Taq 정제방법

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sonification 해서 원심분리 후

soup만 75도에서 1시간 가열하면

Taq말고 다른 대장균단백질은 다 aggrigation되서 가라앉는다

가열 후 soup만 centricon으로 걸면 간이정제 완성!

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