Primer Verrucosispora gap

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(→scf3)
(→primer part 1 - scaffold00001)
Line 55: Line 55:
*By Using Primer3
*By Using Primer3
*"Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer).
*"Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer).
-
===primer part 1 - scaffold00001===
+
===primer intergenic===
-
>scaffold00001_1_2_1105_F
+
-
CTCAGGTCAGGCAGGCGTGATGATCTCTTC
+
-
>scaffold00001_1_2_529_R
+
-
TACTAACCTCGGGTCGGCGTGAGAGGAGATTG
+
  >scaffold00001_2_3_751_F
  >scaffold00001_2_3_751_F
  CAGAACTGGAGAACGTCCATGCTCGAGAAC
  CAGAACTGGAGAACGTCCATGCTCGAGAAC
Line 72: Line 68:
  >scaffold00001_4_5_838_R
  >scaffold00001_4_5_838_R
  CATGTCGTCGATCTGCACGTTGACCAG
  CATGTCGTCGATCTGCACGTTGACCAG
-
>scaffold00001_5_6_1090_F
 
-
GTTGAAGAAGCTGACCAGTCCGACGAGTAC
 
-
>scaffold00001_5_6_374_R
 
-
CGGGTCTTCTTCGAGATGGACATCTACTG
 
-
>scaffold00001_6_7_939_F
 
-
ATCAGCGGTTACGTCTGGTGGTGAC
 
-
>scaffold00001_6_7_870_R
 
-
CGATCTCCACGTTTGACAGGATGCTCTG
 
-
>scaffold00001_7_8_818_F
 
-
GAACTGGAGTTGATGGAGCAGCGCGAAAC
 
-
>scaffold00001_7_8_545_R
 
-
ATCAGTTTGATCGGCGAGACGTGCGAGACC
 
-
>scaffold00001_8_9_346_F
 
-
CTCGTGCTGATCGTCGAGTACGCCATCTTC
 
-
>scaffold00001_8_9_358_R
 
-
ATGGACATGGTCCACCTGGTCATCG
 
  >scaffold00001_9_10_1135_F
  >scaffold00001_9_10_1135_F
  AGACCGGATTGAGTGCTTCCTCTGCTGGTG
  AGACCGGATTGAGTGCTTCCTCTGCTGGTG
  >scaffold00001_9_10_1408_R
  >scaffold00001_9_10_1408_R
  CGACCAATTTCCGACGAGGTGACATTC
  CGACCAATTTCCGACGAGGTGACATTC
-
>scaffold00001_10_11_533_F
 
-
TTCGCCACCACCGGTCTAGGATGAGAGATC
 
-
>scaffold00001_10_11_191_R
 
-
ATTCCTCGCGCAGTTGACCCAGTTC
 
  >scaffold00001_11_12_354_F
  >scaffold00001_11_12_354_F
  AAGGTCAGGATGGCGGTGAACACCTTGGTC
  AAGGTCAGGATGGCGGTGAACACCTTGGTC
Line 104: Line 80:
  >scaffold00001_12_13_315_R
  >scaffold00001_12_13_315_R
  TCGCATCTTCTCAGCCAGTTCCGAG
  TCGCATCTTCTCAGCCAGTTCCGAG
-
>scaffold00001_13_14_961_F
 
-
GTCATCGGACAGCCGATTCTTCGACGACTG
 
-
>scaffold00001_13_14_1221_R
 
-
AGTCGTCGAAGCGCAGAAGGTACATGTAGG
 
-
>scaffold00001_14_15_1407_F
 
-
CTTGTTTCGATAGCATTACCGCCCAGTAAC
 
-
>scaffold00001_14_15_533_R
 
-
GTACTCGTCGAAGGTGGACACGTTCACTC
 
  >scaffold00001_15_16_126613_F
  >scaffold00001_15_16_126613_F
  Can't find proper primer
  Can't find proper primer
  >scaffold00001_15_16_569_R
  >scaffold00001_15_16_569_R
  CTTCTACCACGACACACACACCGATCC
  CTTCTACCACGACACACACACCGATCC
-
>scaffold00001_16_17_1785_F
 
-
CTCGAAGACGTCAACAACCACACCAATCTG
 
-
>scaffold00001_16_17_478_R
 
-
CTCGTAACTCTCCATCGGTTCTCCAGTAAC
 
  >scaffold00001_17_18_225_F
  >scaffold00001_17_18_225_F
  GATCGTGACGAACGTGAAGGTCCAC
  GATCGTGACGAACGTGAAGGTCCAC
Line 128: Line 92:
  >scaffold00001_18_19_177_R
  >scaffold00001_18_19_177_R
  ATGAACTTTCCACCGATGCCGGTCGTCTTC
  ATGAACTTTCCACCGATGCCGGTCGTCTTC
-
>scaffold00001_19_20_313_F
 
-
GTGTCGTTGTAGGCGATGAGGAGTTGGTG
 
-
>scaffold00001_19_20_645_R
 
-
CTGTCTACAACATCCCCGTCGCACTCCAAC
 
-
>scaffold00001_20_21_312_F
 
-
TGTCGTTGTAGGCGACGAGGAGCTGATCAC
 
-
>scaffold00001_20_21_428_R
 
-
TCGGACATGTCTTCGACCTGAGTGC
 
  >scaffold00001_21_22_243_F
  >scaffold00001_21_22_243_F
  AAGGACGGACGTGGACATCGATCAGCAATC
  AAGGACGGACGTGGACATCGATCAGCAATC
  >scaffold00001_21_22_1379_R
  >scaffold00001_21_22_1379_R
  AGGACTCCTGTACGTCGATCACGACGAGTG
  AGGACTCCTGTACGTCGATCACGACGAGTG
-
>scaffold00001_22_23_238_F
 
-
GTTTCACTCATCGGTGTACCCTCCCGATC
 
-
>scaffold00001_22_23_244_R
 
-
GAAGTTCGTCATCGAGAACGTCTCCGTGC
 
  >scaffold00001_23_24_410_F
  >scaffold00001_23_24_410_F
  ACTGACCACGGTGCAGCTCTACTTCAC
  ACTGACCACGGTGCAGCTCTACTTCAC
  >scaffold00001_23_24_1184_R
  >scaffold00001_23_24_1184_R
  AGCGTGTAGTCGGCGGTATACACCTTC
  AGCGTGTAGTCGGCGGTATACACCTTC
-
>scaffold00001_24_25_444_F
 
-
ATTCGACGGGCAGGACGTCAACAAG
 
-
>scaffold00001_24_25_458_R
 
-
TAGGTGTGCTCCAGGTAGGTGAGGAAC
 
  >scaffold00001_25_26_203_F
  >scaffold00001_25_26_203_F
  GTCGAGGGGATCAACACAAGTGGAATCAAG
  GTCGAGGGGATCAACACAAGTGGAATCAAG
  >scaffold00001_25_26_184_R
  >scaffold00001_25_26_184_R
  GTACCGTCGACAAGCAACTCCTGATCGTC
  GTACCGTCGACAAGCAACTCCTGATCGTC
-
>scaffold00001_26_27_369_F
 
-
ATGAGCGAGCAGGTCTTCCTCCTAC
 
-
>scaffold00001_26_27_706_R
 
-
GAACACGCCGTAGTTGTTCAGCGTGAAG
 
  >scaffold00001_27_28_280_F
  >scaffold00001_27_28_280_F
  TGACGTGAGGGGACTACGGATGTAC
  TGACGTGAGGGGACTACGGATGTAC
  >scaffold00001_27_28_260_R
  >scaffold00001_27_28_260_R
  ACTTCGACAGGTAGGCCTTGCAGAG
  ACTTCGACAGGTAGGCCTTGCAGAG
-
>scaffold00001_28_29_209_F
 
