGACCTTCTCGCTG
>scaffold00001_89_90_528_R
GACAAGGACTCCGCCAAGAAGGACGAATC

primer part 2 - scaffold00003, scaffold00004

>scaffold00003_1_2_276_F
GACGTGTACATCTCGAACATCTCGCGG
>scaffold00003_1_2_387_R
AAAACGGGCGTTCACCGCATGGTAC
>scaffold00003_2_3_232_F
CAAGGGAGAGTTCCGTTACGACCACAG
>scaffold00003_2_3_460_R
TTGATCAGCCGGCACAGCAACAAGGTGGTG
>scaffold00003_3_4_218_F
ACAAGCTCATTGCCTACTAGATCGACTTCG
>scaffold00003_3_4_1054_R
GCATCGTCCAACGGTAGCCTTCTTTC
>scaffold00003_4_5_574_F
ATCGACCTGGTGCACGCGAACAACTCCAAG
>scaffold00003_4_5_289_R
GAAGAAGGCGTTGGAGTGCCACAGTTG
>scaffold00003_5_6_195_F
ATCTGTTGCCGAATTGGCCTGTACTAGATC
>scaffold00003_5_6_263_R
ATGGCATCCCTCAACCACTACCACC
>scaffold00003_6_7_918_F
ATGCTCAGTCCGGGAGTACAGCTCGGTAAC
>scaffold00003_6_7_245_R
GCATGCTGTTGTTGATGCTGTCCTTCAG
>scaffold00003_7_8_365_F
ATCCGACGAGTACGACATCCACCTG
>scaffold00003_7_8_601_R
CAGACCAACTACGCCACAAGCAAGG
>scaffold00003_8_9_472_F
CTGTTCCAGACCTTCCTCACCGTGCTTGAC
>scaffold00003_8_9_218_R
CTTATGCCCTGACAGAAGGTGCAAGATCAG
>scaffold00003_9_10_376_F
CATGCACAAGCAGGACCTCATCGAG
>scaffold00003_9_10_212_R
TTTCCGTTGAGCCGCCACTCGTACTTGTAG
>scaffold00003_10_11_1436_F
TGGTCCTGCCGTACTTCCTGATCACCACTG
>scaffold00003_10_11_232_R
GATGGGACAGACTTCGTACACCGGTTG
>scaffold00003_11_12_266_F
CTGTATTGATGATCCACTCGCTGCCGCTC
>scaffold00003_11_12_1184_R
TACAGTGATCGGCCGTTGTTGGAGTGTGTG
>scaffold00003_12_13_2070_F
CTCCAGCAGGGTTTCATCGATTCGGTAG
>scaffold00003_12_13_324_R
CTGGACCAGTTCACCCTCCAGATCATG
>scaffold00003_13_14_463_F
CTGGTGTCACACAACGAAAAGGACCTGAC
>scaffold00003_13_14_304_R
GAAGAAGTCGTCCTTCTCCTTGACCGACAG
>scaffold00003_14_15_137305_F
Can't find proper primer
>scaffold00003_14_15_518_R
AATACCGCTACGGTGCTGGAACCGATGTAC
>scaffold00003_15_16_227_F
GTTTCTGGACAAGTACGGTTTCGGGACC
>scaffold00003_15_16_170_R
CTTGAGGCGCTCACTGATGCACTTAAC
>scaffold00003_16_17_319_F
TCACGTGATCTCCTGATAGGTAGACAGACG
>scaffold00003_16_17_503_R
TACCTGTGGCCCCAAAGCTACTACCTG
>scaffold00003_17_18_314_F
TCTGAACGAGACGGAATGGGAGATCCTCAC
>scaffold00003_17_18_222_R
GACATCACCATCCAGGCAGAGAACATGCTG
>scaffold00003_18_19_746_F
GTTGCTTCGAATCTGGTGACGGGAG
>scaffold00003_18_19_471_R
GTATACCGCAACATCGGCGAAATGAAGTC
>scaffold00003_19_20_655_F
GAAGGAACGGCAACTTTCGGTCACGGTAC
>scaffold00003_19_20_485_R
CAAGGAGACCGTCTACAAGATCTGGCATC
>scaffold00003_20_21_294_F
ATCAGCCCCGAGCAATTGACCGACCTGAAC
