User contributions
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- 04:08, 17 March 2014 (diff | hist) N 14.2.18 (Created page with "17일 실험 다시해서 젤 내려봄 assembly 확인 위해 transformation control이 이상해서 망함..") (top)
- 04:06, 17 March 2014 (diff | hist) N 14.2.17 (Created page with "Gibson assembly (one-step) 실험 - 직접 만든 master mixure 잘되나 실험 잘 모르겠어..") (top)
- 04:05, 17 March 2014 (diff | hist) N 14.2.14 (Created page with "control 용량을 높여서 다시내렸지만 이상함") (top)
- 04:05, 17 March 2014 (diff | hist) N 14.2.12 (Created page with "10일꺼 젤 다시 내림") (top)
- 04:05, 17 March 2014 (diff | hist) 14.2.10 (top)
- 04:04, 17 March 2014 (diff | hist) N 14.2.10 (Created page with "Reaction test BSA SrtA GFP RFPwaste Marker B+G+R S+G+R(30m,1h,2h,3h) 젤이 이상해...")
- 04:03, 17 March 2014 (diff | hist) N 14.2.6 (Created page with "standard curve 다시 쟤서 단백질 정량 srtA : 0.76-1.05 mg/ml GFP : 20.6 mRFP : 63.48-65.14 mRFP waste : 5.8 몰비율 맞춰서 대충희석해서 다음에 1대1 r...") (top)
- 04:01, 17 March 2014 (diff | hist) N 14.2.5 (Created page with "4일에 만든 standard curve로 정량 후 제대로 희석해서 PAGE 내렸으나 이상함") (top)
- 04:01, 17 March 2014 (diff | hist) m 14.2.4 (top)
- 04:00, 17 March 2014 (diff | hist) N 14.2.4 (Created page with "1. bradford정량으로 srtA, GFP,mRFP 확인해 보려고 일단 standard curve만듦 2. Double transformation")
- 03:58, 17 March 2014 (diff | hist) 14.2.3 (top)
- 03:57, 17 March 2014 (diff | hist) N 14.2.3 (Created page with "29일ㅇ[ 내린것 많아서 다 끌림 5배 희석 후 로딩 2 5 52 52 52 근데 이것도 끌림..")
- 03:56, 17 March 2014 (diff | hist) Undergraduate 유민섭
- 03:56, 17 March 2014 (diff | hist) Undergraduate 유민섭
- 07:06, 29 January 2014 (diff | hist) N 14.1.29 (Created page with "28일에 내린 15% SDS-PAGE gel 결과가 좋지 않아서 다시 내림 단백질이 너무많고, 오래 내려서 그런 것 같아서, 2람다만 내리기로함") (top)
- 07:25, 28 January 2014 (diff | hist) N 14.1.28 (Created page with "30과 10의 centricon을 이용한 단백질 농축 및 buffer change 2*Ala-GFP, mRFP1-LPETAA, srtA 농축 및 srt reaction buffer change 후 15% SDS-PAGE gel로 내려봄 ;GFP...") (top)
- 06:59, 28 January 2014 (diff | hist) Undergraduate 유민섭
- 06:59, 28 January 2014 (diff | hist) N 14.1.24 (Created page with "23일에 정제한 16도 AIM, SDS-PAGE gel 내리기 -> 성공했다고 합니다") (top)
- 06:58, 28 January 2014 (diff | hist) N 14.1.23 (Created page with "21일에 induction한 16도꺼 단백질 정제") (top)
- 06:58, 28 January 2014 (diff | hist) N 14.1.22 (Created page with "21일에 induction한 것 단백질정제 전에 bugbuster로 발현확인테스트 후 25도꺼만 단백질 정제 후 SDS-PAGE gel 내리기") (top)
- 06:56, 28 January 2014 (diff | hist) N 14.1.21 (Created page with "20일에 접종한 BL21-pJL-srtA, large scale up 및 induction ; 16도 LB 1개, 25도 AIM(Auto-Inducing Media) 1개") (top)
- 06:54, 28 January 2014 (diff | hist) N 14.1.20 (Created page with "BL21-pJL-srtA 접종") (top)
- 06:54, 28 January 2014 (diff | hist) N 14.1.17 (Created page with "16일에 induction 한 것 3가지를 단백질정제 후 PAGE gel로 내리기 -> crude, loading through, fraction, waste 이 4가지 다 해야하는데 fraction 얻었다고...") (top)
