Primer Verrucosispora gap

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==Primer Naming Format==
==Primer Naming Format==
-
*>[scaffold #]_[Contig_front #]_[Contig_back #]_[primer postion from N(bp)]
+
<!-- ===Scf&SolCtg primer format=== -->
-
ex) >scaffold00001_1_2_1105
+
===Gap primer format===
 +
*>[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse]
 +
<pre>
 +
ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R
   
   
-
         5'         contig1          3'                                           contig2
+
                    CONTIG 1                                                    CONTIG 2
-
  --------CTCAGGTCAGGCAGGCGTGATGATCTCTTC----------------NNNNNNNNN--------------------------------------
+
                                ------>
-
                                                                         TACTAACCTCGGGTCGGCGTGAGAGGAGATTG
+
         5'                           3'
 +
  --------CTCAGGTCAGGCAGGCGTGATGATCTCTTC----------------NNNNNNNNN----------------------------------------
 +
                                                                         GTTAGAGGAGAGTGCGGCTGGGCTCCAATCAT
                                                                         3'                            5'
                                                                         3'                            5'
-
                                            1105bp              529bp
+
                                                                        <-------
-
           
+
 +
Positions:                      1105bp                                      529bp
 +
</pre>
 +
==List of primer==
 +
<!--
 +
===primers of scaffold & sole contigs===
 +
====scf1====
 +
>scaffold00001_223_H
 +
TTGCGTGGGTTGAAGCAGAGCAAAGCAGTT
 +
>scaffold00001_2007_T
 +
ATGACCGCCAGGTGACTCTGCAACCCACTC
 +
====scf2====
 +
====scf3====
 +
>scaffold00003_1985_H
 +
CCGATCTCGTCATGTCCAGCCTGGATGAGT
 +
>scaffold00003_1004_T
 +
CCAAGTCCGACGGCTCGAAGTTCGGCAAGA
-
  >scaffold00001_1_2_1105
+
====scf4====
-
  CTCAGGTCAGGCAGGCGTGATGATCTCTTC
+
  >scaffold00004_151_H
-
  >scaffold00001_1_2_529
+
  GTTGATCTGCTGAGCGTGATTCGGATAGTT
-
  TACTAACCTCGGGTCGGCGTGAGAGGAGATTG
+
  >scaffold00004_548_T
-
  >scaffold00001_2_3_751
+
  CAACATCACCTCGCCGAAGACCGTCTATCT
 +
 
 +
====ctg205====
 +
*23s, 5s
 +
>23s5s_H
 +
TGGTGTGGTGTTGTTTGATATGTTGATAGG
 +
>23s5s_T
 +
ACGTCTCAGCCTACATGTGCCACGGATTTG
 +
 
 +
====ctg231====
 +
*16s
 +
>16s_H
 +
CGTGTTACTCACCCGTTCGCCGCTCGAGTA
 +
>16s_T
 +
TGGCGATTGGGACGAAGTCGTAACAAGGTA
 +
 
 +
-->
 +
 
 +
*By Using Primer3
 +
*"Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer).
 +
 
 +
===primers===
 +
*115 gaps (230 primers)
 +
 
 +
<pre>
 +
  >scaffold00001_2_3_751_F
  CAGAACTGGAGAACGTCCATGCTCGAGAAC
  CAGAACTGGAGAACGTCCATGCTCGAGAAC
-
  >scaffold00001_2_3_1360
+
  >scaffold00001_2_3_1360_R
  TCGATCACCAGCTCACGGAAGTCCTCGTTG
  TCGATCACCAGCTCACGGAAGTCCTCGTTG
-
  >scaffold00001_3_4_586
+
 
 +
  >scaffold00001_3_4_586_F
  GTCAGTGCCGATCAGGATGTTGTCCAC
  GTCAGTGCCGATCAGGATGTTGTCCAC
-
  >scaffold00001_3_4_183
+
  >scaffold00001_3_4_183_R
  ATCAACTGCCAGAACTCGTCGGTCCTCATG
  ATCAACTGCCAGAACTCGTCGGTCCTCATG
-
  >scaffold00001_4_5_568
+
 
 +
  >scaffold00001_4_5_568_F
  TACGTCGAGGAACTGGGCGACTTCAAGACC
  TACGTCGAGGAACTGGGCGACTTCAAGACC
-
  >scaffold00001_4_5_831
+
  >scaffold00001_4_5_838_R
-
  GATCTGCACGTTGACCAGGTGGTTG
+
  CATGTCGTCGATCTGCACGTTGACCAG
-
>scaffold00001_5_6_1090
+
 
-
GTTGAAGAAGCTGACCAGTCCGACGAGTAC
+
  >scaffold00001_9_10_1135_F
-
>scaffold00001_5_6_374
+
-
CGGGTCTTCTTCGAGATGGACATCTACTG
+
-
>scaffold00001_6_7_939
+
-
ATCAGCGGTTACGTCTGGTGGTGAC
+
-
>scaffold00001_6_7_870
+
-
CGATCTCCACGTTTGACAGGATGCTCTG
+
-
>scaffold00001_7_8_872
+
-
CTGCAACACGAACTGGTCTCGCTCAAC
+
-
>scaffold00001_7_8_546
+
-
CATCAGTTTGATCGGCGAGACGTGCGAGAC
+
-
>scaffold00001_8_9_527
+
-
CTGAGTAATCTTGGCCGCTGTGACAACC
+
-
>scaffold00001_8_9_358
+
-
ATGGACATGGTCCACCTGGTCATCG
+
-
  >scaffold00001_9_10_1135
+
  AGACCGGATTGAGTGCTTCCTCTGCTGGTG
  AGACCGGATTGAGTGCTTCCTCTGCTGGTG
-
  >scaffold00001_9_10_1408
+
  >scaffold00001_9_10_1408_R
  CGACCAATTTCCGACGAGGTGACATTC
  CGACCAATTTCCGACGAGGTGACATTC
-
>scaffold00001_10_11_533
+
 
-
TTCGCCACCACCGGTCTAGGATGAGAGATC
+
  >scaffold00001_11_12_354_F
-
>scaffold00001_10_11_191
+
-
ATTCCTCGCGCAGTTGACCCAGTTC
+
-
  >scaffold00001_11_12_354
+
  AAGGTCAGGATGGCGGTGAACACCTTGGTC
  AAGGTCAGGATGGCGGTGAACACCTTGGTC
-
  >scaffold00001_11_12_350
+
  >scaffold00001_11_12_345_R
-
  CATGTTGTCGCTGACCTTCGCGATAC
+
  ATGTTGTCGCTGACCTTCGCGATACGGGAG
-
  >scaffold00001_12_13_1313
+
 
 +
  >scaffold00001_12_13_1313_F
  TTTCAGGCTTTGAGGATCGAGGTAAACAGG
  TTTCAGGCTTTGAGGATCGAGGTAAACAGG
-
  >scaffold00001_12_13_315
+
  >scaffold00001_12_13_315_R
  TCGCATCTTCTCAGCCAGTTCCGAG
  TCGCATCTTCTCAGCCAGTTCCGAG
-
>scaffold00001_13_14_961
+
 
-
GTCATCGGACAGCCGATTCTTCGACGACTG
+
  >scaffold00001_15_16_126613_F
-
>scaffold00001_13_14_1221
+
  GTTCCCGGTTGCGATCGGTGAGGAT
-
AGTCGTCGAAGCGCAGAAGGTACATGTAGG
+
  >scaffold00001_15_16_569_R
-
>scaffold00001_14_15_1407
+
-
CTTGTTTCGATAGCATTACCGCCCAGTAAC
+
-
>scaffold00001_14_15_533
+
-
GTACTCGTCGAAGGTGGACACGTTCACTC
+
-
  >scaffold00001_15_16_126613
+
-
  Can't find proper primer
+
-
  >scaffold00001_15_16_569
+
  CTTCTACCACGACACACACACCGATCC
  CTTCTACCACGACACACACACCGATCC
-
>scaffold00001_16_17_1785
+
 
-
CTCGAAGACGTCAACAACCACACCAATCTG
+
  >scaffold00001_17_18_225_F
-
>scaffold00001_16_17_478
+
-
CTCGTAACTCTCCATCGGTTCTCCAGTAAC
+
-
  >scaffold00001_17_18_225
+
  GATCGTGACGAACGTGAAGGTCCAC
  GATCGTGACGAACGTGAAGGTCCAC
-
  >scaffold00001_17_18_408
+
  >scaffold00001_17_18_408_R
  CAGATCCGGTACAGCTCCTTGTATTCCAG
  CAGATCCGGTACAGCTCCTTGTATTCCAG
-
  >scaffold00001_18_19_320
+
 
 +
  >scaffold00001_18_19_320_F
  CAGATAGATGGTGAAGTCAGGACGACGG
  CAGATAGATGGTGAAGTCAGGACGACGG
-
  >scaffold00001_18_19_177
+
  >scaffold00001_18_19_177_R
  ATGAACTTTCCACCGATGCCGGTCGTCTTC
  ATGAACTTTCCACCGATGCCGGTCGTCTTC
-
>scaffold00001_19_20_313
+
 
-
GTGTCGTTGTAGGCGATGAGGAGTTGGTGG
+
  >scaffold00001_21_22_243_F
-
>scaffold00001_19_20_627
+
-
CACTCCAACTGGAGCACGATCTTGACG
+
-
>scaffold00001_20_21_312
+
-
TGTCGTTGTAGGCGACGAGGAGCTGATCAC
+
-
>scaffold00001_20_21_227
+
-
TCACCTACACCGACTACACCCTCTG
+
-
  >scaffold00001_21_22_243
+
  AAGGACGGACGTGGACATCGATCAGCAATC
  AAGGACGGACGTGGACATCGATCAGCAATC
-
  >scaffold00001_21_22_1379
+
  >scaffold00001_21_22_1379_R
  AGGACTCCTGTACGTCGATCACGACGAGTG
  AGGACTCCTGTACGTCGATCACGACGAGTG
-
>scaffold00001_22_23_238
+
 
-
GTTTCACTCATCGGTGTACCCTCCCGATC
+
  >scaffold00001_23_24_410_F
-
>scaffold00001_22_23_248
+
-
ATCCCGAAGTTCGTCATCGAGAACGTCTCC
+
-
  >scaffold00001_23_24_410
+
  ACTGACCACGGTGCAGCTCTACTTCAC
  ACTGACCACGGTGCAGCTCTACTTCAC
-
  >scaffold00001_23_24_1184
+
  >scaffold00001_23_24_1184_R
  AGCGTGTAGTCGGCGGTATACACCTTC
  AGCGTGTAGTCGGCGGTATACACCTTC
-
>scaffold00001_24_25_444
+
 
-
ATTCGACGGGCAGGACGTCAACAAG
+
  >scaffold00001_25_26_203_F
-
>scaffold00001_24_25_279
+
  GTCGAGGGGATCAACACAAGTGGAATCAAG
-
GAAGTCGGAGTGGAACAGCTTGTAGGAGAC
+
  >scaffold00001_25_26_184_R
-
  >scaffold00001_25_26_206
+
-
  CTCGTCGAGGGGATCAACACAAGTGGAATC
+
-
  >scaffold00001_25_26_184
+
  GTACCGTCGACAAGCAACTCCTGATCGTC
  GTACCGTCGACAAGCAACTCCTGATCGTC
-
>scaffold00001_26_27_369
+
 