>scaffold00003_20_21_200_R
GAAGATCGGCAGCTCCATCGTCTCATC
>scaffold00003_21_22_1034_F
GAAGTAACCGGTGGTGTTTTCGGCCAG
>scaffold00003_21_22_354_R
ATCTCGCCGGTTACAAGCAGATCCTC
>scaffold00003_22_23_1299_F
TTCAGATGGTTAAGGACAGCTTCATGAGCG
>scaffold00003_22_23_368_R
ACGTGATCGGTGGGAACTTCGACTC
>scaffold00003_23_24_235_F
CCCAGGGAATATAACGATGGTTCAGGTCAG
>scaffold00003_23_24_1163_R
GAACTGCTGGTTCATCGAATGGTCGTTGAC
>scaffold00003_24_25_268_F
CTCGAAGTCCCGGTCAAGAAGATCCTCAC
>scaffold00003_24_25_666_R
TATCGTTCGCGTCGATGGCCGACAACATTG
>scaffold00003_25_26_360_F
GAAAGGCTGACCATCTACCCGGAGTAC
>scaffold00003_25_26_636_R
TGATCCACGTGTCCGTACATCATCGTG
>scaffold00003_26_27_1270_F
GAAATCAAGATCCTGAACCAGTCCCGGAAG
>scaffold00003_26_27_244_R
CCCGATTCCTTGTCCTGGAACAGATAGAAG
>scaffold00003_27_28_833_F
GTACTCGCCGACTTTCCCGTTCTCATC
>scaffold00003_27_28_474_R
GTACACCTCGTGCTCGGGACACAAATGGTC
>scaffold00003_28_29_210_F
GTACACGTGCCGTTCCCGTTTCTGGTGAAC
>scaffold00003_28_29_173_R
ACGGCGTCACGAACAACACCTACTG
>scaffold00003_29_30_703_F
TCAGCGATTCAGAGGTCCCGATCAGAGGTC
>scaffold00003_29_30_1341_R
TACCTGCATACCGTACGTGGAGTCG
>scaffold00003_30_31_256_F
TTCCGGGGATGAGTAGCCGACCAATTGTCC
>scaffold00003_30_31_1239_R
GATCACCGGTTGGTAGACCAGATCCAG
>scaffold00003_31_32_351_F
CACAAATTGAACTTGAGCTGGGATGTCAGG
>scaffold00003_31_32_565_R
CAGGATGACAGTGTGAGTCAGCCATGGAC
>scaffold00003_32_33_376_F
AGATCGACGAGCTCAACGTCTATCCG
>scaffold00003_32_33_1069_R
GTCATTCGACACCCACCAGCAACGGATAG
>scaffold00003_33_34_1969_F
GTCCTCAAGCATCGCAAGCGCAAGGAAAAG
>scaffold00003_33_34_485_R
CTCCAACTGCTCGGAGAGGAACTTGTAAC
>scaffold00003_34_35_491_F
GAACGAGACCAGGAGACATAGTGGCGAGTC
>scaffold00003_34_35_222_R
ATCTCCGCAGTCATATCGGTACGGG
>scaffold00003_35_36_707_F
AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
>scaffold00003_35_36_243_R
CAGGTTGAACATCTCCTGGACCAGCGAAC
>scaffold00003_36_37_355_F
CTGCTCTTCCTCGGCATGCTCATGTTC
>scaffold00003_36_37_430_R
ATAGCCCGCCCACTGCATGACAAAC
>scaffold00003_37_38_542_F
CGACTGGGAGTACTACATCGGTAACACC
>scaffold00003_37_38_565_R
GCACTGCGTGAACTCCCTTTCCATC
>scaffold00003_38_39_791_F
AACTGTCGACGAAGAACCGCGTCATGAGAG
>scaffold00003_38_39_613_R
GTACGAGCAGTTCACCAGTTGCGTG
>scaffold00003_39_40_351_F
CACTTGTCTTCTCCTCCGGATACCTGG
>scaffold00003_39_40_333_R
CTGATCCGTCATGGCGCACATTCCACGATG
>scaffold00003_40_41_416_F
