Primer Verrucosispora gap

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==Primer Naming Format==
==Primer Naming Format==
-
===Scf&SolCtg primer format===
+
<!-- ===Scf&SolCtg primer format=== -->
===Gap primer format===
===Gap primer format===
*>[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse]
*>[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse]
-
ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R
+
<pre>
 +
ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R
   
   
                     CONTIG 1                                                    CONTIG 2
                     CONTIG 1                                                    CONTIG 2
Line 13: Line 14:
                                                                         <-------
                                                                         <-------
-
                                            1105bp               529bp
+
Positions:                      1105bp                                     529bp
 +
</pre>
==List of primer==
==List of primer==
 +
<!--
===primers of scaffold & sole contigs===
===primers of scaffold & sole contigs===
====scf1====
====scf1====
Line 52: Line 55:
  TGGCGATTGGGACGAAGTCGTAACAAGGTA
  TGGCGATTGGGACGAAGTCGTAACAAGGTA
 +
-->
*By Using Primer3
*By Using Primer3
*"Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer).
*"Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer).
-
===primer intergenic===
+
 
 +
===primers===
 +
*115 gaps (230 primers)
 +
 
 +
<pre>
  >scaffold00001_2_3_751_F
  >scaffold00001_2_3_751_F
  CAGAACTGGAGAACGTCCATGCTCGAGAAC
  CAGAACTGGAGAACGTCCATGCTCGAGAAC
  >scaffold00001_2_3_1360_R
  >scaffold00001_2_3_1360_R
  TCGATCACCAGCTCACGGAAGTCCTCGTTG
  TCGATCACCAGCTCACGGAAGTCCTCGTTG
 +
  >scaffold00001_3_4_586_F
  >scaffold00001_3_4_586_F
  GTCAGTGCCGATCAGGATGTTGTCCAC
  GTCAGTGCCGATCAGGATGTTGTCCAC
  >scaffold00001_3_4_183_R
  >scaffold00001_3_4_183_R
  ATCAACTGCCAGAACTCGTCGGTCCTCATG
  ATCAACTGCCAGAACTCGTCGGTCCTCATG
 +
  >scaffold00001_4_5_568_F
  >scaffold00001_4_5_568_F
  TACGTCGAGGAACTGGGCGACTTCAAGACC
  TACGTCGAGGAACTGGGCGACTTCAAGACC
  >scaffold00001_4_5_838_R
  >scaffold00001_4_5_838_R
  CATGTCGTCGATCTGCACGTTGACCAG
  CATGTCGTCGATCTGCACGTTGACCAG
 +
  >scaffold00001_9_10_1135_F
  >scaffold00001_9_10_1135_F
  AGACCGGATTGAGTGCTTCCTCTGCTGGTG
  AGACCGGATTGAGTGCTTCCTCTGCTGGTG
  >scaffold00001_9_10_1408_R
  >scaffold00001_9_10_1408_R
  CGACCAATTTCCGACGAGGTGACATTC
  CGACCAATTTCCGACGAGGTGACATTC
 +
  >scaffold00001_11_12_354_F
  >scaffold00001_11_12_354_F
  AAGGTCAGGATGGCGGTGAACACCTTGGTC
  AAGGTCAGGATGGCGGTGAACACCTTGGTC
  >scaffold00001_11_12_345_R
  >scaffold00001_11_12_345_R
  ATGTTGTCGCTGACCTTCGCGATACGGGAG
  ATGTTGTCGCTGACCTTCGCGATACGGGAG
 +
  >scaffold00001_12_13_1313_F
  >scaffold00001_12_13_1313_F
  TTTCAGGCTTTGAGGATCGAGGTAAACAGG
  TTTCAGGCTTTGAGGATCGAGGTAAACAGG
  >scaffold00001_12_13_315_R
  >scaffold00001_12_13_315_R
  TCGCATCTTCTCAGCCAGTTCCGAG
  TCGCATCTTCTCAGCCAGTTCCGAG
 +
  >scaffold00001_15_16_126613_F
  >scaffold00001_15_16_126613_F
-
  Can't find proper primer
+
  GTTCCCGGTTGCGATCGGTGAGGAT
  >scaffold00001_15_16_569_R
  >scaffold00001_15_16_569_R
  CTTCTACCACGACACACACACCGATCC
  CTTCTACCACGACACACACACCGATCC
 +
  >scaffold00001_17_18_225_F
  >scaffold00001_17_18_225_F
  GATCGTGACGAACGTGAAGGTCCAC
  GATCGTGACGAACGTGAAGGTCCAC
  >scaffold00001_17_18_408_R
  >scaffold00001_17_18_408_R
  CAGATCCGGTACAGCTCCTTGTATTCCAG
  CAGATCCGGTACAGCTCCTTGTATTCCAG
 +
  >scaffold00001_18_19_320_F
  >scaffold00001_18_19_320_F
  CAGATAGATGGTGAAGTCAGGACGACGG
  CAGATAGATGGTGAAGTCAGGACGACGG
  >scaffold00001_18_19_177_R
  >scaffold00001_18_19_177_R
  ATGAACTTTCCACCGATGCCGGTCGTCTTC
  ATGAACTTTCCACCGATGCCGGTCGTCTTC
 +
  >scaffold00001_21_22_243_F
  >scaffold00001_21_22_243_F
  AAGGACGGACGTGGACATCGATCAGCAATC
  AAGGACGGACGTGGACATCGATCAGCAATC
  >scaffold00001_21_22_1379_R
  >scaffold00001_21_22_1379_R
  AGGACTCCTGTACGTCGATCACGACGAGTG
  AGGACTCCTGTACGTCGATCACGACGAGTG
 +
  >scaffold00001_23_24_410_F
  >scaffold00001_23_24_410_F
  ACTGACCACGGTGCAGCTCTACTTCAC
  ACTGACCACGGTGCAGCTCTACTTCAC
  >scaffold00001_23_24_1184_R
  >scaffold00001_23_24_1184_R
  AGCGTGTAGTCGGCGGTATACACCTTC
  AGCGTGTAGTCGGCGGTATACACCTTC
 +
  >scaffold00001_25_26_203_F
  >scaffold00001_25_26_203_F
  GTCGAGGGGATCAACACAAGTGGAATCAAG
  GTCGAGGGGATCAACACAAGTGGAATCAAG
  >scaffold00001_25_26_184_R
  >scaffold00001_25_26_184_R
  GTACCGTCGACAAGCAACTCCTGATCGTC
  GTACCGTCGACAAGCAACTCCTGATCGTC
 +
  >scaffold00001_27_28_280_F
  >scaffold00001_27_28_280_F
  TGACGTGAGGGGACTACGGATGTAC
  TGACGTGAGGGGACTACGGATGTAC
  >scaffold00001_27_28_260_R
  >scaffold00001_27_28_260_R
  ACTTCGACAGGTAGGCCTTGCAGAG
  ACTTCGACAGGTAGGCCTTGCAGAG
 +
  >scaffold00001_31_32_566_F
  >scaffold00001_31_32_566_F
  AAACGAGCCGTACAACGGGCAACTG
  AAACGAGCCGTACAACGGGCAACTG
  >scaffold00001_31_32_169_R
  >scaffold00001_31_32_169_R
  ATCTTCCTGACCTCGTCGGTGTGGTACTTC
  ATCTTCCTGACCTCGTCGGTGTGGTACTTC
 +
  >scaffold00001_34_35_1071_F
  >scaffold00001_34_35_1071_F
  CAGTACGTCATCTTCGACCGGACCATTTG
  CAGTACGTCATCTTCGACCGGACCATTTG
  >scaffold00001_34_35_2096_R
  >scaffold00001_34_35_2096_R
  AGTCGTCGTCGTCGAACATCGTGAC
  AGTCGTCGTCGTCGAACATCGTGAC
 +
  >scaffold00001_35_36_685_F
  >scaffold00001_35_36_685_F
  GTCGTTCAGGTTCAGGTAGAACTCGGTGAC
  GTCGTTCAGGTTCAGGTAGAACTCGGTGAC
  >scaffold00001_35_36_0_R
  >scaffold00001_35_36_0_R
-
  Can't find proper primer
+
  CCCGACCGAACGGCACGCACACAA
 +
 
