Primer Verrucosispora gap
From CSBLwiki
(Difference between revisions)
(→primer intergenic) |
|||
| (9 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
==Primer Naming Format== | ==Primer Naming Format== | ||
| - | ===Scf&SolCtg primer format=== | + | <!-- ===Scf&SolCtg primer format=== --> |
===Gap primer format=== | ===Gap primer format=== | ||
*>[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse] | *>[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse] | ||
| - | + | <pre> | |
| + | ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R | ||
CONTIG 1 CONTIG 2 | CONTIG 1 CONTIG 2 | ||
| Line 13: | Line 14: | ||
<------- | <------- | ||
| - | + | Positions: 1105bp 529bp | |
| + | </pre> | ||
==List of primer== | ==List of primer== | ||
| + | <!-- | ||
===primers of scaffold & sole contigs=== | ===primers of scaffold & sole contigs=== | ||
====scf1==== | ====scf1==== | ||
| Line 52: | Line 55: | ||
TGGCGATTGGGACGAAGTCGTAACAAGGTA | TGGCGATTGGGACGAAGTCGTAACAAGGTA | ||
| + | --> | ||
*By Using Primer3 | *By Using Primer3 | ||
*"Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer). | *"Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer). | ||
| - | |||
| - | |||
| + | ===primers=== | ||
| + | *115 gaps (230 primers) | ||
| + | |||
| + | <pre> | ||
>scaffold00001_2_3_751_F | >scaffold00001_2_3_751_F | ||
CAGAACTGGAGAACGTCCATGCTCGAGAAC | CAGAACTGGAGAACGTCCATGCTCGAGAAC | ||
>scaffold00001_2_3_1360_R | >scaffold00001_2_3_1360_R | ||
TCGATCACCAGCTCACGGAAGTCCTCGTTG | TCGATCACCAGCTCACGGAAGTCCTCGTTG | ||
| + | |||
>scaffold00001_3_4_586_F | >scaffold00001_3_4_586_F | ||
GTCAGTGCCGATCAGGATGTTGTCCAC | GTCAGTGCCGATCAGGATGTTGTCCAC | ||
>scaffold00001_3_4_183_R | >scaffold00001_3_4_183_R | ||
ATCAACTGCCAGAACTCGTCGGTCCTCATG | ATCAACTGCCAGAACTCGTCGGTCCTCATG | ||
| + | |||
>scaffold00001_4_5_568_F | >scaffold00001_4_5_568_F | ||
TACGTCGAGGAACTGGGCGACTTCAAGACC | TACGTCGAGGAACTGGGCGACTTCAAGACC | ||
>scaffold00001_4_5_838_R | >scaffold00001_4_5_838_R | ||
CATGTCGTCGATCTGCACGTTGACCAG | CATGTCGTCGATCTGCACGTTGACCAG | ||
| + | |||
>scaffold00001_9_10_1135_F | >scaffold00001_9_10_1135_F | ||
AGACCGGATTGAGTGCTTCCTCTGCTGGTG | AGACCGGATTGAGTGCTTCCTCTGCTGGTG | ||
>scaffold00001_9_10_1408_R | >scaffold00001_9_10_1408_R | ||
CGACCAATTTCCGACGAGGTGACATTC | CGACCAATTTCCGACGAGGTGACATTC | ||
| + | |||
>scaffold00001_11_12_354_F | >scaffold00001_11_12_354_F | ||
AAGGTCAGGATGGCGGTGAACACCTTGGTC | AAGGTCAGGATGGCGGTGAACACCTTGGTC | ||
>scaffold00001_11_12_345_R | >scaffold00001_11_12_345_R | ||
ATGTTGTCGCTGACCTTCGCGATACGGGAG | ATGTTGTCGCTGACCTTCGCGATACGGGAG | ||
| + | |||
>scaffold00001_12_13_1313_F | >scaffold00001_12_13_1313_F | ||
TTTCAGGCTTTGAGGATCGAGGTAAACAGG | TTTCAGGCTTTGAGGATCGAGGTAAACAGG | ||
>scaffold00001_12_13_315_R | >scaffold00001_12_13_315_R | ||
TCGCATCTTCTCAGCCAGTTCCGAG | TCGCATCTTCTCAGCCAGTTCCGAG | ||
| + | |||
>scaffold00001_15_16_126613_F | >scaffold00001_15_16_126613_F | ||
GTTCCCGGTTGCGATCGGTGAGGAT | GTTCCCGGTTGCGATCGGTGAGGAT | ||
>scaffold00001_15_16_569_R | >scaffold00001_15_16_569_R | ||
CTTCTACCACGACACACACACCGATCC | CTTCTACCACGACACACACACCGATCC | ||
| + | |||
>scaffold00001_17_18_225_F | >scaffold00001_17_18_225_F | ||
GATCGTGACGAACGTGAAGGTCCAC | GATCGTGACGAACGTGAAGGTCCAC | ||
>scaffold00001_17_18_408_R | >scaffold00001_17_18_408_R | ||
CAGATCCGGTACAGCTCCTTGTATTCCAG | CAGATCCGGTACAGCTCCTTGTATTCCAG | ||
| + | |||
>scaffold00001_18_19_320_F | >scaffold00001_18_19_320_F | ||
CAGATAGATGGTGAAGTCAGGACGACGG | CAGATAGATGGTGAAGTCAGGACGACGG | ||
>scaffold00001_18_19_177_R | >scaffold00001_18_19_177_R | ||
ATGAACTTTCCACCGATGCCGGTCGTCTTC | ATGAACTTTCCACCGATGCCGGTCGTCTTC | ||
| + | |||
>scaffold00001_21_22_243_F | >scaffold00001_21_22_243_F | ||
AAGGACGGACGTGGACATCGATCAGCAATC | AAGGACGGACGTGGACATCGATCAGCAATC | ||
>scaffold00001_21_22_1379_R | >scaffold00001_21_22_1379_R | ||
AGGACTCCTGTACGTCGATCACGACGAGTG | AGGACTCCTGTACGTCGATCACGACGAGTG | ||
| + | |||
>scaffold00001_23_24_410_F | >scaffold00001_23_24_410_F | ||
ACTGACCACGGTGCAGCTCTACTTCAC | ACTGACCACGGTGCAGCTCTACTTCAC | ||
>scaffold00001_23_24_1184_R | >scaffold00001_23_24_1184_R | ||
AGCGTGTAGTCGGCGGTATACACCTTC | AGCGTGTAGTCGGCGGTATACACCTTC | ||
| + | |||
>scaffold00001_25_26_203_F | >scaffold00001_25_26_203_F | ||
GTCGAGGGGATCAACACAAGTGGAATCAAG | GTCGAGGGGATCAACACAAGTGGAATCAAG | ||
>scaffold00001_25_26_184_R | >scaffold00001_25_26_184_R | ||
GTACCGTCGACAAGCAACTCCTGATCGTC | GTACCGTCGACAAGCAACTCCTGATCGTC | ||
| + | |||
>scaffold00001_27_28_280_F | >scaffold00001_27_28_280_F | ||
TGACGTGAGGGGACTACGGATGTAC | TGACGTGAGGGGACTACGGATGTAC | ||
>scaffold00001_27_28_260_R | >scaffold00001_27_28_260_R | ||
ACTTCGACAGGTAGGCCTTGCAGAG | ACTTCGACAGGTAGGCCTTGCAGAG | ||
| + | |||
>scaffold00001_31_32_566_F | >scaffold00001_31_32_566_F | ||
AAACGAGCCGTACAACGGGCAACTG | AAACGAGCCGTACAACGGGCAACTG | ||
>scaffold00001_31_32_169_R | >scaffold00001_31_32_169_R | ||
ATCTTCCTGACCTCGTCGGTGTGGTACTTC | ATCTTCCTGACCTCGTCGGTGTGGTACTTC | ||
