Congo red

Dye decolorization

The decolorization ability of the fungal isolates for synthetic dyes in solid media was determined using the in vitro bioassay described by Novotny et al. (2004). Briefly, the fungal disk (5 mm diameters) which was removed from the pre-cultured plate was inoculated on the center of MEA media amended with 100 ppm Congo red (CR), Reactive blue 4 (RB4) or Orange II (OII). The plate was then incubated at 27℃ in the dark during 6 days, and all tests were done in triplicate. After incubation, dye decolorization was determined by the percentage of color removal rate (% CRR) on MEA media amended with CR, OII or RB4, and was expressed as +++++ (100 ≥ % CRR > 90), ++++ (90 ≥ % CRR > 70), +++ (70 ≥ % CRR > 50), ++ (50 ≥ % CRR > 30), + (30 ≥ % CRR > 0), and - (no removal).

Reference

Novotny et al. 2004. Int. Biodeter. Biodegrad. 54: 215-223