  >scaffold00001_36_37_1073_F
  >scaffold00001_36_37_1073_F
  GATCGGTTTGCCGCGGTCGTTGTATTCGAC
  GATCGGTTTGCCGCGGTCGTTGTATTCGAC
  >scaffold00001_36_37_740_R
  >scaffold00001_36_37_740_R
  ATACGGCTTCGTCGAGAACCTGACC
  ATACGGCTTCGTCGAGAACCTGACC
 +
  >scaffold00001_38_39_612_F
  >scaffold00001_38_39_612_F
  AACATGTCGTTGAGCGCGGCCAGTTTG
  AACATGTCGTTGAGCGCGGCCAGTTTG
  >scaffold00001_38_39_672_R
  >scaffold00001_38_39_672_R
  CTCTTCGACGAGATCATCCAGGTGCTG
  CTCTTCGACGAGATCATCCAGGTGCTG
 +
  >scaffold00001_41_42_205_F
  >scaffold00001_41_42_205_F
  ACCGAAGCAGTCGAAGACGGTACAC
  ACCGAAGCAGTCGAAGACGGTACAC
  >scaffold00001_41_42_339_R
  >scaffold00001_41_42_339_R
  GTCTTCTTCTGGCCCCGCAACGAGATCATG
  GTCTTCTTCTGGCCCCGCAACGAGATCATG
 +
  >scaffold00001_42_43_389_F
  >scaffold00001_42_43_389_F
  TAAAATCTGTGATCTGGGCTAACCTTGGGG
  TAAAATCTGTGATCTGGGCTAACCTTGGGG
  >scaffold00001_42_43_223_R
  >scaffold00001_42_43_223_R
  GTGATCGGTGTGTTCGTGGCTGACCAGTAC
  GTGATCGGTGTGTTCGTGGCTGACCAGTAC
 +
  >scaffold00001_45_46_401_F
  >scaffold00001_45_46_401_F
  GGATCAGCTCCGGATAGTTGAAGTCGTC
  GGATCAGCTCCGGATAGTTGAAGTCGTC
  >scaffold00001_45_46_423_R
  >scaffold00001_45_46_423_R
  ACAACGAGGACTTCGACAACGGCTTCAAGG
  ACAACGAGGACTTCGACAACGGCTTCAAGG
 +
  >scaffold00001_47_48_1970_F
  >scaffold00001_47_48_1970_F
  GACGACTACCTGCGTAAACCCTTCGACCTG
  GACGACTACCTGCGTAAACCCTTCGACCTG
  >scaffold00001_47_48_1044_R
  >scaffold00001_47_48_1044_R
  CACCAATCTGCTTGTGGGTCTGGTGCTGAC
  CACCAATCTGCTTGTGGGTCTGGTGCTGAC
 +
  >scaffold00001_48_49_1639_F
  >scaffold00001_48_49_1639_F
  GATGTTCATCGACCTGGAGAACGTGC
  GATGTTCATCGACCTGGAGAACGTGC
  >scaffold00001_48_49_244_R
  >scaffold00001_48_49_244_R
  CATCTCTCCCGTCAGCTCATCGTTCGAAG
  CATCTCTCCCGTCAGCTCATCGTTCGAAG
 +
  >scaffold00001_49_50_1297_F
  >scaffold00001_49_50_1297_F
  CAGGGTTTCCTGAGCCACCTTGAAC
  CAGGGTTTCCTGAGCCACCTTGAAC
  >scaffold00001_49_50_822_R
  >scaffold00001_49_50_822_R
  CTGTTCTCCCAGGACCCGATCTTCAACTC
  CTGTTCTCCCAGGACCCGATCTTCAACTC
 +
  >scaffold00001_50_51_227_F
  >scaffold00001_50_51_227_F
  CGTGCCATGTTCTGAACGAGAACGATC
  CGTGCCATGTTCTGAACGAGAACGATC
  >scaffold00001_50_51_860_R
  >scaffold00001_50_51_860_R
  GTGACCAGCGAGTGAATCTTGTCGCGTTG
  GTGACCAGCGAGTGAATCTTGTCGCGTTG
 +
  >scaffold00001_55_56_239_F
  >scaffold00001_55_56_239_F
  CACCTGACCTCTGACAATGAACGCCGAAAG
  CACCTGACCTCTGACAATGAACGCCGAAAG
  >scaffold00001_55_56_206_R
  >scaffold00001_55_56_206_R
  GATTGGCTTCTATACAAACTAAGGTGGCGC
  GATTGGCTTCTATACAAACTAAGGTGGCGC
 +
  >scaffold00001_57_58_1204_F
  >scaffold00001_57_58_1204_F
  TCATGCTGTCCGTATCCATTTAGGACCTTC
  TCATGCTGTCCGTATCCATTTAGGACCTTC
  >scaffold00001_57_58_939_R
  >scaffold00001_57_58_939_R
  TTCTACCGTGCCTACTTCGAGACCGAC
  TTCTACCGTGCCTACTTCGAGACCGAC
 +
  >scaffold00001_61_62_285_F
  >scaffold00001_61_62_285_F
  TCAGGTTGAGATCGACCATCGCGAG
  TCAGGTTGAGATCGACCATCGCGAG
  >scaffold00001_61_62_944_R
  >scaffold00001_61_62_944_R
  TTGCACCATCAGCTCAGCATCGTCTTCGAC
  TTGCACCATCAGCTCAGCATCGTCTTCGAC
 +
  >scaffold00001_62_63_898_F
  >scaffold00001_62_63_898_F
  CTGATGATCAAGGTGTTGCGGCTGTC
  CTGATGATCAAGGTGTTGCGGCTGTC
  >scaffold00001_62_63_367_R
  >scaffold00001_62_63_367_R
  GTTGTAGCCGCCGAAGTCGTTCATGCTGAC
  GTTGTAGCCGCCGAAGTCGTTCATGCTGAC
 +
  >scaffold00001_67_68_5122_F
  >scaffold00001_67_68_5122_F
-
  Can't find proper primer
+
  GACCGTTGCGACCAGATATTCGGCGATT
  >scaffold00001_67_68_0_R
  >scaffold00001_67_68_0_R
-
  Can't find proper primer
+
  AACCGCTGACCGCGTTCGTGCTGTTCT
 +
 
  >scaffold00001_71_72_349_F
  >scaffold00001_71_72_349_F
  AACCCACGCATCCCACTCAACGAGCTGAAC
  AACCCACGCATCCCACTCAACGAGCTGAAC
  >scaffold00001_71_72_0_R
  >scaffold00001_71_72_0_R
-
  Can't find proper primer
+
  TCGTTGTCCACCACGCCGACGAACA
 +
 
  >scaffold00001_73_74_2968_F
  >scaffold00001_73_74_2968_F
-
  Can't find proper primer
+
  TACGCGGCAGTCGGACGGGTGTGTT
  >scaffold00001_73_74_0_R
  >scaffold00001_73_74_0_R
-
  Can't find proper primer
+
  GCTCACCGTGCCGGTGAGGACCAA
 +
 
  >scaffold00001_75_76_788_F
  >scaffold00001_75_76_788_F
  GTCGACGTTTCATCACGTCACTGGGTG
  GTCGACGTTTCATCACGTCACTGGGTG
  >scaffold00001_75_76_0_R
  >scaffold00001_75_76_0_R
-
  Can't find proper primer
+
  GTGCGCCAAGACCTGCGCGAACAGT
 +
 