| + | |||
>scaffold00001_34_35_1071_F | >scaffold00001_34_35_1071_F | ||
CAGTACGTCATCTTCGACCGGACCATTTG | CAGTACGTCATCTTCGACCGGACCATTTG | ||
>scaffold00001_34_35_2096_R | >scaffold00001_34_35_2096_R | ||
AGTCGTCGTCGTCGAACATCGTGAC | AGTCGTCGTCGTCGAACATCGTGAC | ||
| + | |||
>scaffold00001_35_36_685_F | >scaffold00001_35_36_685_F | ||
GTCGTTCAGGTTCAGGTAGAACTCGGTGAC | GTCGTTCAGGTTCAGGTAGAACTCGGTGAC | ||
>scaffold00001_35_36_0_R | >scaffold00001_35_36_0_R | ||
CCCGACCGAACGGCACGCACACAA | CCCGACCGAACGGCACGCACACAA | ||
| + | |||
>scaffold00001_36_37_1073_F | >scaffold00001_36_37_1073_F | ||
GATCGGTTTGCCGCGGTCGTTGTATTCGAC | GATCGGTTTGCCGCGGTCGTTGTATTCGAC | ||
>scaffold00001_36_37_740_R | >scaffold00001_36_37_740_R | ||
ATACGGCTTCGTCGAGAACCTGACC | ATACGGCTTCGTCGAGAACCTGACC | ||
| + | |||
>scaffold00001_38_39_612_F | >scaffold00001_38_39_612_F | ||
AACATGTCGTTGAGCGCGGCCAGTTTG | AACATGTCGTTGAGCGCGGCCAGTTTG | ||
>scaffold00001_38_39_672_R | >scaffold00001_38_39_672_R | ||
CTCTTCGACGAGATCATCCAGGTGCTG | CTCTTCGACGAGATCATCCAGGTGCTG | ||
| + | |||
>scaffold00001_41_42_205_F | >scaffold00001_41_42_205_F | ||
ACCGAAGCAGTCGAAGACGGTACAC | ACCGAAGCAGTCGAAGACGGTACAC | ||
>scaffold00001_41_42_339_R | >scaffold00001_41_42_339_R | ||
GTCTTCTTCTGGCCCCGCAACGAGATCATG | GTCTTCTTCTGGCCCCGCAACGAGATCATG | ||
| + | |||
>scaffold00001_42_43_389_F | >scaffold00001_42_43_389_F | ||
TAAAATCTGTGATCTGGGCTAACCTTGGGG | TAAAATCTGTGATCTGGGCTAACCTTGGGG | ||
>scaffold00001_42_43_223_R | >scaffold00001_42_43_223_R | ||
GTGATCGGTGTGTTCGTGGCTGACCAGTAC | GTGATCGGTGTGTTCGTGGCTGACCAGTAC | ||
| + | |||
>scaffold00001_45_46_401_F | >scaffold00001_45_46_401_F | ||
GGATCAGCTCCGGATAGTTGAAGTCGTC | GGATCAGCTCCGGATAGTTGAAGTCGTC | ||
>scaffold00001_45_46_423_R | >scaffold00001_45_46_423_R | ||
ACAACGAGGACTTCGACAACGGCTTCAAGG | ACAACGAGGACTTCGACAACGGCTTCAAGG | ||
| + | |||
>scaffold00001_47_48_1970_F | >scaffold00001_47_48_1970_F | ||
GACGACTACCTGCGTAAACCCTTCGACCTG | GACGACTACCTGCGTAAACCCTTCGACCTG | ||
>scaffold00001_47_48_1044_R | >scaffold00001_47_48_1044_R | ||
CACCAATCTGCTTGTGGGTCTGGTGCTGAC | CACCAATCTGCTTGTGGGTCTGGTGCTGAC | ||
| + | |||
>scaffold00001_48_49_1639_F | >scaffold00001_48_49_1639_F | ||
GATGTTCATCGACCTGGAGAACGTGC | GATGTTCATCGACCTGGAGAACGTGC | ||
>scaffold00001_48_49_244_R | >scaffold00001_48_49_244_R | ||
CATCTCTCCCGTCAGCTCATCGTTCGAAG | CATCTCTCCCGTCAGCTCATCGTTCGAAG | ||
| + | |||
>scaffold00001_49_50_1297_F | >scaffold00001_49_50_1297_F | ||
CAGGGTTTCCTGAGCCACCTTGAAC | CAGGGTTTCCTGAGCCACCTTGAAC | ||
>scaffold00001_49_50_822_R | >scaffold00001_49_50_822_R | ||
CTGTTCTCCCAGGACCCGATCTTCAACTC | CTGTTCTCCCAGGACCCGATCTTCAACTC | ||
| + | |||
>scaffold00001_50_51_227_F | >scaffold00001_50_51_227_F | ||
CGTGCCATGTTCTGAACGAGAACGATC | CGTGCCATGTTCTGAACGAGAACGATC | ||
>scaffold00001_50_51_860_R | >scaffold00001_50_51_860_R | ||
GTGACCAGCGAGTGAATCTTGTCGCGTTG | GTGACCAGCGAGTGAATCTTGTCGCGTTG | ||
| + | |||
>scaffold00001_55_56_239_F | >scaffold00001_55_56_239_F | ||
CACCTGACCTCTGACAATGAACGCCGAAAG | CACCTGACCTCTGACAATGAACGCCGAAAG | ||
>scaffold00001_55_56_206_R | >scaffold00001_55_56_206_R | ||
GATTGGCTTCTATACAAACTAAGGTGGCGC | GATTGGCTTCTATACAAACTAAGGTGGCGC | ||
| + | |||
>scaffold00001_57_58_1204_F | >scaffold00001_57_58_1204_F | ||
TCATGCTGTCCGTATCCATTTAGGACCTTC | TCATGCTGTCCGTATCCATTTAGGACCTTC | ||
>scaffold00001_57_58_939_R | >scaffold00001_57_58_939_R | ||
TTCTACCGTGCCTACTTCGAGACCGAC | TTCTACCGTGCCTACTTCGAGACCGAC | ||
| + | |||
>scaffold00001_61_62_285_F | >scaffold00001_61_62_285_F | ||
TCAGGTTGAGATCGACCATCGCGAG | TCAGGTTGAGATCGACCATCGCGAG | ||
>scaffold00001_61_62_944_R | >scaffold00001_61_62_944_R | ||
TTGCACCATCAGCTCAGCATCGTCTTCGAC | TTGCACCATCAGCTCAGCATCGTCTTCGAC | ||
| + | |||
>scaffold00001_62_63_898_F | >scaffold00001_62_63_898_F | ||
CTGATGATCAAGGTGTTGCGGCTGTC | CTGATGATCAAGGTGTTGCGGCTGTC | ||
>scaffold00001_62_63_367_R | >scaffold00001_62_63_367_R | ||
GTTGTAGCCGCCGAAGTCGTTCATGCTGAC | GTTGTAGCCGCCGAAGTCGTTCATGCTGAC | ||
| + | |||
>scaffold00001_67_68_5122_F | >scaffold00001_67_68_5122_F | ||
GACCGTTGCGACCAGATATTCGGCGATT | GACCGTTGCGACCAGATATTCGGCGATT | ||
>scaffold00001_67_68_0_R | >scaffold00001_67_68_0_R | ||
AACCGCTGACCGCGTTCGTGCTGTTCT | AACCGCTGACCGCGTTCGTGCTGTTCT | ||
| + | |||
>scaffold00001_71_72_349_F | >scaffold00001_71_72_349_F | ||
AACCCACGCATCCCACTCAACGAGCTGAAC | AACCCACGCATCCCACTCAACGAGCTGAAC | ||
>scaffold00001_71_72_0_R | >scaffold00001_71_72_0_R | ||
TCGTTGTCCACCACGCCGACGAACA | TCGTTGTCCACCACGCCGACGAACA | ||
| + | |||
>scaffold00001_73_74_2968_F | >scaffold00001_73_74_2968_F | ||
TACGCGGCAGTCGGACGGGTGTGTT | TACGCGGCAGTCGGACGGGTGTGTT | ||
>scaffold00001_73_74_0_R | >scaffold00001_73_74_0_R | ||
GCTCACCGTGCCGGTGAGGACCAA | GCTCACCGTGCCGGTGAGGACCAA | ||
| + | |||
>scaffold00001_75_76_788_F | >scaffold00001_75_76_788_F | ||
GTCGACGTTTCATCACGTCACTGGGTG | GTCGACGTTTCATCACGTCACTGGGTG | ||
>scaffold00001_75_76_0_R | >scaffold00001_75_76_0_R | ||