-
TCTACGGTGGGTTGCACTGGTTTAGAGAAG
 
-
>scaffold00001_28_29_357_R
 
-
TACAGCTGAAGGATCTTCTCGTCGAGGACC
 
-
>scaffold00001_29_30_1643_F
 
-
CCGATCCAGGCGAAGATCAGATTGGTC
 
-
>scaffold00001_29_30_767_R
 
-
ATCGACATAGCGCTCCAACAGATCGGTGAC
 
-
>scaffold00001_30_31_237_F
 
-
CACCACACGTTGTACGTCTTCGTGCTCTAC
 
-
>scaffold00001_30_31_286_R
 
-
GACATCCCCCAAGTCTTTCCTCTCATGCTC
 
  >scaffold00001_31_32_566_F
  >scaffold00001_31_32_566_F
  AAACGAGCCGTACAACGGGCAACTG
  AAACGAGCCGTACAACGGGCAACTG
  >scaffold00001_31_32_169_R
  >scaffold00001_31_32_169_R
  ATCTTCCTGACCTCGTCGGTGTGGTACTTC
  ATCTTCCTGACCTCGTCGGTGTGGTACTTC
-
>scaffold00001_32_33_266_F
 
-
AACATTGACAGGGTGGCACGCAGTTCG
 
-
>scaffold00001_32_33_169_R
 
-
TTGTCAATGTAGCGTTGACGGGTGCCTGAC
 
-
>scaffold00001_33_34_795_F
 
-
GTCGACGACGAGAATCCGGTTCATG
 
-
>scaffold00001_33_34_365_R
 
-
GTTCCCTGCTGCTGAACTGGTTCTTCAC
 
  >scaffold00001_34_35_1071_F
  >scaffold00001_34_35_1071_F
  CAGTACGTCATCTTCGACCGGACCATTTG
  CAGTACGTCATCTTCGACCGGACCATTTG
Line 200: Line 124:
  >scaffold00001_36_37_740_R
  >scaffold00001_36_37_740_R
  ATACGGCTTCGTCGAGAACCTGACC
  ATACGGCTTCGTCGAGAACCTGACC
-
>scaffold00001_37_38_498_F
 
-
TTCGACGAACTGTGGCAACGCTTCTCC
 
-
>scaffold00001_37_38_562_R
 
-
AGGACGTCATGCACGGTCAACACAC
 
  >scaffold00001_38_39_612_F
  >scaffold00001_38_39_612_F
  AACATGTCGTTGAGCGCGGCCAGTTTG
  AACATGTCGTTGAGCGCGGCCAGTTTG
  >scaffold00001_38_39_672_R
  >scaffold00001_38_39_672_R
  CTCTTCGACGAGATCATCCAGGTGCTG
  CTCTTCGACGAGATCATCCAGGTGCTG
-
>scaffold00001_39_40_196_F
 
-
CACAATTTCATCGATCAACACAAACGCGAG
 
-
>scaffold00001_39_40_425_R
 
-
ACCTGCAACTGACTCGGCTGGCTATAGCTG
 
-
>scaffold00001_40_41_522_F
 
-
GACTACAAGCCGGAGAACGTGCTCATCTC
 
-
>scaffold00001_40_41_660_R
 
-
GTGCAGAAGGGATAGAGGGTGACGTAC
 
  >scaffold00001_41_42_205_F
  >scaffold00001_41_42_205_F
  ACCGAAGCAGTCGAAGACGGTACAC
  ACCGAAGCAGTCGAAGACGGTACAC
Line 224: Line 136:
  >scaffold00001_42_43_223_R
  >scaffold00001_42_43_223_R
  GTGATCGGTGTGTTCGTGGCTGACCAGTAC
  GTGATCGGTGTGTTCGTGGCTGACCAGTAC
-
>scaffold00001_43_44_440_F
 
-
CTCGGAGGAAAACGGTGAACAGTCAGCAAC
 
-
>scaffold00001_43_44_563_R
 
-
TGAACGCCTCCACCCAGCATTTGATCACAG
 
-
>scaffold00001_44_45_93801_F
 
-
Can't find proper primer
 
-
>scaffold00001_44_45_485_R
 
-
GTGTTGCGATCGGATTCGTAGCTGAC
 
  >scaffold00001_45_46_401_F
  >scaffold00001_45_46_401_F
  GGATCAGCTCCGGATAGTTGAAGTCGTC
  GGATCAGCTCCGGATAGTTGAAGTCGTC
  >scaffold00001_45_46_423_R
  >scaffold00001_45_46_423_R
  ACAACGAGGACTTCGACAACGGCTTCAAGG
  ACAACGAGGACTTCGACAACGGCTTCAAGG
-
>scaffold00001_46_47_370_F
 
-
GTAAACTCCTGATCTCAGCCGCTCTCGTG
 
-
>scaffold00001_46_47_845_R
 
-
CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG
 
  >scaffold00001_47_48_1970_F
  >scaffold00001_47_48_1970_F
  GACGACTACCTGCGTAAACCCTTCGACCTG
  GACGACTACCTGCGTAAACCCTTCGACCTG
Line 256: Line 156:
  >scaffold00001_50_51_860_R
  >scaffold00001_50_51_860_R
  GTGACCAGCGAGTGAATCTTGTCGCGTTG
  GTGACCAGCGAGTGAATCTTGTCGCGTTG
-
>scaffold00001_51_52_1410_F
 
-
GATGTCCACGTCGTACGCCATGCTGTTGTC
 
-
>scaffold00001_51_52_509_R
 
-
CCTGATTCACCTGATTCGTCCACCAGTTC
 
-
>scaffold00001_52_53_361_F
 
-
GTCATCCACACATGGCATTCTGTGACC
 
-
>scaffold00001_52_53_353_R
 
-
AGGATGGCGTAATCCGCGATGATGG
 
-
>scaffold00001_53_54_414_F
 
-
TTCTGGAAGACCATCTGCAGACCGATCTCG
 
-
>scaffold00001_53_54_823_R
 
-
TACGCGAAGGGACGTTAGAGATGGCAC
 
-
>scaffold00001_54_55_1250_F
 
-
CTGGAACCGGATGATGTTGGAGTCCTC
 
-
>scaffold00001_54_55_175_R
 
-
TACCGAGGAACTGATCGACCTCTCC
 
  >scaffold00001_55_56_239_F
  >scaffold00001_55_56_239_F
  CACCTGACCTCTGACAATGAACGCCGAAAG
  CACCTGACCTCTGACAATGAACGCCGAAAG
  >scaffold00001_55_56_206_R
  >scaffold00001_55_56_206_R
  GATTGGCTTCTATACAAACTAAGGTGGCGC
  GATTGGCTTCTATACAAACTAAGGTGGCGC
-
>scaffold00001_56_57_412_F
 
-
GTACTCACGATGGTCCTTCTCCTCGTTG
 
-
>scaffold00001_56_57_184_R
 
-
CAAACCGGTGGTCGGGCTGATCATGTATTC
 
  >scaffold00001_57_58_1204_F
  >scaffold00001_57_58_1204_F
  TCATGCTGTCCGTATCCATTTAGGACCTTC
  TCATGCTGTCCGTATCCATTTAGGACCTTC
  >scaffold00001_57_58_939_R
  >scaffold00001_57_58_939_R
  TTCTACCGTGCCTACTTCGAGACCGAC
  TTCTACCGTGCCTACTTCGAGACCGAC
-
>scaffold00001_58_59_788_F
 
-
CAAAGCAAATCGGAGGGGACATGACG
 
-
>scaffold00001_58_59_229_R
 
-
ACACGTTCGTCCGCAACAACGGATG
 
-
>scaffold00001_59_60_1979_F
 
-
GTGACCAGCCAGGTCTGTTCGAATCTCAC
 
-
>scaffold00001_59_60_625_R
 
-
GAGATCATGTACGCCGACTTCAGCTTCGTG
 
-
>scaffold00001_60_61_755_F
 
-
GTACGGTTTCTGACCAACACCGACTCGAAG
 
-
>scaffold00001_60_61_647_R
 
-
CCAACATCACCATCACGAACCTCGG
 
  >scaffold00001_61_62_285_F
  >scaffold00001_61_62_285_F
  TCAGGTTGAGATCGACCATCGCGAG
  TCAGGTTGAGATCGACCATCGCGAG
Line 304: Line 172:
  >scaffold00001_62_63_367_R
  >scaffold00001_62_63_367_R
  GTTGTAGCCGCCGAAGTCGTTCATGCTGAC
  GTTGTAGCCGCCGAAGTCGTTCATGCTGAC
-
>scaffold00001_63_64_711_F
 