-
ATGAGCGAGCAGGTCTTCCTCCTAC
+
  >scaffold00001_27_28_280_F
-
>scaffold00001_26_27_706
+
-
GAACACGCCGTAGTTGTTCAGCGTGAAG
+
-
  >scaffold00001_27_28_280
+
  TGACGTGAGGGGACTACGGATGTAC
  TGACGTGAGGGGACTACGGATGTAC
-
  >scaffold00001_27_28_260
+
  >scaffold00001_27_28_260_R
  ACTTCGACAGGTAGGCCTTGCAGAG
  ACTTCGACAGGTAGGCCTTGCAGAG
-
>scaffold00001_28_29_209
+
 
-
TCTACGGTGGGTTGCACTGGTTTAGAGAAG
+
  >scaffold00001_31_32_566_F
-
>scaffold00001_28_29_357
+
-
TACAGCTGAAGGATCTTCTCGTCGAGGACC
+
-
>scaffold00001_29_30_1643
+
-
CCGATCCAGGCGAAGATCAGATTGGTC
+
-
>scaffold00001_29_30_767
+
-
ATCGACATAGCGCTCCAACAGATCGGTGAC
+
-
>scaffold00001_30_31_237
+
-
CACCACACGTTGTACGTCTTCGTGCTCTAC
+
-
>scaffold00001_30_31_284
+
-
CATCCCCCAAGTCTTTCCTCTCATGCTCTG
+
-
  >scaffold00001_31_32_566
+
  AAACGAGCCGTACAACGGGCAACTG
  AAACGAGCCGTACAACGGGCAACTG
-
  >scaffold00001_31_32_182
+
  >scaffold00001_31_32_169_R
-
  TGATCGGCATCTTCCTGACCTCGTC
+
  ATCTTCCTGACCTCGTCGGTGTGGTACTTC
-
>scaffold00001_32_33_311
+
 
-
ATTCTGAGTCGAGTGGAGGCAAGACTGCTG
+
  >scaffold00001_34_35_1071_F
-
>scaffold00001_32_33_182
+
  CAGTACGTCATCTTCGACCGGACCATTTG
-
GAAGGGTCCGGCGTTGTCAATGTAGCGTTG
+
  >scaffold00001_34_35_2096_R
-
>scaffold00001_33_34_795
+
-
GTCGACGACGAGAATCCGGTTCATG
+
-
>scaffold00001_33_34_365
+
-
GTTCCCTGCTGCTGAACTGGTTCTTCAC
+
-
  >scaffold00001_34_35_1062
+
-
  ATCTTCGACCGGACCATTTGGAGTCGGACC
+
-
  >scaffold00001_34_35_2096
+
  AGTCGTCGTCGTCGAACATCGTGAC
  AGTCGTCGTCGTCGAACATCGTGAC
-
  >scaffold00001_35_36_685
+
 
 +
  >scaffold00001_35_36_685_F
  GTCGTTCAGGTTCAGGTAGAACTCGGTGAC
  GTCGTTCAGGTTCAGGTAGAACTCGGTGAC
-
  >scaffold00001_35_36_0
+
  >scaffold00001_35_36_0_R
-
  Can't find proper primer
+
  CCCGACCGAACGGCACGCACACAA
-
  >scaffold00001_36_37_1058
+
 
-
  GTCGTTGTATTCGACCGGCGGTTTC
+
  >scaffold00001_36_37_1073_F
-
  >scaffold00001_36_37_740
+
  GATCGGTTTGCCGCGGTCGTTGTATTCGAC
 +
  >scaffold00001_36_37_740_R
  ATACGGCTTCGTCGAGAACCTGACC
  ATACGGCTTCGTCGAGAACCTGACC
-
>scaffold00001_37_38_498
+
 
-
TTCGACGAACTGTGGCAACGCTTCTCC
+
  >scaffold00001_38_39_612_F
-
>scaffold00001_37_38_562
+
  AACATGTCGTTGAGCGCGGCCAGTTTG
-
AGGACGTCATGCACGGTCAACACAC
+
  >scaffold00001_38_39_672_R
-
  >scaffold00001_38_39_625
+
-
  TTCCAGCAGGGCGAACATGTCGTTGAG
+
-
  >scaffold00001_38_39_672
+
  CTCTTCGACGAGATCATCCAGGTGCTG
  CTCTTCGACGAGATCATCCAGGTGCTG
-
>scaffold00001_39_40_203
+
 
-
GCCAGCCCACAATTTCATCGATCAACAC
+
  >scaffold00001_41_42_205_F
-
>scaffold00001_39_40_425
+
-
ACCTGCAACTGACTCGGCTGGCTATAGCTG
+
-
>scaffold00001_40_41_522
+
-
GACTACAAGCCGGAGAACGTGCTCATCTC
+
-
>scaffold00001_40_41_660
+
-
GTGCAGAAGGGATAGAGGGTGACGTAC
+
-
  >scaffold00001_41_42_205
+
  ACCGAAGCAGTCGAAGACGGTACAC
  ACCGAAGCAGTCGAAGACGGTACAC
-
  >scaffold00001_41_42_564
+
  >scaffold00001_41_42_339_R
-
  GTCATGCTCTACCCGAACATCTTCCACGAC
+
  GTCTTCTTCTGGCCCCGCAACGAGATCATG
-
  >scaffold00001_42_43_389
+
 
 +
  >scaffold00001_42_43_389_F
  TAAAATCTGTGATCTGGGCTAACCTTGGGG
  TAAAATCTGTGATCTGGGCTAACCTTGGGG
-
  >scaffold00001_42_43_223
+
  >scaffold00001_42_43_223_R
  GTGATCGGTGTGTTCGTGGCTGACCAGTAC
  GTGATCGGTGTGTTCGTGGCTGACCAGTAC
-
>scaffold00001_43_44_440
+
 
-
CTCGGAGGAAAACGGTGAACAGTCAGCAAC
+
  >scaffold00001_45_46_401_F
-
>scaffold00001_43_44_563
+
-
TGAACGCCTCCACCCAGCATTTGATCACAG
+
-
>scaffold00001_44_45_93801
+
-
Can't find proper primer
+
-
>scaffold00001_44_45_485
+
-
GTGTTGCGATCGGATTCGTAGCTGAC
+
-
  >scaffold00001_45_46_401
+
  GGATCAGCTCCGGATAGTTGAAGTCGTC
  GGATCAGCTCCGGATAGTTGAAGTCGTC
-
  >scaffold00001_45_46_423
+
  >scaffold00001_45_46_423_R
  ACAACGAGGACTTCGACAACGGCTTCAAGG
  ACAACGAGGACTTCGACAACGGCTTCAAGG
-
>scaffold00001_46_47_381
+
 
-
TAGTCACATGCGTAAACTCCTGATCTCAGC
+
  >scaffold00001_47_48_1970_F
-
>scaffold00001_46_47_845
+
-
CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG
+
-
  >scaffold00001_47_48_1970
+
  GACGACTACCTGCGTAAACCCTTCGACCTG
  GACGACTACCTGCGTAAACCCTTCGACCTG
-
  >scaffold00001_47_48_1052
+
  >scaffold00001_47_48_1044_R
-
  TACCTGCTCACCAATCTGCTTGTGGGTCTG
+
  CACCAATCTGCTTGTGGGTCTGGTGCTGAC
-
  >scaffold00001_48_49_1639
+
 
 +
  >scaffold00001_48_49_1639_F
  GATGTTCATCGACCTGGAGAACGTGC
  GATGTTCATCGACCTGGAGAACGTGC
-
  >scaffold00001_48_49_229
+
  >scaffold00001_48_49_244_R
-
  CTCATCGTTCGAAGCGAGGTAACGTCCTC
+
  CATCTCTCCCGTCAGCTCATCGTTCGAAG
-
  >scaffold00001_49_50_1297
+
 
 +
  >scaffold00001_49_50_1297_F
  CAGGGTTTCCTGAGCCACCTTGAAC
  CAGGGTTTCCTGAGCCACCTTGAAC
-
  >scaffold00001_49_50_822
+
  >scaffold00001_49_50_822_R
  CTGTTCTCCCAGGACCCGATCTTCAACTC
  CTGTTCTCCCAGGACCCGATCTTCAACTC
-
  >scaffold00001_50_51_227
+
 
 +
  >scaffold00001_50_51_227_F
  CGTGCCATGTTCTGAACGAGAACGATC
  CGTGCCATGTTCTGAACGAGAACGATC
-
  >scaffold00001_50_51_860
+
  >scaffold00001_50_51_860_R
  GTGACCAGCGAGTGAATCTTGTCGCGTTG
  GTGACCAGCGAGTGAATCTTGTCGCGTTG
-
>scaffold00001_51_52_1410
+
 
-
GATGTCCACGTCGTACGCCATGCTGTTGTC
+
  >scaffold00001_55_56_239_F
-
>scaffold00001_51_52_509
+
-
CCTGATTCACCTGATTCGTCCACCAGTTC
+
-
>scaffold00001_52_53_361
+
-
GTCATCCACACATGGCATTCTGTGACCG
+
-
>scaffold00001_52_53_353
+
-
AGGATGGCGTAATCCGCGATGATGG
+
-
>scaffold00001_53_54_414
+
-
TTCTGGAAGACCATCTGCAGACCGATCTCG
+
-
>scaffold00001_53_54_823
+
-
TACGCGAAGGGACGTTAGAGATGGCAC
+
-
>scaffold00001_54_55_1250
+
-
CTGGAACCGGATGATGTTGGAGTCCTC
+
-
>scaffold00001_54_55_176
+
-
GTACCGAGGAACTGATCGACCTCTC
+
-
  >scaffold00001_55_56_239
+
  CACCTGACCTCTGACAATGAACGCCGAAAG
  CACCTGACCTCTGACAATGAACGCCGAAAG
-
  >scaffold00001_55_56_206
+
  >scaffold00001_55_56_206_R
  GATTGGCTTCTATACAAACTAAGGTGGCGC
  GATTGGCTTCTATACAAACTAAGGTGGCGC
-
>scaffold00001_56_57_412
+
 
-
GTACTCACGATGGTCCTTCTCCTCGTTGC
+
  >scaffold00001_57_58_1204_F
-
>scaffold00001_56_57_184
+
-
CAAACCGGTGGTCGGGCTGATCATGTATTC
+
-
  >scaffold00001_57_58_1204
+
  TCATGCTGTCCGTATCCATTTAGGACCTTC
  TCATGCTGTCCGTATCCATTTAGGACCTTC
-
  >scaffold00001_57_58_939
+
  >scaffold00001_57_58_939_R
  TTCTACCGTGCCTACTTCGAGACCGAC
  TTCTACCGTGCCTACTTCGAGACCGAC
-
>scaffold00001_58_59_788
+
 
-
CAAAGCAAATCGGAGGGGACATGACG
+
  >scaffold00001_61_62_285_F
-
>scaffold00001_58_59_229
+
-
ACACGTTCGTCCGCAACAACGGATG
+
-
>scaffold00001_59_60_1979
+
-
GTGACCAGCCAGGTCTGTTCGAATCTCAC
+
-
>scaffold00001_59_60_581
+
-
GTCAACCAGATCGCCAACATCCGCTACTC
+
-
>scaffold00001_60_61_755
+
-
GTACGGTTTCTGACCAACACCGACTCGAAG
+
-
>scaffold00001_60_61_647
+
-
CCAACATCACCATCACGAACCTCGG
+
-
  >scaffold00001_61_62_285
+
  TCAGGTTGAGATCGACCATCGCGAG
  TCAGGTTGAGATCGACCATCGCGAG
-
  >scaffold00001_61_62_383
+
  >scaffold00001_61_62_944_R
-
  TTGGATGACCTCACGCTGACCTTCGGATTG
+
  TTGCACCATCAGCTCAGCATCGTCTTCGAC
-
  >scaffold00001_62_63_898
+
 
