Primer Verrucosispora gap

From CSBLwiki

Revision as of 10:40, 19 February 2011 (view source) Igchoi (Talk | contribs) (→primers of scaffold & sole contigs) ← Older edit		Revision as of 10:41, 19 February 2011 (view source) Igchoi (Talk | contribs) (→Primer Naming Format) Newer edit →
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	==Primer Naming Format==		==Primer Naming Format==
-	===Scf&SolCtg primer format===	+	<!-- ===Scf&SolCtg primer format=== -->
	===Gap primer format===		===Gap primer format===
	*>[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse]		*>[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse]

---

Revision as of 10:41, 19 February 2011

Contents 1 Primer Naming Format 1.1 Gap primer format 2 List of primer 2.1 primers(intergenic) 2.2 primers(intragenic)

Primer Naming Format

Gap primer format

• >[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse]

ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R

                    CONTIG 1                                                     CONTIG 2
                               ------>
        5'                           3'  
--------CTCAGGTCAGGCAGGCGTGATGATCTCTTC----------------NNNNNNNNN----------------------------------------
                                                                       GTTAGAGGAGAGTGCGGCTGGGCTCCAATCAT
                                                                       3'                             5'
                                                                       <-------

                                            1105bp               529bp

List of primer

• By Using Primer3
• "Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer).

primers(intergenic)