GTGCGCCAAGACCTGCGCGAACAGT | GTGCGCCAAGACCTGCGCGAACAGT | ||
| + | |||
>scaffold00001_76_77_1854_F | >scaffold00001_76_77_1854_F | ||
GTGATCAAGGTGATCGAAGCCCTGC | GTGATCAAGGTGATCGAAGCCCTGC | ||
>scaffold00001_76_77_535_R | >scaffold00001_76_77_535_R | ||
ATCTACAACCACCTGGGCGGCAAGGAGAAC | ATCTACAACCACCTGGGCGGCAAGGAGAAC | ||
| + | |||
>scaffold00001_80_81_426_F | >scaffold00001_80_81_426_F | ||
CACTGCCCAAACCGATCTATCCGACGTTC | CACTGCCCAAACCGATCTATCCGACGTTC | ||
>scaffold00001_80_81_202_R | >scaffold00001_80_81_202_R | ||
GATCCAAGCTGTCTTAACGAGGAGTTGCC | GATCCAAGCTGTCTTAACGAGGAGTTGCC | ||
| + | |||
>scaffold00001_87_88_204_F | >scaffold00001_87_88_204_F | ||
GAAGTGATCACGCGATCCACGGGATTCCAC | GAAGTGATCACGCGATCCACGGGATTCCAC | ||
>scaffold00001_87_88_267_R | >scaffold00001_87_88_267_R | ||
AGCTTCCAGACCCTGGTCAACAGTC | AGCTTCCAGACCCTGGTCAACAGTC | ||
| + | |||
>scaffold00001_88_89_1973_F | >scaffold00001_88_89_1973_F | ||
AGTAGCCGATGACCCCGTTGAGTTG | AGTAGCCGATGACCCCGTTGAGTTG | ||
>scaffold00001_88_89_463_R | >scaffold00001_88_89_463_R | ||
CAGGTGTTCAACCTCGCCCAGACCTTCTAC | CAGGTGTTCAACCTCGCCCAGACCTTCTAC | ||
| + | |||
>scaffold00003_3_4_218_F | >scaffold00003_3_4_218_F | ||
ACAAGCTCATTGCCTACTAGATCGACTTCG | ACAAGCTCATTGCCTACTAGATCGACTTCG | ||
>scaffold00003_3_4_1054_R | >scaffold00003_3_4_1054_R | ||
GCATCGTCCAACGGTAGCCTTCTTTC | GCATCGTCCAACGGTAGCCTTCTTTC | ||
| + | |||
>scaffold00003_7_8_365_F | >scaffold00003_7_8_365_F | ||
ATCCGACGAGTACGACATCCACCTG | ATCCGACGAGTACGACATCCACCTG | ||
>scaffold00003_7_8_601_R | >scaffold00003_7_8_601_R | ||
CAGACCAACTACGCCACAAGCAAGG | CAGACCAACTACGCCACAAGCAAGG | ||
| + | |||
>scaffold00003_8_9_472_F | >scaffold00003_8_9_472_F | ||
CTGTTCCAGACCTTCCTCACCGTGCTTGAC | CTGTTCCAGACCTTCCTCACCGTGCTTGAC | ||
>scaffold00003_8_9_218_R | >scaffold00003_8_9_218_R | ||
CTTATGCCCTGACAGAAGGTGCAAGATCAG | CTTATGCCCTGACAGAAGGTGCAAGATCAG | ||
| + | |||
>scaffold00003_10_11_1436_F | >scaffold00003_10_11_1436_F | ||
TGGTCCTGCCGTACTTCCTGATCACCACTG | TGGTCCTGCCGTACTTCCTGATCACCACTG | ||
>scaffold00003_10_11_232_R | >scaffold00003_10_11_232_R | ||
GATGGGACAGACTTCGTACACCGGTTG | GATGGGACAGACTTCGTACACCGGTTG | ||
| + | |||
>scaffold00003_11_12_266_F | >scaffold00003_11_12_266_F | ||
CTGTATTGATGATCCACTCGCTGCCGCTC | CTGTATTGATGATCCACTCGCTGCCGCTC | ||
>scaffold00003_11_12_1184_R | >scaffold00003_11_12_1184_R | ||
TACAGTGATCGGCCGTTGTTGGAGTGTGTG | TACAGTGATCGGCCGTTGTTGGAGTGTGTG | ||
| + | |||
>scaffold00003_17_18_314_F | >scaffold00003_17_18_314_F | ||
TCTGAACGAGACGGAATGGGAGATCCTCAC | TCTGAACGAGACGGAATGGGAGATCCTCAC | ||
>scaffold00003_17_18_222_R | >scaffold00003_17_18_222_R | ||
GACATCACCATCCAGGCAGAGAACATGCTG | GACATCACCATCCAGGCAGAGAACATGCTG | ||
| + | |||
>scaffold00003_19_20_655_F | >scaffold00003_19_20_655_F | ||
GAAGGAACGGCAACTTTCGGTCACGGTAC | GAAGGAACGGCAACTTTCGGTCACGGTAC | ||
>scaffold00003_19_20_485_R | >scaffold00003_19_20_485_R | ||
CAAGGAGACCGTCTACAAGATCTGGCATC | CAAGGAGACCGTCTACAAGATCTGGCATC | ||
| + | |||
>scaffold00003_23_24_235_F | >scaffold00003_23_24_235_F | ||
CCCAGGGAATATAACGATGGTTCAGGTCAG | CCCAGGGAATATAACGATGGTTCAGGTCAG | ||
>scaffold00003_23_24_1163_R | >scaffold00003_23_24_1163_R | ||
GAACTGCTGGTTCATCGAATGGTCGTTGAC | GAACTGCTGGTTCATCGAATGGTCGTTGAC | ||
| + | |||
>scaffold00003_24_25_268_F | >scaffold00003_24_25_268_F | ||
CTCGAAGTCCCGGTCAAGAAGATCCTCAC | CTCGAAGTCCCGGTCAAGAAGATCCTCAC | ||
>scaffold00003_24_25_666_R | >scaffold00003_24_25_666_R | ||
TATCGTTCGCGTCGATGGCCGACAACATTG | TATCGTTCGCGTCGATGGCCGACAACATTG | ||
| + | |||
>scaffold00003_26_27_1270_F | >scaffold00003_26_27_1270_F | ||
GAAATCAAGATCCTGAACCAGTCCCGGAAG | GAAATCAAGATCCTGAACCAGTCCCGGAAG | ||
>scaffold00003_26_27_244_R | >scaffold00003_26_27_244_R | ||
CCCGATTCCTTGTCCTGGAACAGATAGAAG | CCCGATTCCTTGTCCTGGAACAGATAGAAG | ||
| + | |||
>scaffold00003_27_28_833_F | >scaffold00003_27_28_833_F | ||
GTACTCGCCGACTTTCCCGTTCTCATC | GTACTCGCCGACTTTCCCGTTCTCATC | ||
>scaffold00003_27_28_474_R | >scaffold00003_27_28_474_R | ||
GTACACCTCGTGCTCGGGACACAAATGGTC | GTACACCTCGTGCTCGGGACACAAATGGTC | ||
| + | |||
>scaffold00003_28_29_210_F | >scaffold00003_28_29_210_F | ||
GTACACGTGCCGTTCCCGTTTCTGGTGAAC | GTACACGTGCCGTTCCCGTTTCTGGTGAAC | ||
>scaffold00003_28_29_173_R | >scaffold00003_28_29_173_R | ||
ACGGCGTCACGAACAACACCTACTG | ACGGCGTCACGAACAACACCTACTG | ||
| + | |||
>scaffold00003_30_31_256_F | >scaffold00003_30_31_256_F | ||
TTCCGGGGATGAGTAGCCGACCAATTGTCC | TTCCGGGGATGAGTAGCCGACCAATTGTCC | ||
>scaffold00003_30_31_1239_R | >scaffold00003_30_31_1239_R | ||
GATCACCGGTTGGTAGACCAGATCCAG | GATCACCGGTTGGTAGACCAGATCCAG | ||
| + | |||
>scaffold00003_31_32_351_F | >scaffold00003_31_32_351_F | ||
CACAAATTGAACTTGAGCTGGGATGTCAGG | CACAAATTGAACTTGAGCTGGGATGTCAGG | ||
>scaffold00003_31_32_565_R | >scaffold00003_31_32_565_R | ||
CAGGATGACAGTGTGAGTCAGCCATGGAC | CAGGATGACAGTGTGAGTCAGCCATGGAC | ||
| + | |||
>scaffold00003_35_36_707_F | >scaffold00003_35_36_707_F | ||
AAGTTGAAGGAAATGGGCGAGTTCGTGAAG | AAGTTGAAGGAAATGGGCGAGTTCGTGAAG | ||