 +
  >scaffold00001_62_63_898_F
  CTGATGATCAAGGTGTTGCGGCTGTC
  CTGATGATCAAGGTGTTGCGGCTGTC
-
  >scaffold00001_62_63_367
+
  >scaffold00001_62_63_367_R
  GTTGTAGCCGCCGAAGTCGTTCATGCTGAC
  GTTGTAGCCGCCGAAGTCGTTCATGCTGAC
-
>scaffold00001_63_64_711
+
 
-
ACGTAGTTCCCGACCGTAGAGCAGTTC
+
  >scaffold00001_67_68_5122_F
-
  >scaffold00001_63_64_1270
+
  GACCGTTGCGACCAGATATTCGGCGATT
-
  GTGCATCAGCAGTGCAAAT
+
  >scaffold00001_67_68_0_R
-
  >scaffold00001_64_65_324
+
  AACCGCTGACCGCGTTCGTGCTGTTCT
-
  CTGGTGTGCTTTTCGGTTCGGGTAC
+
 
-
>scaffold00001_64_65_297
+
  >scaffold00001_71_72_349_F
-
AACGCCCACGTCATCCTCGAAGAAC
+
-
>scaffold00001_65_66_1986
+
-
GATCAGTCCGGCGATCGACGAGTAGAGAAC
+
-
>scaffold00001_65_66_1292
+
-
GTTGCTGCTGGAAGTGGCATGGGAAAC
+
-
>scaffold00001_66_67_428
+
-
CTTCGTCACTGGCTGTGTCAAGGTCAAC
+
-
>scaffold00001_66_67_346
+
-
ACTGTTCCTGCTGTACTCCTCGGTG
+
-
>scaffold00001_67_68_262
+
-
TGACCGTTGCGACCAGATATTCGGCGATTG
+
-
>scaffold00001_67_68_0
+
-
Can't find proper primer
+
-
>scaffold00001_68_69_5187
+
-
Can't find proper primer
+
-
>scaffold00001_68_69_0
+
-
Can't find proper primer
+
-
>scaffold00001_69_70_254
+
-
CTTCGACATGGAGGCAGTGCTTGAGAACTC
+
-
>scaffold00001_69_70_318
+
-
AGTGAGGAGGAACAGTTCTACAATGGTGTG
+
-
>scaffold00001_70_71_240
+
-
CATTCGGCGTGGTAAAGACCCACTGGTAC
+
-
>scaffold00001_70_71_233
+
-
ATCGATGACCCTCCAAGACAGACCTCATTG
+
-
  >scaffold00001_71_72_349
+
  AACCCACGCATCCCACTCAACGAGCTGAAC
  AACCCACGCATCCCACTCAACGAGCTGAAC
-
  >scaffold00001_71_72_0
+
  >scaffold00001_71_72_0_R
-
  Can't find proper primer
+
  TCGTTGTCCACCACGCCGACGAACA
-
>scaffold00001_72_73_2964
+
 
-
Can't find proper primer
+
  >scaffold00001_73_74_2968_F
-
  >scaffold00001_72_73_0
+
  TACGCGGCAGTCGGACGGGTGTGTT
-
  Can't find proper primer
+
  >scaffold00001_73_74_0_R
-
  >scaffold00001_73_74_2968
+
  GCTCACCGTGCCGGTGAGGACCAA
-
  Can't find proper primer
+
 
-
>scaffold00001_73_74_0
+
  >scaffold00001_75_76_788_F
-
Can't find proper primer
+
-
>scaffold00001_74_75_356
+
-
GTGTTCACCGGCGTGATGTACAACGACTAC
+
-
>scaffold00001_74_75_0
+
-
Can't find proper primer
+
-
  >scaffold00001_75_76_788
+
  GTCGACGTTTCATCACGTCACTGGGTG
  GTCGACGTTTCATCACGTCACTGGGTG
-
  >scaffold00001_75_76_0
+
  >scaffold00001_75_76_0_R
-
  Can't find proper primer
+
  GTGCGCCAAGACCTGCGCGAACAGT
-
  >scaffold00001_76_77_1854
+
 
 +
  >scaffold00001_76_77_1854_F
  GTGATCAAGGTGATCGAAGCCCTGC
  GTGATCAAGGTGATCGAAGCCCTGC
-
  >scaffold00001_76_77_535
+
  >scaffold00001_76_77_535_R
  ATCTACAACCACCTGGGCGGCAAGGAGAAC
  ATCTACAACCACCTGGGCGGCAAGGAGAAC
-
>scaffold00001_77_78_625
+
 
-
CAATCTCATGGGGTGCCTTCGTCGATC
+
  >scaffold00001_80_81_426_F
-
>scaffold00001_77_78_349
+
-
CTCGGCAACCGACTGTGGAACAACATC
+
-
>scaffold00001_78_79_237
+
-
CTACCGCTCAATACGCCAACGTCACATC
+
-
>scaffold00001_78_79_276
+
-
GAAATGCCGTCACTCTCCGTAGATGTCC
+
-
>scaffold00001_79_80_301
+
-
TACGTTTGGCTCAACACCCCACGCGAGATG
+
-
>scaffold00001_79_80_212
+
-
GTCGCTGATCATGGTTCGGTAGACGATGTC
+
-
  >scaffold00001_80_81_426
+
  CACTGCCCAAACCGATCTATCCGACGTTC
  CACTGCCCAAACCGATCTATCCGACGTTC
-
  >scaffold00001_80_81_202
+
  >scaffold00001_80_81_202_R
  GATCCAAGCTGTCTTAACGAGGAGTTGCC
  GATCCAAGCTGTCTTAACGAGGAGTTGCC
-
>scaffold00001_81_82_751
+
 
-
CAAATCCGGGCACCTCGAACTCAAG
+
  >scaffold00001_87_88_204_F
-
>scaffold00001_81_82_233
+
-
AACACCACCACCCCCGAGTAGCAGACAACAAC
+
-
>scaffold00001_82_83_253
+
-
CAAGGTCATCGTCAGCGGTGGATTG
+
-
>scaffold00001_82_83_302
+
-
AGATCCGGATACTGCGCTGGGAAAC
+
-
>scaffold00001_83_84_243
+
-
GTGGGGCAAGTGCTGGAGTTTGATTCAC
+
-
>scaffold00001_83_84_182
+
-
GTGGACGGACCAGTCGTGATGTTCGATTC
+
-
>scaffold00001_84_85_413
+
-
TTTACCCTCGATCCGGCAGATGAAGCCCTC
+
-
>scaffold00001_84_85_726
+
-
GACCCGTCCAACACCTTCGATCAGAGCTTC
+
-
>scaffold00001_85_86_207
+
-
GCACGGTTTAGGAAGTGTGGACACG
+
-
>scaffold00001_85_86_191
+
-
CCGAAGTGTGCAAACGTTGTCTTTACGTC
+
-
>scaffold00001_86_87_218
+
-
TCAACAACCCAGTGAACACCGAGGACGTAC
+
-
>scaffold00001_86_87_256
+
-
GTCGAGGCCATTGAGAAAGCGATGTAC
+
-
  >scaffold00001_87_88_204
+
  GAAGTGATCACGCGATCCACGGGATTCCAC
  GAAGTGATCACGCGATCCACGGGATTCCAC
-
  >scaffold00001_87_88_267
+
  >scaffold00001_87_88_267_R
  AGCTTCCAGACCCTGGTCAACAGTC
  AGCTTCCAGACCCTGGTCAACAGTC
-
  >scaffold00001_88_89_1973
+
 
 +
  >scaffold00001_88_89_1973_F
  AGTAGCCGATGACCCCGTTGAGTTG
  AGTAGCCGATGACCCCGTTGAGTTG
-
  >scaffold00001_88_89_463
+
  >scaffold00001_88_89_463_R
  CAGGTGTTCAACCTCGCCCAGACCTTCTAC
  CAGGTGTTCAACCTCGCCCAGACCTTCTAC
-
>scaffold00001_89_90_613
+
 
-
ATGACCTTCGTGAAACTGACCTTCTCGCTG
+
  >scaffold00003_3_4_218_F
-
>scaffold00001_89_90_528
+
-
GACAAGGACTCCGCCAAGAAGGACGAATC
+
-
>scaffold00003_1_2_276
+
-
GACGTGTACATCTCGAACATCTCGCGG
+
-
>scaffold00003_1_2_427
+
-
CCGTCCTACCTTCCTTTGTGGTTCCTTC
+
-
>scaffold00003_2_3_232
+
-
CAAGGGAGAGTTCCGTTACGACCACAG
+
-
>scaffold00003_2_3_460
+
-
TTGATCAGCCGGCACAGCAACAAGGTGGTG
+
-
  >scaffold00003_3_4_218
+
  ACAAGCTCATTGCCTACTAGATCGACTTCG
  ACAAGCTCATTGCCTACTAGATCGACTTCG
-
  >scaffold00003_3_4_981
+
  >scaffold00003_3_4_1054_R
-
  GAAAGACGAATCCCGTGAGAATCGTGGTG
+
  GCATCGTCCAACGGTAGCCTTCTTTC
-
>scaffold00003_4_5_574
+
 
-
ATCGACCTGGTGCACGCGAACAACTCCAAG
+
  >scaffold00003_7_8_365_F
-
>scaffold00003_4_5_289
+
-
GAAGAAGGCGTTGGAGTGCCACAGTTG
+
-
>scaffold00003_5_6_207
+
-
GAGCTTTCTGTGATCTGTTGCCGAATTGG
+
-
>scaffold00003_5_6_263
+
-
ATGGCATCCCTCAACCACTACCACC
+
-
>scaffold00003_6_7_918
+
-
ATGCTCAGTCCGGGAGTACAGCTCGGTAAC
+
-
>scaffold00003_6_7_220
+
-
TTCAGCCTGTAGGAAACCACGCCCGAACAG
+
-
  >scaffold00003_7_8_365
+
  ATCCGACGAGTACGACATCCACCTG
  ATCCGACGAGTACGACATCCACCTG
-
  >scaffold00003_7_8_601
+
  >scaffold00003_7_8_601_R
  CAGACCAACTACGCCACAAGCAAGG
  CAGACCAACTACGCCACAAGCAAGG
-
  >scaffold00003_8_9_472
+
 
 +
  >scaffold00003_8_9_472_F
  CTGTTCCAGACCTTCCTCACCGTGCTTGAC
  CTGTTCCAGACCTTCCTCACCGTGCTTGAC
-
  >scaffold00003_8_9_218
+
  >scaffold00003_8_9_218_R
  CTTATGCCCTGACAGAAGGTGCAAGATCAG
  CTTATGCCCTGACAGAAGGTGCAAGATCAG
-
>scaffold00003_9_10_376
+
 
-
CATGCACAAGCAGGACCTCATCGAG
+
  >scaffold00003_10_11_1436_F
-
>scaffold00003_9_10_212
+
-
TTTCCGTTGAGCCGCCACTCGTACTTGTAG
+
-
  >scaffold00003_10_11_1436
+
  TGGTCCTGCCGTACTTCCTGATCACCACTG
  TGGTCCTGCCGTACTTCCTGATCACCACTG
-
  >scaffold00003_10_11_232
+
  >scaffold00003_10_11_232_R
  GATGGGACAGACTTCGTACACCGGTTG
  GATGGGACAGACTTCGTACACCGGTTG
-
  >scaffold00003_11_12_266
+
 