-
ACGTAGTTCCCGACCGTAGAGCAGTTC
 
-
>scaffold00001_63_64_1286_R
 
-
CGATAGGTGAGACTCGATGCATGGTTAGTG
 
-
>scaffold00001_64_65_324_F
 
-
CTGGTGTGCTTTTCGGTTCGGGTAC
 
-
>scaffold00001_64_65_297_R
 
-
AACGCCCACGTCATCCTCGAAGAAC
 
-
>scaffold00001_65_66_1986_F
 
-
GATCAGTCCGGCGATCGACGAGTAGAGAAC
 
-
>scaffold00001_65_66_1292_R
 
-
GTTGCTGCTGGAAGTGGCATGGGAAAC
 
-
>scaffold00001_66_67_428_F
 
-
CTTCGTCACTGGCTGTGTCAAGGTCAAC
 
-
>scaffold00001_66_67_346_R
 
-
ACTGTTCCTGCTGTACTCCTCGGTG
 
  >scaffold00001_67_68_5122_F
  >scaffold00001_67_68_5122_F
  Can't find proper primer
  Can't find proper primer
  >scaffold00001_67_68_0_R
  >scaffold00001_67_68_0_R
  Can't find proper primer
  Can't find proper primer
-
>scaffold00001_68_69_5187_F
 
-
Can't find proper primer
 
-
>scaffold00001_68_69_1373_R
 
-
CTCCTGGTGGGCTCGGTGAAAACCAACATC
 
-
>scaffold00001_69_70_254_F
 
-
CTTCGACATGGAGGCAGTGCTTGAGAACTC
 
-
>scaffold00001_69_70_318_R
 
-
AGTGAGGAGGAACAGTTCTACAATGGTGTG
 
-
>scaffold00001_70_71_240_F
 
-
CATTCGGCGTGGTAAAGACCCACTGGTAC
 
-
>scaffold00001_70_71_233_R
 
-
ATCGATGACCCTCCAAGACAGACCTCATTG
 
  >scaffold00001_71_72_349_F
  >scaffold00001_71_72_349_F
  AACCCACGCATCCCACTCAACGAGCTGAAC
  AACCCACGCATCCCACTCAACGAGCTGAAC
  >scaffold00001_71_72_0_R
  >scaffold00001_71_72_0_R
-
Can't find proper primer
 
-
>scaffold00001_72_73_2964_F
 
-
Can't find proper primer
 
-
>scaffold00001_72_73_0_R
 
  Can't find proper primer
  Can't find proper primer
  >scaffold00001_73_74_2968_F
  >scaffold00001_73_74_2968_F
  Can't find proper primer
  Can't find proper primer
  >scaffold00001_73_74_0_R
  >scaffold00001_73_74_0_R
-
Can't find proper primer
 
-
>scaffold00001_74_75_356_F
 
-
GTGTTCACCGGCGTGATGTACAACGACTAC
 
-
>scaffold00001_74_75_0_R
 
  Can't find proper primer
  Can't find proper primer
  >scaffold00001_75_76_788_F
  >scaffold00001_75_76_788_F
Line 360: Line 192:
  >scaffold00001_76_77_535_R
  >scaffold00001_76_77_535_R
  ATCTACAACCACCTGGGCGGCAAGGAGAAC
  ATCTACAACCACCTGGGCGGCAAGGAGAAC
-
>scaffold00001_77_78_624_F
 
-
AATCTCATGGGGTGCCTTCGTCGATCGGTGAG
 
-
>scaffold00001_77_78_349_R
 
-
CTCGGCAACCGACTGTGGAACAACATC
 
-
>scaffold00001_78_79_195_F
 
-
GTAGTTGCACGAGACGGCCATTACCTTG
 
-
>scaffold00001_78_79_276_R
 
-
GAAATGCCGTCACTCTCCGTAGATGTCC
 
-
>scaffold00001_79_80_301_F
 
-
TACGTTTGGCTCAACACCCCACGCGAGATG
 
-
>scaffold00001_79_80_212_R
 
-
GTCGCTGATCATGGTTCGGTAGACGATGTC
 
  >scaffold00001_80_81_426_F
  >scaffold00001_80_81_426_F
  CACTGCCCAAACCGATCTATCCGACGTTC
  CACTGCCCAAACCGATCTATCCGACGTTC
  >scaffold00001_80_81_202_R
  >scaffold00001_80_81_202_R
  GATCCAAGCTGTCTTAACGAGGAGTTGCC
  GATCCAAGCTGTCTTAACGAGGAGTTGCC
-
>scaffold00001_81_82_751_F
 
-
CAAATCCGGGCACCTCGAACTCAAG
 
-
>scaffold00001_81_82_233_R
 
-
AACACCACCACCCCCGAGTAGCAGACAACAAC
 
-
>scaffold00001_82_83_253_F
 
-
CAAGGTCATCGTCAGCGGTGGATTG
 
-
>scaffold00001_82_83_302_R
 
-
AGATCCGGATACTGCGCTGGGAAAC
 
-
>scaffold00001_83_84_243_F
 
-
GTGGGGCAAGTGCTGGAGTTTGATTCAC
 
-
>scaffold00001_83_84_182_R
 
-
GTGGACGGACCAGTCGTGATGTTCGATTC
 
-
>scaffold00001_84_85_414_F
 
-
GTTTACCCTCGATCCGGCAGATGAAGC
 
-
>scaffold00001_84_85_726_R
 
-
GACCCGTCCAACACCTTCGATCAGAGCTTC
 
-
>scaffold00001_85_86_202_F
 
-
GTTTAGGAAGTGTGGACACGGCCGGAAGTC
 
-
>scaffold00001_85_86_191_R
 
-
CCGAAGTGTGCAAACGTTGTCTTTACGTC
 
-
>scaffold00001_86_87_218_F
 
-
TCAACAACCCAGTGAACACCGAGGACGTAC
 
-
>scaffold00001_86_87_245_R
 
-
CATTGAGAAAGCGATGTACCGCCACGAGAC
 
  >scaffold00001_87_88_204_F
  >scaffold00001_87_88_204_F
  GAAGTGATCACGCGATCCACGGGATTCCAC
  GAAGTGATCACGCGATCCACGGGATTCCAC
Line 408: Line 204:
  >scaffold00001_88_89_463_R
  >scaffold00001_88_89_463_R
  CAGGTGTTCAACCTCGCCCAGACCTTCTAC
  CAGGTGTTCAACCTCGCCCAGACCTTCTAC
-
  >scaffold00001_89_90_613_F
+
  >scaffold00003_3_4_218_F
-
  ATGACCTTCGTGAAACTGACCTTCTCGCTG
+
  ACAAGCTCATTGCCTACTAGATCGACTTCG
-
  >scaffold00001_89_90_528_R
+
  >scaffold00003_3_4_1054_R
-
  GACAAGGACTCCGCCAAGAAGGACGAATC
+
  GCATCGTCCAACGGTAGCCTTCTTTC
-
 