- 06:50, 28 January 2014 (diff | hist) N 14.1.16 (Created page with "15일에 접종한 BL21 3가지 large scale up 후 induction ; srtA는 16도에서 나머지는 37도에서 induction 13일에 했던 transformation 다시") (top)
- 06:48, 28 January 2014 (diff | hist) N 14.1.15 (Created page with "14일에 접종한 것 중에 BL21 3가지 다시 접종 14일에 접종한 것 중에 DH5a 4가지 에서 plasmid 추출/확인했으나 이상해서 다시 transformation 예정") (top)
- 06:46, 28 January 2014 (diff | hist) 14.1.14 (top)
- 06:45, 28 January 2014 (diff | hist) N 14.1.13 (Created page with "Filamentus bacteria phage 실험 시작 trasformation : BW25113-pATLIC-srtA; BL21(DE3)-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA; DH5a-pATLIC-srtA,-pJL-srtA,-pJL-2*Ala:GFP,-pJL-...") (top)
- 06:41, 28 January 2014 (diff | hist) Undergraduate 유민섭
- 06:48, 13 January 2014 (diff | hist) N 14.1.14 (Created page with "sortase 관련 실험!!! trasformation : BW25113-pATLIC-srtA; BL21(DE3)-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA; DH5a-pATLIC-srtA,-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA")
- 06:48, 13 January 2014 (diff | hist) Undergraduate 유민섭
- 06:47, 13 January 2014 (diff | hist) 1.14 (top)
- 06:47, 13 January 2014 (diff | hist) N 1.14 (Created page with "sortase 관련 실험!!! trasformation : BW25113-pATLIC-srtA, BL21(DE3)-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA, DH5a-pATLIC-srtA,-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA")
- 06:42, 13 January 2014 (diff | hist) Undergraduate 유민섭
- 06:21, 29 August 2013 (diff | hist) N 8.28 (Created page with "transformation한거 접종 후 plasmid prep 해서 sequencing 보내기") (top)
- 06:21, 29 August 2013 (diff | hist) Undergraduate 유민섭
- 06:20, 29 August 2013 (diff | hist) 8.27 (top)
- 06:20, 29 August 2013 (diff | hist) 8.27
- 06:19, 29 August 2013 (diff | hist) 8.27
- 06:19, 29 August 2013 (diff | hist) 8.27
- 02:47, 27 August 2013 (diff | hist) N 8.27 (Created page with "pATLIC vector enzyme cut Nacrein pcr clean up")
- 02:24, 27 August 2013 (diff | hist) Undergraduate 유민섭
- 08:21, 26 August 2013 (diff | hist) 8.26 (top)
- 07:38, 26 August 2013 (diff | hist) 8.20 (top)
- 07:36, 26 August 2013 (diff | hist) N 8.26 (Created page with "pATLIC : 단백질 표면발현벡터 File:PATLIC.png 근데 Nacrein은 원래 signal peptide가 달려있는 놈이라 이걸 떼고 클로닝햇어야했느데 안떼고...")
- 07:34, 26 August 2013 (diff | hist) N File:PATLIC.png (top)
- 07:16, 26 August 2013 (diff | hist) N 8.22 (Created page with "cloning 한거가 진짜 plasmid 맞는지 enzyme cut으로 확인 확인용이라 plamsid 4 buffer#4 0.5 EcoR1 0.5 37'c 30min") (top)
- 07:15, 26 August 2013 (diff | hist) N 8.21 (Created page with "transformation 한거 접종") (top)
- 07:15, 26 August 2013 (diff | hist) 8.20 (→PCR)
- 07:14, 26 August 2013 (diff | hist) 8.20 (→PCR)
- 07:13, 26 August 2013 (diff | hist) 8.20 (→PCR)
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