>scaffold00003_35_36_243_R | >scaffold00003_35_36_243_R | ||
CAGGTTGAACATCTCCTGGACCAGCGAAC | CAGGTTGAACATCTCCTGGACCAGCGAAC | ||
| + | |||
>scaffold00003_42_43_317_F | >scaffold00003_42_43_317_F | ||
GTTCCTGTCCTCGTGCAGCACAATTCGATC | GTTCCTGTCCTCGTGCAGCACAATTCGATC | ||
>scaffold00003_42_43_298_R | >scaffold00003_42_43_298_R | ||
ACCGGGGATCATCGTGGTCTTGTAC | ACCGGGGATCATCGTGGTCTTGTAC | ||
| + | |||
>scaffold00003_43_44_737_F | >scaffold00003_43_44_737_F | ||
ATCGTTCGCAGCGGCATCGATTCAC | ATCGTTCGCAGCGGCATCGATTCAC | ||
>scaffold00003_43_44_992_R | >scaffold00003_43_44_992_R | ||
TGAAGCTGACCAAGCTGGTGGTCTC | TGAAGCTGACCAAGCTGGTGGTCTC | ||
| + | |||
>scaffold00003_45_46_422_F | >scaffold00003_45_46_422_F | ||
GTGATGCCCTCGGATCCCAGTTCTTCGTAC | GTGATGCCCTCGGATCCCAGTTCTTCGTAC | ||
>scaffold00003_45_46_399_R | >scaffold00003_45_46_399_R | ||
AACTCGGTCAAGCTCGAGTTCCTGGTG | AACTCGGTCAAGCTCGAGTTCCTGGTG | ||
| + | |||
>scaffold00003_47_48_427_F | >scaffold00003_47_48_427_F | ||
CATCACGGCGAACAACAACCAGACCTAC | CATCACGGCGAACAACAACCAGACCTAC | ||
>scaffold00003_47_48_271_R | >scaffold00003_47_48_271_R | ||
GAACAACGGCATGAGGTCCTTGAACATGAC | GAACAACGGCATGAGGTCCTTGAACATGAC | ||
| + | |||
>scaffold00003_48_49_532_F | >scaffold00003_48_49_532_F | ||
GAAGGGTCAACAGTGAGATCGCCTGGTTC | GAAGGGTCAACAGTGAGATCGCCTGGTTC | ||
>scaffold00003_48_49_741_R | >scaffold00003_48_49_741_R | ||
TGTAGCTGCCCTTCTCCTCCTGTTC | TGTAGCTGCCCTTCTCCTCCTGTTC | ||
| + | |||
>scaffold00003_49_50_208_F | >scaffold00003_49_50_208_F | ||
GTGGTGTGCATCGTGCAGATCCCGTTCTAC | GTGGTGTGCATCGTGCAGATCCCGTTCTAC | ||
>scaffold00003_49_50_975_R | >scaffold00003_49_50_975_R | ||
AACCCGGAGCCGAAGAGTTCGAGAAAC | AACCCGGAGCCGAAGAGTTCGAGAAAC | ||
| + | |||
>scaffold00003_50_51_1445_F | >scaffold00003_50_51_1445_F | ||
TGGCGTACCTGGAGAAGGTGGTTTC | TGGCGTACCTGGAGAAGGTGGTTTC | ||
>scaffold00003_50_51_439_R | >scaffold00003_50_51_439_R | ||
CAATGGCACCCTCACATATGGCAGATAATTG | CAATGGCACCCTCACATATGGCAGATAATTG | ||
| + | |||
>scaffold00004_8_9_447_F | >scaffold00004_8_9_447_F | ||
GAACCTGCTGTTCATGGTCGGTCTG | GAACCTGCTGTTCATGGTCGGTCTG | ||
>scaffold00004_8_9_234_R | >scaffold00004_8_9_234_R | ||
ACAGTGGTCTCCGTCTACAGTCCAC | ACAGTGGTCTCCGTCTACAGTCCAC | ||
| + | |||
>scaffold00004_12_13_750_F | >scaffold00004_12_13_750_F | ||
TCGATGGACATGCTCTCCTCCGAGATCGTC | TCGATGGACATGCTCTCCTCCGAGATCGTC | ||
>scaffold00004_12_13_895_R | >scaffold00004_12_13_895_R | ||
TATGCGAGCGTCCGAAAAAGATCGACCAGC | TATGCGAGCGTCCGAAAAAGATCGACCAGC | ||
| + | |||
>scaffold00004_13_14_550_F | >scaffold00004_13_14_550_F | ||
GAGCACATCAAGATCACTGACAATGGGATC | GAGCACATCAAGATCACTGACAATGGGATC | ||
>scaffold00004_13_14_243_R | >scaffold00004_13_14_243_R | ||
AACACTCCGAAATCCGCTGGCTCTC | AACACTCCGAAATCCGCTGGCTCTC | ||
| + | |||
>scaffold00004_14_15_556_F | >scaffold00004_14_15_556_F | ||
CTGGTGGGACTCGTTGAGCATCCAATG | CTGGTGGGACTCGTTGAGCATCCAATG | ||
>scaffold00004_14_15_220_R | >scaffold00004_14_15_220_R | ||
CCGAGGGAAAGAACCGGACATTGTG | CCGAGGGAAAGAACCGGACATTGTG | ||
| + | |||
>scaffold00004_15_16_1515_F | >scaffold00004_15_16_1515_F | ||
CTTCACCGGCCTGTTCTTCGTCATCAC | CTTCACCGGCCTGTTCTTCGTCATCAC | ||
>scaffold00004_15_16_167_R | >scaffold00004_15_16_167_R | ||
CAGTACCTGTGGCGTCACACGCGATAAGAG | CAGTACCTGTGGCGTCACACGCGATAAGAG | ||
| + | |||
>scaffold00004_16_17_236_F | >scaffold00004_16_17_236_F | ||
GGTTCGACACCAGGATTTCGAGGTCAC | GGTTCGACACCAGGATTTCGAGGTCAC | ||
>scaffold00004_16_17_467_R | >scaffold00004_16_17_467_R | ||
GATCTGGTTCCACATCTGTCCCTGGTG | GATCTGGTTCCACATCTGTCCCTGGTG | ||
| + | |||
>scaffold00004_18_19_226_F | >scaffold00004_18_19_226_F | ||
GTGTGATCGACACGGTTACGTCGAAG | GTGTGATCGACACGGTTACGTCGAAG | ||
>scaffold00004_18_19_492_R | >scaffold00004_18_19_492_R | ||
TTAACAGGGGGCCCTTCCTTCAACACGGTC | TTAACAGGGGGCCCTTCCTTCAACACGGTC | ||
| + | |||
>scaffold00004_20_21_2075_F | >scaffold00004_20_21_2075_F | ||
AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG | AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG | ||
>scaffold00004_20_21_521_R | >scaffold00004_20_21_521_R | ||
TGAAGATCACCCAGGATCTCAAGGAGTACG | TGAAGATCACCCAGGATCTCAAGGAGTACG | ||
| + | |||
>scaffold00004_21_22_386_F | >scaffold00004_21_22_386_F | ||
GAAGACTTCGCCACCGAATACCTCAGC | GAAGACTTCGCCACCGAATACCTCAGC | ||
>scaffold00004_21_22_182_R | >scaffold00004_21_22_182_R | ||
TTCGACGTACACACCACCCTCGCCGTAATC | TTCGACGTACACACCACCCTCGCCGTAATC | ||
| + | |||
>scaffold00004_23_24_720_F | >scaffold00004_23_24_720_F | ||
TCCACCGGAACATGGGTGATACTCGAC | TCCACCGGAACATGGGTGATACTCGAC | ||
>scaffold00004_23_24_552_R | >scaffold00004_23_24_552_R | ||
GTTGCCTTTGCACAGGTAGATCCCG | GTTGCCTTTGCACAGGTAGATCCCG | ||
| + | |||
>scaffold00004_24_25_667_F | >scaffold00004_24_25_667_F | ||
CAAGCCATGTCGAAGCAGCACTACTC | CAAGCCATGTCGAAGCAGCACTACTC | ||
>scaffold00004_24_25_322_R | >scaffold00004_24_25_322_R | ||
AAGCACCTGCTGTTCACAAGCGAACTGATC | AAGCACCTGCTGTTCACAAGCGAACTGATC | ||
| + | |||
>scaffold00004_25_26_542_F | >scaffold00004_25_26_542_F | ||