  >scaffold00001_76_77_1854_F
  >scaffold00001_76_77_1854_F
  GTGATCAAGGTGATCGAAGCCCTGC
  GTGATCAAGGTGATCGAAGCCCTGC
  >scaffold00001_76_77_535_R
  >scaffold00001_76_77_535_R
  ATCTACAACCACCTGGGCGGCAAGGAGAAC
  ATCTACAACCACCTGGGCGGCAAGGAGAAC
 +
  >scaffold00001_80_81_426_F
  >scaffold00001_80_81_426_F
  CACTGCCCAAACCGATCTATCCGACGTTC
  CACTGCCCAAACCGATCTATCCGACGTTC
  >scaffold00001_80_81_202_R
  >scaffold00001_80_81_202_R
  GATCCAAGCTGTCTTAACGAGGAGTTGCC
  GATCCAAGCTGTCTTAACGAGGAGTTGCC
 +
  >scaffold00001_87_88_204_F
  >scaffold00001_87_88_204_F
  GAAGTGATCACGCGATCCACGGGATTCCAC
  GAAGTGATCACGCGATCCACGGGATTCCAC
  >scaffold00001_87_88_267_R
  >scaffold00001_87_88_267_R
  AGCTTCCAGACCCTGGTCAACAGTC
  AGCTTCCAGACCCTGGTCAACAGTC
 +
  >scaffold00001_88_89_1973_F
  >scaffold00001_88_89_1973_F
  AGTAGCCGATGACCCCGTTGAGTTG
  AGTAGCCGATGACCCCGTTGAGTTG
  >scaffold00001_88_89_463_R
  >scaffold00001_88_89_463_R
  CAGGTGTTCAACCTCGCCCAGACCTTCTAC
  CAGGTGTTCAACCTCGCCCAGACCTTCTAC
 +
  >scaffold00003_3_4_218_F
  >scaffold00003_3_4_218_F
  ACAAGCTCATTGCCTACTAGATCGACTTCG
  ACAAGCTCATTGCCTACTAGATCGACTTCG
  >scaffold00003_3_4_1054_R
  >scaffold00003_3_4_1054_R
  GCATCGTCCAACGGTAGCCTTCTTTC
  GCATCGTCCAACGGTAGCCTTCTTTC
 +
  >scaffold00003_7_8_365_F
  >scaffold00003_7_8_365_F
  ATCCGACGAGTACGACATCCACCTG
  ATCCGACGAGTACGACATCCACCTG
  >scaffold00003_7_8_601_R
  >scaffold00003_7_8_601_R
  CAGACCAACTACGCCACAAGCAAGG
  CAGACCAACTACGCCACAAGCAAGG
 +
  >scaffold00003_8_9_472_F
  >scaffold00003_8_9_472_F
  CTGTTCCAGACCTTCCTCACCGTGCTTGAC
  CTGTTCCAGACCTTCCTCACCGTGCTTGAC
  >scaffold00003_8_9_218_R
  >scaffold00003_8_9_218_R
  CTTATGCCCTGACAGAAGGTGCAAGATCAG
  CTTATGCCCTGACAGAAGGTGCAAGATCAG
 +
  >scaffold00003_10_11_1436_F
  >scaffold00003_10_11_1436_F
  TGGTCCTGCCGTACTTCCTGATCACCACTG
  TGGTCCTGCCGTACTTCCTGATCACCACTG
  >scaffold00003_10_11_232_R
  >scaffold00003_10_11_232_R
  GATGGGACAGACTTCGTACACCGGTTG
  GATGGGACAGACTTCGTACACCGGTTG
 +
  >scaffold00003_11_12_266_F
  >scaffold00003_11_12_266_F
  CTGTATTGATGATCCACTCGCTGCCGCTC
  CTGTATTGATGATCCACTCGCTGCCGCTC
  >scaffold00003_11_12_1184_R
  >scaffold00003_11_12_1184_R
  TACAGTGATCGGCCGTTGTTGGAGTGTGTG
  TACAGTGATCGGCCGTTGTTGGAGTGTGTG
 +
  >scaffold00003_17_18_314_F
  >scaffold00003_17_18_314_F
  TCTGAACGAGACGGAATGGGAGATCCTCAC
  TCTGAACGAGACGGAATGGGAGATCCTCAC
  >scaffold00003_17_18_222_R
  >scaffold00003_17_18_222_R
  GACATCACCATCCAGGCAGAGAACATGCTG
  GACATCACCATCCAGGCAGAGAACATGCTG
 +
  >scaffold00003_19_20_655_F
  >scaffold00003_19_20_655_F
  GAAGGAACGGCAACTTTCGGTCACGGTAC
  GAAGGAACGGCAACTTTCGGTCACGGTAC
  >scaffold00003_19_20_485_R
  >scaffold00003_19_20_485_R
  CAAGGAGACCGTCTACAAGATCTGGCATC
  CAAGGAGACCGTCTACAAGATCTGGCATC
 +
  >scaffold00003_23_24_235_F
  >scaffold00003_23_24_235_F
  CCCAGGGAATATAACGATGGTTCAGGTCAG
  CCCAGGGAATATAACGATGGTTCAGGTCAG
  >scaffold00003_23_24_1163_R
  >scaffold00003_23_24_1163_R
  GAACTGCTGGTTCATCGAATGGTCGTTGAC
  GAACTGCTGGTTCATCGAATGGTCGTTGAC
 +
  >scaffold00003_24_25_268_F
  >scaffold00003_24_25_268_F
  CTCGAAGTCCCGGTCAAGAAGATCCTCAC
  CTCGAAGTCCCGGTCAAGAAGATCCTCAC
  >scaffold00003_24_25_666_R
  >scaffold00003_24_25_666_R
  TATCGTTCGCGTCGATGGCCGACAACATTG
  TATCGTTCGCGTCGATGGCCGACAACATTG
 +
  >scaffold00003_26_27_1270_F
  >scaffold00003_26_27_1270_F
  GAAATCAAGATCCTGAACCAGTCCCGGAAG
  GAAATCAAGATCCTGAACCAGTCCCGGAAG
  >scaffold00003_26_27_244_R
  >scaffold00003_26_27_244_R
  CCCGATTCCTTGTCCTGGAACAGATAGAAG
  CCCGATTCCTTGTCCTGGAACAGATAGAAG
 +
  >scaffold00003_27_28_833_F
  >scaffold00003_27_28_833_F
  GTACTCGCCGACTTTCCCGTTCTCATC
  GTACTCGCCGACTTTCCCGTTCTCATC
  >scaffold00003_27_28_474_R
  >scaffold00003_27_28_474_R
  GTACACCTCGTGCTCGGGACACAAATGGTC
  GTACACCTCGTGCTCGGGACACAAATGGTC
 +
  >scaffold00003_28_29_210_F
  >scaffold00003_28_29_210_F
  GTACACGTGCCGTTCCCGTTTCTGGTGAAC
  GTACACGTGCCGTTCCCGTTTCTGGTGAAC
  >scaffold00003_28_29_173_R
  >scaffold00003_28_29_173_R
  ACGGCGTCACGAACAACACCTACTG
  ACGGCGTCACGAACAACACCTACTG
 +
  >scaffold00003_30_31_256_F
  >scaffold00003_30_31_256_F
  TTCCGGGGATGAGTAGCCGACCAATTGTCC
  TTCCGGGGATGAGTAGCCGACCAATTGTCC
  >scaffold00003_30_31_1239_R
  >scaffold00003_30_31_1239_R
  GATCACCGGTTGGTAGACCAGATCCAG
  GATCACCGGTTGGTAGACCAGATCCAG
 +
  >scaffold00003_31_32_351_F
  >scaffold00003_31_32_351_F
  CACAAATTGAACTTGAGCTGGGATGTCAGG
  CACAAATTGAACTTGAGCTGGGATGTCAGG
  >scaffold00003_31_32_565_R
  >scaffold00003_31_32_565_R
  CAGGATGACAGTGTGAGTCAGCCATGGAC
  CAGGATGACAGTGTGAGTCAGCCATGGAC
 +
  >scaffold00003_35_36_707_F
  >scaffold00003_35_36_707_F
  AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
  AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
  >scaffold00003_35_36_243_R
  >scaffold00003_35_36_243_R
  CAGGTTGAACATCTCCTGGACCAGCGAAC
  CAGGTTGAACATCTCCTGGACCAGCGAAC
 +
  >scaffold00003_42_43_317_F
  >scaffold00003_42_43_317_F
  GTTCCTGTCCTCGTGCAGCACAATTCGATC
  GTTCCTGTCCTCGTGCAGCACAATTCGATC
  >scaffold00003_42_43_298_R
  >scaffold00003_42_43_298_R
  ACCGGGGATCATCGTGGTCTTGTAC
  ACCGGGGATCATCGTGGTCTTGTAC
 +
  >scaffold00003_43_44_737_F
  >scaffold00003_43_44_737_F
  ATCGTTCGCAGCGGCATCGATTCAC
  ATCGTTCGCAGCGGCATCGATTCAC
  >scaffold00003_43_44_992_R
  >scaffold00003_43_44_992_R
  TGAAGCTGACCAAGCTGGTGGTCTC
  TGAAGCTGACCAAGCTGGTGGTCTC
 +
  >scaffold00003_45_46_422_F
  >scaffold00003_45_46_422_F
  GTGATGCCCTCGGATCCCAGTTCTTCGTAC
  GTGATGCCCTCGGATCCCAGTTCTTCGTAC
  >scaffold00003_45_46_399_R
  >scaffold00003_45_46_399_R
  AACTCGGTCAAGCTCGAGTTCCTGGTG
  AACTCGGTCAAGCTCGAGTTCCTGGTG
 +
  >scaffold00003_47_48_427_F
  >scaffold00003_47_48_427_F
  CATCACGGCGAACAACAACCAGACCTAC
  CATCACGGCGAACAACAACCAGACCTAC
  >scaffold00003_47_48_271_R
  >scaffold00003_47_48_271_R
  GAACAACGGCATGAGGTCCTTGAACATGAC
  GAACAACGGCATGAGGTCCTTGAACATGAC
 +
  >scaffold00003_48_49_532_F
  >scaffold00003_48_49_532_F
  GAAGGGTCAACAGTGAGATCGCCTGGTTC
  GAAGGGTCAACAGTGAGATCGCCTGGTTC
  >scaffold00003_48_49_741_R
  >scaffold00003_48_49_741_R
  TGTAGCTGCCCTTCTCCTCCTGTTC
  TGTAGCTGCCCTTCTCCTCCTGTTC
 +
  >scaffold00003_49_50_208_F
  >scaffold00003_49_50_208_F
  GTGGTGTGCATCGTGCAGATCCCGTTCTAC
  GTGGTGTGCATCGTGCAGATCCCGTTCTAC
  >scaffold00003_49_50_975_R
  >scaffold00003_49_50_975_R
  AACCCGGAGCCGAAGAGTTCGAGAAAC
  AACCCGGAGCCGAAGAGTTCGAGAAAC
 +
  >scaffold00003_50_51_1445_F
  >scaffold00003_50_51_1445_F
  TGGCGTACCTGGAGAAGGTGGTTTC
  TGGCGTACCTGGAGAAGGTGGTTTC
  >scaffold00003_50_51_439_R
  >scaffold00003_50_51_439_R
  CAATGGCACCCTCACATATGGCAGATAATTG
  CAATGGCACCCTCACATATGGCAGATAATTG
 +
  >scaffold00004_8_9_447_F
  >scaffold00004_8_9_447_F
  GAACCTGCTGTTCATGGTCGGTCTG
  GAACCTGCTGTTCATGGTCGGTCTG
  >scaffold00004_8_9_234_R
  >scaffold00004_8_9_234_R
  ACAGTGGTCTCCGTCTACAGTCCAC
  ACAGTGGTCTCCGTCTACAGTCCAC
 +
  >scaffold00004_12_13_750_F
  >scaffold00004_12_13_750_F
  TCGATGGACATGCTCTCCTCCGAGATCGTC
  TCGATGGACATGCTCTCCTCCGAGATCGTC
  >scaffold00004_12_13_895_R
  >scaffold00004_12_13_895_R
  TATGCGAGCGTCCGAAAAAGATCGACCAGC
  TATGCGAGCGTCCGAAAAAGATCGACCAGC
 +
  >scaffold00004_13_14_550_F
  >scaffold00004_13_14_550_F
  GAGCACATCAAGATCACTGACAATGGGATC
  GAGCACATCAAGATCACTGACAATGGGATC
  >scaffold00004_13_14_243_R
  >scaffold00004_13_14_243_R
  AACACTCCGAAATCCGCTGGCTCTC
  AACACTCCGAAATCCGCTGGCTCTC
 +
  >scaffold00004_14_15_556_F
  >scaffold00004_14_15_556_F
  CTGGTGGGACTCGTTGAGCATCCAATG
  CTGGTGGGACTCGTTGAGCATCCAATG
  >scaffold00004_14_15_220_R
  >scaffold00004_14_15_220_R
  CCGAGGGAAAGAACCGGACATTGTG
  CCGAGGGAAAGAACCGGACATTGTG
 +
  >scaffold00004_15_16_1515_F
  >scaffold00004_15_16_1515_F
  CTTCACCGGCCTGTTCTTCGTCATCAC
  CTTCACCGGCCTGTTCTTCGTCATCAC
  >scaffold00004_15_16_167_R
  >scaffold00004_15_16_167_R
  CAGTACCTGTGGCGTCACACGCGATAAGAG
  CAGTACCTGTGGCGTCACACGCGATAAGAG
 +
  >scaffold00004_16_17_236_F
  >scaffold00004_16_17_236_F
  GGTTCGACACCAGGATTTCGAGGTCAC
  GGTTCGACACCAGGATTTCGAGGTCAC
  >scaffold00004_16_17_467_R
  >scaffold00004_16_17_467_R
  GATCTGGTTCCACATCTGTCCCTGGTG
  GATCTGGTTCCACATCTGTCCCTGGTG
 +
  >scaffold00004_18_19_226_F
  >scaffold00004_18_19_226_F
  GTGTGATCGACACGGTTACGTCGAAG
  GTGTGATCGACACGGTTACGTCGAAG
  >scaffold00004_18_19_492_R
  >scaffold00004_18_19_492_R
  TTAACAGGGGGCCCTTCCTTCAACACGGTC
  TTAACAGGGGGCCCTTCCTTCAACACGGTC
 +
  >scaffold00004_20_21_2075_F
  >scaffold00004_20_21_2075_F
  AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
  AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
  >scaffold00004_20_21_521_R
  >scaffold00004_20_21_521_R
  TGAAGATCACCCAGGATCTCAAGGAGTACG
  TGAAGATCACCCAGGATCTCAAGGAGTACG
 +
  >scaffold00004_21_22_386_F
  >scaffold00004_21_22_386_F
  GAAGACTTCGCCACCGAATACCTCAGC
  GAAGACTTCGCCACCGAATACCTCAGC
  >scaffold00004_21_22_182_R
  >scaffold00004_21_22_182_R
  TTCGACGTACACACCACCCTCGCCGTAATC
  TTCGACGTACACACCACCCTCGCCGTAATC
 +
  >scaffold00004_23_24_720_F
  >scaffold00004_23_24_720_F
  TCCACCGGAACATGGGTGATACTCGAC
  TCCACCGGAACATGGGTGATACTCGAC
  >scaffold00004_23_24_552_R
  >scaffold00004_23_24_552_R
  GTTGCCTTTGCACAGGTAGATCCCG
  GTTGCCTTTGCACAGGTAGATCCCG
 +
  >scaffold00004_24_25_667_F
  >scaffold00004_24_25_667_F
  CAAGCCATGTCGAAGCAGCACTACTC
  CAAGCCATGTCGAAGCAGCACTACTC
  >scaffold00004_24_25_322_R
  >scaffold00004_24_25_322_R
  AAGCACCTGCTGTTCACAAGCGAACTGATC
  AAGCACCTGCTGTTCACAAGCGAACTGATC
 +
  >scaffold00004_25_26_542_F
  >scaffold00004_25_26_542_F
  GCAGATCGACGAGTACCTGGAGTTCTAC
  GCAGATCGACGAGTACCTGGAGTTCTAC
  >scaffold00004_25_26_247_R
  >scaffold00004_25_26_247_R
  AGGAGTCCGACCATGAAGATCAGGAAGATC
  AGGAGTCCGACCATGAAGATCAGGAAGATC
 +
  >scaffold00004_29_30_278_F
  >scaffold00004_29_30_278_F
  CATTTGTGACACCTGTCCAGCAGTACG
  CATTTGTGACACCTGTCCAGCAGTACG
Line 345: Line 424:
  TCTGGTGAGCCGACAGGTATTCCTC
  TCTGGTGAGCCGACAGGTATTCCTC
-
===primer part 2 - scaffold00003, scaffold00004===
+
>scf1_10_11_191_R
 +
ATTCCTCGCGCAGTTGACCCAGTTC
 +
>scf1_10_11_533_F
 +
TTCGCCACCACCGGTCTAGGATGAGAGATC
 +
 +
>scf1_13_14_1221_R
 +
AGTCGTCGAAGCGCAGAAGGTACATGTAGG
 +
>scf1_13_14_961_F
 +
GTCATCGGACAGCCGATTCTTCGACGACTG
-
  >scaffold00003_1_2_276_F
+
  >scf1_14_15_1407_F
-
  GACGTGTACATCTCGAACATCTCGCGG
+
  CTTGTTTCGATAGCATTACCGCCCAGTAAC
-
  >scaffold00003_1_2_387_R
+
  >scf1_14_15_533_R
-
  AAAACGGGCGTTCACCGCATGGTAC
+
  GTACTCGTCGAAGGTGGACACGTTCACTC
-
  >scaffold00003_2_3_232_F
+
 