GTGGTAGGTCTGTGCCTGGTGGTTATG
>scaffold00003_40_41_370_R
AAGTAGTGCGGCTCCTGCCATCTTCGTTTG
>scaffold00003_41_42_419_F
GTAGGTCTGTGCCTGGTGCTGGTGGTTATG
>scaffold00003_41_42_166_R
GTACCGCCAACACGACGACTCATTC
>scaffold00003_42_43_317_F
GTTCCTGTCCTCGTGCAGCACAATTCGATC
>scaffold00003_42_43_298_R
ACCGGGGATCATCGTGGTCTTGTAC
>scaffold00003_43_44_737_F
ATCGTTCGCAGCGGCATCGATTCAC
>scaffold00003_43_44_992_R
TGAAGCTGACCAAGCTGGTGGTCTC
>scaffold00003_44_45_1117_F
CTCTGTGAGTACGTGGAACAGGCGATG
>scaffold00003_44_45_224_R
AAGATCACCACGGCGTCGACACAGCTCAAC
>scaffold00003_45_46_422_F
GTGATGCCCTCGGATCCCAGTTCTTCGTAC
>scaffold00003_45_46_399_R
AACTCGGTCAAGCTCGAGTTCCTGGTG
>scaffold00003_46_47_258_F
GACCTGCTGAGTGGATCATCAACCTATCG
>scaffold00003_46_47_510_R
GTTGTAACCCATGGCTGACGAAGTAGGG
>scaffold00003_47_48_427_F
CATCACGGCGAACAACAACCAGACCTAC
>scaffold00003_47_48_271_R
GAACAACGGCATGAGGTCCTTGAACATGAC
>scaffold00003_48_49_532_F
GAAGGGTCAACAGTGAGATCGCCTGGTTC
>scaffold00003_48_49_741_R
TGTAGCTGCCCTTCTCCTCCTGTTC
>scaffold00003_49_50_208_F
GTGGTGTGCATCGTGCAGATCCCGTTCTAC
>scaffold00003_49_50_975_R
AACCCGGAGCCGAAGAGTTCGAGAAAC
>scaffold00003_50_51_1445_F
TGGCGTACCTGGAGAAGGTGGTTTC
>scaffold00003_50_51_439_R
CAATGGCACCCTCACATATGGCAGATAATTG
>scaffold00004_1_2_381_F
GAACACGTCACAACCGTGCTTCCAG
>scaffold00004_1_2_1879_R
ATCGTCTGGGTCAACACCTGGTTCCTG
>scaffold00004_2_3_250_F
GTTGAACCGTCTGAACGACCGAGGAAG
>scaffold00004_2_3_411_R
TGTTGTCGGGGTAGGTGGGTAAACG
>scaffold00004_3_4_333_F
TGACTTCTCCGACCAGATCGCACACCTGTC
>scaffold00004_3_4_675_R
GAGCTTCTCCTTGTGCTGGGTCAAC
>scaffold00004_4_5_297_F
GGGAGGTTGTAGATCACGAACTGGATGTAG
>scaffold00004_4_5_180_R
CAGAACGAGTCCTACAACGGGAACCTTTC
>scaffold00004_5_6_411_F
GAGATCATGTCGCTGGGCACCAATC
>scaffold00004_5_6_469_R
AGCAGAGCACCCGTCTGATGTACAG
>scaffold00004_6_7_257_F
CGCGTTGAAAAACCGGGCGATATCC
>scaffold00004_6_7_545_R
GTCTTCGTGGTCAACAAGGCCGATC
>scaffold00004_7_8_584_F
TGATCAACGAGGCGTGCAACGGTAG
>scaffold00004_7_8_419_R
ATGATCGACACCGTTACGGCGAGATGG
>scaffold00004_8_9_447_F
GAACCTGCTGTTCATGGTCGGTCTG
>scaffold00004_8_9_234_R
ACAGTGGTCTCCGTCTACAGTCCAC
>scaffold00004_9_10_1726_F
TAGAGGGTCAACGGTTCGTCACCAG
>scaffold00004_9_10_313_R
GTCAAGGATGACTGCGTGACTGGCTAC
>scaffold00004_10_11_783_F
AGAGCAGTTCGAGGATGTTGGGGTC
>scaffold00004_10_11_256_R
GCCGACTTCTACGTACTGCTGTCCATG
>scaffold00004_11_12_1607_F