+
>scaffold00003_7_8_365_F
 +
ATCCGACGAGTACGACATCCACCTG
 +
>scaffold00003_7_8_601_R
 +
CAGACCAACTACGCCACAAGCAAGG
 +
>scaffold00003_8_9_472_F
 +
CTGTTCCAGACCTTCCTCACCGTGCTTGAC
 +
>scaffold00003_8_9_218_R
 +
CTTATGCCCTGACAGAAGGTGCAAGATCAG
 +
>scaffold00003_10_11_1436_F
 +
TGGTCCTGCCGTACTTCCTGATCACCACTG
 +
>scaffold00003_10_11_232_R
 +
GATGGGACAGACTTCGTACACCGGTTG
 +
>scaffold00003_11_12_266_F
 +
CTGTATTGATGATCCACTCGCTGCCGCTC
 +
>scaffold00003_11_12_1184_R
 +
TACAGTGATCGGCCGTTGTTGGAGTGTGTG
 +
>scaffold00003_17_18_314_F
 +
TCTGAACGAGACGGAATGGGAGATCCTCAC
 +
>scaffold00003_17_18_222_R
 +
GACATCACCATCCAGGCAGAGAACATGCTG
 +
>scaffold00003_19_20_655_F
 +
GAAGGAACGGCAACTTTCGGTCACGGTAC
 +
>scaffold00003_19_20_485_R
 +
CAAGGAGACCGTCTACAAGATCTGGCATC
 +
>scaffold00003_23_24_235_F
 +
CCCAGGGAATATAACGATGGTTCAGGTCAG
 +
>scaffold00003_23_24_1163_R
 +
GAACTGCTGGTTCATCGAATGGTCGTTGAC
 +
>scaffold00003_24_25_268_F
 +
CTCGAAGTCCCGGTCAAGAAGATCCTCAC
 +
>scaffold00003_24_25_666_R
 +
TATCGTTCGCGTCGATGGCCGACAACATTG
 +
>scaffold00003_26_27_1270_F
 +
GAAATCAAGATCCTGAACCAGTCCCGGAAG
 +
>scaffold00003_26_27_244_R
 +
CCCGATTCCTTGTCCTGGAACAGATAGAAG
 +
>scaffold00003_27_28_833_F
 +
GTACTCGCCGACTTTCCCGTTCTCATC
 +
>scaffold00003_27_28_474_R
 +
GTACACCTCGTGCTCGGGACACAAATGGTC
 +
>scaffold00003_28_29_210_F
 +
GTACACGTGCCGTTCCCGTTTCTGGTGAAC
 +
>scaffold00003_28_29_173_R
 +
ACGGCGTCACGAACAACACCTACTG
 +
>scaffold00003_30_31_256_F
 +
TTCCGGGGATGAGTAGCCGACCAATTGTCC
 +
>scaffold00003_30_31_1239_R
 +
GATCACCGGTTGGTAGACCAGATCCAG
 +
>scaffold00003_31_32_351_F
 +
CACAAATTGAACTTGAGCTGGGATGTCAGG
 +
>scaffold00003_31_32_565_R
 +
CAGGATGACAGTGTGAGTCAGCCATGGAC
 +
>scaffold00003_35_36_707_F
 +
AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
 +
>scaffold00003_35_36_243_R
 +
CAGGTTGAACATCTCCTGGACCAGCGAAC
 +
>scaffold00003_42_43_317_F
 +
GTTCCTGTCCTCGTGCAGCACAATTCGATC
 +
>scaffold00003_42_43_298_R
 +
ACCGGGGATCATCGTGGTCTTGTAC
 +
>scaffold00003_43_44_737_F
 +
ATCGTTCGCAGCGGCATCGATTCAC
 +
>scaffold00003_43_44_992_R
 +
TGAAGCTGACCAAGCTGGTGGTCTC
 +
>scaffold00003_45_46_422_F
 +
GTGATGCCCTCGGATCCCAGTTCTTCGTAC
 +
>scaffold00003_45_46_399_R
 +
AACTCGGTCAAGCTCGAGTTCCTGGTG
 +
>scaffold00003_47_48_427_F
 +
CATCACGGCGAACAACAACCAGACCTAC
 +
>scaffold00003_47_48_271_R
 +
GAACAACGGCATGAGGTCCTTGAACATGAC
 +
>scaffold00003_48_49_532_F
 +
GAAGGGTCAACAGTGAGATCGCCTGGTTC
 +
>scaffold00003_48_49_741_R
 +
TGTAGCTGCCCTTCTCCTCCTGTTC
 +
>scaffold00003_49_50_208_F
 +
GTGGTGTGCATCGTGCAGATCCCGTTCTAC
 +
>scaffold00003_49_50_975_R
 +
AACCCGGAGCCGAAGAGTTCGAGAAAC
 +
>scaffold00003_50_51_1445_F
 +
TGGCGTACCTGGAGAAGGTGGTTTC
 +
>scaffold00003_50_51_439_R
 +
CAATGGCACCCTCACATATGGCAGATAATTG
 +
>scaffold00004_8_9_447_F
 +
GAACCTGCTGTTCATGGTCGGTCTG
 +
>scaffold00004_8_9_234_R
 +
ACAGTGGTCTCCGTCTACAGTCCAC
 +
>scaffold00004_12_13_750_F
 +
TCGATGGACATGCTCTCCTCCGAGATCGTC
 +
>scaffold00004_12_13_895_R
 +
TATGCGAGCGTCCGAAAAAGATCGACCAGC
 +
>scaffold00004_13_14_550_F
 +
GAGCACATCAAGATCACTGACAATGGGATC
 +
>scaffold00004_13_14_243_R
 +
AACACTCCGAAATCCGCTGGCTCTC
 +
>scaffold00004_14_15_556_F
 +
CTGGTGGGACTCGTTGAGCATCCAATG
 +
>scaffold00004_14_15_220_R
 +
CCGAGGGAAAGAACCGGACATTGTG
 +
>scaffold00004_15_16_1515_F
 +
CTTCACCGGCCTGTTCTTCGTCATCAC
 +
>scaffold00004_15_16_167_R
 +
CAGTACCTGTGGCGTCACACGCGATAAGAG
 +
>scaffold00004_16_17_236_F
 +
GGTTCGACACCAGGATTTCGAGGTCAC
 +
>scaffold00004_16_17_467_R
 +
GATCTGGTTCCACATCTGTCCCTGGTG
 +
>scaffold00004_18_19_226_F
 +
GTGTGATCGACACGGTTACGTCGAAG
 +
>scaffold00004_18_19_492_R
 +
TTAACAGGGGGCCCTTCCTTCAACACGGTC
 +
>scaffold00004_20_21_2075_F
 +
AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
 +
>scaffold00004_20_21_521_R
 +
TGAAGATCACCCAGGATCTCAAGGAGTACG
 +
>scaffold00004_21_22_386_F
 +
GAAGACTTCGCCACCGAATACCTCAGC
 +
>scaffold00004_21_22_182_R
 +
TTCGACGTACACACCACCCTCGCCGTAATC
 +
>scaffold00004_23_24_720_F
 +
TCCACCGGAACATGGGTGATACTCGAC
 +
>scaffold00004_23_24_552_R
 +
GTTGCCTTTGCACAGGTAGATCCCG
 +
>scaffold00004_24_25_667_F
 +
CAAGCCATGTCGAAGCAGCACTACTC
 +
>scaffold00004_24_25_322_R
 +
AAGCACCTGCTGTTCACAAGCGAACTGATC
 +
>scaffold00004_25_26_542_F
 +
GCAGATCGACGAGTACCTGGAGTTCTAC
 +
>scaffold00004_25_26_247_R
 +
AGGAGTCCGACCATGAAGATCAGGAAGATC
 +
>scaffold00004_29_30_278_F
 +
CATTTGTGACACCTGTCCAGCAGTACG
 +
>scaffold00004_29_30_264_R
 +
TCTGGTGAGCCGACAGGTATTCCTC
===primer part 2 - scaffold00003, scaffold00004===
===primer part 2 - scaffold00003, scaffold00004===

Revision as of 01:54, 15 February 2011

Contents

Primer Naming Format

Scf&SolCtg primer format

Gap primer format

ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R

                    CONTIG 1                                                     CONTIG 2
                               ------>
        5'                           3'  
--------CTCAGGTCAGGCAGGCGTGATGATCTCTTC----------------NNNNNNNNN----------------------------------------
                                                                       GTTAGAGGAGAGTGCGGCTGGGCTCCAATCAT
                                                                       3'                             5'
                                                                       <-------