-
  CAAGGGAGAGTTCCGTTACGACCACAG
+
  >scf1_16_17_1785_F
-
  >scaffold00003_2_3_460_R
+
  CTCGAAGACGTCAACAACCACACCAATCTG
-
  TTGATCAGCCGGCACAGCAACAAGGTGGTG
+
  >scf1_16_17_478_R
-
  >scaffold00003_3_4_218_F
+
  CTCGTAACTCTCCATCGGTTCTCCAGTAAC
-
  ACAAGCTCATTGCCTACTAGATCGACTTCG
+
 
-
  >scaffold00003_3_4_1054_R
+
  >scf1_22_23_238_F
-
  GCATCGTCCAACGGTAGCCTTCTTTC
+
  GTTTCACTCATCGGTGTACCCTCCCGATC
-
  >scaffold00003_4_5_574_F
+
  >scf1_22_23_244_R
-
  ATCGACCTGGTGCACGCGAACAACTCCAAG
+
  GAAGTTCGTCATCGAGAACGTCTCCGTGC
-
  >scaffold00003_4_5_289_R
+
 
-
  GAAGAAGGCGTTGGAGTGCCACAGTTG
+
  >scf1_24_25_444_F
-
  >scaffold00003_5_6_195_F
+
  ATTCGACGGGCAGGACGTCAACAAG
-
  ATCTGTTGCCGAATTGGCCTGTACTAGATC
+
  >scf1_24_25_458_R
-
  >scaffold00003_5_6_263_R
+
  TAGGTGTGCTCCAGGTAGGTGAGGAAC
-
  ATGGCATCCCTCAACCACTACCACC
+
 
-
  >scaffold00003_6_7_918_F
+
  >scf1_26_27_369_F
-
  ATGCTCAGTCCGGGAGTACAGCTCGGTAAC
+
  ATGAGCGAGCAGGTCTTCCTCCTAC
-
  >scaffold00003_6_7_245_R
+
  >scf1_26_27_706_R
-
  GCATGCTGTTGTTGATGCTGTCCTTCAG
+
  GAACACGCCGTAGTTGTTCAGCGTGAAG
-
  >scaffold00003_7_8_365_F
+
 
-
  ATCCGACGAGTACGACATCCACCTG
+
  >scf1_29_30_1643_F
-
  >scaffold00003_7_8_601_R
+
  CCGATCCAGGCGAAGATCAGATTGGTC
-
  CAGACCAACTACGCCACAAGCAAGG
+
  >scf1_29_30_767_R
-
  >scaffold00003_8_9_472_F
+
  ATCGACATAGCGCTCCAACAGATCGGTGAC
-
  CTGTTCCAGACCTTCCTCACCGTGCTTGAC
+
 
-
  >scaffold00003_8_9_218_R
+
  >scf1_32_33_169_R
-
  CTTATGCCCTGACAGAAGGTGCAAGATCAG
+
  TTGTCAATGTAGCGTTGACGGGTGCCTGAC
-
  >scaffold00003_9_10_376_F
+
  >scf1_32_33_266_F
-
  CATGCACAAGCAGGACCTCATCGAG
+
  AACATTGACAGGGTGGCACGCAGTTCG
-
  >scaffold00003_9_10_212_R
+
 
-
  TTTCCGTTGAGCCGCCACTCGTACTTGTAG
+
  >scf1_33_34_365_R
-
  >scaffold00003_10_11_1436_F
+
  GTTCCCTGCTGCTGAACTGGTTCTTCAC
-
  TGGTCCTGCCGTACTTCCTGATCACCACTG
+
  >scf1_33_34_795_F
-
  >scaffold00003_10_11_232_R
+
  GTCGACGACGAGAATCCGGTTCATG
-
  GATGGGACAGACTTCGTACACCGGTTG
+
 
-
  >scaffold00003_11_12_266_F
+
  >scf1_37_38_498_F
-
  CTGTATTGATGATCCACTCGCTGCCGCTC
+
  TTCGACGAACTGTGGCAACGCTTCTCC
-
  >scaffold00003_11_12_1184_R
+
  >scf1_37_38_562_R
-
  TACAGTGATCGGCCGTTGTTGGAGTGTGTG
+
  AGGACGTCATGCACGGTCAACACAC
-
  >scaffold00003_12_13_2070_F
+
 
 +
  >scf1_40_41_522_F
 +
  GACTACAAGCCGGAGAACGTGCTCATCTC
 +
  >scf1_40_41_660_R
 +
  GTGCAGAAGGGATAGAGGGTGACGTAC
 +
 
 +
  >scf1_43_44_440_F
 +
  CTCGGAGGAAAACGGTGAACAGTCAGCAAC
 +
  >scf1_43_44_563_R
 +
  TGAACGCCTCCACCCAGCATTTGATCACAG
 +
 
 +
>scf1_44_45_485_R
 +
GTGTTGCGATCGGATTCGTAGCTGAC
 +
>scf1_44_45_93801_F
 +
ACACTGCTGCCGCCACGGTTGACTGT
 +
 
 +
>scf1_46_47_370_F
 +
GTAAACTCCTGATCTCAGCCGCTCTCGTG
 +
>scf1_46_47_845_R
 +
CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG
 +
 
 +
>scf1_53_54_414_F
 +
TTCTGGAAGACCATCTGCAGACCGATCTCG
 +
>scf1_53_54_823_R
 +
TACGCGAAGGGACGTTAGAGATGGCAC
 +
 
 +
>scf1_54_55_1250_F
 +
CTGGAACCGGATGATGTTGGAGTCCTC
 +
>scf1_54_55_175_R
 +
TACCGAGGAACTGATCGACCTCTCC
 +
 
 +
>scf1_56_57_184_R
 +
CAAACCGGTGGTCGGGCTGATCATGTATTC
 +
>scf1_56_57_412_F
 +
GTACTCACGATGGTCCTTCTCCTCGTTG
 +
 
 +
>scf1_59_60_1979_F
 +
GTGACCAGCCAGGTCTGTTCGAATCTCAC
 +
>scf1_59_60_625_R
 +
GAGATCATGTACGCCGACTTCAGCTTCGTG
 +
 
 +
>scf1_63_64_1286_R
 +
CGATAGGTGAGACTCGATGCATGGTTAGTG
 +
>scf1_63_64_711_F
 +
ACGTAGTTCCCGACCGTAGAGCAGTTC
 +
 
 +
>scf1_66_67_346_R
 +
ACTGTTCCTGCTGTACTCCTCGGTG
 +
>scf1_66_67_428_F
 +
CTTCGTCACTGGCTGTGTCAAGGTCAAC
 +
 
 +
>scf1_6_7_870_R
 +
CGATCTCCACGTTTGACAGGATGCTCTG
 +
>scf1_6_7_939_F
 +
ATCAGCGGTTACGTCTGGTGGTGAC
 +
 
 +
>scf1_7_8_545_R
 +
ATCAGTTTGATCGGCGAGACGTGCGAGACC
 +
>scf1_7_8_818_F
 +
GAACTGGAGTTGATGGAGCAGCGCGAAAC
 +
 
 +
  >scf3_12_13_2070_F
  CTCCAGCAGGGTTTCATCGATTCGGTAG
  CTCCAGCAGGGTTTCATCGATTCGGTAG
-
  >scaffold00003_12_13_324_R
+
  >scf3_12_13_324_R
  CTGGACCAGTTCACCCTCCAGATCATG
  CTGGACCAGTTCACCCTCCAGATCATG
-
>scaffold00003_13_14_463_F
+
 
-
CTGGTGTCACACAACGAAAAGGACCTGAC
+
  >scf3_18_19_471_R
-
>scaffold00003_13_14_304_R
+
  GTATACCGCAACATCGGCGAAATGAAGTC
-
GAAGAAGTCGTCCTTCTCCTTGACCGACAG
+
  >scf3_18_19_746_F
-
>scaffold00003_14_15_137305_F
+
-
Can't find proper primer
+
-
>scaffold00003_14_15_518_R
+
-
AATACCGCTACGGTGCTGGAACCGATGTAC
+
-
>scaffold00003_15_16_227_F
+
-
GTTTCTGGACAAGTACGGTTTCGGGACC
+
-
>scaffold00003_15_16_170_R
+
-
CTTGAGGCGCTCACTGATGCACTTAAC
+
-
>scaffold00003_16_17_319_F
+
-
TCACGTGATCTCCTGATAGGTAGACAGACG
+
-
>scaffold00003_16_17_503_R
+
-
TACCTGTGGCCCCAAAGCTACTACCTG
+
-
>scaffold00003_17_18_314_F
+
-
TCTGAACGAGACGGAATGGGAGATCCTCAC
+
-
  >scaffold00003_17_18_222_R
+
-
  GACATCACCATCCAGGCAGAGAACATGCTG
+
-
  >scaffold00003_18_19_746_F
+
  GTTGCTTCGAATCTGGTGACGGGAG
  GTTGCTTCGAATCTGGTGACGGGAG
-
>scaffold00003_18_19_471_R
+
 
-
GTATACCGCAACATCGGCGAAATGAAGTC
+
  >scf3_20_21_200_R
-
>scaffold00003_19_20_655_F
+
  GAAGATCGGCAGCTCCATCGTCTCATC
-
GAAGGAACGGCAACTTTCGGTCACGGTAC
+
  >scf3_20_21_294_F
-
  >scaffold00003_19_20_485_R
+
-
  CAAGGAGACCGTCTACAAGATCTGGCATC
+
-
  >scaffold00003_20_21_294_F
+
  ATCAGCCCCGAGCAATTGACCGACCTGAAC
  ATCAGCCCCGAGCAATTGACCGACCTGAAC
-
>scaffold00003_20_21_200_R
+
 