TACCGACAAGTGAGAGGACGACGTG
>scaffold00004_11_12_704_R
GACCTGATCGCGGTGATTGGTTGAC
>scaffold00004_12_13_750_F
TCGATGGACATGCTCTCCTCCGAGATCGTC
>scaffold00004_12_13_895_R
TATGCGAGCGTCCGAAAAAGATCGACCAGC
>scaffold00004_13_14_550_F
GAGCACATCAAGATCACTGACAATGGGATC
>scaffold00004_13_14_243_R
AACACTCCGAAATCCGCTGGCTCTC
>scaffold00004_14_15_556_F
CTGGTGGGACTCGTTGAGCATCCAATG
>scaffold00004_14_15_220_R
CCGAGGGAAAGAACCGGACATTGTG
>scaffold00004_15_16_1515_F
CTTCACCGGCCTGTTCTTCGTCATCAC
>scaffold00004_15_16_167_R
CAGTACCTGTGGCGTCACACGCGATAAGAG
>scaffold00004_16_17_236_F
GGTTCGACACCAGGATTTCGAGGTCAC
>scaffold00004_16_17_467_R
GATCTGGTTCCACATCTGTCCCTGGTG
>scaffold00004_17_18_420_F
CCTGAGGCACAAGTCAGGGTATCGATC
>scaffold00004_17_18_720_R
CCAACATCAAGAGCCACCACAACGTC
>scaffold00004_18_19_226_F
GTGTGATCGACACGGTTACGTCGAAG
>scaffold00004_18_19_492_R
TTAACAGGGGGCCCTTCCTTCAACACGGTC
>scaffold00004_19_20_273_F
GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC
>scaffold00004_19_20_878_R
GACGAGCAACTGGACTCGCTGAACAAG
>scaffold00004_20_21_2075_F
AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
>scaffold00004_20_21_521_R
TGAAGATCACCCAGGATCTCAAGGAGTACG
>scaffold00004_21_22_386_F
GAAGACTTCGCCACCGAATACCTCAGC
>scaffold00004_21_22_182_R
TTCGACGTACACACCACCCTCGCCGTAATC
>scaffold00004_22_23_594_F
AATTCAGCGGACAGCGGTACGAGGGTGTAG
>scaffold00004_22_23_1225_R
AACAGGACCTGCTCGACAACAAGATCAAG
>scaffold00004_23_24_720_F
TCCACCGGAACATGGGTGATACTCGAC
>scaffold00004_23_24_552_R
GTTGCCTTTGCACAGGTAGATCCCG
>scaffold00004_24_25_667_F
CAAGCCATGTCGAAGCAGCACTACTC
>scaffold00004_24_25_322_R
AAGCACCTGCTGTTCACAAGCGAACTGATC
>scaffold00004_25_26_542_F
GCAGATCGACGAGTACCTGGAGTTCTAC
>scaffold00004_25_26_247_R
AGGAGTCCGACCATGAAGATCAGGAAGATC
>scaffold00004_26_27_255_F
GATCGTTCGACTACCCACAATCCGCAGATC
>scaffold00004_26_27_671_R
GGTAGAGCAGCCATTCCAGTTCCTGTG
>scaffold00004_27_28_577_F
CTGTTCACCGAGGAGGGCGAGTTCTTCTTC
>scaffold00004_27_28_485_R
GAAAACGGGGTGTTCCAGCTTCATCGCTTC
>scaffold00004_28_29_1409_F
AACCGACACACCTGCAGTTGCCGTACCAAC
>scaffold00004_28_29_207_R
TACTCCGGGATAAGCAAGGCTGAGCTG
>scaffold00004_29_30_278_F
CATTTGTGACACCTGTCCAGCAGTACG
>scaffold00004_29_30_264_R
TCTGGTGAGCCGACAGGTATTCCTC
>scaffold00004_30_31_444_F
ACCTTCTTGCTCTTACGGCCCACCTTG
>scaffold00004_30_31_739_R
GAGTACGTCAAGTTCATTTTCGCCACCAC