 +
  >scaffold00003_11_12_266_F
  CTGTATTGATGATCCACTCGCTGCCGCTC
  CTGTATTGATGATCCACTCGCTGCCGCTC
-
  >scaffold00003_11_12_1184
+
  >scaffold00003_11_12_1184_R
  TACAGTGATCGGCCGTTGTTGGAGTGTGTG
  TACAGTGATCGGCCGTTGTTGGAGTGTGTG
-
>scaffold00003_12_13_2070
+
 
-
CTCCAGCAGGGTTTCATCGATTCGGTAG
+
  >scaffold00003_17_18_314_F
-
>scaffold00003_12_13_324
+
  TCTGAACGAGACGGAATGGGAGATCCTCAC
-
CTGGACCAGTTCACCCTCCAGATCATG
+
  >scaffold00003_17_18_222_R
-
>scaffold00003_13_14_463
+
-
CTGGTGTCACACAACGAAAAGGACCTGACC
+
-
>scaffold00003_13_14_304
+
-
GAAGAAGTCGTCCTTCTCCTTGACCGACAG
+
-
>scaffold00003_14_15_358
+
-
GCACACAACATCCAAGATCATTGTTGATCG
+
-
>scaffold00003_14_15_518
+
-
AATACCGCTACGGTGCTGGAACCGATGTAC
+
-
>scaffold00003_15_16_227
+
-
GTTTCTGGACAAGTACGGTTTCGGGACC
+
-
>scaffold00003_15_16_241
+
-
CTCACCTCGTCTCCCAAGGCGATCTGAAAC
+
-
>scaffold00003_16_17_285
+
-
CTTCCTACCACCGCCGTCCACAGTGTTTAG
+
-
>scaffold00003_16_17_503
+
-
TACCTGTGGCCCCAAAGCTACTACCTG
+
-
  >scaffold00003_17_18_313
+
-
  CTGAACGAGACGGAATGGGAGATCCTCACC
+
-
  >scaffold00003_17_18_222
+
  GACATCACCATCCAGGCAGAGAACATGCTG
  GACATCACCATCCAGGCAGAGAACATGCTG
-
>scaffold00003_18_19_746
+
 
-
GTTGCTTCGAATCTGGTGACGGGAG
+
  >scaffold00003_19_20_655_F
-
>scaffold00003_18_19_471
+
-
GTATACCGCAACATCGGCGAAATGAAGTC
+
-
  >scaffold00003_19_20_655
+
  GAAGGAACGGCAACTTTCGGTCACGGTAC
  GAAGGAACGGCAACTTTCGGTCACGGTAC
-
  >scaffold00003_19_20_485
+
  >scaffold00003_19_20_485_R
  CAAGGAGACCGTCTACAAGATCTGGCATC
  CAAGGAGACCGTCTACAAGATCTGGCATC
-
>scaffold00003_20_21_404
+
 
-
AGCTGTTCGACAACGCCACCGCATTCTCAC
+
  >scaffold00003_23_24_235_F
-
>scaffold00003_20_21_200
+
-
GAAGATCGGCAGCTCCATCGTCTCATC
+
-
>scaffold00003_21_22_1034
+
-
GAAGTAACCGGTGGTGTTTTCGGCCAG
+
-
>scaffold00003_21_22_354
+
-
ATCTCGCCGGTTACAAGCAGATCCTC
+
-
>scaffold00003_22_23_1299
+
-
TTCAGATGGTTAAGGACAGCTTCATGAGCG
+
-
>scaffold00003_22_23_368
+
-
ACGTGATCGGTGGGAACTTCGACTC
+
-
  >scaffold00003_23_24_235
+
  CCCAGGGAATATAACGATGGTTCAGGTCAG
  CCCAGGGAATATAACGATGGTTCAGGTCAG
-
  >scaffold00003_23_24_1082
+
  >scaffold00003_23_24_1163_R
-
  CACCACCCGCTCGTTGGTGAACAGATAGTG
+
  GAACTGCTGGTTCATCGAATGGTCGTTGAC
-
  >scaffold00003_24_25_274
+
 
-
  AAGAAGCTCGAAGTCCCGGTCAAGAAGATC
+
  >scaffold00003_24_25_268_F
-
  >scaffold00003_24_25_666
+
  CTCGAAGTCCCGGTCAAGAAGATCCTCAC
 +
  >scaffold00003_24_25_666_R
  TATCGTTCGCGTCGATGGCCGACAACATTG
  TATCGTTCGCGTCGATGGCCGACAACATTG
-
>scaffold00003_25_26_360
+
 
-
GAAAGGCTGACCATCTACCCGGAGTAC
+
  >scaffold00003_26_27_1270_F
-
  >scaffold00003_25_26_632
+
  GAAATCAAGATCCTGAACCAGTCCCGGAAG
-
  ATCCACGTGTCCGTACATCATCGTGGAGC
+
  >scaffold00003_26_27_244_R
-
  >scaffold00003_26_27_1276
+
  CCCGATTCCTTGTCCTGGAACAGATAGAAG
-
  ATCCTGGAAATCAAGATCCTGAACCAGTCC
+
 
-
>scaffold00003_26_27_235
+
  >scaffold00003_27_28_833_F
-
TTGTCCTGGAACAGATAGAAGTCGTGGCCG
+
-
  >scaffold00003_27_28_833
+
  GTACTCGCCGACTTTCCCGTTCTCATC
  GTACTCGCCGACTTTCCCGTTCTCATC
-
  >scaffold00003_27_28_474
+
  >scaffold00003_27_28_474_R
  GTACACCTCGTGCTCGGGACACAAATGGTC
  GTACACCTCGTGCTCGGGACACAAATGGTC
-
  >scaffold00003_28_29_210
+
 
 +
  >scaffold00003_28_29_210_F
  GTACACGTGCCGTTCCCGTTTCTGGTGAAC
  GTACACGTGCCGTTCCCGTTTCTGGTGAAC
-
  >scaffold00003_28_29_581
+
  >scaffold00003_28_29_173_R
-
  GAAGCTGTACCAGCTCCAGGACAACGACTG
+
  ACGGCGTCACGAACAACACCTACTG
-
>scaffold00003_29_30_703
+
 
-
TCAGCGATTCAGAGGTCCCGATCAGAGGTC
+
  >scaffold00003_30_31_256_F
-
>scaffold00003_29_30_1341
+
-
TACCTGCATACCGTACGTGGAGTCG
+
-
  >scaffold00003_30_31_256
+
  TTCCGGGGATGAGTAGCCGACCAATTGTCC
  TTCCGGGGATGAGTAGCCGACCAATTGTCC
-
  >scaffold00003_30_31_1239
+
  >scaffold00003_30_31_1239_R
  GATCACCGGTTGGTAGACCAGATCCAG
  GATCACCGGTTGGTAGACCAGATCCAG
-
  >scaffold00003_31_32_351
+
 
 +
  >scaffold00003_31_32_351_F
  CACAAATTGAACTTGAGCTGGGATGTCAGG
  CACAAATTGAACTTGAGCTGGGATGTCAGG
-
  >scaffold00003_31_32_565
+
  >scaffold00003_31_32_565_R
  CAGGATGACAGTGTGAGTCAGCCATGGAC
  CAGGATGACAGTGTGAGTCAGCCATGGAC
-
>scaffold00003_32_33_376
+
 
-
AGATCGACGAGCTCAACGTCTATCCG
+
  >scaffold00003_35_36_707_F
-
>scaffold00003_32_33_1069
+
-
GTCATTCGACACCCACCAGCAACGGATAG
+
-
>scaffold00003_33_34_1930
+
-
AAGCTCGACGCGATGCAGACCAACCTGAAC
+
-
>scaffold00003_33_34_485
+
-
CTCCAACTGCTCGGAGAGGAACTTGTAAC
+
-
>scaffold00003_34_35_491
+
-
GAACGAGACCAGGAGACATAGTGGCGAGTC
+
-
>scaffold00003_34_35_222
+
-
ATCTCCGCAGTCATATCGGTACGGG
+
-
  >scaffold00003_35_36_707
+
  AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
  AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
-
  >scaffold00003_35_36_202
+
  >scaffold00003_35_36_243_R
-
  TTGATCTTGTCGGCGAAGTCCGACAGCGAG
+
  CAGGTTGAACATCTCCTGGACCAGCGAAC
-
>scaffold00003_36_37_355
+
 
-
CTGCTCTTCCTCGGCATGCTCATGTTC
+
  >scaffold00003_42_43_317_F
-
>scaffold00003_36_37_430
+
-
ATAGCCCGCCCACTGCATGACAAAC
+
-
>scaffold00003_37_38_542
+
-
CGACTGGGAGTACTACATCGGTAACACC
+
-
>scaffold00003_37_38_565
+
-
GCACTGCGTGAACTCCCTTTCCATC
+
-
>scaffold00003_38_39_791
+
-
AACTGTCGACGAAGAACCGCGTCATGAGAG
+
-
>scaffold00003_38_39_613
+
-
GTACGAGCAGTTCACCAGTTGCGTG
+
-
>scaffold00003_39_40_351
+
-
CACTTGTCTTCTCCTCCGGATACCTGG
+
-
>scaffold00003_39_40_333
+
-
CTGATCCGTCATGGCGCACATTCCACGATG
+
-
>scaffold00003_40_41_413
+
-
GTAGGTCTGTGCCTGGTGGTTATGCCGATG
+
-
>scaffold00003_40_41_370
+
-
AAGTAGTGCGGCTCCTGCCATCTTCGTTTG
+
-
>scaffold00003_41_42_440
+
-
CTTCAGGTGATCGGCTCGATGGTAGGTCTG
+
-
>scaffold00003_41_42_495
+
-
CGTTCTTCACCCGGCTTCACATACTCG
+
-
  >scaffold00003_42_43_317
+
  GTTCCTGTCCTCGTGCAGCACAATTCGATC
  GTTCCTGTCCTCGTGCAGCACAATTCGATC
-
  >scaffold00003_42_43_284
+
  >scaffold00003_42_43_298_R
-
  ATCATCGTGGTCTTGTACGGCTCGCCCTTGAC
+
  ACCGGGGATCATCGTGGTCTTGTAC
-
  >scaffold00003_43_44_737
+
 
 +
  >scaffold00003_43_44_737_F
  ATCGTTCGCAGCGGCATCGATTCAC
  ATCGTTCGCAGCGGCATCGATTCAC
-
  >scaffold00003_43_44_992
+
  >scaffold00003_43_44_992_R
  TGAAGCTGACCAAGCTGGTGGTCTC
  TGAAGCTGACCAAGCTGGTGGTCTC
-
>scaffold00003_44_45_77029
+
 
-
Can't find proper primer
+
  >scaffold00003_45_46_422_F
-
>scaffold00003_44_45_224
+
-
AAGATCACCACGGCGTCGACACAGCTCAAC
+
-
  >scaffold00003_45_46_422
+
  GTGATGCCCTCGGATCCCAGTTCTTCGTAC
  GTGATGCCCTCGGATCCCAGTTCTTCGTAC
-
  >scaffold00003_45_46_399
+
  >scaffold00003_45_46_399_R
  AACTCGGTCAAGCTCGAGTTCCTGGTG
  AACTCGGTCAAGCTCGAGTTCCTGGTG
-
>scaffold00003_46_47_258
+
 