-
GAAGATCGGCAGCTCCATCGTCTCATC
+
  >scf3_21_22_1034_F
-
  >scaffold00003_21_22_1034_F
+
  GAAGTAACCGGTGGTGTTTTCGGCCAG
  GAAGTAACCGGTGGTGTTTTCGGCCAG
-
  >scaffold00003_21_22_354_R
+
  >scf3_21_22_354_R
  ATCTCGCCGGTTACAAGCAGATCCTC
  ATCTCGCCGGTTACAAGCAGATCCTC
-
  >scaffold00003_22_23_1299_F
+
 
 +
  >scf3_22_23_1299_F
  TTCAGATGGTTAAGGACAGCTTCATGAGCG
  TTCAGATGGTTAAGGACAGCTTCATGAGCG
-
  >scaffold00003_22_23_368_R
+
  >scf3_22_23_368_R
  ACGTGATCGGTGGGAACTTCGACTC
  ACGTGATCGGTGGGAACTTCGACTC
-
>scaffold00003_23_24_235_F
+
 
-
CCCAGGGAATATAACGATGGTTCAGGTCAG
+
  >scf3_25_26_360_F
-
>scaffold00003_23_24_1163_R
+
-
GAACTGCTGGTTCATCGAATGGTCGTTGAC
+
-
>scaffold00003_24_25_268_F
+
-
CTCGAAGTCCCGGTCAAGAAGATCCTCAC
+
-
>scaffold00003_24_25_666_R
+
-
TATCGTTCGCGTCGATGGCCGACAACATTG
+
-
  >scaffold00003_25_26_360_F
+
  GAAAGGCTGACCATCTACCCGGAGTAC
  GAAAGGCTGACCATCTACCCGGAGTAC
-
  >scaffold00003_25_26_636_R
+
  >scf3_25_26_636_R
  TGATCCACGTGTCCGTACATCATCGTG
  TGATCCACGTGTCCGTACATCATCGTG
-
>scaffold00003_26_27_1270_F
+
 
-
GAAATCAAGATCCTGAACCAGTCCCGGAAG
+
  >scf3_33_34_1969_F
-
>scaffold00003_26_27_244_R
+
-
CCCGATTCCTTGTCCTGGAACAGATAGAAG
+
-
>scaffold00003_27_28_833_F
+
-
GTACTCGCCGACTTTCCCGTTCTCATC
+
-
>scaffold00003_27_28_474_R
+
-
GTACACCTCGTGCTCGGGACACAAATGGTC
+
-
>scaffold00003_28_29_210_F
+
-
GTACACGTGCCGTTCCCGTTTCTGGTGAAC
+
-
>scaffold00003_28_29_173_R
+
-
ACGGCGTCACGAACAACACCTACTG
+
-
>scaffold00003_29_30_703_F
+
-
TCAGCGATTCAGAGGTCCCGATCAGAGGTC
+
-
>scaffold00003_29_30_1341_R
+
-
TACCTGCATACCGTACGTGGAGTCG
+
-
>scaffold00003_30_31_256_F
+
-
TTCCGGGGATGAGTAGCCGACCAATTGTCC
+
-
>scaffold00003_30_31_1239_R
+
-
GATCACCGGTTGGTAGACCAGATCCAG
+
-
>scaffold00003_31_32_351_F
+
-
CACAAATTGAACTTGAGCTGGGATGTCAGG
+
-
>scaffold00003_31_32_565_R
+
-
CAGGATGACAGTGTGAGTCAGCCATGGAC
+
-
>scaffold00003_32_33_376_F
+
-
AGATCGACGAGCTCAACGTCTATCCG
+
-
>scaffold00003_32_33_1069_R
+
-
GTCATTCGACACCCACCAGCAACGGATAG
+
-
  >scaffold00003_33_34_1969_F
+
  GTCCTCAAGCATCGCAAGCGCAAGGAAAAG
  GTCCTCAAGCATCGCAAGCGCAAGGAAAAG
-
  >scaffold00003_33_34_485_R
+
  >scf3_33_34_485_R
  CTCCAACTGCTCGGAGAGGAACTTGTAAC
  CTCCAACTGCTCGGAGAGGAACTTGTAAC
-
  >scaffold00003_34_35_491_F
+
 
 +
  >scf3_34_35_222_R
 +
ATCTCCGCAGTCATATCGGTACGGG
 +
>scf3_34_35_491_F
  GAACGAGACCAGGAGACATAGTGGCGAGTC
  GAACGAGACCAGGAGACATAGTGGCGAGTC
-
>scaffold00003_34_35_222_R
+
 
-
ATCTCCGCAGTCATATCGGTACGGG
+
  >scf3_36_37_355_F
-
>scaffold00003_35_36_707_F
+
-
AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
+
-
>scaffold00003_35_36_243_R
+
-
CAGGTTGAACATCTCCTGGACCAGCGAAC
+
-
  >scaffold00003_36_37_355_F
+
  CTGCTCTTCCTCGGCATGCTCATGTTC
  CTGCTCTTCCTCGGCATGCTCATGTTC
-
  >scaffold00003_36_37_430_R
+
  >scf3_36_37_430_R
  ATAGCCCGCCCACTGCATGACAAAC
  ATAGCCCGCCCACTGCATGACAAAC
-
>scaffold00003_37_38_542_F
+
 
-
CGACTGGGAGTACTACATCGGTAACACC
+
  >scf3_38_39_613_R
-
>scaffold00003_37_38_565_R
+
-
GCACTGCGTGAACTCCCTTTCCATC
+
-
>scaffold00003_38_39_791_F
+
-
AACTGTCGACGAAGAACCGCGTCATGAGAG
+
-
  >scaffold00003_38_39_613_R
+
  GTACGAGCAGTTCACCAGTTGCGTG
  GTACGAGCAGTTCACCAGTTGCGTG
-
  >scaffold00003_39_40_351_F
+
  >scf3_38_39_791_F
 +
AACTGTCGACGAAGAACCGCGTCATGAGAG
 +
 
 +
>scf3_39_40_333_R
 +
CTGATCCGTCATGGCGCACATTCCACGATG
 +
>scf3_39_40_351_F
  CACTTGTCTTCTCCTCCGGATACCTGG
  CACTTGTCTTCTCCTCCGGATACCTGG
-
>scaffold00003_39_40_333_R
+
 
-
CTGATCCGTCATGGCGCACATTCCACGATG
+
  >scf3_44_45_1117_F
-
>scaffold00003_40_41_416_F
+
-
GTGGTAGGTCTGTGCCTGGTGGTTATG
+
-
>scaffold00003_40_41_370_R
+
-
AAGTAGTGCGGCTCCTGCCATCTTCGTTTG
+
-
>scaffold00003_41_42_419_F
+
-
GTAGGTCTGTGCCTGGTGCTGGTGGTTATG
+
-
>scaffold00003_41_42_166_R
+
-
GTACCGCCAACACGACGACTCATTC
+
-
>scaffold00003_42_43_317_F
+
-
GTTCCTGTCCTCGTGCAGCACAATTCGATC
+
-
>scaffold00003_42_43_298_R
+
-
ACCGGGGATCATCGTGGTCTTGTAC
+
-
>scaffold00003_43_44_737_F
+
-
ATCGTTCGCAGCGGCATCGATTCAC
+
-
>scaffold00003_43_44_992_R
+
-
TGAAGCTGACCAAGCTGGTGGTCTC
+
-
  >scaffold00003_44_45_1117_F
+
  CTCTGTGAGTACGTGGAACAGGCGATG
  CTCTGTGAGTACGTGGAACAGGCGATG
-
  >scaffold00003_44_45_224_R
+
  >scf3_44_45_224_R
  AAGATCACCACGGCGTCGACACAGCTCAAC
  AAGATCACCACGGCGTCGACACAGCTCAAC
-
>scaffold00003_45_46_422_F
+
 
-
GTGATGCCCTCGGATCCCAGTTCTTCGTAC
+
  >scf3_6_7_245_R
-
  >scaffold00003_45_46_399_R
+
  GCATGCTGTTGTTGATGCTGTCCTTCAG
-
  AACTCGGTCAAGCTCGAGTTCCTGGTG
+
  >scf3_6_7_918_F
-
  >scaffold00003_46_47_258_F
+
  ATGCTCAGTCCGGGAGTACAGCTCGGTAAC
-
  GACCTGCTGAGTGGATCATCAACCTATCG
+
 
-
>scaffold00003_46_47_510_R
+
  >scf3_9_10_212_R
-
GTTGTAACCCATGGCTGACGAAGTAGGG
+
  TTTCCGTTGAGCCGCCACTCGTACTTGTAG
-
  >scaffold00003_47_48_427_F
+
  >scf3_9_10_376_F
-
  CATCACGGCGAACAACAACCAGACCTAC
+
  CATGCACAAGCAGGACCTCATCGAG
-
  >scaffold00003_47_48_271_R
+
 