• 72 gaps, 144 primers

>scaffold00001_2_3_751_F
CAGAACTGGAGAACGTCCATGCTCGAGAAC
>scaffold00001_2_3_1360_R
TCGATCACCAGCTCACGGAAGTCCTCGTTG
>scaffold00001_3_4_586_F
GTCAGTGCCGATCAGGATGTTGTCCAC
>scaffold00001_3_4_183_R
ATCAACTGCCAGAACTCGTCGGTCCTCATG
>scaffold00001_4_5_568_F
TACGTCGAGGAACTGGGCGACTTCAAGACC
>scaffold00001_4_5_838_R
CATGTCGTCGATCTGCACGTTGACCAG
>scaffold00001_9_10_1135_F
AGACCGGATTGAGTGCTTCCTCTGCTGGTG
>scaffold00001_9_10_1408_R
CGACCAATTTCCGACGAGGTGACATTC
>scaffold00001_11_12_354_F
AAGGTCAGGATGGCGGTGAACACCTTGGTC
>scaffold00001_11_12_345_R
ATGTTGTCGCTGACCTTCGCGATACGGGAG
>scaffold00001_12_13_1313_F
TTTCAGGCTTTGAGGATCGAGGTAAACAGG
>scaffold00001_12_13_315_R
TCGCATCTTCTCAGCCAGTTCCGAG
>scaffold00001_15_16_126613_F
GTTCCCGGTTGCGATCGGTGAGGAT
>scaffold00001_15_16_569_R
CTTCTACCACGACACACACACCGATCC
>scaffold00001_17_18_225_F
GATCGTGACGAACGTGAAGGTCCAC
>scaffold00001_17_18_408_R
CAGATCCGGTACAGCTCCTTGTATTCCAG
>scaffold00001_18_19_320_F
CAGATAGATGGTGAAGTCAGGACGACGG
>scaffold00001_18_19_177_R
ATGAACTTTCCACCGATGCCGGTCGTCTTC
>scaffold00001_21_22_243_F
AAGGACGGACGTGGACATCGATCAGCAATC
>scaffold00001_21_22_1379_R
AGGACTCCTGTACGTCGATCACGACGAGTG
>scaffold00001_23_24_410_F
ACTGACCACGGTGCAGCTCTACTTCAC
>scaffold00001_23_24_1184_R
AGCGTGTAGTCGGCGGTATACACCTTC
>scaffold00001_25_26_203_F
GTCGAGGGGATCAACACAAGTGGAATCAAG
>scaffold00001_25_26_184_R
GTACCGTCGACAAGCAACTCCTGATCGTC
>scaffold00001_27_28_280_F
TGACGTGAGGGGACTACGGATGTAC
>scaffold00001_27_28_260_R
ACTTCGACAGGTAGGCCTTGCAGAG
>scaffold00001_31_32_566_F
AAACGAGCCGTACAACGGGCAACTG
>scaffold00001_31_32_169_R
ATCTTCCTGACCTCGTCGGTGTGGTACTTC
>scaffold00001_34_35_1071_F
CAGTACGTCATCTTCGACCGGACCATTTG
>scaffold00001_34_35_2096_R
AGTCGTCGTCGTCGAACATCGTGAC
>scaffold00001_35_36_685_F
GTCGTTCAGGTTCAGGTAGAACTCGGTGAC
>scaffold00001_35_36_0_R
CCCGACCGAACGGCACGCACACAA
>scaffold00001_36_37_1073_F
GATCGGTTTGCCGCGGTCGTTGTATTCGAC
>scaffold00001_36_37_740_R
ATACGGCTTCGTCGAGAACCTGACC
>scaffold00001_38_39_612_F
AACATGTCGTTGAGCGCGGCCAGTTTG
>scaffold00001_38_39_672_R
CTCTTCGACGAGATCATCCAGGTGCTG
>scaffold00001_41_42_205_F
ACCGAAGCAGTCGAAGACGGTACAC
>scaffold00001_41_42_339_R
GTCTTCTTCTGGCCCCGCAACGAGATCATG
>scaffold00001_42_43_389_F
TAAAATCTGTGATCTGGGCTAACCTTGGGG
>scaffold00001_42_43_223_R
GTGATCGGTGTGTTCGTGGCTGACCAGTAC
>scaffold00001_45_46_401_F
GGATCAGCTCCGGATAGTTGAAGTCGTC
>scaffold00001_45_46_423_R
ACAACGAGGACTTCGACAACGGCTTCAAGG
>scaffold00001_47_48_1970_F
GACGACTACCTGCGTAAACCCTTCGACCTG
>scaffold00001_47_48_1044_R
CACCAATCTGCTTGTGGGTCTGGTGCTGAC
>scaffold00001_48_49_1639_F
GATGTTCATCGACCTGGAGAACGTGC
>scaffold00001_48_49_244_R
CATCTCTCCCGTCAGCTCATCGTTCGAAG
>scaffold00001_49_50_1297_F
CAGGGTTTCCTGAGCCACCTTGAAC
>scaffold00001_49_50_822_R
CTGTTCTCCCAGGACCCGATCTTCAACTC