-
GACCTGCTGAGTGGATCATCAACCTATCG
+
  >scaffold00003_47_48_427_F
-
>scaffold00003_46_47_510
+
-
GTTGTAACCCATGGCTGACGAAGTAGGG
+
-
  >scaffold00003_47_48_427
+
  CATCACGGCGAACAACAACCAGACCTAC
  CATCACGGCGAACAACAACCAGACCTAC
-
  >scaffold00003_47_48_271
+
  >scaffold00003_47_48_271_R
  GAACAACGGCATGAGGTCCTTGAACATGAC
  GAACAACGGCATGAGGTCCTTGAACATGAC
-
  >scaffold00003_48_49_532
+
 
 +
  >scaffold00003_48_49_532_F
  GAAGGGTCAACAGTGAGATCGCCTGGTTC
  GAAGGGTCAACAGTGAGATCGCCTGGTTC
-
  >scaffold00003_48_49_741
+
  >scaffold00003_48_49_741_R
  TGTAGCTGCCCTTCTCCTCCTGTTC
  TGTAGCTGCCCTTCTCCTCCTGTTC
-
  >scaffold00003_49_50_208
+
 
 +
  >scaffold00003_49_50_208_F
  GTGGTGTGCATCGTGCAGATCCCGTTCTAC
  GTGGTGTGCATCGTGCAGATCCCGTTCTAC
-
  >scaffold00003_49_50_1069
+
  >scaffold00003_49_50_975_R
-
  CGCTCATCAGCAACATGCTCTGCAC
+
  AACCCGGAGCCGAAGAGTTCGAGAAAC
-
  >scaffold00003_50_51_1445
+
 
 +
  >scaffold00003_50_51_1445_F
  TGGCGTACCTGGAGAAGGTGGTTTC
  TGGCGTACCTGGAGAAGGTGGTTTC
-
  >scaffold00003_50_51_439
+
  >scaffold00003_50_51_439_R
  CAATGGCACCCTCACATATGGCAGATAATTG
  CAATGGCACCCTCACATATGGCAGATAATTG
-
>scaffold00004_1_2_381
+
 
-
GAACACGTCACAACCGTGCTTCCAG
+
  >scaffold00004_8_9_447_F
-
>scaffold00004_1_2_882
+
-
ATCAAGGTACTGCGGCTCAGTGAGAGCCTG
+
-
>scaffold00004_2_3_250
+
-
GTTGAACCGTCTGAACGACCGAGGAAG
+
-
>scaffold00004_2_3_411
+
-
TGTTGTCGGGGTAGGTGGGTAAACG
+
-
>scaffold00004_3_4_333
+
-
TGACTTCTCCGACCAGATCGCACACCTGTC
+
-
>scaffold00004_3_4_675
+
-
GAGCTTCTCCTTGTGCTGGGTCAAC
+
-
>scaffold00004_4_5_297
+
-
GGGAGGTTGTAGATCACGAACTGGATGTAG
+
-
>scaffold00004_4_5_180
+
-
CAGAACGAGTCCTACAACGGGAACCTTTC
+
-
>scaffold00004_5_6_411
+
-
GAGATCATGTCGCTGGGCACCAATC
+
-
>scaffold00004_5_6_891
+
-
GACATCGTGACCACCGTACTGGTCAAC
+
-
>scaffold00004_6_7_257
+
-
CGCGTTGAAAAACCGGGCGATATCC
+
-
>scaffold00004_6_7_545
+
-
GTCTTCGTGGTCAACAAGGCCGATC
+
-
>scaffold00004_7_8_584
+
-
TGATCAACGAGGCGTGCAACGGTAG
+
-
>scaffold00004_7_8_419
+
-
ATGATCGACACCGTTACGGCGAGATGG
+
-
  >scaffold00004_8_9_447
+
  GAACCTGCTGTTCATGGTCGGTCTG
  GAACCTGCTGTTCATGGTCGGTCTG
-
  >scaffold00004_8_9_234
+
  >scaffold00004_8_9_234_R
  ACAGTGGTCTCCGTCTACAGTCCAC
  ACAGTGGTCTCCGTCTACAGTCCAC
-
>scaffold00004_9_10_1726
+
 
-
TAGAGGGTCAACGGTTCGTCACCAG
+
  >scaffold00004_12_13_750_F
-
>scaffold00004_9_10_313
+
-
GTCAAGGATGACTGCGTGACTGGCTAC
+
-
>scaffold00004_10_11_783
+
-
AGAGCAGTTCGAGGATGTTGGGGTC
+
-
>scaffold00004_10_11_256
+
-
GCCGACTTCTACGTACTGCTGTCCATG
+
-
>scaffold00004_11_12_1607
+
-
TACCGACAAGTGAGAGGACGACGTG
+
-
>scaffold00004_11_12_704
+
-
GACCTGATCGCGGTGATTGGTTGAC
+
-
  >scaffold00004_12_13_750
+
  TCGATGGACATGCTCTCCTCCGAGATCGTC
  TCGATGGACATGCTCTCCTCCGAGATCGTC
-
  >scaffold00004_12_13_902
+
  >scaffold00004_12_13_895_R
-
  TGATCAATATGCGAGCGTCCGAAAAAGATC
+
  TATGCGAGCGTCCGAAAAAGATCGACCAGC
-
  >scaffold00004_13_14_550
+
 
 +
  >scaffold00004_13_14_550_F
  GAGCACATCAAGATCACTGACAATGGGATC
  GAGCACATCAAGATCACTGACAATGGGATC
-
  >scaffold00004_13_14_243
+
  >scaffold00004_13_14_243_R
  AACACTCCGAAATCCGCTGGCTCTC
  AACACTCCGAAATCCGCTGGCTCTC
-
  >scaffold00004_14_15_556
+
 
 +
  >scaffold00004_14_15_556_F
  CTGGTGGGACTCGTTGAGCATCCAATG
  CTGGTGGGACTCGTTGAGCATCCAATG
-
  >scaffold00004_14_15_220
+
  >scaffold00004_14_15_220_R
  CCGAGGGAAAGAACCGGACATTGTG
  CCGAGGGAAAGAACCGGACATTGTG
-
  >scaffold00004_15_16_1515
+
 
 +
  >scaffold00004_15_16_1515_F
  CTTCACCGGCCTGTTCTTCGTCATCAC
  CTTCACCGGCCTGTTCTTCGTCATCAC
-
  >scaffold00004_15_16_379
+
  >scaffold00004_15_16_167_R
-
  GATCGACAACCAGCCGAAGACGATG
+
  CAGTACCTGTGGCGTCACACGCGATAAGAG
-
  >scaffold00004_16_17_236
+
 
 +
  >scaffold00004_16_17_236_F
  GGTTCGACACCAGGATTTCGAGGTCAC
  GGTTCGACACCAGGATTTCGAGGTCAC
-
  >scaffold00004_16_17_467
+
  >scaffold00004_16_17_467_R
  GATCTGGTTCCACATCTGTCCCTGGTG
  GATCTGGTTCCACATCTGTCCCTGGTG
-
>scaffold00004_17_18_352
+
 
-
TGGAGGCATGACTTCCACACCTCAC
+
  >scaffold00004_18_19_226_F
-
>scaffold00004_17_18_720
+
-
CCAACATCAAGAGCCACCACAACGTC
+
-
  >scaffold00004_18_19_226
+
  GTGTGATCGACACGGTTACGTCGAAG
  GTGTGATCGACACGGTTACGTCGAAG
-
  >scaffold00004_18_19_545
+
  >scaffold00004_18_19_492_R
-
  CTGGTTCCGTCGACGAGGTGATAAGAAG
+
  TTAACAGGGGGCCCTTCCTTCAACACGGTC
-
>scaffold00004_19_20_273
+
 
-
GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC
+
  >scaffold00004_20_21_2075_F
-
>scaffold00004_19_20_878
+
  AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
-
GACGAGCAACTGGACTCGCTGAACAAG
+
  >scaffold00004_20_21_521_R
-
  >scaffold00004_20_21_1405
+
-
  CAGTTGGCCCTGCTTATCCTGCCGATGATG
+
-
  >scaffold00004_20_21_521
+
  TGAAGATCACCCAGGATCTCAAGGAGTACG
  TGAAGATCACCCAGGATCTCAAGGAGTACG
-
  >scaffold00004_21_22_386
+
 
 +
  >scaffold00004_21_22_386_F
  GAAGACTTCGCCACCGAATACCTCAGC
  GAAGACTTCGCCACCGAATACCTCAGC
-
  >scaffold00004_21_22_182
+
  >scaffold00004_21_22_182_R
  TTCGACGTACACACCACCCTCGCCGTAATC
  TTCGACGTACACACCACCCTCGCCGTAATC
-
>scaffold00004_22_23_594
+
 
-
AATTCAGCGGACAGCGGTACGAGGGTGTAG
+
  >scaffold00004_23_24_720_F
-
>scaffold00004_22_23_1225
+
  TCCACCGGAACATGGGTGATACTCGAC
-
AACAGGACCTGCTCGACAACAAGATCAAG
+
  >scaffold00004_23_24_552_R
-
  >scaffold00004_23_24_712
+
-
  AACATGGGTGATACTCGACGGGTGGCAGAC
+
-
  >scaffold00004_23_24_552
+
  GTTGCCTTTGCACAGGTAGATCCCG
  GTTGCCTTTGCACAGGTAGATCCCG
-
  >scaffold00004_24_25_667
+
 
 +
  >scaffold00004_24_25_667_F
  CAAGCCATGTCGAAGCAGCACTACTC
  CAAGCCATGTCGAAGCAGCACTACTC
-
  >scaffold00004_24_25_322
+
  >scaffold00004_24_25_322_R
  AAGCACCTGCTGTTCACAAGCGAACTGATC
  AAGCACCTGCTGTTCACAAGCGAACTGATC
-
  >scaffold00004_25_26_542
+
 
 +
  >scaffold00004_25_26_542_F
  GCAGATCGACGAGTACCTGGAGTTCTAC
  GCAGATCGACGAGTACCTGGAGTTCTAC
-
  >scaffold00004_25_26_235
+
  >scaffold00004_25_26_247_R
-
  ATGAAGATCAGGAAGATCGGCGCGAACAGC
+
  AGGAGTCCGACCATGAAGATCAGGAAGATC
-
  >scaffold00004_26_27_255
+
 
 +
>scaffold00004_29_30_278_F
 +
CATTTGTGACACCTGTCCAGCAGTACG
 +
>scaffold00004_29_30_264_R
 +
TCTGGTGAGCCGACAGGTATTCCTC
 +
 
 +
>scf1_10_11_191_R
 +
ATTCCTCGCGCAGTTGACCCAGTTC
 +
>scf1_10_11_533_F
 +
TTCGCCACCACCGGTCTAGGATGAGAGATC
 +
 +
>scf1_13_14_1221_R
 +
AGTCGTCGAAGCGCAGAAGGTACATGTAGG
 +
>scf1_13_14_961_F
 +
GTCATCGGACAGCCGATTCTTCGACGACTG
 +
 