-
  GAACAACGGCATGAGGTCCTTGAACATGAC
+
  >scf4_19_20_273_F
-
>scaffold00003_48_49_532_F
+
-
GAAGGGTCAACAGTGAGATCGCCTGGTTC
+
-
>scaffold00003_48_49_741_R
+
-
TGTAGCTGCCCTTCTCCTCCTGTTC
+
-
>scaffold00003_49_50_208_F
+
-
GTGGTGTGCATCGTGCAGATCCCGTTCTAC
+
-
>scaffold00003_49_50_975_R
+
-
AACCCGGAGCCGAAGAGTTCGAGAAAC
+
-
>scaffold00003_50_51_1445_F
+
-
TGGCGTACCTGGAGAAGGTGGTTTC
+
-
>scaffold00003_50_51_439_R
+
-
CAATGGCACCCTCACATATGGCAGATAATTG
+
-
>scaffold00004_1_2_381_F
+
-
GAACACGTCACAACCGTGCTTCCAG
+
-
>scaffold00004_1_2_1879_R
+
-
ATCGTCTGGGTCAACACCTGGTTCCTG
+
-
>scaffold00004_2_3_250_F
+
-
GTTGAACCGTCTGAACGACCGAGGAAG
+
-
>scaffold00004_2_3_411_R
+
-
TGTTGTCGGGGTAGGTGGGTAAACG
+
-
>scaffold00004_3_4_333_F
+
-
TGACTTCTCCGACCAGATCGCACACCTGTC
+
-
>scaffold00004_3_4_675_R
+
-
GAGCTTCTCCTTGTGCTGGGTCAAC
+
-
>scaffold00004_4_5_297_F
+
-
GGGAGGTTGTAGATCACGAACTGGATGTAG
+
-
>scaffold00004_4_5_180_R
+
-
CAGAACGAGTCCTACAACGGGAACCTTTC
+
-
>scaffold00004_5_6_411_F
+
-
GAGATCATGTCGCTGGGCACCAATC
+
-
>scaffold00004_5_6_469_R
+
-
AGCAGAGCACCCGTCTGATGTACAG
+
-
>scaffold00004_6_7_257_F
+
-
CGCGTTGAAAAACCGGGCGATATCC
+
-
>scaffold00004_6_7_545_R
+
-
GTCTTCGTGGTCAACAAGGCCGATC
+
-
>scaffold00004_7_8_584_F
+
-
TGATCAACGAGGCGTGCAACGGTAG
+
-
>scaffold00004_7_8_419_R
+
-
ATGATCGACACCGTTACGGCGAGATGG
+
-
>scaffold00004_8_9_447_F
+
-
GAACCTGCTGTTCATGGTCGGTCTG
+
-
>scaffold00004_8_9_234_R
+
-
ACAGTGGTCTCCGTCTACAGTCCAC
+
-
>scaffold00004_9_10_1726_F
+
-
TAGAGGGTCAACGGTTCGTCACCAG
+
-
>scaffold00004_9_10_313_R
+
-
GTCAAGGATGACTGCGTGACTGGCTAC
+
-
>scaffold00004_10_11_783_F
+
-
AGAGCAGTTCGAGGATGTTGGGGTC
+
-
>scaffold00004_10_11_256_R
+
-
GCCGACTTCTACGTACTGCTGTCCATG
+
-
>scaffold00004_11_12_1607_F
+
-
TACCGACAAGTGAGAGGACGACGTG
+
-
>scaffold00004_11_12_704_R
+
-
GACCTGATCGCGGTGATTGGTTGAC
+
-
>scaffold00004_12_13_750_F
+
-
TCGATGGACATGCTCTCCTCCGAGATCGTC
+
-
>scaffold00004_12_13_895_R
+
-
TATGCGAGCGTCCGAAAAAGATCGACCAGC
+
-
>scaffold00004_13_14_550_F
+
-
GAGCACATCAAGATCACTGACAATGGGATC
+
-
>scaffold00004_13_14_243_R
+
-
AACACTCCGAAATCCGCTGGCTCTC
+
-
>scaffold00004_14_15_556_F
+
-
CTGGTGGGACTCGTTGAGCATCCAATG
+
-
>scaffold00004_14_15_220_R
+
-
CCGAGGGAAAGAACCGGACATTGTG
+
-
>scaffold00004_15_16_1515_F
+
-
CTTCACCGGCCTGTTCTTCGTCATCAC
+
-
>scaffold00004_15_16_167_R
+
-
CAGTACCTGTGGCGTCACACGCGATAAGAG
+
-
>scaffold00004_16_17_236_F
+
-
GGTTCGACACCAGGATTTCGAGGTCAC
+
-
>scaffold00004_16_17_467_R
+
-
GATCTGGTTCCACATCTGTCCCTGGTG
+
-
>scaffold00004_17_18_420_F
+
-
CCTGAGGCACAAGTCAGGGTATCGATC
+
-
>scaffold00004_17_18_720_R
+
-
CCAACATCAAGAGCCACCACAACGTC
+
-
>scaffold00004_18_19_226_F
+
-
GTGTGATCGACACGGTTACGTCGAAG
+
-
>scaffold00004_18_19_492_R
+
-
TTAACAGGGGGCCCTTCCTTCAACACGGTC
+
-
  >scaffold00004_19_20_273_F
+
  GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC
  GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC
-
  >scaffold00004_19_20_878_R
+
  >scf4_19_20_878_R
  GACGAGCAACTGGACTCGCTGAACAAG
  GACGAGCAACTGGACTCGCTGAACAAG
-
>scaffold00004_20_21_2075_F
+
 
-
AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
+
  >scf4_26_27_255_F
-
>scaffold00004_20_21_521_R
+
-
TGAAGATCACCCAGGATCTCAAGGAGTACG
+
-
>scaffold00004_21_22_386_F
+
-
GAAGACTTCGCCACCGAATACCTCAGC
+
-
>scaffold00004_21_22_182_R
+
-
TTCGACGTACACACCACCCTCGCCGTAATC
+
-
>scaffold00004_22_23_594_F
+
-
AATTCAGCGGACAGCGGTACGAGGGTGTAG
+
-
>scaffold00004_22_23_1225_R
+
-
AACAGGACCTGCTCGACAACAAGATCAAG
+
-
>scaffold00004_23_24_720_F
+
-
TCCACCGGAACATGGGTGATACTCGAC
+
-
>scaffold00004_23_24_552_R
+
-
GTTGCCTTTGCACAGGTAGATCCCG
+
-
>scaffold00004_24_25_667_F
+
-
CAAGCCATGTCGAAGCAGCACTACTC
+
-
>scaffold00004_24_25_322_R
+
-
AAGCACCTGCTGTTCACAAGCGAACTGATC
+
-
>scaffold00004_25_26_542_F
+
-
GCAGATCGACGAGTACCTGGAGTTCTAC
+
-
>scaffold00004_25_26_247_R
+
-
AGGAGTCCGACCATGAAGATCAGGAAGATC
+
-
  >scaffold00004_26_27_255_F
+
  GATCGTTCGACTACCCACAATCCGCAGATC
  GATCGTTCGACTACCCACAATCCGCAGATC
-
  >scaffold00004_26_27_671_R
+
  >scf4_26_27_671_R
  GGTAGAGCAGCCATTCCAGTTCCTGTG
  GGTAGAGCAGCCATTCCAGTTCCTGTG
-
>scaffold00004_27_28_577_F
+
 
-
CTGTTCACCGAGGAGGGCGAGTTCTTCTTC
+
  >scf4_27_28_485_R
-
  >scaffold00004_27_28_485_R
+
  GAAAACGGGGTGTTCCAGCTTCATCGCTTC
  GAAAACGGGGTGTTCCAGCTTCATCGCTTC
-
  >scaffold00004_28_29_1409_F
+
  >scf4_27_28_577_F
 +
CTGTTCACCGAGGAGGGCGAGTTCTTCTTC
 +
 
 +
>scf4_28_29_1409_F
  AACCGACACACCTGCAGTTGCCGTACCAAC
  AACCGACACACCTGCAGTTGCCGTACCAAC
-
  >scaffold00004_28_29_207_R
+
  >scf4_28_29_207_R
  TACTCCGGGATAAGCAAGGCTGAGCTG
  TACTCCGGGATAAGCAAGGCTGAGCTG
-
>scaffold00004_29_30_278_F
+
 
-
CATTTGTGACACCTGTCCAGCAGTACG
+
  >scf4_30_31_444_F
-
>scaffold00004_29_30_264_R
+
-
TCTGGTGAGCCGACAGGTATTCCTC
+
-
  >scaffold00004_30_31_444_F
+
  ACCTTCTTGCTCTTACGGCCCACCTTG
  ACCTTCTTGCTCTTACGGCCCACCTTG
-
  >scaffold00004_30_31_739_R
+
  >scf4_30_31_739_R
  GAGTACGTCAAGTTCATTTTCGCCACCAC
  GAGTACGTCAAGTTCATTTTCGCCACCAC
 +
 +
>scf3_1_2_276_F
 +
GACGTGTACATCTCGAACATCTCGCGG
 +
>scf3_1_2_387_R
 +
AAAACGGGCGTTCACCGCATGGTAC
 +
</pre>

Latest revision as of 16:12, 19 February 2011

Contents

Primer Naming Format

Gap primer format

ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R
 
                     CONTIG 1                                                     CONTIG 2
                                ------>
         5'                           3'  
 --------CTCAGGTCAGGCAGGCGTGATGATCTCTTC----------------NNNNNNNNN----------------------------------------
                                                                        GTTAGAGGAGAGTGCGGCTGGGCTCCAATCAT
                                                                        3'                             5'
                                                                        <-------

Positions:                      1105bp                                      529bp

List of primer

primers

 >scaffold00001_2_3_751_F
 CAGAACTGGAGAACGTCCATGCTCGAGAAC
 >scaffold00001_2_3_1360_R
 TCGATCACCAGCTCACGGAAGTCCTCGTTG

 >scaffold00001_3_4_586_F
 GTCAGTGCCGATCAGGATGTTGTCCAC
 >scaffold00001_3_4_183_R
 ATCAACTGCCAGAACTCGTCGGTCCTCATG

 >scaffold00001_4_5_568_F
 TACGTCGAGGAACTGGGCGACTTCAAGACC
 >scaffold00001_4_5_838_R
 CATGTCGTCGATCTGCACGTTGACCAG

 >scaffold00001_9_10_1135_F
 AGACCGGATTGAGTGCTTCCTCTGCTGGTG
 >scaffold00001_9_10_1408_R
 CGACCAATTTCCGACGAGGTGACATTC

 >scaffold00001_11_12_354_F
 AAGGTCAGGATGGCGGTGAACACCTTGGTC
 >scaffold00001_11_12_345_R
 ATGTTGTCGCTGACCTTCGCGATACGGGAG

 >scaffold00001_12_13_1313_F
 TTTCAGGCTTTGAGGATCGAGGTAAACAGG
 >scaffold00001_12_13_315_R
 TCGCATCTTCTCAGCCAGTTCCGAG

 >scaffold00001_15_16_126613_F
 GTTCCCGGTTGCGATCGGTGAGGAT
 >scaffold00001_15_16_569_R
 CTTCTACCACGACACACACACCGATCC

 >scaffold00001_17_18_225_F
 GATCGTGACGAACGTGAAGGTCCAC
 >scaffold00001_17_18_408_R
 CAGATCCGGTACAGCTCCTTGTATTCCAG

 >scaffold00001_18_19_320_F
 CAGATAGATGGTGAAGTCAGGACGACGG
 >scaffold00001_18_19_177_R
 ATGAACTTTCCACCGATGCCGGTCGTCTTC

 >scaffold00001_21_22_243_F
 AAGGACGGACGTGGACATCGATCAGCAATC
 >scaffold00001_21_22_1379_R
 AGGACTCCTGTACGTCGATCACGACGAGTG

 >scaffold00001_23_24_410_F
 ACTGACCACGGTGCAGCTCTACTTCAC
 >scaffold00001_23_24_1184_R
 AGCGTGTAGTCGGCGGTATACACCTTC

 >scaffold00001_25_26_203_F
 GTCGAGGGGATCAACACAAGTGGAATCAAG
 >scaffold00001_25_26_184_R
 GTACCGTCGACAAGCAACTCCTGATCGTC

 >scaffold00001_27_28_280_F
 TGACGTGAGGGGACTACGGATGTAC
 >scaffold00001_27_28_260_R
 ACTTCGACAGGTAGGCCTTGCAGAG