                                            1105bp               529bp

List of primer

primers of scaffold & sole contigs

scf1

>scaffold00001_223_H
TTGCGTGGGTTGAAGCAGAGCAAAGCAGTT
>scaffold00001_2007_T
ATGACCGCCAGGTGACTCTGCAACCCACTC

scf2

scf3

>scaffold00003_1985_H
CCGATCTCGTCATGTCCAGCCTGGATGAGT
>scaffold00003_1004_T
CCAAGTCCGACGGCTCGAAGTTCGGCAAGA

scf4

>scaffold00004_151_H
GTTGATCTGCTGAGCGTGATTCGGATAGTT
>scaffold00004_548_T
CAACATCACCTCGCCGAAGACCGTCTATCT

ctg205

>23s5s_H
TGGTGTGGTGTTGTTTGATATGTTGATAGG
>23s5s_T
ACGTCTCAGCCTACATGTGCCACGGATTTG

ctg231

>16s_H
CGTGTTACTCACCCGTTCGCCGCTCGAGTA
>16s_T
TGGCGATTGGGACGAAGTCGTAACAAGGTA


primer intergenic

>scaffold00001_2_3_751_F
CAGAACTGGAGAACGTCCATGCTCGAGAAC
>scaffold00001_2_3_1360_R
TCGATCACCAGCTCACGGAAGTCCTCGTTG
>scaffold00001_3_4_586_F
GTCAGTGCCGATCAGGATGTTGTCCAC
>scaffold00001_3_4_183_R
ATCAACTGCCAGAACTCGTCGGTCCTCATG
>scaffold00001_4_5_568_F
TACGTCGAGGAACTGGGCGACTTCAAGACC
>scaffold00001_4_5_838_R
CATGTCGTCGATCTGCACGTTGACCAG
>scaffold00001_9_10_1135_F
AGACCGGATTGAGTGCTTCCTCTGCTGGTG
>scaffold00001_9_10_1408_R
CGACCAATTTCCGACGAGGTGACATTC
>scaffold00001_11_12_354_F
AAGGTCAGGATGGCGGTGAACACCTTGGTC
>scaffold00001_11_12_345_R
ATGTTGTCGCTGACCTTCGCGATACGGGAG
>scaffold00001_12_13_1313_F
TTTCAGGCTTTGAGGATCGAGGTAAACAGG
>scaffold00001_12_13_315_R
TCGCATCTTCTCAGCCAGTTCCGAG
>scaffold00001_15_16_126613_F
Can't find proper primer
>scaffold00001_15_16_569_R
CTTCTACCACGACACACACACCGATCC
>scaffold00001_17_18_225_F
GATCGTGACGAACGTGAAGGTCCAC
>scaffold00001_17_18_408_R
CAGATCCGGTACAGCTCCTTGTATTCCAG
>scaffold00001_18_19_320_F
CAGATAGATGGTGAAGTCAGGACGACGG
>scaffold00001_18_19_177_R
ATGAACTTTCCACCGATGCCGGTCGTCTTC
>scaffold00001_21_22_243_F
AAGGACGGACGTGGACATCGATCAGCAATC
>scaffold00001_21_22_1379_R
AGGACTCCTGTACGTCGATCACGACGAGTG
>scaffold00001_23_24_410_F
ACTGACCACGGTGCAGCTCTACTTCAC
>scaffold00001_23_24_1184_R
AGCGTGTAGTCGGCGGTATACACCTTC
>scaffold00001_25_26_203_F
GTCGAGGGGATCAACACAAGTGGAATCAAG
>scaffold00001_25_26_184_R
GTACCGTCGACAAGCAACTCCTGATCGTC
>scaffold00001_27_28_280_F
TGACGTGAGGGGACTACGGATGTAC
>scaffold00001_27_28_260_R
ACTTCGACAGGTAGGCCTTGCAGAG
>scaffold00001_31_32_566_F
AAACGAGCCGTACAACGGGCAACTG
>scaffold00001_31_32_169_R
ATCTTCCTGACCTCGTCGGTGTGGTACTTC
>scaffold00001_34_35_1071_F
CAGTACGTCATCTTCGACCGGACCATTTG
>scaffold00001_34_35_2096_R
AGTCGTCGTCGTCGAACATCGTGAC
>scaffold00001_35_36_685_F
GTCGTTCAGGTTCAGGTAGAACTCGGTGAC
>scaffold00001_35_36_0_R
Can't find proper primer
>scaffold00001_36_37_1073_F
GATCGGTTTGCCGCGGTCGTTGTATTCGAC
>scaffold00001_36_37_740_R
ATACGGCTTCGTCGAGAACCTGACC
>scaffold00001_38_39_612_F
AACATGTCGTTGAGCGCGGCCAGTTTG
>scaffold00001_38_39_672_R
CTCTTCGACGAGATCATCCAGGTGCTG
>scaffold00001_41_42_205_F
ACCGAAGCAGTCGAAGACGGTACAC
>scaffold00001_41_42_339_R
GTCTTCTTCTGGCCCCGCAACGAGATCATG
>scaffold00001_42_43_389_F
TAAAATCTGTGATCTGGGCTAACCTTGGGG
>scaffold00001_42_43_223_R
GTGATCGGTGTGTTCGTGGCTGACCAGTAC
>scaffold00001_45_46_401_F
GGATCAGCTCCGGATAGTTGAAGTCGTC
>scaffold00001_45_46_423_R
ACAACGAGGACTTCGACAACGGCTTCAAGG
>scaffold00001_47_48_1970_F
GACGACTACCTGCGTAAACCCTTCGACCTG
>scaffold00001_47_48_1044_R
CACCAATCTGCTTGTGGGTCTGGTGCTGAC
>scaffold00001_48_49_1639_F
GATGTTCATCGACCTGGAGAACGTGC
>scaffold00001_48_49_244_R
CATCTCTCCCGTCAGCTCATCGTTCGAAG
>scaffold00001_49_50_1297_F
CAGGGTTTCCTGAGCCACCTTGAAC
>scaffold00001_49_50_822_R
CTGTTCTCCCAGGACCCGATCTTCAACTC
>scaffold00001_50_51_227_F
CGTGCCATGTTCTGAACGAGAACGATC
>scaffold00001_50_51_860_R
GTGACCAGCGAGTGAATCTTGTCGCGTTG
>scaffold00001_55_56_239_F
CACCTGACCTCTGACAATGAACGCCGAAAG
>scaffold00001_55_56_206_R
GATTGGCTTCTATACAAACTAAGGTGGCGC
>scaffold00001_57_58_1204_F
TCATGCTGTCCGTATCCATTTAGGACCTTC
>scaffold00001_57_58_939_R
TTCTACCGTGCCTACTTCGAGACCGAC
>scaffold00001_61_62_285_F
TCAGGTTGAGATCGACCATCGCGAG
>scaffold00001_61_62_944_R
TTGCACCATCAGCTCAGCATCGTCTTCGAC
>scaffold00001_62_63_898_F
CTGATGATCAAGGTGTTGCGGCTGTC
>scaffold00001_62_63_367_R
GTTGTAGCCGCCGAAGTCGTTCATGCTGAC
>scaffold00001_67_68_5122_F
Can't find proper primer
>scaffold00001_67_68_0_R
Can't find proper primer
>scaffold00001_71_72_349_F
AACCCACGCATCCCACTCAACGAGCTGAAC
>scaffold00001_71_72_0_R
Can't find proper primer
>scaffold00001_73_74_2968_F
Can't find proper primer
>scaffold00001_73_74_0_R
Can't find proper primer
>scaffold00001_75_76_788_F
GTCGACGTTTCATCACGTCACTGGGTG
>scaffold00001_75_76_0_R
Can't find proper primer
>scaffold00001_76_77_1854_F
GTGATCAAGGTGATCGAAGCCCTGC