>scaffold00001_50_51_227_F
CGTGCCATGTTCTGAACGAGAACGATC
>scaffold00001_50_51_860_R
GTGACCAGCGAGTGAATCTTGTCGCGTTG
>scaffold00001_55_56_239_F
CACCTGACCTCTGACAATGAACGCCGAAAG
>scaffold00001_55_56_206_R
GATTGGCTTCTATACAAACTAAGGTGGCGC
>scaffold00001_57_58_1204_F
TCATGCTGTCCGTATCCATTTAGGACCTTC
>scaffold00001_57_58_939_R
TTCTACCGTGCCTACTTCGAGACCGAC
>scaffold00001_61_62_285_F
TCAGGTTGAGATCGACCATCGCGAG
>scaffold00001_61_62_944_R
TTGCACCATCAGCTCAGCATCGTCTTCGAC
>scaffold00001_62_63_898_F
CTGATGATCAAGGTGTTGCGGCTGTC
>scaffold00001_62_63_367_R
GTTGTAGCCGCCGAAGTCGTTCATGCTGAC
>scaffold00001_67_68_5122_F
GACCGTTGCGACCAGATATTCGGCGATT
>scaffold00001_67_68_0_R
AACCGCTGACCGCGTTCGTGCTGTTCT
>scaffold00001_71_72_349_F
AACCCACGCATCCCACTCAACGAGCTGAAC
>scaffold00001_71_72_0_R
TCGTTGTCCACCACGCCGACGAACA
>scaffold00001_73_74_2968_F
TACGCGGCAGTCGGACGGGTGTGTT
>scaffold00001_73_74_0_R
GCTCACCGTGCCGGTGAGGACCAA
>scaffold00001_75_76_788_F
GTCGACGTTTCATCACGTCACTGGGTG
>scaffold00001_75_76_0_R
GTGCGCCAAGACCTGCGCGAACAGT
>scaffold00001_76_77_1854_F
GTGATCAAGGTGATCGAAGCCCTGC
>scaffold00001_76_77_535_R
ATCTACAACCACCTGGGCGGCAAGGAGAAC
>scaffold00001_80_81_426_F
CACTGCCCAAACCGATCTATCCGACGTTC
>scaffold00001_80_81_202_R
GATCCAAGCTGTCTTAACGAGGAGTTGCC
>scaffold00001_87_88_204_F
GAAGTGATCACGCGATCCACGGGATTCCAC
>scaffold00001_87_88_267_R
AGCTTCCAGACCCTGGTCAACAGTC
>scaffold00001_88_89_1973_F
AGTAGCCGATGACCCCGTTGAGTTG
>scaffold00001_88_89_463_R
CAGGTGTTCAACCTCGCCCAGACCTTCTAC
>scaffold00003_3_4_218_F
ACAAGCTCATTGCCTACTAGATCGACTTCG
>scaffold00003_3_4_1054_R
GCATCGTCCAACGGTAGCCTTCTTTC
>scaffold00003_7_8_365_F
ATCCGACGAGTACGACATCCACCTG
>scaffold00003_7_8_601_R
CAGACCAACTACGCCACAAGCAAGG
>scaffold00003_8_9_472_F
CTGTTCCAGACCTTCCTCACCGTGCTTGAC
>scaffold00003_8_9_218_R
CTTATGCCCTGACAGAAGGTGCAAGATCAG
>scaffold00003_10_11_1436_F
TGGTCCTGCCGTACTTCCTGATCACCACTG
>scaffold00003_10_11_232_R
GATGGGACAGACTTCGTACACCGGTTG
>scaffold00003_11_12_266_F
CTGTATTGATGATCCACTCGCTGCCGCTC
>scaffold00003_11_12_1184_R
TACAGTGATCGGCCGTTGTTGGAGTGTGTG
>scaffold00003_17_18_314_F
TCTGAACGAGACGGAATGGGAGATCCTCAC
>scaffold00003_17_18_222_R
GACATCACCATCCAGGCAGAGAACATGCTG
>scaffold00003_19_20_655_F
GAAGGAACGGCAACTTTCGGTCACGGTAC
>scaffold00003_19_20_485_R
CAAGGAGACCGTCTACAAGATCTGGCATC
>scaffold00003_23_24_235_F
CCCAGGGAATATAACGATGGTTCAGGTCAG
>scaffold00003_23_24_1163_R
GAACTGCTGGTTCATCGAATGGTCGTTGAC
>scaffold00003_24_25_268_F
CTCGAAGTCCCGGTCAAGAAGATCCTCAC
>scaffold00003_24_25_666_R
TATCGTTCGCGTCGATGGCCGACAACATTG
>scaffold00003_26_27_1270_F
GAAATCAAGATCCTGAACCAGTCCCGGAAG
>scaffold00003_26_27_244_R
CCCGATTCCTTGTCCTGGAACAGATAGAAG
>scaffold00003_27_28_833_F
GTACTCGCCGACTTTCCCGTTCTCATC
>scaffold00003_27_28_474_R
GTACACCTCGTGCTCGGGACACAAATGGTC
>scaffold00003_28_29_210_F