 >scaffold00001_31_32_566_F
 AAACGAGCCGTACAACGGGCAACTG
 >scaffold00001_31_32_169_R
 ATCTTCCTGACCTCGTCGGTGTGGTACTTC

 >scaffold00001_34_35_1071_F
 CAGTACGTCATCTTCGACCGGACCATTTG
 >scaffold00001_34_35_2096_R
 AGTCGTCGTCGTCGAACATCGTGAC

 >scaffold00001_35_36_685_F
 GTCGTTCAGGTTCAGGTAGAACTCGGTGAC
 >scaffold00001_35_36_0_R
 CCCGACCGAACGGCACGCACACAA

 >scaffold00001_36_37_1073_F
 GATCGGTTTGCCGCGGTCGTTGTATTCGAC
 >scaffold00001_36_37_740_R
 ATACGGCTTCGTCGAGAACCTGACC

 >scaffold00001_38_39_612_F
 AACATGTCGTTGAGCGCGGCCAGTTTG
 >scaffold00001_38_39_672_R
 CTCTTCGACGAGATCATCCAGGTGCTG

 >scaffold00001_41_42_205_F
 ACCGAAGCAGTCGAAGACGGTACAC
 >scaffold00001_41_42_339_R
 GTCTTCTTCTGGCCCCGCAACGAGATCATG

 >scaffold00001_42_43_389_F
 TAAAATCTGTGATCTGGGCTAACCTTGGGG
 >scaffold00001_42_43_223_R
 GTGATCGGTGTGTTCGTGGCTGACCAGTAC

 >scaffold00001_45_46_401_F
 GGATCAGCTCCGGATAGTTGAAGTCGTC
 >scaffold00001_45_46_423_R
 ACAACGAGGACTTCGACAACGGCTTCAAGG

 >scaffold00001_47_48_1970_F
 GACGACTACCTGCGTAAACCCTTCGACCTG
 >scaffold00001_47_48_1044_R
 CACCAATCTGCTTGTGGGTCTGGTGCTGAC

 >scaffold00001_48_49_1639_F
 GATGTTCATCGACCTGGAGAACGTGC
 >scaffold00001_48_49_244_R
 CATCTCTCCCGTCAGCTCATCGTTCGAAG

 >scaffold00001_49_50_1297_F
 CAGGGTTTCCTGAGCCACCTTGAAC
 >scaffold00001_49_50_822_R
 CTGTTCTCCCAGGACCCGATCTTCAACTC

 >scaffold00001_50_51_227_F
 CGTGCCATGTTCTGAACGAGAACGATC
 >scaffold00001_50_51_860_R
 GTGACCAGCGAGTGAATCTTGTCGCGTTG

 >scaffold00001_55_56_239_F
 CACCTGACCTCTGACAATGAACGCCGAAAG
 >scaffold00001_55_56_206_R
 GATTGGCTTCTATACAAACTAAGGTGGCGC

 >scaffold00001_57_58_1204_F
 TCATGCTGTCCGTATCCATTTAGGACCTTC
 >scaffold00001_57_58_939_R
 TTCTACCGTGCCTACTTCGAGACCGAC

 >scaffold00001_61_62_285_F
 TCAGGTTGAGATCGACCATCGCGAG
 >scaffold00001_61_62_944_R
 TTGCACCATCAGCTCAGCATCGTCTTCGAC

 >scaffold00001_62_63_898_F
 CTGATGATCAAGGTGTTGCGGCTGTC
 >scaffold00001_62_63_367_R
 GTTGTAGCCGCCGAAGTCGTTCATGCTGAC

 >scaffold00001_67_68_5122_F
 GACCGTTGCGACCAGATATTCGGCGATT
 >scaffold00001_67_68_0_R
 AACCGCTGACCGCGTTCGTGCTGTTCT

 >scaffold00001_71_72_349_F
 AACCCACGCATCCCACTCAACGAGCTGAAC
 >scaffold00001_71_72_0_R
 TCGTTGTCCACCACGCCGACGAACA

 >scaffold00001_73_74_2968_F
 TACGCGGCAGTCGGACGGGTGTGTT
 >scaffold00001_73_74_0_R
 GCTCACCGTGCCGGTGAGGACCAA

 >scaffold00001_75_76_788_F
 GTCGACGTTTCATCACGTCACTGGGTG
 >scaffold00001_75_76_0_R
 GTGCGCCAAGACCTGCGCGAACAGT

 >scaffold00001_76_77_1854_F
 GTGATCAAGGTGATCGAAGCCCTGC
 >scaffold00001_76_77_535_R
 ATCTACAACCACCTGGGCGGCAAGGAGAAC

 >scaffold00001_80_81_426_F
 CACTGCCCAAACCGATCTATCCGACGTTC
 >scaffold00001_80_81_202_R
 GATCCAAGCTGTCTTAACGAGGAGTTGCC

 >scaffold00001_87_88_204_F
 GAAGTGATCACGCGATCCACGGGATTCCAC
 >scaffold00001_87_88_267_R
 AGCTTCCAGACCCTGGTCAACAGTC

 >scaffold00001_88_89_1973_F
 AGTAGCCGATGACCCCGTTGAGTTG
 >scaffold00001_88_89_463_R
 CAGGTGTTCAACCTCGCCCAGACCTTCTAC

 >scaffold00003_3_4_218_F
 ACAAGCTCATTGCCTACTAGATCGACTTCG
 >scaffold00003_3_4_1054_R
 GCATCGTCCAACGGTAGCCTTCTTTC

 >scaffold00003_7_8_365_F
 ATCCGACGAGTACGACATCCACCTG
 >scaffold00003_7_8_601_R
 CAGACCAACTACGCCACAAGCAAGG

 >scaffold00003_8_9_472_F
 CTGTTCCAGACCTTCCTCACCGTGCTTGAC
 >scaffold00003_8_9_218_R
 CTTATGCCCTGACAGAAGGTGCAAGATCAG

 >scaffold00003_10_11_1436_F
 TGGTCCTGCCGTACTTCCTGATCACCACTG
 >scaffold00003_10_11_232_R
 GATGGGACAGACTTCGTACACCGGTTG

 >scaffold00003_11_12_266_F
 CTGTATTGATGATCCACTCGCTGCCGCTC
 >scaffold00003_11_12_1184_R
 TACAGTGATCGGCCGTTGTTGGAGTGTGTG

 >scaffold00003_17_18_314_F
 TCTGAACGAGACGGAATGGGAGATCCTCAC
 >scaffold00003_17_18_222_R
 GACATCACCATCCAGGCAGAGAACATGCTG

 >scaffold00003_19_20_655_F
 GAAGGAACGGCAACTTTCGGTCACGGTAC
 >scaffold00003_19_20_485_R
 CAAGGAGACCGTCTACAAGATCTGGCATC

 >scaffold00003_23_24_235_F
 CCCAGGGAATATAACGATGGTTCAGGTCAG
 >scaffold00003_23_24_1163_R
 GAACTGCTGGTTCATCGAATGGTCGTTGAC

 >scaffold00003_24_25_268_F
 CTCGAAGTCCCGGTCAAGAAGATCCTCAC
 >scaffold00003_24_25_666_R
 TATCGTTCGCGTCGATGGCCGACAACATTG

 >scaffold00003_26_27_1270_F
 GAAATCAAGATCCTGAACCAGTCCCGGAAG
 >scaffold00003_26_27_244_R
 CCCGATTCCTTGTCCTGGAACAGATAGAAG

 >scaffold00003_27_28_833_F
 GTACTCGCCGACTTTCCCGTTCTCATC
 >scaffold00003_27_28_474_R
 GTACACCTCGTGCTCGGGACACAAATGGTC

 >scaffold00003_28_29_210_F
 GTACACGTGCCGTTCCCGTTTCTGGTGAAC
 >scaffold00003_28_29_173_R
 ACGGCGTCACGAACAACACCTACTG

 >scaffold00003_30_31_256_F
 TTCCGGGGATGAGTAGCCGACCAATTGTCC
 >scaffold00003_30_31_1239_R
 GATCACCGGTTGGTAGACCAGATCCAG

 >scaffold00003_31_32_351_F
 CACAAATTGAACTTGAGCTGGGATGTCAGG
 >scaffold00003_31_32_565_R
 CAGGATGACAGTGTGAGTCAGCCATGGAC

 >scaffold00003_35_36_707_F
 AAGTTGAAGGAAATGGGCGAGTTCGTGAAG
 >scaffold00003_35_36_243_R
 CAGGTTGAACATCTCCTGGACCAGCGAAC

 >scaffold00003_42_43_317_F
 GTTCCTGTCCTCGTGCAGCACAATTCGATC
 >scaffold00003_42_43_298_R
 ACCGGGGATCATCGTGGTCTTGTAC

 >scaffold00003_43_44_737_F
 ATCGTTCGCAGCGGCATCGATTCAC
 >scaffold00003_43_44_992_R
 TGAAGCTGACCAAGCTGGTGGTCTC

 >scaffold00003_45_46_422_F
 GTGATGCCCTCGGATCCCAGTTCTTCGTAC
 >scaffold00003_45_46_399_R
 AACTCGGTCAAGCTCGAGTTCCTGGTG

 >scaffold00003_47_48_427_F
 CATCACGGCGAACAACAACCAGACCTAC
 >scaffold00003_47_48_271_R
 GAACAACGGCATGAGGTCCTTGAACATGAC

 >scaffold00003_48_49_532_F
 GAAGGGTCAACAGTGAGATCGCCTGGTTC
 >scaffold00003_48_49_741_R
 TGTAGCTGCCCTTCTCCTCCTGTTC

 >scaffold00003_49_50_208_F
 GTGGTGTGCATCGTGCAGATCCCGTTCTAC
 >scaffold00003_49_50_975_R
 AACCCGGAGCCGAAGAGTTCGAGAAAC

 >scaffold00003_50_51_1445_F
 TGGCGTACCTGGAGAAGGTGGTTTC
 >scaffold00003_50_51_439_R
 CAATGGCACCCTCACATATGGCAGATAATTG

 >scaffold00004_8_9_447_F
 GAACCTGCTGTTCATGGTCGGTCTG
 >scaffold00004_8_9_234_R
 ACAGTGGTCTCCGTCTACAGTCCAC

 >scaffold00004_12_13_750_F
 TCGATGGACATGCTCTCCTCCGAGATCGTC
 >scaffold00004_12_13_895_R
 TATGCGAGCGTCCGAAAAAGATCGACCAGC

 >scaffold00004_13_14_550_F
 GAGCACATCAAGATCACTGACAATGGGATC
 >scaffold00004_13_14_243_R
 AACACTCCGAAATCCGCTGGCTCTC