>scaffold00001_76_77_535_R
ATCTACAACCACCTGGGCGGCAAGGAGAAC
>scaffold00001_80_81_426_F
CACTGCCCAAACCGATCTATCCGACGTTC
>scaffold00001_80_81_202_R
GATCCAAGCTGTCTTAACGAGGAGTTGCC
>scaffold00001_87_88_204_F
GAAGTGATCACGCGATCCACGGGATTCCAC
>scaffold00001_87_88_267_R
AGCTTCCAGACCCTGGTCAACAGTC
>scaffold00001_88_89_1973_F
AGTAGCCGATGACCCCGTTGAGTTG
>scaffold00001_88_89_463_R
CAGGTGTTCAACCTCGCCCAGACCTTCTAC
>scaffold00003_3_4_218_F
ACAAGCTCATTGCCTACTAGATCGACTTCG
>scaffold00003_3_4_1054_R
GCATCGTCCAACGGTAGCCTTCTTTC
>scaffold00003_7_8_365_F
ATCCGACGAGTACGACATCCACCTG
>scaffold00003_7_8_601_R
CAGACCAACTACGCCACAAGCAAGG
>scaffold00003_8_9_472_F
CTGTTCCAGACCTTCCTCACCGTGCTTGAC
>scaffold00003_8_9_218_R
CTTATGCCCTGACAGAAGGTGCAAGATCAG
>scaffold00003_10_11_1436_F
TGGTCCTGCCGTACTTCCTGATCACCACTG
>scaffold00003_10_11_232_R
GATGGGACAGACTTCGTACACCGGTTG
>scaffold00003_11_12_266_F
CTGTATTGATGATCCACTCGCTGCCGCTC
>scaffold00003_11_12_1184_R
TACAGTGATCGGCCGTTGTTGGAGTGTGTG
>scaffold00003_17_18_314_F
TCTGAACGAGACGGAATGGGAGATCCTCAC
>scaffold00003_17_18_222_R
GACATCACCATCCAGGCAGAGAACATGCTG
>scaffold00003_19_20_655_F
GAAGGAACGGCAACTTTCGGTCACGGTAC
>scaffold00003_19_20_485_R
CAAGGAGACCGTCTACAAGATCTGGCATC
>scaffold00003_23_24_235_F
CCCAGGGAATATAACGATGGTTCAGGTCAG
>scaffold00003_23_24_1163_R
GAACTGCTGGTTCATCGAATGGTCGTTGAC
>scaffold00003_24_25_268_F
CTCGAAGTCCCGGTCAAGAAGATCCTCAC
>scaffold00003_24_25_666_R
TATCGTTCGCGTCGATGGCCGACAACATTG
>scaffold00003_26_27_1270_F
GAAATCAAGATCCTGAACCAGTCCCGGAAG
>scaffold00003_26_27_244_R
CCCGATTCCTTGTCCTGGAACAGATAGAAG
>scaffold00003_27_28_833_F
GTACTCGCCGACTTTCCCGTTCTCATC
>scaffold00003_27_28_474_R
GTACACCTCGTGCTCGGGACACAAATGGTC
>scaffold00003_28_29_210_F
GTACACGTGCCGTTCCCGTTTCTGGTGAAC
>scaffold00003_28_29_173_R
ACGGCGTCACGAACAACACCTACTG
>scaffold00003_30_31_256_F
TTCCGGGGATGAGTAGCCGACCAATTGTCC
>scaffold00003_30_31_1239_R
GATCACCGGTTGGTAGACCAGATCCAG
>scaffold00003_31_32_351_F
CACAAATTGAACTTGAGCTGGGATGTCAGG
>scaffold00003_31_32_565_R
CAGGATGACAGTGTGAGTCAGCCATGGAC
>scaffold00003_35_36_707_F
AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
>scaffold00003_35_36_243_R
CAGGTTGAACATCTCCTGGACCAGCGAAC
>scaffold00003_42_43_317_F
GTTCCTGTCCTCGTGCAGCACAATTCGATC
>scaffold00003_42_43_298_R
ACCGGGGATCATCGTGGTCTTGTAC
>scaffold00003_43_44_737_F
ATCGTTCGCAGCGGCATCGATTCAC
>scaffold00003_43_44_992_R
TGAAGCTGACCAAGCTGGTGGTCTC
>scaffold00003_45_46_422_F
GTGATGCCCTCGGATCCCAGTTCTTCGTAC
>scaffold00003_45_46_399_R
AACTCGGTCAAGCTCGAGTTCCTGGTG
>scaffold00003_47_48_427_F
CATCACGGCGAACAACAACCAGACCTAC
>scaffold00003_47_48_271_R
GAACAACGGCATGAGGTCCTTGAACATGAC
>scaffold00003_48_49_532_F
GAAGGGTCAACAGTGAGATCGCCTGGTTC
>scaffold00003_48_49_741_R
TGTAGCTGCCCTTCTCCTCCTGTTC
>scaffold00003_49_50_208_F
GTGGTGTGCATCGTGCAGATCCCGTTCTAC
>scaffold00003_49_50_975_R
AACCCGGAGCCGAAGAGTTCGAGAAAC
>scaffold00003_50_51_1445_F
TGGCGTACCTGGAGAAGGTGGTTTC
>scaffold00003_50_51_439_R
CAATGGCACCCTCACATATGGCAGATAATTG
>scaffold00004_8_9_447_F
GAACCTGCTGTTCATGGTCGGTCTG
>scaffold00004_8_9_234_R
ACAGTGGTCTCCGTCTACAGTCCAC
>scaffold00004_12_13_750_F
TCGATGGACATGCTCTCCTCCGAGATCGTC
>scaffold00004_12_13_895_R
TATGCGAGCGTCCGAAAAAGATCGACCAGC
>scaffold00004_13_14_550_F
GAGCACATCAAGATCACTGACAATGGGATC
>scaffold00004_13_14_243_R
AACACTCCGAAATCCGCTGGCTCTC
>scaffold00004_14_15_556_F
CTGGTGGGACTCGTTGAGCATCCAATG
>scaffold00004_14_15_220_R
CCGAGGGAAAGAACCGGACATTGTG
>scaffold00004_15_16_1515_F
CTTCACCGGCCTGTTCTTCGTCATCAC
>scaffold00004_15_16_167_R
CAGTACCTGTGGCGTCACACGCGATAAGAG
>scaffold00004_16_17_236_F
GGTTCGACACCAGGATTTCGAGGTCAC
>scaffold00004_16_17_467_R
GATCTGGTTCCACATCTGTCCCTGGTG
>scaffold00004_18_19_226_F
GTGTGATCGACACGGTTACGTCGAAG
>scaffold00004_18_19_492_R
TTAACAGGGGGCCCTTCCTTCAACACGGTC
>scaffold00004_20_21_2075_F
AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
>scaffold00004_20_21_521_R
TGAAGATCACCCAGGATCTCAAGGAGTACG
>scaffold00004_21_22_386_F
GAAGACTTCGCCACCGAATACCTCAGC
>scaffold00004_21_22_182_R
TTCGACGTACACACCACCCTCGCCGTAATC
>scaffold00004_23_24_720_F
TCCACCGGAACATGGGTGATACTCGAC
>scaffold00004_23_24_552_R
GTTGCCTTTGCACAGGTAGATCCCG
>scaffold00004_24_25_667_F
CAAGCCATGTCGAAGCAGCACTACTC
>scaffold00004_24_25_322_R
AAGCACCTGCTGTTCACAAGCGAACTGATC
>scaffold00004_25_26_542_F
GCAGATCGACGAGTACCTGGAGTTCTAC
>scaffold00004_25_26_247_R
AGGAGTCCGACCATGAAGATCAGGAAGATC
>scaffold00004_29_30_278_F
CATTTGTGACACCTGTCCAGCAGTACG
>scaffold00004_29_30_264_R
TCTGGTGAGCCGACAGGTATTCCTC