GCAGATCGACGAGTACCTGGAGTTCTAC | GCAGATCGACGAGTACCTGGAGTTCTAC | ||
>scaffold00004_25_26_247_R | >scaffold00004_25_26_247_R | ||
AGGAGTCCGACCATGAAGATCAGGAAGATC | AGGAGTCCGACCATGAAGATCAGGAAGATC | ||
| + | |||
>scaffold00004_29_30_278_F | >scaffold00004_29_30_278_F | ||
CATTTGTGACACCTGTCCAGCAGTACG | CATTTGTGACACCTGTCCAGCAGTACG | ||
>scaffold00004_29_30_264_R | >scaffold00004_29_30_264_R | ||
TCTGGTGAGCCGACAGGTATTCCTC | TCTGGTGAGCCGACAGGTATTCCTC | ||
| + | |||
| + | >scf1_10_11_191_R | ||
| + | ATTCCTCGCGCAGTTGACCCAGTTC | ||
| + | >scf1_10_11_533_F | ||
| + | TTCGCCACCACCGGTCTAGGATGAGAGATC | ||
| + | |||
| + | >scf1_13_14_1221_R | ||
| + | AGTCGTCGAAGCGCAGAAGGTACATGTAGG | ||
| + | >scf1_13_14_961_F | ||
| + | GTCATCGGACAGCCGATTCTTCGACGACTG | ||
| + | |||
| + | >scf1_14_15_1407_F | ||
| + | CTTGTTTCGATAGCATTACCGCCCAGTAAC | ||
| + | >scf1_14_15_533_R | ||
| + | GTACTCGTCGAAGGTGGACACGTTCACTC | ||
| + | |||
| + | >scf1_16_17_1785_F | ||
| + | CTCGAAGACGTCAACAACCACACCAATCTG | ||
| + | >scf1_16_17_478_R | ||
| + | CTCGTAACTCTCCATCGGTTCTCCAGTAAC | ||
| + | |||
| + | >scf1_22_23_238_F | ||
| + | GTTTCACTCATCGGTGTACCCTCCCGATC | ||
| + | >scf1_22_23_244_R | ||
| + | GAAGTTCGTCATCGAGAACGTCTCCGTGC | ||
| + | |||
| + | >scf1_24_25_444_F | ||
| + | ATTCGACGGGCAGGACGTCAACAAG | ||
| + | >scf1_24_25_458_R | ||
| + | TAGGTGTGCTCCAGGTAGGTGAGGAAC | ||
| + | |||
| + | >scf1_26_27_369_F | ||
| + | ATGAGCGAGCAGGTCTTCCTCCTAC | ||
| + | >scf1_26_27_706_R | ||
| + | GAACACGCCGTAGTTGTTCAGCGTGAAG | ||
| + | |||
| + | >scf1_29_30_1643_F | ||
| + | CCGATCCAGGCGAAGATCAGATTGGTC | ||
| + | >scf1_29_30_767_R | ||
| + | ATCGACATAGCGCTCCAACAGATCGGTGAC | ||
| + | |||
| + | >scf1_32_33_169_R | ||
| + | TTGTCAATGTAGCGTTGACGGGTGCCTGAC | ||
| + | >scf1_32_33_266_F | ||
| + | AACATTGACAGGGTGGCACGCAGTTCG | ||
| + | |||
| + | >scf1_33_34_365_R | ||
| + | GTTCCCTGCTGCTGAACTGGTTCTTCAC | ||
| + | >scf1_33_34_795_F | ||
| + | GTCGACGACGAGAATCCGGTTCATG | ||
| + | |||
| + | >scf1_37_38_498_F | ||
| + | TTCGACGAACTGTGGCAACGCTTCTCC | ||
| + | >scf1_37_38_562_R | ||
| + | AGGACGTCATGCACGGTCAACACAC | ||
| + | |||
| + | >scf1_40_41_522_F | ||
| + | GACTACAAGCCGGAGAACGTGCTCATCTC | ||
| + | >scf1_40_41_660_R | ||
| + | GTGCAGAAGGGATAGAGGGTGACGTAC | ||
| + | |||
| + | >scf1_43_44_440_F | ||
| + | CTCGGAGGAAAACGGTGAACAGTCAGCAAC | ||
| + | >scf1_43_44_563_R | ||
| + | TGAACGCCTCCACCCAGCATTTGATCACAG | ||
| + | |||
| + | >scf1_44_45_485_R | ||
| + | GTGTTGCGATCGGATTCGTAGCTGAC | ||
| + | >scf1_44_45_93801_F | ||
| + | ACACTGCTGCCGCCACGGTTGACTGT | ||
| + | |||
| + | >scf1_46_47_370_F | ||
| + | GTAAACTCCTGATCTCAGCCGCTCTCGTG | ||
| + | >scf1_46_47_845_R | ||
| + | CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG | ||
| + | |||
| + | >scf1_53_54_414_F | ||
| + | TTCTGGAAGACCATCTGCAGACCGATCTCG | ||
| + | >scf1_53_54_823_R | ||
| + | TACGCGAAGGGACGTTAGAGATGGCAC | ||
| + | |||
| + | >scf1_54_55_1250_F | ||
| + | CTGGAACCGGATGATGTTGGAGTCCTC | ||
| + | >scf1_54_55_175_R | ||
| + | TACCGAGGAACTGATCGACCTCTCC | ||
| + | |||
| + | >scf1_56_57_184_R | ||
| + | CAAACCGGTGGTCGGGCTGATCATGTATTC | ||
| + | >scf1_56_57_412_F | ||
| + | GTACTCACGATGGTCCTTCTCCTCGTTG | ||
| + | |||
| + | >scf1_59_60_1979_F | ||
| + | GTGACCAGCCAGGTCTGTTCGAATCTCAC | ||
| + | >scf1_59_60_625_R | ||
| + | GAGATCATGTACGCCGACTTCAGCTTCGTG | ||
| + | |||
| + | >scf1_63_64_1286_R | ||
| + | CGATAGGTGAGACTCGATGCATGGTTAGTG | ||
| + | >scf1_63_64_711_F | ||
| + | ACGTAGTTCCCGACCGTAGAGCAGTTC | ||
| + | |||
| + | >scf1_66_67_346_R | ||
| + | ACTGTTCCTGCTGTACTCCTCGGTG | ||
| + | >scf1_66_67_428_F | ||
| + | CTTCGTCACTGGCTGTGTCAAGGTCAAC | ||
| + | |||
| + | >scf1_6_7_870_R | ||
| + | CGATCTCCACGTTTGACAGGATGCTCTG | ||
| + | >scf1_6_7_939_F | ||
| + | ATCAGCGGTTACGTCTGGTGGTGAC | ||
| + | |||
| + | >scf1_7_8_545_R | ||
| + | ATCAGTTTGATCGGCGAGACGTGCGAGACC | ||
| + | >scf1_7_8_818_F | ||
| + | GAACTGGAGTTGATGGAGCAGCGCGAAAC | ||
| + | |||
| + | >scf3_12_13_2070_F | ||
| + | CTCCAGCAGGGTTTCATCGATTCGGTAG | ||
| + | >scf3_12_13_324_R | ||
| + | CTGGACCAGTTCACCCTCCAGATCATG | ||
| + | |||
| + | >scf3_18_19_471_R | ||
| + | GTATACCGCAACATCGGCGAAATGAAGTC | ||
| + | >scf3_18_19_746_F | ||
| + | GTTGCTTCGAATCTGGTGACGGGAG | ||
| + | |||
| + | >scf3_20_21_200_R | ||
| + | GAAGATCGGCAGCTCCATCGTCTCATC | ||
| + | >scf3_20_21_294_F | ||
| + | ATCAGCCCCGAGCAATTGACCGACCTGAAC | ||
| + | |||
| + | >scf3_21_22_1034_F | ||
| + | GAAGTAACCGGTGGTGTTTTCGGCCAG | ||
| + | >scf3_21_22_354_R | ||
| + | ATCTCGCCGGTTACAAGCAGATCCTC | ||
| + | |||
| + | >scf3_22_23_1299_F | ||
| + | TTCAGATGGTTAAGGACAGCTTCATGAGCG | ||
| + | >scf3_22_23_368_R | ||
| + | ACGTGATCGGTGGGAACTTCGACTC | ||
| + | |||
| + | >scf3_25_26_360_F | ||
| + | GAAAGGCTGACCATCTACCCGGAGTAC | ||
| + | >scf3_25_26_636_R | ||
| + | TGATCCACGTGTCCGTACATCATCGTG | ||
| + | |||
| + | >scf3_33_34_1969_F | ||
| + | GTCCTCAAGCATCGCAAGCGCAAGGAAAAG | ||
| + | >scf3_33_34_485_R | ||
| + | CTCCAACTGCTCGGAGAGGAACTTGTAAC | ||
| + | |||