 +
>scf1_14_15_1407_F
 +
CTTGTTTCGATAGCATTACCGCCCAGTAAC
 +
>scf1_14_15_533_R
 +
GTACTCGTCGAAGGTGGACACGTTCACTC
 +
 
 +
>scf1_16_17_1785_F
 +
CTCGAAGACGTCAACAACCACACCAATCTG
 +
>scf1_16_17_478_R
 +
CTCGTAACTCTCCATCGGTTCTCCAGTAAC
 +
 
 +
>scf1_22_23_238_F
 +
GTTTCACTCATCGGTGTACCCTCCCGATC
 +
>scf1_22_23_244_R
 +
GAAGTTCGTCATCGAGAACGTCTCCGTGC
 +
 
 +
>scf1_24_25_444_F
 +
ATTCGACGGGCAGGACGTCAACAAG
 +
>scf1_24_25_458_R
 +
TAGGTGTGCTCCAGGTAGGTGAGGAAC
 +
 
 +
>scf1_26_27_369_F
 +
ATGAGCGAGCAGGTCTTCCTCCTAC
 +
>scf1_26_27_706_R
 +
GAACACGCCGTAGTTGTTCAGCGTGAAG
 +
 
 +
>scf1_29_30_1643_F
 +
CCGATCCAGGCGAAGATCAGATTGGTC
 +
>scf1_29_30_767_R
 +
ATCGACATAGCGCTCCAACAGATCGGTGAC
 +
 
 +
>scf1_32_33_169_R
 +
TTGTCAATGTAGCGTTGACGGGTGCCTGAC
 +
>scf1_32_33_266_F
 +
AACATTGACAGGGTGGCACGCAGTTCG
 +
 
 +
>scf1_33_34_365_R
 +
GTTCCCTGCTGCTGAACTGGTTCTTCAC
 +
>scf1_33_34_795_F
 +
GTCGACGACGAGAATCCGGTTCATG
 +
 
 +
>scf1_37_38_498_F
 +
TTCGACGAACTGTGGCAACGCTTCTCC
 +
>scf1_37_38_562_R
 +
AGGACGTCATGCACGGTCAACACAC
 +
 
 +
>scf1_40_41_522_F
 +
GACTACAAGCCGGAGAACGTGCTCATCTC
 +
>scf1_40_41_660_R
 +
GTGCAGAAGGGATAGAGGGTGACGTAC
 +
 
 +
>scf1_43_44_440_F
 +
CTCGGAGGAAAACGGTGAACAGTCAGCAAC
 +
>scf1_43_44_563_R
 +
TGAACGCCTCCACCCAGCATTTGATCACAG
 +
 
 +
>scf1_44_45_485_R
 +
GTGTTGCGATCGGATTCGTAGCTGAC
 +
>scf1_44_45_93801_F
 +
ACACTGCTGCCGCCACGGTTGACTGT
 +
 
 +
>scf1_46_47_370_F
 +
GTAAACTCCTGATCTCAGCCGCTCTCGTG
 +
>scf1_46_47_845_R
 +
CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG
 +
 
 +
>scf1_53_54_414_F
 +
TTCTGGAAGACCATCTGCAGACCGATCTCG
 +
>scf1_53_54_823_R
 +
TACGCGAAGGGACGTTAGAGATGGCAC
 +
 
 +
>scf1_54_55_1250_F
 +
CTGGAACCGGATGATGTTGGAGTCCTC
 +
>scf1_54_55_175_R
 +
TACCGAGGAACTGATCGACCTCTCC
 +
 
 +
>scf1_56_57_184_R
 +
CAAACCGGTGGTCGGGCTGATCATGTATTC
 +
>scf1_56_57_412_F
 +
GTACTCACGATGGTCCTTCTCCTCGTTG
 +
 
 +
>scf1_59_60_1979_F
 +
GTGACCAGCCAGGTCTGTTCGAATCTCAC
 +
>scf1_59_60_625_R
 +
GAGATCATGTACGCCGACTTCAGCTTCGTG
 +
 
 +
>scf1_63_64_1286_R
 +
CGATAGGTGAGACTCGATGCATGGTTAGTG
 +
>scf1_63_64_711_F
 +
ACGTAGTTCCCGACCGTAGAGCAGTTC
 +
 
 +
>scf1_66_67_346_R
 +
ACTGTTCCTGCTGTACTCCTCGGTG
 +
>scf1_66_67_428_F
 +
CTTCGTCACTGGCTGTGTCAAGGTCAAC
 +
 
 +
>scf1_6_7_870_R
 +
CGATCTCCACGTTTGACAGGATGCTCTG
 +
>scf1_6_7_939_F
 +
ATCAGCGGTTACGTCTGGTGGTGAC
 +
 
 +
>scf1_7_8_545_R
 +
ATCAGTTTGATCGGCGAGACGTGCGAGACC
 +
>scf1_7_8_818_F
 +
GAACTGGAGTTGATGGAGCAGCGCGAAAC
 +
 
 +
>scf3_12_13_2070_F
 +
CTCCAGCAGGGTTTCATCGATTCGGTAG
 +
>scf3_12_13_324_R
 +
CTGGACCAGTTCACCCTCCAGATCATG
 +
 
 +
>scf3_18_19_471_R
 +
GTATACCGCAACATCGGCGAAATGAAGTC
 +
>scf3_18_19_746_F
 +
GTTGCTTCGAATCTGGTGACGGGAG
 +
 
 +
>scf3_20_21_200_R
 +
GAAGATCGGCAGCTCCATCGTCTCATC
 +
>scf3_20_21_294_F
 +
ATCAGCCCCGAGCAATTGACCGACCTGAAC
 +
 
 +
>scf3_21_22_1034_F
 +
GAAGTAACCGGTGGTGTTTTCGGCCAG
 +
>scf3_21_22_354_R
 +
ATCTCGCCGGTTACAAGCAGATCCTC
 +
 
 +
>scf3_22_23_1299_F
 +
TTCAGATGGTTAAGGACAGCTTCATGAGCG
 +
>scf3_22_23_368_R
 +
ACGTGATCGGTGGGAACTTCGACTC
 +
 
 +
>scf3_25_26_360_F
 +
GAAAGGCTGACCATCTACCCGGAGTAC
 +
>scf3_25_26_636_R
 +
TGATCCACGTGTCCGTACATCATCGTG
 +
 
 +
>scf3_33_34_1969_F
 +
GTCCTCAAGCATCGCAAGCGCAAGGAAAAG
 +
>scf3_33_34_485_R
 +
CTCCAACTGCTCGGAGAGGAACTTGTAAC
 +
 
 +
>scf3_34_35_222_R
 +
ATCTCCGCAGTCATATCGGTACGGG
 +
>scf3_34_35_491_F
 +
GAACGAGACCAGGAGACATAGTGGCGAGTC
 +
 
 +
>scf3_36_37_355_F
 +
CTGCTCTTCCTCGGCATGCTCATGTTC
 +
>scf3_36_37_430_R
 +
ATAGCCCGCCCACTGCATGACAAAC
 +
 
 +
>scf3_38_39_613_R
 +
GTACGAGCAGTTCACCAGTTGCGTG
 +
>scf3_38_39_791_F
 +
AACTGTCGACGAAGAACCGCGTCATGAGAG
 +
 
 +
>scf3_39_40_333_R
 +
CTGATCCGTCATGGCGCACATTCCACGATG
 +
>scf3_39_40_351_F
 +
CACTTGTCTTCTCCTCCGGATACCTGG
 +
 
 +
>scf3_44_45_1117_F
 +
CTCTGTGAGTACGTGGAACAGGCGATG
 +
>scf3_44_45_224_R
 +
AAGATCACCACGGCGTCGACACAGCTCAAC
 +
 
 +
>scf3_6_7_245_R
 +
GCATGCTGTTGTTGATGCTGTCCTTCAG
 +
>scf3_6_7_918_F
 +
ATGCTCAGTCCGGGAGTACAGCTCGGTAAC
 +
 
 +
>scf3_9_10_212_R
 +
TTTCCGTTGAGCCGCCACTCGTACTTGTAG
 +
>scf3_9_10_376_F
 +
CATGCACAAGCAGGACCTCATCGAG
 +
 
 +
>scf4_19_20_273_F
 +
GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC
 +
>scf4_19_20_878_R
 +
GACGAGCAACTGGACTCGCTGAACAAG
 +
 
 +
  >scf4_26_27_255_F
  GATCGTTCGACTACCCACAATCCGCAGATC
  GATCGTTCGACTACCCACAATCCGCAGATC
-
  >scaffold00004_26_27_671
+
  >scf4_26_27_671_R
  GGTAGAGCAGCCATTCCAGTTCCTGTG
  GGTAGAGCAGCCATTCCAGTTCCTGTG
-
>scaffold00004_27_28_577
+
 
-
CTGTTCACCGAGGAGGGCGAGTTCTTCTTC
+
  >scf4_27_28_485_R
-
  >scaffold00004_27_28_485
+
  GAAAACGGGGTGTTCCAGCTTCATCGCTTC
  GAAAACGGGGTGTTCCAGCTTCATCGCTTC
-
  >scaffold00004_28_29_1409
+
  >scf4_27_28_577_F
 +
CTGTTCACCGAGGAGGGCGAGTTCTTCTTC
 +
 
 +
>scf4_28_29_1409_F
  AACCGACACACCTGCAGTTGCCGTACCAAC
  AACCGACACACCTGCAGTTGCCGTACCAAC
-
  >scaffold00004_28_29_207
+
  >scf4_28_29_207_R
  TACTCCGGGATAAGCAAGGCTGAGCTG
  TACTCCGGGATAAGCAAGGCTGAGCTG
-
>scaffold00004_29_30_278
+
 
-
CATTTGTGACACCTGTCCAGCAGTACG
+
  >scf4_30_31_444_F
-
>scaffold00004_29_30_264
+
-
TCTGGTGAGCCGACAGGTATTCCTC
+
-
  >scaffold00004_30_31_444
+
  ACCTTCTTGCTCTTACGGCCCACCTTG
  ACCTTCTTGCTCTTACGGCCCACCTTG
-
  >scaffold00004_30_31_747
+
  >scf4_30_31_739_R
-
  GAGCCTCCGGAGTACGTCAAGTTCATTTTC
+
  GAGTACGTCAAGTTCATTTTCGCCACCAC
 +
 
 +
>scf3_1_2_276_F
 +
GACGTGTACATCTCGAACATCTCGCGG
 +
>scf3_1_2_387_R
 +
AAAACGGGCGTTCACCGCATGGTAC
 +
</pre>

Latest revision as of 16:12, 19 February 2011

Contents

Primer Naming Format

Gap primer format

ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R
 
                     CONTIG 1                                                     CONTIG 2
                                ------>
         5'                           3'  
 --------CTCAGGTCAGGCAGGCGTGATGATCTCTTC----------------NNNNNNNNN----------------------------------------
                                                                        GTTAGAGGAGAGTGCGGCTGGGCTCCAATCAT
                                                                        3'                             5'
                                                                        <-------

Positions:                      1105bp                                      529bp

List of primer

primers

 >scaffold00001_2_3_751_F
 CAGAACTGGAGAACGTCCATGCTCGAGAAC
 >scaffold00001_2_3_1360_R
 TCGATCACCAGCTCACGGAAGTCCTCGTTG

 >scaffold00001_3_4_586_F
 GTCAGTGCCGATCAGGATGTTGTCCAC
 >scaffold00001_3_4_183_R
 ATCAACTGCCAGAACTCGTCGGTCCTCATG

 >scaffold00001_4_5_568_F
 TACGTCGAGGAACTGGGCGACTTCAAGACC
 >scaffold00001_4_5_838_R
 CATGTCGTCGATCTGCACGTTGACCAG

 >scaffold00001_9_10_1135_F
 AGACCGGATTGAGTGCTTCCTCTGCTGGTG
 >scaffold00001_9_10_1408_R
 CGACCAATTTCCGACGAGGTGACATTC

 >scaffold00001_11_12_354_F
 AAGGTCAGGATGGCGGTGAACACCTTGGTC
 >scaffold00001_11_12_345_R
 ATGTTGTCGCTGACCTTCGCGATACGGGAG