GTACACGTGCCGTTCCCGTTTCTGGTGAAC
>scaffold00003_28_29_173_R
ACGGCGTCACGAACAACACCTACTG
>scaffold00003_30_31_256_F
TTCCGGGGATGAGTAGCCGACCAATTGTCC
>scaffold00003_30_31_1239_R
GATCACCGGTTGGTAGACCAGATCCAG
>scaffold00003_31_32_351_F
CACAAATTGAACTTGAGCTGGGATGTCAGG
>scaffold00003_31_32_565_R
CAGGATGACAGTGTGAGTCAGCCATGGAC
>scaffold00003_35_36_707_F
AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
>scaffold00003_35_36_243_R
CAGGTTGAACATCTCCTGGACCAGCGAAC
>scaffold00003_42_43_317_F
GTTCCTGTCCTCGTGCAGCACAATTCGATC
>scaffold00003_42_43_298_R
ACCGGGGATCATCGTGGTCTTGTAC
>scaffold00003_43_44_737_F
ATCGTTCGCAGCGGCATCGATTCAC
>scaffold00003_43_44_992_R
TGAAGCTGACCAAGCTGGTGGTCTC
>scaffold00003_45_46_422_F
GTGATGCCCTCGGATCCCAGTTCTTCGTAC
>scaffold00003_45_46_399_R
AACTCGGTCAAGCTCGAGTTCCTGGTG
>scaffold00003_47_48_427_F
CATCACGGCGAACAACAACCAGACCTAC
>scaffold00003_47_48_271_R
GAACAACGGCATGAGGTCCTTGAACATGAC
>scaffold00003_48_49_532_F
GAAGGGTCAACAGTGAGATCGCCTGGTTC
>scaffold00003_48_49_741_R
TGTAGCTGCCCTTCTCCTCCTGTTC
>scaffold00003_49_50_208_F
GTGGTGTGCATCGTGCAGATCCCGTTCTAC
>scaffold00003_49_50_975_R
AACCCGGAGCCGAAGAGTTCGAGAAAC
>scaffold00003_50_51_1445_F
TGGCGTACCTGGAGAAGGTGGTTTC
>scaffold00003_50_51_439_R
CAATGGCACCCTCACATATGGCAGATAATTG
>scaffold00004_8_9_447_F
GAACCTGCTGTTCATGGTCGGTCTG
>scaffold00004_8_9_234_R
ACAGTGGTCTCCGTCTACAGTCCAC
>scaffold00004_12_13_750_F
TCGATGGACATGCTCTCCTCCGAGATCGTC
>scaffold00004_12_13_895_R
TATGCGAGCGTCCGAAAAAGATCGACCAGC
>scaffold00004_13_14_550_F
GAGCACATCAAGATCACTGACAATGGGATC
>scaffold00004_13_14_243_R
AACACTCCGAAATCCGCTGGCTCTC
>scaffold00004_14_15_556_F
CTGGTGGGACTCGTTGAGCATCCAATG
>scaffold00004_14_15_220_R
CCGAGGGAAAGAACCGGACATTGTG
>scaffold00004_15_16_1515_F
CTTCACCGGCCTGTTCTTCGTCATCAC
>scaffold00004_15_16_167_R
CAGTACCTGTGGCGTCACACGCGATAAGAG
>scaffold00004_16_17_236_F
GGTTCGACACCAGGATTTCGAGGTCAC
>scaffold00004_16_17_467_R
GATCTGGTTCCACATCTGTCCCTGGTG
>scaffold00004_18_19_226_F
GTGTGATCGACACGGTTACGTCGAAG
>scaffold00004_18_19_492_R
TTAACAGGGGGCCCTTCCTTCAACACGGTC
>scaffold00004_20_21_2075_F
AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
>scaffold00004_20_21_521_R
TGAAGATCACCCAGGATCTCAAGGAGTACG
>scaffold00004_21_22_386_F
GAAGACTTCGCCACCGAATACCTCAGC
>scaffold00004_21_22_182_R
TTCGACGTACACACCACCCTCGCCGTAATC
>scaffold00004_23_24_720_F
TCCACCGGAACATGGGTGATACTCGAC
>scaffold00004_23_24_552_R
GTTGCCTTTGCACAGGTAGATCCCG
>scaffold00004_24_25_667_F
CAAGCCATGTCGAAGCAGCACTACTC
>scaffold00004_24_25_322_R
AAGCACCTGCTGTTCACAAGCGAACTGATC
>scaffold00004_25_26_542_F
GCAGATCGACGAGTACCTGGAGTTCTAC
>scaffold00004_25_26_247_R
AGGAGTCCGACCATGAAGATCAGGAAGATC
>scaffold00004_29_30_278_F
CATTTGTGACACCTGTCCAGCAGTACG
>scaffold00004_29_30_264_R
TCTGGTGAGCCGACAGGTATTCCTC