 >scaffold00004_14_15_556_F
 CTGGTGGGACTCGTTGAGCATCCAATG
 >scaffold00004_14_15_220_R
 CCGAGGGAAAGAACCGGACATTGTG

 >scaffold00004_15_16_1515_F
 CTTCACCGGCCTGTTCTTCGTCATCAC
 >scaffold00004_15_16_167_R
 CAGTACCTGTGGCGTCACACGCGATAAGAG

 >scaffold00004_16_17_236_F
 GGTTCGACACCAGGATTTCGAGGTCAC
 >scaffold00004_16_17_467_R
 GATCTGGTTCCACATCTGTCCCTGGTG

 >scaffold00004_18_19_226_F
 GTGTGATCGACACGGTTACGTCGAAG
 >scaffold00004_18_19_492_R
 TTAACAGGGGGCCCTTCCTTCAACACGGTC

 >scaffold00004_20_21_2075_F
 AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG
 >scaffold00004_20_21_521_R
 TGAAGATCACCCAGGATCTCAAGGAGTACG

 >scaffold00004_21_22_386_F
 GAAGACTTCGCCACCGAATACCTCAGC
 >scaffold00004_21_22_182_R
 TTCGACGTACACACCACCCTCGCCGTAATC

 >scaffold00004_23_24_720_F
 TCCACCGGAACATGGGTGATACTCGAC
 >scaffold00004_23_24_552_R
 GTTGCCTTTGCACAGGTAGATCCCG

 >scaffold00004_24_25_667_F
 CAAGCCATGTCGAAGCAGCACTACTC
 >scaffold00004_24_25_322_R
 AAGCACCTGCTGTTCACAAGCGAACTGATC

 >scaffold00004_25_26_542_F
 GCAGATCGACGAGTACCTGGAGTTCTAC
 >scaffold00004_25_26_247_R
 AGGAGTCCGACCATGAAGATCAGGAAGATC

 >scaffold00004_29_30_278_F
 CATTTGTGACACCTGTCCAGCAGTACG
 >scaffold00004_29_30_264_R
 TCTGGTGAGCCGACAGGTATTCCTC

 >scf1_10_11_191_R
 ATTCCTCGCGCAGTTGACCCAGTTC
 >scf1_10_11_533_F
 TTCGCCACCACCGGTCTAGGATGAGAGATC
 
 >scf1_13_14_1221_R
 AGTCGTCGAAGCGCAGAAGGTACATGTAGG
 >scf1_13_14_961_F
 GTCATCGGACAGCCGATTCTTCGACGACTG

 >scf1_14_15_1407_F
 CTTGTTTCGATAGCATTACCGCCCAGTAAC
 >scf1_14_15_533_R
 GTACTCGTCGAAGGTGGACACGTTCACTC

 >scf1_16_17_1785_F
 CTCGAAGACGTCAACAACCACACCAATCTG
 >scf1_16_17_478_R
 CTCGTAACTCTCCATCGGTTCTCCAGTAAC

 >scf1_22_23_238_F
 GTTTCACTCATCGGTGTACCCTCCCGATC
 >scf1_22_23_244_R
 GAAGTTCGTCATCGAGAACGTCTCCGTGC

 >scf1_24_25_444_F
 ATTCGACGGGCAGGACGTCAACAAG
 >scf1_24_25_458_R
 TAGGTGTGCTCCAGGTAGGTGAGGAAC

 >scf1_26_27_369_F
 ATGAGCGAGCAGGTCTTCCTCCTAC
 >scf1_26_27_706_R
 GAACACGCCGTAGTTGTTCAGCGTGAAG

 >scf1_29_30_1643_F
 CCGATCCAGGCGAAGATCAGATTGGTC
 >scf1_29_30_767_R
 ATCGACATAGCGCTCCAACAGATCGGTGAC

 >scf1_32_33_169_R
 TTGTCAATGTAGCGTTGACGGGTGCCTGAC
 >scf1_32_33_266_F
 AACATTGACAGGGTGGCACGCAGTTCG

 >scf1_33_34_365_R
 GTTCCCTGCTGCTGAACTGGTTCTTCAC
 >scf1_33_34_795_F
 GTCGACGACGAGAATCCGGTTCATG

 >scf1_37_38_498_F
 TTCGACGAACTGTGGCAACGCTTCTCC
 >scf1_37_38_562_R
 AGGACGTCATGCACGGTCAACACAC

 >scf1_40_41_522_F
 GACTACAAGCCGGAGAACGTGCTCATCTC
 >scf1_40_41_660_R
 GTGCAGAAGGGATAGAGGGTGACGTAC

 >scf1_43_44_440_F
 CTCGGAGGAAAACGGTGAACAGTCAGCAAC
 >scf1_43_44_563_R
 TGAACGCCTCCACCCAGCATTTGATCACAG

 >scf1_44_45_485_R
 GTGTTGCGATCGGATTCGTAGCTGAC
 >scf1_44_45_93801_F
 ACACTGCTGCCGCCACGGTTGACTGT

 >scf1_46_47_370_F
 GTAAACTCCTGATCTCAGCCGCTCTCGTG
 >scf1_46_47_845_R
 CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG

 >scf1_53_54_414_F
 TTCTGGAAGACCATCTGCAGACCGATCTCG
 >scf1_53_54_823_R
 TACGCGAAGGGACGTTAGAGATGGCAC

 >scf1_54_55_1250_F
 CTGGAACCGGATGATGTTGGAGTCCTC
 >scf1_54_55_175_R
 TACCGAGGAACTGATCGACCTCTCC

 >scf1_56_57_184_R
 CAAACCGGTGGTCGGGCTGATCATGTATTC
 >scf1_56_57_412_F
 GTACTCACGATGGTCCTTCTCCTCGTTG

 >scf1_59_60_1979_F
 GTGACCAGCCAGGTCTGTTCGAATCTCAC
 >scf1_59_60_625_R
 GAGATCATGTACGCCGACTTCAGCTTCGTG

 >scf1_63_64_1286_R
 CGATAGGTGAGACTCGATGCATGGTTAGTG
 >scf1_63_64_711_F
 ACGTAGTTCCCGACCGTAGAGCAGTTC

 >scf1_66_67_346_R
 ACTGTTCCTGCTGTACTCCTCGGTG
 >scf1_66_67_428_F
 CTTCGTCACTGGCTGTGTCAAGGTCAAC

 >scf1_6_7_870_R
 CGATCTCCACGTTTGACAGGATGCTCTG
 >scf1_6_7_939_F
 ATCAGCGGTTACGTCTGGTGGTGAC

 >scf1_7_8_545_R
 ATCAGTTTGATCGGCGAGACGTGCGAGACC
 >scf1_7_8_818_F
 GAACTGGAGTTGATGGAGCAGCGCGAAAC

 >scf3_12_13_2070_F
 CTCCAGCAGGGTTTCATCGATTCGGTAG
 >scf3_12_13_324_R
 CTGGACCAGTTCACCCTCCAGATCATG

 >scf3_18_19_471_R
 GTATACCGCAACATCGGCGAAATGAAGTC
 >scf3_18_19_746_F
 GTTGCTTCGAATCTGGTGACGGGAG

 >scf3_20_21_200_R
 GAAGATCGGCAGCTCCATCGTCTCATC
 >scf3_20_21_294_F
 ATCAGCCCCGAGCAATTGACCGACCTGAAC

 >scf3_21_22_1034_F
 GAAGTAACCGGTGGTGTTTTCGGCCAG
 >scf3_21_22_354_R
 ATCTCGCCGGTTACAAGCAGATCCTC

 >scf3_22_23_1299_F
 TTCAGATGGTTAAGGACAGCTTCATGAGCG
 >scf3_22_23_368_R
 ACGTGATCGGTGGGAACTTCGACTC

 >scf3_25_26_360_F
 GAAAGGCTGACCATCTACCCGGAGTAC
 >scf3_25_26_636_R
 TGATCCACGTGTCCGTACATCATCGTG

 >scf3_33_34_1969_F
 GTCCTCAAGCATCGCAAGCGCAAGGAAAAG
 >scf3_33_34_485_R
 CTCCAACTGCTCGGAGAGGAACTTGTAAC

 >scf3_34_35_222_R
 ATCTCCGCAGTCATATCGGTACGGG
 >scf3_34_35_491_F
 GAACGAGACCAGGAGACATAGTGGCGAGTC

 >scf3_36_37_355_F
 CTGCTCTTCCTCGGCATGCTCATGTTC
 >scf3_36_37_430_R
 ATAGCCCGCCCACTGCATGACAAAC

 >scf3_38_39_613_R
 GTACGAGCAGTTCACCAGTTGCGTG
 >scf3_38_39_791_F
 AACTGTCGACGAAGAACCGCGTCATGAGAG

 >scf3_39_40_333_R
 CTGATCCGTCATGGCGCACATTCCACGATG
 >scf3_39_40_351_F
 CACTTGTCTTCTCCTCCGGATACCTGG

 >scf3_44_45_1117_F
 CTCTGTGAGTACGTGGAACAGGCGATG
 >scf3_44_45_224_R
 AAGATCACCACGGCGTCGACACAGCTCAAC

 >scf3_6_7_245_R
 GCATGCTGTTGTTGATGCTGTCCTTCAG
 >scf3_6_7_918_F
 ATGCTCAGTCCGGGAGTACAGCTCGGTAAC

 >scf3_9_10_212_R
 TTTCCGTTGAGCCGCCACTCGTACTTGTAG
 >scf3_9_10_376_F
 CATGCACAAGCAGGACCTCATCGAG

 >scf4_19_20_273_F
 GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC
 >scf4_19_20_878_R
 GACGAGCAACTGGACTCGCTGAACAAG

 >scf4_26_27_255_F
 GATCGTTCGACTACCCACAATCCGCAGATC
 >scf4_26_27_671_R
 GGTAGAGCAGCCATTCCAGTTCCTGTG

 >scf4_27_28_485_R
 GAAAACGGGGTGTTCCAGCTTCATCGCTTC
 >scf4_27_28_577_F
 CTGTTCACCGAGGAGGGCGAGTTCTTCTTC

 >scf4_28_29_1409_F
 AACCGACACACCTGCAGTTGCCGTACCAAC
 >scf4_28_29_207_R
 TACTCCGGGATAAGCAAGGCTGAGCTG

 >scf4_30_31_444_F
 ACCTTCTTGCTCTTACGGCCCACCTTG
 >scf4_30_31_739_R
 GAGTACGTCAAGTTCATTTTCGCCACCAC

 >scf3_1_2_276_F
 GACGTGTACATCTCGAACATCTCGCGG
 >scf3_1_2_387_R
 AAAACGGGCGTTCACCGCATGGTAC
Retrieved from "http://compbio.korea.ac.kr/wiki/index.php/Primer_Verrucosispora_gap"
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