 >scaffold00001_12_13_1313_F
 TTTCAGGCTTTGAGGATCGAGGTAAACAGG
 >scaffold00001_12_13_315_R
 TCGCATCTTCTCAGCCAGTTCCGAG

 >scaffold00001_15_16_126613_F
 GTTCCCGGTTGCGATCGGTGAGGAT
 >scaffold00001_15_16_569_R
 CTTCTACCACGACACACACACCGATCC

 >scaffold00001_17_18_225_F
 GATCGTGACGAACGTGAAGGTCCAC
 >scaffold00001_17_18_408_R
 CAGATCCGGTACAGCTCCTTGTATTCCAG

 >scaffold00001_18_19_320_F
 CAGATAGATGGTGAAGTCAGGACGACGG
 >scaffold00001_18_19_177_R
 ATGAACTTTCCACCGATGCCGGTCGTCTTC

 >scaffold00001_21_22_243_F
 AAGGACGGACGTGGACATCGATCAGCAATC
 >scaffold00001_21_22_1379_R
 AGGACTCCTGTACGTCGATCACGACGAGTG

 >scaffold00001_23_24_410_F
 ACTGACCACGGTGCAGCTCTACTTCAC
 >scaffold00001_23_24_1184_R
 AGCGTGTAGTCGGCGGTATACACCTTC

 >scaffold00001_25_26_203_F
 GTCGAGGGGATCAACACAAGTGGAATCAAG
 >scaffold00001_25_26_184_R
 GTACCGTCGACAAGCAACTCCTGATCGTC

 >scaffold00001_27_28_280_F
 TGACGTGAGGGGACTACGGATGTAC
 >scaffold00001_27_28_260_R
 ACTTCGACAGGTAGGCCTTGCAGAG

 >scaffold00001_31_32_566_F
 AAACGAGCCGTACAACGGGCAACTG
 >scaffold00001_31_32_169_R
 ATCTTCCTGACCTCGTCGGTGTGGTACTTC

 >scaffold00001_34_35_1071_F
 CAGTACGTCATCTTCGACCGGACCATTTG
 >scaffold00001_34_35_2096_R
 AGTCGTCGTCGTCGAACATCGTGAC

 >scaffold00001_35_36_685_F
 GTCGTTCAGGTTCAGGTAGAACTCGGTGAC
 >scaffold00001_35_36_0_R
 CCCGACCGAACGGCACGCACACAA

 >scaffold00001_36_37_1073_F
 GATCGGTTTGCCGCGGTCGTTGTATTCGAC
 >scaffold00001_36_37_740_R
 ATACGGCTTCGTCGAGAACCTGACC

 >scaffold00001_38_39_612_F
 AACATGTCGTTGAGCGCGGCCAGTTTG
 >scaffold00001_38_39_672_R
 CTCTTCGACGAGATCATCCAGGTGCTG

 >scaffold00001_41_42_205_F
 ACCGAAGCAGTCGAAGACGGTACAC
 >scaffold00001_41_42_339_R
 GTCTTCTTCTGGCCCCGCAACGAGATCATG

 >scaffold00001_42_43_389_F
 TAAAATCTGTGATCTGGGCTAACCTTGGGG
 >scaffold00001_42_43_223_R
 GTGATCGGTGTGTTCGTGGCTGACCAGTAC

 >scaffold00001_45_46_401_F
 GGATCAGCTCCGGATAGTTGAAGTCGTC
 >scaffold00001_45_46_423_R
 ACAACGAGGACTTCGACAACGGCTTCAAGG

 >scaffold00001_47_48_1970_F
 GACGACTACCTGCGTAAACCCTTCGACCTG
 >scaffold00001_47_48_1044_R
 CACCAATCTGCTTGTGGGTCTGGTGCTGAC

 >scaffold00001_48_49_1639_F
 GATGTTCATCGACCTGGAGAACGTGC
 >scaffold00001_48_49_244_R
 CATCTCTCCCGTCAGCTCATCGTTCGAAG

 >scaffold00001_49_50_1297_F
 CAGGGTTTCCTGAGCCACCTTGAAC
 >scaffold00001_49_50_822_R
 CTGTTCTCCCAGGACCCGATCTTCAACTC

 >scaffold00001_50_51_227_F
 CGTGCCATGTTCTGAACGAGAACGATC
 >scaffold00001_50_51_860_R
 GTGACCAGCGAGTGAATCTTGTCGCGTTG

 >scaffold00001_55_56_239_F
 CACCTGACCTCTGACAATGAACGCCGAAAG
 >scaffold00001_55_56_206_R
 GATTGGCTTCTATACAAACTAAGGTGGCGC

 >scaffold00001_57_58_1204_F
 TCATGCTGTCCGTATCCATTTAGGACCTTC
 >scaffold00001_57_58_939_R
 TTCTACCGTGCCTACTTCGAGACCGAC

 >scaffold00001_61_62_285_F
 TCAGGTTGAGATCGACCATCGCGAG
 >scaffold00001_61_62_944_R
 TTGCACCATCAGCTCAGCATCGTCTTCGAC

 >scaffold00001_62_63_898_F
 CTGATGATCAAGGTGTTGCGGCTGTC
 >scaffold00001_62_63_367_R
 GTTGTAGCCGCCGAAGTCGTTCATGCTGAC

 >scaffold00001_67_68_5122_F
 GACCGTTGCGACCAGATATTCGGCGATT
 >scaffold00001_67_68_0_R
 AACCGCTGACCGCGTTCGTGCTGTTCT

 >scaffold00001_71_72_349_F
 AACCCACGCATCCCACTCAACGAGCTGAAC
 >scaffold00001_71_72_0_R
 TCGTTGTCCACCACGCCGACGAACA

 >scaffold00001_73_74_2968_F
 TACGCGGCAGTCGGACGGGTGTGTT
 >scaffold00001_73_74_0_R
 GCTCACCGTGCCGGTGAGGACCAA

 >scaffold00001_75_76_788_F
 GTCGACGTTTCATCACGTCACTGGGTG
 >scaffold00001_75_76_0_R
 GTGCGCCAAGACCTGCGCGAACAGT

 >scaffold00001_76_77_1854_F
 GTGATCAAGGTGATCGAAGCCCTGC
 >scaffold00001_76_77_535_R
 ATCTACAACCACCTGGGCGGCAAGGAGAAC

 >scaffold00001_80_81_426_F
 CACTGCCCAAACCGATCTATCCGACGTTC
 >scaffold00001_80_81_202_R
 GATCCAAGCTGTCTTAACGAGGAGTTGCC

 >scaffold00001_87_88_204_F
 GAAGTGATCACGCGATCCACGGGATTCCAC
 >scaffold00001_87_88_267_R
 AGCTTCCAGACCCTGGTCAACAGTC

 >scaffold00001_88_89_1973_F
 AGTAGCCGATGACCCCGTTGAGTTG
 >scaffold00001_88_89_463_R
 CAGGTGTTCAACCTCGCCCAGACCTTCTAC

 >scaffold00003_3_4_218_F
 ACAAGCTCATTGCCTACTAGATCGACTTCG
 >scaffold00003_3_4_1054_R
 GCATCGTCCAACGGTAGCCTTCTTTC

 >scaffold00003_7_8_365_F
 ATCCGACGAGTACGACATCCACCTG
 >scaffold00003_7_8_601_R
 CAGACCAACTACGCCACAAGCAAGG

 >scaffold00003_8_9_472_F
 CTGTTCCAGACCTTCCTCACCGTGCTTGAC
 >scaffold00003_8_9_218_R
 CTTATGCCCTGACAGAAGGTGCAAGATCAG

 >scaffold00003_10_11_1436_F
 TGGTCCTGCCGTACTTCCTGATCACCACTG
 >scaffold00003_10_11_232_R
 GATGGGACAGACTTCGTACACCGGTTG

 >scaffold00003_11_12_266_F
 CTGTATTGATGATCCACTCGCTGCCGCTC
 >scaffold00003_11_12_1184_R
 TACAGTGATCGGCCGTTGTTGGAGTGTGTG

 >scaffold00003_17_18_314_F
 TCTGAACGAGACGGAATGGGAGATCCTCAC
 >scaffold00003_17_18_222_R
 GACATCACCATCCAGGCAGAGAACATGCTG

 >scaffold00003_19_20_655_F
 GAAGGAACGGCAACTTTCGGTCACGGTAC
 >scaffold00003_19_20_485_R
 CAAGGAGACCGTCTACAAGATCTGGCATC

 >scaffold00003_23_24_235_F
 CCCAGGGAATATAACGATGGTTCAGGTCAG
 >scaffold00003_23_24_1163_R
 GAACTGCTGGTTCATCGAATGGTCGTTGAC

 >scaffold00003_24_25_268_F
 CTCGAAGTCCCGGTCAAGAAGATCCTCAC
 >scaffold00003_24_25_666_R
 TATCGTTCGCGTCGATGGCCGACAACATTG

 >scaffold00003_26_27_1270_F
 GAAATCAAGATCCTGAACCAGTCCCGGAAG
 >scaffold00003_26_27_244_R
 CCCGATTCCTTGTCCTGGAACAGATAGAAG

 >scaffold00003_27_28_833_F
 GTACTCGCCGACTTTCCCGTTCTCATC
 >scaffold00003_27_28_474_R
 GTACACCTCGTGCTCGGGACACAAATGGTC

 >scaffold00003_28_29_210_F
 GTACACGTGCCGTTCCCGTTTCTGGTGAAC
 >scaffold00003_28_29_173_R
 ACGGCGTCACGAACAACACCTACTG

 >scaffold00003_30_31_256_F
 TTCCGGGGATGAGTAGCCGACCAATTGTCC
 >scaffold00003_30_31_1239_R
 GATCACCGGTTGGTAGACCAGATCCAG

 >scaffold00003_31_32_351_F
 CACAAATTGAACTTGAGCTGGGATGTCAGG
 >scaffold00003_31_32_565_R
 CAGGATGACAGTGTGAGTCAGCCATGGAC

 >scaffold00003_35_36_707_F
 AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
 >scaffold00003_35_36_243_R
 CAGGTTGAACATCTCCTGGACCAGCGAAC

 >scaffold00003_42_43_317_F
 GTTCCTGTCCTCGTGCAGCACAATTCGATC
 >scaffold00003_42_43_298_R
 ACCGGGGATCATCGTGGTCTTGTAC

 >scaffold00003_43_44_737_F
 ATCGTTCGCAGCGGCATCGATTCAC
 >scaffold00003_43_44_992_R
 TGAAGCTGACCAAGCTGGTGGTCTC

 >scaffold00003_45_46_422_F
 GTGATGCCCTCGGATCCCAGTTCTTCGTAC
 >scaffold00003_45_46_399_R
 AACTCGGTCAAGCTCGAGTTCCTGGTG

 >scaffold00003_47_48_427_F
 CATCACGGCGAACAACAACCAGACCTAC
 >scaffold00003_47_48_271_R
 GAACAACGGCATGAGGTCCTTGAACATGAC

 >scaffold00003_48_49_532_F
 GAAGGGTCAACAGTGAGATCGCCTGGTTC
 >scaffold00003_48_49_741_R
 TGTAGCTGCCCTTCTCCTCCTGTTC

 >scaffold00003_49_50_208_F
 GTGGTGTGCATCGTGCAGATCCCGTTCTAC
 >scaffold00003_49_50_975_R
 AACCCGGAGCCGAAGAGTTCGAGAAAC

 >scaffold00003_50_51_1445_F
 TGGCGTACCTGGAGAAGGTGGTTTC
 >scaffold00003_50_51_439_R
 CAATGGCACCCTCACATATGGCAGATAATTG