primer part 2 - scaffold00003, scaffold00004

>scaffold00003_1_2_276_F
GACGTGTACATCTCGAACATCTCGCGG
>scaffold00003_1_2_387_R
AAAACGGGCGTTCACCGCATGGTAC
>scaffold00003_2_3_232_F
CAAGGGAGAGTTCCGTTACGACCACAG
>scaffold00003_2_3_460_R
TTGATCAGCCGGCACAGCAACAAGGTGGTG
>scaffold00003_3_4_218_F
ACAAGCTCATTGCCTACTAGATCGACTTCG
>scaffold00003_3_4_1054_R
GCATCGTCCAACGGTAGCCTTCTTTC
>scaffold00003_4_5_574_F
ATCGACCTGGTGCACGCGAACAACTCCAAG
>scaffold00003_4_5_289_R
GAAGAAGGCGTTGGAGTGCCACAGTTG
>scaffold00003_5_6_195_F
ATCTGTTGCCGAATTGGCCTGTACTAGATC
>scaffold00003_5_6_263_R
ATGGCATCCCTCAACCACTACCACC
>scaffold00003_6_7_918_F
ATGCTCAGTCCGGGAGTACAGCTCGGTAAC
>scaffold00003_6_7_245_R
GCATGCTGTTGTTGATGCTGTCCTTCAG
>scaffold00003_7_8_365_F
ATCCGACGAGTACGACATCCACCTG
>scaffold00003_7_8_601_R
CAGACCAACTACGCCACAAGCAAGG
>scaffold00003_8_9_472_F
CTGTTCCAGACCTTCCTCACCGTGCTTGAC
>scaffold00003_8_9_218_R
CTTATGCCCTGACAGAAGGTGCAAGATCAG
>scaffold00003_9_10_376_F
CATGCACAAGCAGGACCTCATCGAG
>scaffold00003_9_10_212_R
TTTCCGTTGAGCCGCCACTCGTACTTGTAG
>scaffold00003_10_11_1436_F
TGGTCCTGCCGTACTTCCTGATCACCACTG
>scaffold00003_10_11_232_R
GATGGGACAGACTTCGTACACCGGTTG
>scaffold00003_11_12_266_F
CTGTATTGATGATCCACTCGCTGCCGCTC
>scaffold00003_11_12_1184_R
TACAGTGATCGGCCGTTGTTGGAGTGTGTG
>scaffold00003_12_13_2070_F
CTCCAGCAGGGTTTCATCGATTCGGTAG
>scaffold00003_12_13_324_R
CTGGACCAGTTCACCCTCCAGATCATG
>scaffold00003_13_14_463_F
CTGGTGTCACACAACGAAAAGGACCTGAC
>scaffold00003_13_14_304_R
GAAGAAGTCGTCCTTCTCCTTGACCGACAG
>scaffold00003_14_15_137305_F
Can't find proper primer
>scaffold00003_14_15_518_R
AATACCGCTACGGTGCTGGAACCGATGTAC
>scaffold00003_15_16_227_F
GTTTCTGGACAAGTACGGTTTCGGGACC
>scaffold00003_15_16_170_R
CTTGAGGCGCTCACTGATGCACTTAAC
>scaffold00003_16_17_319_F
TCACGTGATCTCCTGATAGGTAGACAGACG
>scaffold00003_16_17_503_R
TACCTGTGGCCCCAAAGCTACTACCTG
>scaffold00003_17_18_314_F
TCTGAACGAGACGGAATGGGAGATCCTCAC
>scaffold00003_17_18_222_R
GACATCACCATCCAGGCAGAGAACATGCTG
>scaffold00003_18_19_746_F
GTTGCTTCGAATCTGGTGACGGGAG
>scaffold00003_18_19_471_R
GTATACCGCAACATCGGCGAAATGAAGTC
>scaffold00003_19_20_655_F
GAAGGAACGGCAACTTTCGGTCACGGTAC
>scaffold00003_19_20_485_R
CAAGGAGACCGTCTACAAGATCTGGCATC
>scaffold00003_20_21_294_F
ATCAGCCCCGAGCAATTGACCGACCTGAAC
>scaffold00003_20_21_200_R
GAAGATCGGCAGCTCCATCGTCTCATC
>scaffold00003_21_22_1034_F
GAAGTAACCGGTGGTGTTTTCGGCCAG
>scaffold00003_21_22_354_R
ATCTCGCCGGTTACAAGCAGATCCTC
>scaffold00003_22_23_1299_F
TTCAGATGGTTAAGGACAGCTTCATGAGCG
>scaffold00003_22_23_368_R
ACGTGATCGGTGGGAACTTCGACTC
>scaffold00003_23_24_235_F
CCCAGGGAATATAACGATGGTTCAGGTCAG
>scaffold00003_23_24_1163_R
GAACTGCTGGTTCATCGAATGGTCGTTGAC
>scaffold00003_24_25_268_F
CTCGAAGTCCCGGTCAAGAAGATCCTCAC
>scaffold00003_24_25_666_R
TATCGTTCGCGTCGATGGCCGACAACATTG
>scaffold00003_25_26_360_F
GAAAGGCTGACCATCTACCCGGAGTAC
>scaffold00003_25_26_636_R
TGATCCACGTGTCCGTACATCATCGTG
>scaffold00003_26_27_1270_F
GAAATCAAGATCCTGAACCAGTCCCGGAAG
>scaffold00003_26_27_244_R
CCCGATTCCTTGTCCTGGAACAGATAGAAG
>scaffold00003_27_28_833_F
GTACTCGCCGACTTTCCCGTTCTCATC
>scaffold00003_27_28_474_R
GTACACCTCGTGCTCGGGACACAAATGGTC
>scaffold00003_28_29_210_F
GTACACGTGCCGTTCCCGTTTCTGGTGAAC
>scaffold00003_28_29_173_R
ACGGCGTCACGAACAACACCTACTG
>scaffold00003_29_30_703_F
TCAGCGATTCAGAGGTCCCGATCAGAGGTC
>scaffold00003_29_30_1341_R
TACCTGCATACCGTACGTGGAGTCG
>scaffold00003_30_31_256_F
TTCCGGGGATGAGTAGCCGACCAATTGTCC
>scaffold00003_30_31_1239_R
GATCACCGGTTGGTAGACCAGATCCAG
>scaffold00003_31_32_351_F
CACAAATTGAACTTGAGCTGGGATGTCAGG
>scaffold00003_31_32_565_R
CAGGATGACAGTGTGAGTCAGCCATGGAC
>scaffold00003_32_33_376_F
AGATCGACGAGCTCAACGTCTATCCG
>scaffold00003_32_33_1069_R
GTCATTCGACACCCACCAGCAACGGATAG
>scaffold00003_33_34_1969_F
GTCCTCAAGCATCGCAAGCGCAAGGAAAAG
>scaffold00003_33_34_485_R
CTCCAACTGCTCGGAGAGGAACTTGTAAC
>scaffold00003_34_35_491_F
GAACGAGACCAGGAGACATAGTGGCGAGTC
>scaffold00003_34_35_222_R
ATCTCCGCAGTCATATCGGTACGGG
>scaffold00003_35_36_707_F
AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
>scaffold00003_35_36_243_R
CAGGTTGAACATCTCCTGGACCAGCGAAC
>scaffold00003_36_37_355_F
CTGCTCTTCCTCGGCATGCTCATGTTC
>scaffold00003_36_37_430_R
ATAGCCCGCCCACTGCATGACAAAC
>scaffold00003_37_38_542_F
CGACTGGGAGTACTACATCGGTAACACC
>scaffold00003_37_38_565_R
GCACTGCGTGAACTCCCTTTCCATC
>scaffold00003_38_39_791_F
AACTGTCGACGAAGAACCGCGTCATGAGAG
>scaffold00003_38_39_613_R
GTACGAGCAGTTCACCAGTTGCGTG
>scaffold00003_39_40_351_F
CACTTGTCTTCTCCTCCGGATACCTGG
>scaffold00003_39_40_333_R
CTGATCCGTCATGGCGCACATTCCACGATG
>scaffold00003_40_41_416_F
GTGGTAGGTCTGTGCCTGGTGGTTATG
>scaffold00003_40_41_370_R
AAGTAGTGCGGCTCCTGCCATCTTCGTTTG
>scaffold00003_41_42_419_F