primers(intragenic)

• 43 gaps, 86 primers

>scf1_10_11_191_R
ATTCCTCGCGCAGTTGACCCAGTTC
>scf1_10_11_533_F
TTCGCCACCACCGGTCTAGGATGAGAGATC
>scf1_13_14_1221_R
AGTCGTCGAAGCGCAGAAGGTACATGTAGG
>scf1_13_14_961_F
GTCATCGGACAGCCGATTCTTCGACGACTG
>scf1_14_15_1407_F
CTTGTTTCGATAGCATTACCGCCCAGTAAC
>scf1_14_15_533_R
GTACTCGTCGAAGGTGGACACGTTCACTC
>scf1_16_17_1785_F
CTCGAAGACGTCAACAACCACACCAATCTG
>scf1_16_17_478_R
CTCGTAACTCTCCATCGGTTCTCCAGTAAC
>scf1_22_23_238_F
GTTTCACTCATCGGTGTACCCTCCCGATC
>scf1_22_23_244_R
GAAGTTCGTCATCGAGAACGTCTCCGTGC
>scf1_24_25_444_F
ATTCGACGGGCAGGACGTCAACAAG
>scf1_24_25_458_R
TAGGTGTGCTCCAGGTAGGTGAGGAAC
>scf1_26_27_369_F
ATGAGCGAGCAGGTCTTCCTCCTAC
>scf1_26_27_706_R
GAACACGCCGTAGTTGTTCAGCGTGAAG
>scf1_29_30_1643_F
CCGATCCAGGCGAAGATCAGATTGGTC
>scf1_29_30_767_R
ATCGACATAGCGCTCCAACAGATCGGTGAC
>scf1_32_33_169_R
TTGTCAATGTAGCGTTGACGGGTGCCTGAC
>scf1_32_33_266_F
AACATTGACAGGGTGGCACGCAGTTCG
>scf1_33_34_365_R
GTTCCCTGCTGCTGAACTGGTTCTTCAC
>scf1_33_34_795_F
GTCGACGACGAGAATCCGGTTCATG
>scf1_37_38_498_F
TTCGACGAACTGTGGCAACGCTTCTCC
>scf1_37_38_562_R
AGGACGTCATGCACGGTCAACACAC
>scf1_40_41_522_F
GACTACAAGCCGGAGAACGTGCTCATCTC
>scf1_40_41_660_R
GTGCAGAAGGGATAGAGGGTGACGTAC
>scf1_43_44_440_F
CTCGGAGGAAAACGGTGAACAGTCAGCAAC
>scf1_43_44_563_R
TGAACGCCTCCACCCAGCATTTGATCACAG
>scf1_44_45_485_R
GTGTTGCGATCGGATTCGTAGCTGAC
>scf1_44_45_93801_F
ACACTGCTGCCGCCACGGTTGACTGT
>scf1_46_47_370_F
GTAAACTCCTGATCTCAGCCGCTCTCGTG
>scf1_46_47_845_R
CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG
>scf1_53_54_414_F
TTCTGGAAGACCATCTGCAGACCGATCTCG
>scf1_53_54_823_R
TACGCGAAGGGACGTTAGAGATGGCAC
>scf1_54_55_1250_F
CTGGAACCGGATGATGTTGGAGTCCTC
>scf1_54_55_175_R
TACCGAGGAACTGATCGACCTCTCC
>scf1_56_57_184_R
CAAACCGGTGGTCGGGCTGATCATGTATTC
>scf1_56_57_412_F
GTACTCACGATGGTCCTTCTCCTCGTTG
>scf1_59_60_1979_F
GTGACCAGCCAGGTCTGTTCGAATCTCAC
>scf1_59_60_625_R
GAGATCATGTACGCCGACTTCAGCTTCGTG
>scf1_63_64_1286_R
CGATAGGTGAGACTCGATGCATGGTTAGTG
>scf1_63_64_711_F
ACGTAGTTCCCGACCGTAGAGCAGTTC
>scf1_66_67_346_R
ACTGTTCCTGCTGTACTCCTCGGTG
>scf1_66_67_428_F
CTTCGTCACTGGCTGTGTCAAGGTCAAC
>scf1_6_7_870_R