| + | >scf3_34_35_222_R | ||
| + | ATCTCCGCAGTCATATCGGTACGGG | ||
| + | >scf3_34_35_491_F | ||
| + | GAACGAGACCAGGAGACATAGTGGCGAGTC | ||
| + | |||
| + | >scf3_36_37_355_F | ||
| + | CTGCTCTTCCTCGGCATGCTCATGTTC | ||
| + | >scf3_36_37_430_R | ||
| + | ATAGCCCGCCCACTGCATGACAAAC | ||
| + | |||
| + | >scf3_38_39_613_R | ||
| + | GTACGAGCAGTTCACCAGTTGCGTG | ||
| + | >scf3_38_39_791_F | ||
| + | AACTGTCGACGAAGAACCGCGTCATGAGAG | ||
| + | |||
| + | >scf3_39_40_333_R | ||
| + | CTGATCCGTCATGGCGCACATTCCACGATG | ||
| + | >scf3_39_40_351_F | ||
| + | CACTTGTCTTCTCCTCCGGATACCTGG | ||
| + | |||
| + | >scf3_44_45_1117_F | ||
| + | CTCTGTGAGTACGTGGAACAGGCGATG | ||
| + | >scf3_44_45_224_R | ||
| + | AAGATCACCACGGCGTCGACACAGCTCAAC | ||
| + | |||
| + | >scf3_6_7_245_R | ||
| + | GCATGCTGTTGTTGATGCTGTCCTTCAG | ||
| + | >scf3_6_7_918_F | ||
| + | ATGCTCAGTCCGGGAGTACAGCTCGGTAAC | ||
| + | |||
| + | >scf3_9_10_212_R | ||
| + | TTTCCGTTGAGCCGCCACTCGTACTTGTAG | ||
| + | >scf3_9_10_376_F | ||
| + | CATGCACAAGCAGGACCTCATCGAG | ||
| + | |||
| + | >scf4_19_20_273_F | ||
| + | GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC | ||
| + | >scf4_19_20_878_R | ||
| + | GACGAGCAACTGGACTCGCTGAACAAG | ||
| + | |||
| + | >scf4_26_27_255_F | ||
| + | GATCGTTCGACTACCCACAATCCGCAGATC | ||
| + | >scf4_26_27_671_R | ||
| + | GGTAGAGCAGCCATTCCAGTTCCTGTG | ||
| + | |||
| + | >scf4_27_28_485_R | ||
| + | GAAAACGGGGTGTTCCAGCTTCATCGCTTC | ||
| + | >scf4_27_28_577_F | ||
| + | CTGTTCACCGAGGAGGGCGAGTTCTTCTTC | ||
| + | |||
| + | >scf4_28_29_1409_F | ||
| + | AACCGACACACCTGCAGTTGCCGTACCAAC | ||
| + | >scf4_28_29_207_R | ||
| + | TACTCCGGGATAAGCAAGGCTGAGCTG | ||
| + | |||
| + | >scf4_30_31_444_F | ||
| + | ACCTTCTTGCTCTTACGGCCCACCTTG | ||
| + | >scf4_30_31_739_R | ||
| + | GAGTACGTCAAGTTCATTTTCGCCACCAC | ||
| + | |||
| + | >scf3_1_2_276_F | ||
| + | GACGTGTACATCTCGAACATCTCGCGG | ||
| + | >scf3_1_2_387_R | ||
| + | AAAACGGGCGTTCACCGCATGGTAC | ||
| + | </pre> | ||
Latest revision as of 16:12, 19 February 2011
Contents |
Primer Naming Format
Gap primer format
- >[scaffold #]_[Front Contig #]_[Back Contig #]_[primer postion from Gap(Ns)(bp)]_[Forward/Reverse]
ex) >scaffold00001_1_2_1105_F, >scaffold00001_1_2_529_R
CONTIG 1 CONTIG 2
------>
5' 3'
--------CTCAGGTCAGGCAGGCGTGATGATCTCTTC----------------NNNNNNNNN----------------------------------------
GTTAGAGGAGAGTGCGGCTGGGCTCCAATCAT
3' 5'
<-------
Positions: 1105bp 529bp
List of primer
- By Using Primer3
- "Can't find proper primer" means Primer3 couldn't found primer which satisfies several conditions (length, GC contents, Tm, complementarity between primers and complementarity between chromosome and 3' of primer).
primers
- 115 gaps (230 primers)
>scaffold00001_2_3_751_F CAGAACTGGAGAACGTCCATGCTCGAGAAC >scaffold00001_2_3_1360_R TCGATCACCAGCTCACGGAAGTCCTCGTTG >scaffold00001_3_4_586_F GTCAGTGCCGATCAGGATGTTGTCCAC >scaffold00001_3_4_183_R ATCAACTGCCAGAACTCGTCGGTCCTCATG >scaffold00001_4_5_568_F TACGTCGAGGAACTGGGCGACTTCAAGACC >scaffold00001_4_5_838_R CATGTCGTCGATCTGCACGTTGACCAG >scaffold00001_9_10_1135_F AGACCGGATTGAGTGCTTCCTCTGCTGGTG >scaffold00001_9_10_1408_R CGACCAATTTCCGACGAGGTGACATTC >scaffold00001_11_12_354_F AAGGTCAGGATGGCGGTGAACACCTTGGTC >scaffold00001_11_12_345_R ATGTTGTCGCTGACCTTCGCGATACGGGAG >scaffold00001_12_13_1313_F TTTCAGGCTTTGAGGATCGAGGTAAACAGG >scaffold00001_12_13_315_R TCGCATCTTCTCAGCCAGTTCCGAG >scaffold00001_15_16_126613_F GTTCCCGGTTGCGATCGGTGAGGAT >scaffold00001_15_16_569_R CTTCTACCACGACACACACACCGATCC >scaffold00001_17_18_225_F GATCGTGACGAACGTGAAGGTCCAC >scaffold00001_17_18_408_R CAGATCCGGTACAGCTCCTTGTATTCCAG >scaffold00001_18_19_320_F CAGATAGATGGTGAAGTCAGGACGACGG >scaffold00001_18_19_177_R ATGAACTTTCCACCGATGCCGGTCGTCTTC >scaffold00001_21_22_243_F AAGGACGGACGTGGACATCGATCAGCAATC >scaffold00001_21_22_1379_R AGGACTCCTGTACGTCGATCACGACGAGTG >scaffold00001_23_24_410_F ACTGACCACGGTGCAGCTCTACTTCAC >scaffold00001_23_24_1184_R AGCGTGTAGTCGGCGGTATACACCTTC >scaffold00001_25_26_203_F GTCGAGGGGATCAACACAAGTGGAATCAAG >scaffold00001_25_26_184_R GTACCGTCGACAAGCAACTCCTGATCGTC >scaffold00001_27_28_280_F TGACGTGAGGGGACTACGGATGTAC >scaffold00001_27_28_260_R ACTTCGACAGGTAGGCCTTGCAGAG >scaffold00001_31_32_566_F AAACGAGCCGTACAACGGGCAACTG >scaffold00001_31_32_169_R ATCTTCCTGACCTCGTCGGTGTGGTACTTC >scaffold00001_34_35_1071_F CAGTACGTCATCTTCGACCGGACCATTTG >scaffold00001_34_35_2096_R AGTCGTCGTCGTCGAACATCGTGAC >scaffold00001_35_36_685_F GTCGTTCAGGTTCAGGTAGAACTCGGTGAC >scaffold00001_35_36_0_R CCCGACCGAACGGCACGCACACAA >scaffold00001_36_37_1073_F GATCGGTTTGCCGCGGTCGTTGTATTCGAC >scaffold00001_36_37_740_R ATACGGCTTCGTCGAGAACCTGACC >scaffold00001_38_39_612_F AACATGTCGTTGAGCGCGGCCAGTTTG >scaffold00001_38_39_672_R CTCTTCGACGAGATCATCCAGGTGCTG >scaffold00001_41_42_205_F ACCGAAGCAGTCGAAGACGGTACAC >scaffold00001_41_42_339_R GTCTTCTTCTGGCCCCGCAACGAGATCATG >scaffold00001_42_43_389_F TAAAATCTGTGATCTGGGCTAACCTTGGGG >scaffold00001_42_43_223_R GTGATCGGTGTGTTCGTGGCTGACCAGTAC >scaffold00001_45_46_401_F GGATCAGCTCCGGATAGTTGAAGTCGTC >scaffold00001_45_46_423_R ACAACGAGGACTTCGACAACGGCTTCAAGG >scaffold00001_47_48_1970_F GACGACTACCTGCGTAAACCCTTCGACCTG >scaffold00001_47_48_1044_R CACCAATCTGCTTGTGGGTCTGGTGCTGAC >scaffold00001_48_49_1639_F GATGTTCATCGACCTGGAGAACGTGC >scaffold00001_48_49_244_R CATCTCTCCCGTCAGCTCATCGTTCGAAG >scaffold00001_49_50_1297_F CAGGGTTTCCTGAGCCACCTTGAAC >scaffold00001_49_50_822_R CTGTTCTCCCAGGACCCGATCTTCAACTC >scaffold00001_50_51_227_F CGTGCCATGTTCTGAACGAGAACGATC >scaffold00001_50_51_860_R GTGACCAGCGAGTGAATCTTGTCGCGTTG >scaffold00001_55_56_239_F CACCTGACCTCTGACAATGAACGCCGAAAG >scaffold00001_55_56_206_R GATTGGCTTCTATACAAACTAAGGTGGCGC >scaffold00001_57_58_1204_F TCATGCTGTCCGTATCCATTTAGGACCTTC >scaffold00001_57_58_939_R TTCTACCGTGCCTACTTCGAGACCGAC >scaffold00001_61_62_285_F TCAGGTTGAGATCGACCATCGCGAG >scaffold00001_61_62_944_R TTGCACCATCAGCTCAGCATCGTCTTCGAC >scaffold00001_62_63_898_F CTGATGATCAAGGTGTTGCGGCTGTC >scaffold00001_62_63_367_R GTTGTAGCCGCCGAAGTCGTTCATGCTGAC >scaffold00001_67_68_5122_F GACCGTTGCGACCAGATATTCGGCGATT >scaffold00001_67_68_0_R AACCGCTGACCGCGTTCGTGCTGTTCT >scaffold00001_71_72_349_F AACCCACGCATCCCACTCAACGAGCTGAAC >scaffold00001_71_72_0_R TCGTTGTCCACCACGCCGACGAACA >scaffold00001_73_74_2968_F TACGCGGCAGTCGGACGGGTGTGTT >scaffold00001_73_74_0_R GCTCACCGTGCCGGTGAGGACCAA >scaffold00001_75_76_788_F GTCGACGTTTCATCACGTCACTGGGTG >scaffold00001_75_76_0_R GTGCGCCAAGACCTGCGCGAACAGT >scaffold00001_76_77_1854_F GTGATCAAGGTGATCGAAGCCCTGC >scaffold00001_76_77_535_R ATCTACAACCACCTGGGCGGCAAGGAGAAC >scaffold00001_80_81_426_F CACTGCCCAAACCGATCTATCCGACGTTC >scaffold00001_80_81_202_R GATCCAAGCTGTCTTAACGAGGAGTTGCC >scaffold00001_87_88_204_F GAAGTGATCACGCGATCCACGGGATTCCAC >scaffold00001_87_88_267_R AGCTTCCAGACCCTGGTCAACAGTC >scaffold00001_88_89_1973_F AGTAGCCGATGACCCCGTTGAGTTG >scaffold00001_88_89_463_R CAGGTGTTCAACCTCGCCCAGACCTTCTAC >scaffold00003_3_4_218_F ACAAGCTCATTGCCTACTAGATCGACTTCG >scaffold00003_3_4_1054_R GCATCGTCCAACGGTAGCCTTCTTTC >scaffold00003_7_8_365_F ATCCGACGAGTACGACATCCACCTG >scaffold00003_7_8_601_R CAGACCAACTACGCCACAAGCAAGG >scaffold00003_8_9_472_F CTGTTCCAGACCTTCCTCACCGTGCTTGAC >scaffold00003_8_9_218_R CTTATGCCCTGACAGAAGGTGCAAGATCAG >scaffold00003_10_11_1436_F TGGTCCTGCCGTACTTCCTGATCACCACTG >scaffold00003_10_11_232_R GATGGGACAGACTTCGTACACCGGTTG >scaffold00003_11_12_266_F CTGTATTGATGATCCACTCGCTGCCGCTC >scaffold00003_11_12_1184_R TACAGTGATCGGCCGTTGTTGGAGTGTGTG >scaffold00003_17_18_314_F TCTGAACGAGACGGAATGGGAGATCCTCAC >scaffold00003_17_18_222_R GACATCACCATCCAGGCAGAGAACATGCTG >scaffold00003_19_20_655_F GAAGGAACGGCAACTTTCGGTCACGGTAC >scaffold00003_19_20_485_R CAAGGAGACCGTCTACAAGATCTGGCATC >scaffold00003_23_24_235_F CCCAGGGAATATAACGATGGTTCAGGTCAG >scaffold00003_23_24_1163_R GAACTGCTGGTTCATCGAATGGTCGTTGAC >scaffold00003_24_25_268_F CTCGAAGTCCCGGTCAAGAAGATCCTCAC >scaffold00003_24_25_666_R TATCGTTCGCGTCGATGGCCGACAACATTG >scaffold00003_26_27_1270_F GAAATCAAGATCCTGAACCAGTCCCGGAAG >scaffold00003_26_27_244_R CCCGATTCCTTGTCCTGGAACAGATAGAAG >scaffold00003_27_28_833_F GTACTCGCCGACTTTCCCGTTCTCATC >scaffold00003_27_28_474_R GTACACCTCGTGCTCGGGACACAAATGGTC >scaffold00003_28_29_210_F GTACACGTGCCGTTCCCGTTTCTGGTGAAC >scaffold00003_28_29_173_R ACGGCGTCACGAACAACACCTACTG >scaffold00003_30_31_256_F TTCCGGGGATGAGTAGCCGACCAATTGTCC >scaffold00003_30_31_1239_R GATCACCGGTTGGTAGACCAGATCCAG >scaffold00003_31_32_351_F CACAAATTGAACTTGAGCTGGGATGTCAGG >scaffold00003_31_32_565_R CAGGATGACAGTGTGAGTCAGCCATGGAC >scaffold00003_35_36_707_F AAGTTGAAGGAAATGGGCGAGTTCGTGAAG >scaffold00003_35_36_243_R CAGGTTGAACATCTCCTGGACCAGCGAAC >scaffold00003_42_43_317_F GTTCCTGTCCTCGTGCAGCACAATTCGATC >scaffold00003_42_43_298_R ACCGGGGATCATCGTGGTCTTGTAC >scaffold00003_43_44_737_F ATCGTTCGCAGCGGCATCGATTCAC >scaffold00003_43_44_992_R TGAAGCTGACCAAGCTGGTGGTCTC >scaffold00003_45_46_422_F GTGATGCCCTCGGATCCCAGTTCTTCGTAC >scaffold00003_45_46_399_R AACTCGGTCAAGCTCGAGTTCCTGGTG >scaffold00003_47_48_427_F CATCACGGCGAACAACAACCAGACCTAC >scaffold00003_47_48_271_R GAACAACGGCATGAGGTCCTTGAACATGAC >scaffold00003_48_49_532_F GAAGGGTCAACAGTGAGATCGCCTGGTTC >scaffold00003_48_49_741_R TGTAGCTGCCCTTCTCCTCCTGTTC >scaffold00003_49_50_208_F GTGGTGTGCATCGTGCAGATCCCGTTCTAC >scaffold00003_49_50_975_R AACCCGGAGCCGAAGAGTTCGAGAAAC >scaffold00003_50_51_1445_F TGGCGTACCTGGAGAAGGTGGTTTC >scaffold00003_50_51_439_R CAATGGCACCCTCACATATGGCAGATAATTG >scaffold00004_8_9_447_F GAACCTGCTGTTCATGGTCGGTCTG >scaffold00004_8_9_234_R ACAGTGGTCTCCGTCTACAGTCCAC >scaffold00004_12_13_750_F TCGATGGACATGCTCTCCTCCGAGATCGTC >scaffold00004_12_13_895_R TATGCGAGCGTCCGAAAAAGATCGACCAGC >scaffold00004_13_14_550_F GAGCACATCAAGATCACTGACAATGGGATC >scaffold00004_13_14_243_R AACACTCCGAAATCCGCTGGCTCTC >scaffold00004_14_15_556_F CTGGTGGGACTCGTTGAGCATCCAATG >scaffold00004_14_15_220_R CCGAGGGAAAGAACCGGACATTGTG >scaffold00004_15_16_1515_F CTTCACCGGCCTGTTCTTCGTCATCAC >scaffold00004_15_16_167_R CAGTACCTGTGGCGTCACACGCGATAAGAG >scaffold00004_16_17_236_F GGTTCGACACCAGGATTTCGAGGTCAC >scaffold00004_16_17_467_R GATCTGGTTCCACATCTGTCCCTGGTG >scaffold00004_18_19_226_F GTGTGATCGACACGGTTACGTCGAAG >scaffold00004_18_19_492_R TTAACAGGGGGCCCTTCCTTCAACACGGTC >scaffold00004_20_21_2075_F AACTCAAGTAGACCTACCGCAAGCAGGTCGGACG >scaffold00004_20_21_521_R TGAAGATCACCCAGGATCTCAAGGAGTACG >scaffold00004_21_22_386_F GAAGACTTCGCCACCGAATACCTCAGC >scaffold00004_21_22_182_R TTCGACGTACACACCACCCTCGCCGTAATC >scaffold00004_23_24_720_F TCCACCGGAACATGGGTGATACTCGAC >scaffold00004_23_24_552_R GTTGCCTTTGCACAGGTAGATCCCG >scaffold00004_24_25_667_F CAAGCCATGTCGAAGCAGCACTACTC >scaffold00004_24_25_322_R AAGCACCTGCTGTTCACAAGCGAACTGATC >scaffold00004_25_26_542_F GCAGATCGACGAGTACCTGGAGTTCTAC >scaffold00004_25_26_247_R AGGAGTCCGACCATGAAGATCAGGAAGATC >scaffold00004_29_30_278_F CATTTGTGACACCTGTCCAGCAGTACG >scaffold00004_29_30_264_R TCTGGTGAGCCGACAGGTATTCCTC >scf1_10_11_191_R ATTCCTCGCGCAGTTGACCCAGTTC >scf1_10_11_533_F TTCGCCACCACCGGTCTAGGATGAGAGATC >scf1_13_14_1221_R AGTCGTCGAAGCGCAGAAGGTACATGTAGG >scf1_13_14_961_F GTCATCGGACAGCCGATTCTTCGACGACTG >scf1_14_15_1407_F CTTGTTTCGATAGCATTACCGCCCAGTAAC >scf1_14_15_533_R GTACTCGTCGAAGGTGGACACGTTCACTC >scf1_16_17_1785_F CTCGAAGACGTCAACAACCACACCAATCTG >scf1_16_17_478_R CTCGTAACTCTCCATCGGTTCTCCAGTAAC >scf1_22_23_238_F GTTTCACTCATCGGTGTACCCTCCCGATC >scf1_22_23_244_R GAAGTTCGTCATCGAGAACGTCTCCGTGC >scf1_24_25_444_F ATTCGACGGGCAGGACGTCAACAAG >scf1_24_25_458_R TAGGTGTGCTCCAGGTAGGTGAGGAAC >scf1_26_27_369_F ATGAGCGAGCAGGTCTTCCTCCTAC >scf1_26_27_706_R GAACACGCCGTAGTTGTTCAGCGTGAAG >scf1_29_30_1643_F CCGATCCAGGCGAAGATCAGATTGGTC >scf1_29_30_767_R ATCGACATAGCGCTCCAACAGATCGGTGAC >scf1_32_33_169_R TTGTCAATGTAGCGTTGACGGGTGCCTGAC >scf1_32_33_266_F AACATTGACAGGGTGGCACGCAGTTCG >scf1_33_34_365_R GTTCCCTGCTGCTGAACTGGTTCTTCAC >scf1_33_34_795_F GTCGACGACGAGAATCCGGTTCATG >scf1_37_38_498_F TTCGACGAACTGTGGCAACGCTTCTCC >scf1_37_38_562_R AGGACGTCATGCACGGTCAACACAC >scf1_40_41_522_F GACTACAAGCCGGAGAACGTGCTCATCTC >scf1_40_41_660_R GTGCAGAAGGGATAGAGGGTGACGTAC >scf1_43_44_440_F CTCGGAGGAAAACGGTGAACAGTCAGCAAC >scf1_43_44_563_R TGAACGCCTCCACCCAGCATTTGATCACAG >scf1_44_45_485_R GTGTTGCGATCGGATTCGTAGCTGAC >scf1_44_45_93801_F ACACTGCTGCCGCCACGGTTGACTGT >scf1_46_47_370_F GTAAACTCCTGATCTCAGCCGCTCTCGTG >scf1_46_47_845_R CTCGTCGTCTCTGGTGTAGAAGTCGTCCTG >scf1_53_54_414_F TTCTGGAAGACCATCTGCAGACCGATCTCG >scf1_53_54_823_R TACGCGAAGGGACGTTAGAGATGGCAC >scf1_54_55_1250_F CTGGAACCGGATGATGTTGGAGTCCTC >scf1_54_55_175_R TACCGAGGAACTGATCGACCTCTCC >scf1_56_57_184_R CAAACCGGTGGTCGGGCTGATCATGTATTC >scf1_56_57_412_F GTACTCACGATGGTCCTTCTCCTCGTTG >scf1_59_60_1979_F GTGACCAGCCAGGTCTGTTCGAATCTCAC >scf1_59_60_625_R GAGATCATGTACGCCGACTTCAGCTTCGTG >scf1_63_64_1286_R CGATAGGTGAGACTCGATGCATGGTTAGTG >scf1_63_64_711_F ACGTAGTTCCCGACCGTAGAGCAGTTC >scf1_66_67_346_R ACTGTTCCTGCTGTACTCCTCGGTG >scf1_66_67_428_F CTTCGTCACTGGCTGTGTCAAGGTCAAC >scf1_6_7_870_R CGATCTCCACGTTTGACAGGATGCTCTG >scf1_6_7_939_F ATCAGCGGTTACGTCTGGTGGTGAC >scf1_7_8_545_R ATCAGTTTGATCGGCGAGACGTGCGAGACC >scf1_7_8_818_F GAACTGGAGTTGATGGAGCAGCGCGAAAC >scf3_12_13_2070_F CTCCAGCAGGGTTTCATCGATTCGGTAG >scf3_12_13_324_R CTGGACCAGTTCACCCTCCAGATCATG >scf3_18_19_471_R GTATACCGCAACATCGGCGAAATGAAGTC >scf3_18_19_746_F GTTGCTTCGAATCTGGTGACGGGAG >scf3_20_21_200_R GAAGATCGGCAGCTCCATCGTCTCATC >scf3_20_21_294_F ATCAGCCCCGAGCAATTGACCGACCTGAAC >scf3_21_22_1034_F GAAGTAACCGGTGGTGTTTTCGGCCAG >scf3_21_22_354_R ATCTCGCCGGTTACAAGCAGATCCTC >scf3_22_23_1299_F TTCAGATGGTTAAGGACAGCTTCATGAGCG >scf3_22_23_368_R ACGTGATCGGTGGGAACTTCGACTC >scf3_25_26_360_F GAAAGGCTGACCATCTACCCGGAGTAC >scf3_25_26_636_R TGATCCACGTGTCCGTACATCATCGTG >scf3_33_34_1969_F GTCCTCAAGCATCGCAAGCGCAAGGAAAAG >scf3_33_34_485_R CTCCAACTGCTCGGAGAGGAACTTGTAAC >scf3_34_35_222_R ATCTCCGCAGTCATATCGGTACGGG >scf3_34_35_491_F GAACGAGACCAGGAGACATAGTGGCGAGTC >scf3_36_37_355_F CTGCTCTTCCTCGGCATGCTCATGTTC >scf3_36_37_430_R ATAGCCCGCCCACTGCATGACAAAC >scf3_38_39_613_R GTACGAGCAGTTCACCAGTTGCGTG >scf3_38_39_791_F AACTGTCGACGAAGAACCGCGTCATGAGAG >scf3_39_40_333_R CTGATCCGTCATGGCGCACATTCCACGATG >scf3_39_40_351_F CACTTGTCTTCTCCTCCGGATACCTGG >scf3_44_45_1117_F CTCTGTGAGTACGTGGAACAGGCGATG >scf3_44_45_224_R AAGATCACCACGGCGTCGACACAGCTCAAC >scf3_6_7_245_R GCATGCTGTTGTTGATGCTGTCCTTCAG >scf3_6_7_918_F ATGCTCAGTCCGGGAGTACAGCTCGGTAAC >scf3_9_10_212_R TTTCCGTTGAGCCGCCACTCGTACTTGTAG >scf3_9_10_376_F CATGCACAAGCAGGACCTCATCGAG >scf4_19_20_273_F GTCTCCTTGGTCGAGGTGCTGTTCTGAGTC >scf4_19_20_878_R GACGAGCAACTGGACTCGCTGAACAAG >scf4_26_27_255_F GATCGTTCGACTACCCACAATCCGCAGATC >scf4_26_27_671_R GGTAGAGCAGCCATTCCAGTTCCTGTG >scf4_27_28_485_R GAAAACGGGGTGTTCCAGCTTCATCGCTTC >scf4_27_28_577_F CTGTTCACCGAGGAGGGCGAGTTCTTCTTC >scf4_28_29_1409_F AACCGACACACCTGCAGTTGCCGTACCAAC >scf4_28_29_207_R TACTCCGGGATAAGCAAGGCTGAGCTG >scf4_30_31_444_F ACCTTCTTGCTCTTACGGCCCACCTTG >scf4_30_31_739_R GAGTACGTCAAGTTCATTTTCGCCACCAC >scf3_1_2_276_F GACGTGTACATCTCGAACATCTCGCGG >scf3_1_2_387_R AAAACGGGCGTTCACCGCATGGTAC