GTAGGTCTGTGCCTGGTGCTGGTGGTTATG
>scaffold00003_41_42_166_R
GTACCGCCAACACGACGACTCATTC
>scaffold00003_42_43_317_F
GTTCCTGTCCTCGTGCAGCACAATTCGATC
>scaffold00003_42_43_298_R
ACCGGGGATCATCGTGGTCTTGTAC
>scaffold00003_43_44_737_F
ATCGTTCGCAGCGGCATCGATTCAC
>scaffold00003_43_44_992_R
TGAAGCTGACCAAGCTGGTGGTCTC
>scaffold00003_44_45_1117_F
CTCTGTGAGTACGTGGAACAGGCGATG
>scaffold00003_44_45_224_R
AAGATCACCACGGCGTCGACACAGCTCAAC
>scaffold00003_45_46_422_F
GTGATGCCCTCGGATCCCAGTTCTTCGTAC
>scaffold00003_45_46_399_R
AACTCGGTCAAGCTCGAGTTCCTGGTG
>scaffold00003_46_47_258_F
GACCTGCTGAGTGGATCATCAACCTATCG
>scaffold00003_46_47_510_R
GTTGTAACCCATGGCTGACGAAGTAGGG
>scaffold00003_47_48_427_F
CATCACGGCGAACAACAACCAGACCTAC
>scaffold00003_47_48_271_R
GAACAACGGCATGAGGTCCTTGAACATGAC
>scaffold00003_48_49_532_F
GAAGGGTCAACAGTGAGATCGCCTGGTTC
>scaffold00003_48_49_741_R
TGTAGCTGCCCTTCTCCTCCTGTTC
>scaffold00003_49_50_208_F
GTGGTGTGCATCGTGCAGATCCCGTTCTAC
>scaffold00003_49_50_975_R
AACCCGGAGCCGAAGAGTTCGAGAAAC
>scaffold00003_50_51_1445_F
TGGCGTACCTGGAGAAGGTGGTTTC
>scaffold00003_50_51_439_R
CAATGGCACCCTCACATATGGCAGATAATTG
>scaffold00004_1_2_381_F
GAACACGTCACAACCGTGCTTCCAG
>scaffold00004_1_2_1879_R
ATCGTCTGGGTCAACACCTGGTTCCTG
>scaffold00004_2_3_250_F
GTTGAACCGTCTGAACGACCGAGGAAG
>scaffold00004_2_3_411_R
TGTTGTCGGGGTAGGTGGGTAAACG
>scaffold00004_3_4_333_F
TGACTTCTCCGACCAGATCGCACACCTGTC
>scaffold00004_3_4_675_R
GAGCTTCTCCTTGTGCTGGGTCAAC
>scaffold00004_4_5_297_F
GGGAGGTTGTAGATCACGAACTGGATGTAG
>scaffold00004_4_5_180_R
CAGAACGAGTCCTACAACGGGAACCTTTC
>scaffold00004_5_6_411_F
GAGATCATGTCGCTGGGCACCAATC
>scaffold00004_5_6_469_R
AGCAGAGCACCCGTCTGATGTACAG
>scaffold00004_6_7_257_F
CGCGTTGAAAAACCGGGCGATATCC
>scaffold00004_6_7_545_R
GTCTTCGTGGTCAACAAGGCCGATC
>scaffold00004_7_8_584_F
TGATCAACGAGGCGTGCAACGGTAG
>scaffold00004_7_8_419_R
ATGATCGACACCGTTACGGCGAGATGG
>scaffold00004_8_9_447_F
GAACCTGCTGTTCATGGTCGGTCTG
>scaffold00004_8_9_234_R
ACAGTGGTCTCCGTCTACAGTCCAC
>scaffold00004_9_10_1726_F
TAGAGGGTCAACGGTTCGTCACCAG
>scaffold00004_9_10_313_R
GTCAAGGATGACTGCGTGACTGGCTAC
>scaffold00004_10_11_783_F
AGAGCAGTTCGAGGATGTTGGGGTC
>scaffold00004_10_11_256_R
GCCGACTTCTACGTACTGCTGTCCATG
>scaffold00004_11_12_1607_F
TACCGACAAGTGAGAGGACGACGTG
>scaffold00004_11_12_704_R
GACCTGATCGCGGTGATTGGTTGAC
>scaffold00004_12_13_750_F
TCGATGGACATGCTCTCCTCCGAGATCGTC
>scaffold00004_12_13_895_R
TATGCGAGCGTCCGAAAAAGATCGACCAGC
>scaffold00004_13_14_550_F
GAGCACATCAAGATCACTGACAATGGGATC
>scaffold00004_13_14_243_R
AACACTCCGAAATCCGCTGGCTCTC
>scaffold00004_14_15_556_F
CTGGTGGGACTCGTTGAGCATCCAATG
>scaffold00004_14_15_220_R
CCGAGGGAAAGAACCGGACATTGTG
>scaffold00004_15_16_1515_F
CTTCACCGGCCTGTTCTTCGTCATCAC
>scaffold00004_15_16_167_R
CAGTACCTGTGGCGTCACACGCGATAAGAG
>scaffold00004_16_17_236_F
GGTTCGACACCAGGATTTCGAGGTCAC
>scaffold00004_16_17_467_R
GATCTGGTTCCACATCTGTCCCTGGTG
>scaffold00004_17_18_420_F
CCTGAGGCACAAGTCAGGGTATCGATC
>scaffold00004_17_18_720_R
CCAACATCAAGAGCCACCACAACGTC
>scaffold00004_18_19_226_F
GTGTGATCGACACGGTTACGTCGAAG
>scaffold00004_18_19_492_R
TTAACAGGGGGCCCTTCCTTCAACACGGTC
>scaffold00004_19_20_273_F
GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC
>scaffold00004_19_20_878_R
GACGAGCAACTGGACTCGCTGAACAAG
>scaffold00004_20_21_2075_F
AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
>scaffold00004_20_21_521_R
TGAAGATCACCCAGGATCTCAAGGAGTACG
>scaffold00004_21_22_386_F
GAAGACTTCGCCACCGAATACCTCAGC
>scaffold00004_21_22_182_R
TTCGACGTACACACCACCCTCGCCGTAATC
>scaffold00004_22_23_594_F
AATTCAGCGGACAGCGGTACGAGGGTGTAG
>scaffold00004_22_23_1225_R
AACAGGACCTGCTCGACAACAAGATCAAG
>scaffold00004_23_24_720_F
TCCACCGGAACATGGGTGATACTCGAC
>scaffold00004_23_24_552_R
GTTGCCTTTGCACAGGTAGATCCCG
>scaffold00004_24_25_667_F
CAAGCCATGTCGAAGCAGCACTACTC
>scaffold00004_24_25_322_R
AAGCACCTGCTGTTCACAAGCGAACTGATC
>scaffold00004_25_26_542_F
GCAGATCGACGAGTACCTGGAGTTCTAC
>scaffold00004_25_26_247_R
AGGAGTCCGACCATGAAGATCAGGAAGATC
>scaffold00004_26_27_255_F
GATCGTTCGACTACCCACAATCCGCAGATC
>scaffold00004_26_27_671_R
GGTAGAGCAGCCATTCCAGTTCCTGTG
>scaffold00004_27_28_577_F
CTGTTCACCGAGGAGGGCGAGTTCTTCTTC
>scaffold00004_27_28_485_R
GAAAACGGGGTGTTCCAGCTTCATCGCTTC
>scaffold00004_28_29_1409_F
AACCGACACACCTGCAGTTGCCGTACCAAC
>scaffold00004_28_29_207_R
TACTCCGGGATAAGCAAGGCTGAGCTG
>scaffold00004_29_30_278_F
CATTTGTGACACCTGTCCAGCAGTACG
>scaffold00004_29_30_264_R
TCTGGTGAGCCGACAGGTATTCCTC
>scaffold00004_30_31_444_F
ACCTTCTTGCTCTTACGGCCCACCTTG
>scaffold00004_30_31_739_R
GAGTACGTCAAGTTCATTTTCGCCACCAC
Retrieved from "http://compbio.korea.ac.kr/wiki/index.php/Primer_Verrucosispora_gap"
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