CGATCTCCACGTTTGACAGGATGCTCTG
>scf1_6_7_939_F
ATCAGCGGTTACGTCTGGTGGTGAC
>scf1_7_8_545_R
ATCAGTTTGATCGGCGAGACGTGCGAGACC
>scf1_7_8_818_F
GAACTGGAGTTGATGGAGCAGCGCGAAAC
>scf3_12_13_2070_F
CTCCAGCAGGGTTTCATCGATTCGGTAG
>scf3_12_13_324_R
CTGGACCAGTTCACCCTCCAGATCATG
>scf3_18_19_471_R
GTATACCGCAACATCGGCGAAATGAAGTC
>scf3_18_19_746_F
GTTGCTTCGAATCTGGTGACGGGAG
>scf3_20_21_200_R
GAAGATCGGCAGCTCCATCGTCTCATC
>scf3_20_21_294_F
ATCAGCCCCGAGCAATTGACCGACCTGAAC
>scf3_21_22_1034_F
GAAGTAACCGGTGGTGTTTTCGGCCAG
>scf3_21_22_354_R
ATCTCGCCGGTTACAAGCAGATCCTC
>scf3_22_23_1299_F
TTCAGATGGTTAAGGACAGCTTCATGAGCG
>scf3_22_23_368_R
ACGTGATCGGTGGGAACTTCGACTC
>scf3_25_26_360_F
GAAAGGCTGACCATCTACCCGGAGTAC
>scf3_25_26_636_R
TGATCCACGTGTCCGTACATCATCGTG
>scf3_33_34_1969_F
GTCCTCAAGCATCGCAAGCGCAAGGAAAAG
>scf3_33_34_485_R
CTCCAACTGCTCGGAGAGGAACTTGTAAC
>scf3_34_35_222_R
ATCTCCGCAGTCATATCGGTACGGG
>scf3_34_35_491_F
GAACGAGACCAGGAGACATAGTGGCGAGTC
>scf3_36_37_355_F
CTGCTCTTCCTCGGCATGCTCATGTTC
>scf3_36_37_430_R
ATAGCCCGCCCACTGCATGACAAAC
>scf3_38_39_613_R
GTACGAGCAGTTCACCAGTTGCGTG
>scf3_38_39_791_F
AACTGTCGACGAAGAACCGCGTCATGAGAG
>scf3_39_40_333_R
CTGATCCGTCATGGCGCACATTCCACGATG
>scf3_39_40_351_F
CACTTGTCTTCTCCTCCGGATACCTGG
>scf3_44_45_1117_F
CTCTGTGAGTACGTGGAACAGGCGATG
>scf3_44_45_224_R
AAGATCACCACGGCGTCGACACAGCTCAAC
>scf3_6_7_245_R
GCATGCTGTTGTTGATGCTGTCCTTCAG
>scf3_6_7_918_F
ATGCTCAGTCCGGGAGTACAGCTCGGTAAC
>scf3_9_10_212_R
TTTCCGTTGAGCCGCCACTCGTACTTGTAG
>scf3_9_10_376_F
CATGCACAAGCAGGACCTCATCGAG
>scf4_19_20_273_F
GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC
>scf4_19_20_878_R
GACGAGCAACTGGACTCGCTGAACAAG
>scf4_26_27_255_F
GATCGTTCGACTACCCACAATCCGCAGATC
>scf4_26_27_671_R
GGTAGAGCAGCCATTCCAGTTCCTGTG
>scf4_27_28_485_R
GAAAACGGGGTGTTCCAGCTTCATCGCTTC
>scf4_27_28_577_F
CTGTTCACCGAGGAGGGCGAGTTCTTCTTC
>scf4_28_29_1409_F
AACCGACACACCTGCAGTTGCCGTACCAAC
>scf4_28_29_207_R
TACTCCGGGATAAGCAAGGCTGAGCTG
>scf4_30_31_444_F
ACCTTCTTGCTCTTACGGCCCACCTTG
>scf4_30_31_739_R
GAGTACGTCAAGTTCATTTTCGCCACCAC
>scf3_1_2_276_F
GACGTGTACATCTCGAACATCTCGCGG
>scf3_1_2_387_R
AAAACGGGCGTTCACCGCATGGTAC