Strains

From CSBLwiki

Revision as of 03:18, 13 September 2010 (view source) Igchoi (Talk | contribs) (→Stocks@KUCSBL) ← Older edit		Latest revision as of 14:49, 26 September 2010 (view source) Igchoi (Talk | contribs) (→Stock center)
(11 intermediate revisions not shown)
Line 1:		Line 1:
	==Stock center==		==Stock center==
	===Korea===		===Korea===
-	*[http://www.genebank.go.kr/ KACC]	+	*Stock centers: [http://www.genebank.go.kr/ KACC], [http://www.brc.re.kr/_KTC/Search/M/SearchForm.aspx KCTC]
-	*[http://www.brc.re.kr/_KTC/Search/M/SearchForm.aspx KCTC]	+
	===USA===		===USA===
-	*[http://www.atcc.org ATCC]	+	*Stock centers: [http://www.atcc.org ATCC], [http://cgsc.biology.yale.edu/index.php CGSC]
-	*[http://cgsc.biology.yale.edu/index.php CGSC]	+
	==Stocks@KUCSBL==		==Stocks@KUCSBL==
	*<i>E. coli</i>		*<i>E. coli</i>
Line 12:		Line 11:
	**SURE		**SURE
	**DH10B		**DH10B
		+	***While constitutive expression of the red gamma gene has a toxic effect in DH10B (recA-) cells under some conditions, thus limiting the efficiency of recombination, tightly regulated expression of the gamma gene together with simultaneous expression of the red alpha and beta genes allows efficient homologous recombination between linear DNA fragments and BACs resident in such cells as DH10B [http://www.bioxys.com/i_openbio/pdf/genomics/Gene_bridges_manuals/BGB%201757%20Q+E%20BAC%20Modification%20Kit.pdf]
	**TOP10		**TOP10
-	**CSGC strains (Apr 2010 purchased at $50)	+	*[http://cgsc.biology.yale.edu/ CGSC] strains (Apr 2010 purchased at $50)
-	#8175 ([http://cgsc.biology.yale.edu/Strain.php?ID=86484 BW19851]): RP4-2	+	#[http://cgsc.biology.yale.edu/Strain.php?ID=86484 8175] (BW19851): RP4-2
-	#7629 (BT340)	+	#[http://cgsc.biology.yale.edu/Strain.php?ID=64645 7629] (BT340): pCP20
-	#7635 (BW25113): model strain	+	#[http://cgsc.biology.yale.edu/Strain.php?ID=64690 7635] (BW25113)
-	#7636 (BW25141): pir+	+	##<i>E. coli</i> K12
		+	##[http://ecoli.naist.jp/gb6/Resources/deletion/deletion.html Keio Collection] host strain for [http://ecoli.naist.jp/gb6/Resources/deletion/Del_construct.html gene deletion]
		+	#[http://cgsc.biology.yale.edu/Strain.php?ID=64667 7636] (BW25141)
		+	#[http://cgsc.biology.yale.edu/Strain.php?ID=5655 5478] (K27): fadD88
		+	#[http://cgsc.biology.yale.edu/Strain.php?ID=64700 7633] (BW25113/pKD46): lost
		+	#[http://cgsc.biology.yale.edu/Strain.php?ID=68099 7739] (BW25141/pKD13): lost
		+	*ordered on 9/20/2010
		+	# #7857 BW5328/pAH69
		+	# #7845 BW23474/pAH70
		+	# #7858 BW23473/pAH83
		+	# #7862 BW23473/pAH129
		+	# #7861 BW5328/pAH123
		+	# #7869 BW23474/pAH153
		+	# #12353 C600/RK2
		+	# #9796 JW2227
		+	# #7633 BW25141/pKD13
		+	# #7631 BW25141/pKD3
		+	# #7988 BW25141/pKD32
		+	# #7632 BW25141/pKD4
	==Strains info.==		==Strains info.==

---

Latest revision as of 14:49, 26 September 2010

Contents 1 Stock center 1.1 Korea 1.2 USA 2 Stocks@KUCSBL 3 Strains info.

Stock center

Korea

• Stock centers: KACC, KCTC

USA

• Stock centers: ATCC, CGSC

Stocks@KUCSBL

• E. coli
  • DH5a
  • XL1-Blue
  • SURE
  • DH10B
    • While constitutive expression of the red gamma gene has a toxic effect in DH10B (recA-) cells under some conditions, thus limiting the efficiency of recombination, tightly regulated expression of the gamma gene together with simultaneous expression of the red alpha and beta genes allows efficient homologous recombination between linear DNA fragments and BACs resident in such cells as DH10B [1]
  • TOP10
• CGSC strains (Apr 2010 purchased at $50)

1. 8175 (BW19851): RP4-2
2. 7629 (BT340): pCP20
3. 7635 (BW25113)
   1. E. coli K12
   2. Keio Collection host strain for gene deletion
4. 7636 (BW25141)
5. 5478 (K27): fadD88
6. 7633 (BW25113/pKD46): lost
7. 7739 (BW25141/pKD13): lost

• ordered on 9/20/2010

1. #7857 BW5328/pAH69
2. #7845 BW23474/pAH70
3. #7858 BW23473/pAH83
4. #7862 BW23473/pAH129
5. #7861 BW5328/pAH123
6. #7869 BW23474/pAH153
7. #12353 C600/RK2
8. #9796 JW2227
9. #7633 BW25141/pKD13
10. #7631 BW25141/pKD3
11. #7988 BW25141/pKD32
12. #7632 BW25141/pKD4

Strains info.

• E. coli genotypes - in openwetware@MIT
• Novagen E. coli manual - Novagen Competent Cells
• Choosing a competent E. coli strain
• Invitrogen Comp. Cell::Genotype]