 >scaffold00004_8_9_447_F
 GAACCTGCTGTTCATGGTCGGTCTG
 >scaffold00004_8_9_234_R
 ACAGTGGTCTCCGTCTACAGTCCAC

 >scaffold00004_12_13_750_F
 TCGATGGACATGCTCTCCTCCGAGATCGTC
 >scaffold00004_12_13_895_R
 TATGCGAGCGTCCGAAAAAGATCGACCAGC

 >scaffold00004_13_14_550_F
 GAGCACATCAAGATCACTGACAATGGGATC
 >scaffold00004_13_14_243_R
 AACACTCCGAAATCCGCTGGCTCTC

 >scaffold00004_14_15_556_F
 CTGGTGGGACTCGTTGAGCATCCAATG
 >scaffold00004_14_15_220_R
 CCGAGGGAAAGAACCGGACATTGTG

 >scaffold00004_15_16_1515_F
 CTTCACCGGCCTGTTCTTCGTCATCAC
 >scaffold00004_15_16_167_R
 CAGTACCTGTGGCGTCACACGCGATAAGAG

 >scaffold00004_16_17_236_F
 GGTTCGACACCAGGATTTCGAGGTCAC
 >scaffold00004_16_17_467_R
 GATCTGGTTCCACATCTGTCCCTGGTG

 >scaffold00004_18_19_226_F
 GTGTGATCGACACGGTTACGTCGAAG
 >scaffold00004_18_19_492_R
 TTAACAGGGGGCCCTTCCTTCAACACGGTC

 >scaffold00004_20_21_2075_F
 AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
 >scaffold00004_20_21_521_R
 TGAAGATCACCCAGGATCTCAAGGAGTACG

 >scaffold00004_21_22_386_F
 GAAGACTTCGCCACCGAATACCTCAGC
 >scaffold00004_21_22_182_R
 TTCGACGTACACACCACCCTCGCCGTAATC

 >scaffold00004_23_24_720_F
 TCCACCGGAACATGGGTGATACTCGAC
 >scaffold00004_23_24_552_R
 GTTGCCTTTGCACAGGTAGATCCCG

 >scaffold00004_24_25_667_F
 CAAGCCATGTCGAAGCAGCACTACTC
 >scaffold00004_24_25_322_R
 AAGCACCTGCTGTTCACAAGCGAACTGATC

 >scaffold00004_25_26_542_F
 GCAGATCGACGAGTACCTGGAGTTCTAC
 >scaffold00004_25_26_247_R
 AGGAGTCCGACCATGAAGATCAGGAAGATC

 >scaffold00004_29_30_278_F
 CATTTGTGACACCTGTCCAGCAGTACG
 >scaffold00004_29_30_264_R
 TCTGGTGAGCCGACAGGTATTCCTC

 >scf1_10_11_191_R
 ATTCCTCGCGCAGTTGACCCAGTTC
 >scf1_10_11_533_F
 TTCGCCACCACCGGTCTAGGATGAGAGATC
 
 >scf1_13_14_1221_R
 AGTCGTCGAAGCGCAGAAGGTACATGTAGG
 >scf1_13_14_961_F
 GTCATCGGACAGCCGATTCTTCGACGACTG

 >scf1_14_15_1407_F
 CTTGTTTCGATAGCATTACCGCCCAGTAAC
 >scf1_14_15_533_R
 GTACTCGTCGAAGGTGGACACGTTCACTC

 >scf1_16_17_1785_F
 CTCGAAGACGTCAACAACCACACCAATCTG
 >scf1_16_17_478_R
 CTCGTAACTCTCCATCGGTTCTCCAGTAAC

 >scf1_22_23_238_F
 GTTTCACTCATCGGTGTACCCTCCCGATC
 >scf1_22_23_244_R
 GAAGTTCGTCATCGAGAACGTCTCCGTGC

 >scf1_24_25_444_F
 ATTCGACGGGCAGGACGTCAACAAG
 >scf1_24_25_458_R
 TAGGTGTGCTCCAGGTAGGTGAGGAAC

 >scf1_26_27_369_F
 ATGAGCGAGCAGGTCTTCCTCCTAC
 >scf1_26_27_706_R
 GAACACGCCGTAGTTGTTCAGCGTGAAG

 >scf1_29_30_1643_F
 CCGATCCAGGCGAAGATCAGATTGGTC
 >scf1_29_30_767_R
 ATCGACATAGCGCTCCAACAGATCGGTGAC

 >scf1_32_33_169_R
 TTGTCAATGTAGCGTTGACGGGTGCCTGAC
 >scf1_32_33_266_F
 AACATTGACAGGGTGGCACGCAGTTCG

 >scf1_33_34_365_R
 GTTCCCTGCTGCTGAACTGGTTCTTCAC
 >scf1_33_34_795_F
 GTCGACGACGAGAATCCGGTTCATG

 >scf1_37_38_498_F
 TTCGACGAACTGTGGCAACGCTTCTCC
 >scf1_37_38_562_R
 AGGACGTCATGCACGGTCAACACAC

 >scf1_40_41_522_F
 GACTACAAGCCGGAGAACGTGCTCATCTC
 >scf1_40_41_660_R
 GTGCAGAAGGGATAGAGGGTGACGTAC

 >scf1_43_44_440_F
 CTCGGAGGAAAACGGTGAACAGTCAGCAAC
 >scf1_43_44_563_R
 TGAACGCCTCCACCCAGCATTTGATCACAG

 >scf1_44_45_485_R
 GTGTTGCGATCGGATTCGTAGCTGAC
 >scf1_44_45_93801_F
 ACACTGCTGCCGCCACGGTTGACTGT

 >scf1_46_47_370_F
 GTAAACTCCTGATCTCAGCCGCTCTCGTG
 >scf1_46_47_845_R
 CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG

 >scf1_53_54_414_F
 TTCTGGAAGACCATCTGCAGACCGATCTCG
 >scf1_53_54_823_R
 TACGCGAAGGGACGTTAGAGATGGCAC

 >scf1_54_55_1250_F
 CTGGAACCGGATGATGTTGGAGTCCTC
 >scf1_54_55_175_R
 TACCGAGGAACTGATCGACCTCTCC

 >scf1_56_57_184_R
 CAAACCGGTGGTCGGGCTGATCATGTATTC
 >scf1_56_57_412_F
 GTACTCACGATGGTCCTTCTCCTCGTTG

 >scf1_59_60_1979_F
 GTGACCAGCCAGGTCTGTTCGAATCTCAC
 >scf1_59_60_625_R
 GAGATCATGTACGCCGACTTCAGCTTCGTG

 >scf1_63_64_1286_R
 CGATAGGTGAGACTCGATGCATGGTTAGTG
 >scf1_63_64_711_F
 ACGTAGTTCCCGACCGTAGAGCAGTTC

 >scf1_66_67_346_R
 ACTGTTCCTGCTGTACTCCTCGGTG
 >scf1_66_67_428_F
 CTTCGTCACTGGCTGTGTCAAGGTCAAC

 >scf1_6_7_870_R
 CGATCTCCACGTTTGACAGGATGCTCTG
 >scf1_6_7_939_F
 ATCAGCGGTTACGTCTGGTGGTGAC

 >scf1_7_8_545_R
 ATCAGTTTGATCGGCGAGACGTGCGAGACC
 >scf1_7_8_818_F
 GAACTGGAGTTGATGGAGCAGCGCGAAAC

 >scf3_12_13_2070_F
 CTCCAGCAGGGTTTCATCGATTCGGTAG
 >scf3_12_13_324_R
 CTGGACCAGTTCACCCTCCAGATCATG

 >scf3_18_19_471_R
 GTATACCGCAACATCGGCGAAATGAAGTC
 >scf3_18_19_746_F
 GTTGCTTCGAATCTGGTGACGGGAG

 >scf3_20_21_200_R
 GAAGATCGGCAGCTCCATCGTCTCATC
 >scf3_20_21_294_F
 ATCAGCCCCGAGCAATTGACCGACCTGAAC

 >scf3_21_22_1034_F
 GAAGTAACCGGTGGTGTTTTCGGCCAG
 >scf3_21_22_354_R
 ATCTCGCCGGTTACAAGCAGATCCTC

 >scf3_22_23_1299_F
 TTCAGATGGTTAAGGACAGCTTCATGAGCG
 >scf3_22_23_368_R
 ACGTGATCGGTGGGAACTTCGACTC

 >scf3_25_26_360_F
 GAAAGGCTGACCATCTACCCGGAGTAC
 >scf3_25_26_636_R
 TGATCCACGTGTCCGTACATCATCGTG

 >scf3_33_34_1969_F
 GTCCTCAAGCATCGCAAGCGCAAGGAAAAG
 >scf3_33_34_485_R
 CTCCAACTGCTCGGAGAGGAACTTGTAAC

 >scf3_34_35_222_R
 ATCTCCGCAGTCATATCGGTACGGG
 >scf3_34_35_491_F
 GAACGAGACCAGGAGACATAGTGGCGAGTC

 >scf3_36_37_355_F
 CTGCTCTTCCTCGGCATGCTCATGTTC
 >scf3_36_37_430_R
 ATAGCCCGCCCACTGCATGACAAAC

 >scf3_38_39_613_R
 GTACGAGCAGTTCACCAGTTGCGTG
 >scf3_38_39_791_F
 AACTGTCGACGAAGAACCGCGTCATGAGAG

 >scf3_39_40_333_R
 CTGATCCGTCATGGCGCACATTCCACGATG
 >scf3_39_40_351_F
 CACTTGTCTTCTCCTCCGGATACCTGG

 >scf3_44_45_1117_F
 CTCTGTGAGTACGTGGAACAGGCGATG
 >scf3_44_45_224_R
 AAGATCACCACGGCGTCGACACAGCTCAAC

 >scf3_6_7_245_R
 GCATGCTGTTGTTGATGCTGTCCTTCAG
 >scf3_6_7_918_F
 ATGCTCAGTCCGGGAGTACAGCTCGGTAAC

 >scf3_9_10_212_R
 TTTCCGTTGAGCCGCCACTCGTACTTGTAG
 >scf3_9_10_376_F
 CATGCACAAGCAGGACCTCATCGAG

 >scf4_19_20_273_F
 GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC
 >scf4_19_20_878_R
 GACGAGCAACTGGACTCGCTGAACAAG

 >scf4_26_27_255_F
 GATCGTTCGACTACCCACAATCCGCAGATC
 >scf4_26_27_671_R
 GGTAGAGCAGCCATTCCAGTTCCTGTG

 >scf4_27_28_485_R
 GAAAACGGGGTGTTCCAGCTTCATCGCTTC
 >scf4_27_28_577_F
 CTGTTCACCGAGGAGGGCGAGTTCTTCTTC

 >scf4_28_29_1409_F
 AACCGACACACCTGCAGTTGCCGTACCAAC
 >scf4_28_29_207_R
 TACTCCGGGATAAGCAAGGCTGAGCTG

 >scf4_30_31_444_F
 ACCTTCTTGCTCTTACGGCCCACCTTG
 >scf4_30_31_739_R
 GAGTACGTCAAGTTCATTTTCGCCACCAC

 >scf3_1_2_276_F
 GACGTGTACATCTCGAACATCTCGCGG
 >scf3_1_2_387_R
 AAAACGGGCGTTCACCGCATGGTAC
Retrieved from "http://compbio.korea.ac.kr/wiki/index.php/Primer_Verrucosispora_gap"
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