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PAHs :비극성이며 , 소수성의 유기물질로 , 화석연료와 나무를 태우는 일 , 석탄 채광, 석유 채굴 등에 의해 환경에 노출되는 오염물질이다 .
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Polycyclic aromatic hydrocarbons (PAHs), also known as poly-aromatic hydrocarbons or polynuclear aromatic hydrocarbons, are potent atmospheric pollutants that consist of fused aromatic rings and do not contain heteroatoms or carry substituents. Naphthalene is the simplest example of a PAH. PAHs occur in oil, coal, and tar deposits, and are produced as byproducts of fuel burning (whether fossil fuel or biomass). As a pollutant, they are of concern because some compounds have been identified as carcinogenic, mutagenic, and teratogenic. PAHs are also found in cooked foods. Studies have shown that high levels of PAHs are found, for example, in meat cooked at high temperatures such as grilling or barbecuing, and in smoked fish. They are also found in the interstellar medium, in comets, and in meteorites and are a candidate molecule to act as a basis for the earliest forms of life.  
 
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몇가지 물질은 돌연변이나 암을 유발 하는 물질로 알려져 있다 .
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PAHs는 2개 이상의 benzene ring 을 가진 화합물로써 , 용해 되지 않고, 안정적인 물질이다 .
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PAHs를 분해할수 있는 미생물은 산소를 ring 에 삽입하여 PAHs 의 용해도와 화학반응성을 높여 분해 할수 있다.
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2개 이상의 고리를 가지고 있는 PAHs는 물에 불용성의 물질이기 때문에 세포안으로 쉽게 들어올수 없어
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cytochrome p450 enzyme이나 dioxygense (bacteria)와 같은 intracellular enzyme 이 반응을 일으켜 분해 시키기 힘들다.
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'white rot' basidiomycetous fungi 는 extracellular enzyme system 을 가지고 있어 PAHs를 효과적으로 분해 할수 있다 .
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extracellular enzyme 은 [[lignin depolymerization]] , [[demethoxylation]] , [[decarboxylation]] , [[decarboxylation]] , [[hydroxylation]] 과
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aromatic ring opening 을 촉매 하며, 위반응들의 대부분은 oxigen radical 을 만들어 오염물질 을 공격하여 분해 하는 작용을 진행 한다.
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위 와 같이 여러개의 고리가 있는 화합물 이다.
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*PAHs 의 구조
  
 
wikipedia:http://en.wikipedia.org/wiki/Polycyclic_aromatic_hydrocarbon
 
wikipedia:http://en.wikipedia.org/wiki/Polycyclic_aromatic_hydrocarbon
  
= metabolism =
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= Metabolism =
  
 
[[file:PAHs.png]]
 
[[file:PAHs.png]]
== Non - lignolytic fungi ==
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== Non - ligninolytic fungi ==
  
[[cytochrome P450 monooxygenase]] 와 [[epoxide hydrolase]] 에 의해 trans-dihydrodiol 을 형성하거나 비효소적인 재배열을 통해
 
 
Phenol  이 되고 , conjugate 를 형성 한다 .
 
 
몇 몇 fungi 는 ''trans''-dihydrodiol 과 Phenol을 sulfation , methylation 이나 glucose,xylose,glucuronic acid 와의 conjugation 을 통해
 
대사 시킬수 있다.
 
 
*Acenaphthene : C.elegans 에 의해 6-hydroxyacenaphthenone, 1,2-acenaphthenedione, trans -1,2-dihydroxyacenaphthene, cis 1,2-dihydroxyacenaphthene으로 대사 되어진다.
 
 
 
*Fluorene : C.elegans 에 의해 9-fluorenol , 9 -fluorenone ,2-hydroxy-9-fluorenone 으로 대사 되며 , 그외 여러 fungi 도 fluorene 을 산화 시킬수 있다. (Fluorene -> 9-Fluorenol ->9-Fluorenone -> 2- Hydroxy- 9 - fluorenone )
 
 
 
*Anthracene : ''C.elegans'' 에 의해 anthracene ''trans'' -1,2 - dihydrodiol enantiomer 와 1-anthryl sulfate 로 대사 되며 이외에 ''Rhizoctonia solani'',
 
''Cryphonectria parasitica'' , ''Rhizopus arrhizus'' ,''C.echinulata'' ,''Cladosporium herbarum''에 의해 대사 되어 진다고 알려져 있다 .
 
 
 
===알려진 그룹 ===
 
 
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참고
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Reference
 
Potential of Penicillium Species in the Bioremediation
 
Potential of Penicillium Species in the Bioremediation
  
==lignolytic fungi ==
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==Ligninolytic fungi(nutrient-limited) ==
  
extracellular lignolytic enzyme에 의한 non specific radical oxidation 에 의해 분해된다.  
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Lignolytic fungi degrade PAHs trough non specific radical oxidation by Extracellular lignolytic enzymes.
 
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* Extracellular lignolytic enzyme
* extracellular lignolytic enzyme
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::*[[Lignin peroxidase]]
::*[[lignin peroxidase]]
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::*[[Manganese peroxidase | Maganese-dependent peroxidase]]
::*[[manganese peroxidase | maganese-dependent peroxidase]]
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::*[[Manganese-independent peroxidase]]
::*[[manganese-independent peroxidase]]
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::*[[Laccase]]
::*[[laccase]]
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[[file:Ligninolytic fungi.png| 600px]]
 
[[file:Ligninolytic fungi.png| 600px]]
  
출처 :Fungal metabolism of polycyclic aromatic hydrocarbons: past,
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reference :Fungal metabolism of polycyclic aromatic hydrocarbons: past,
 
present and future applications in bioremediation
 
present and future applications in bioremediation
  
=== 알려진 group ===
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=== Known species  ===
 
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* Phanerochaete chrysosprium
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::[[KUC Phanerochaete | phanerochaete ]]
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* ''Phanerochaete chrysosprium''
  
* Trametes versicolor
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* ''Trametes versicolor''
  
::[[KUC Trametes versicolor | trametes versicolor ]]
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::[[KUC Trametes versicolor | Trametes versicolor ]]
  
*Pleurotus ostreatus  
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*''Pleurotus ostreatus''
  
::[[KUC Pleurotus ostreatus | pleurotus ostreatus ]]
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::[[KUC Pleurotus ostreatus | Pleurotus ostreatus ]]

Latest revision as of 16:31, 8 April 2013

Contents

Polycyclic aromatic hydrocarbons (PAHs), also known as poly-aromatic hydrocarbons or polynuclear aromatic hydrocarbons, are potent atmospheric pollutants that consist of fused aromatic rings and do not contain heteroatoms or carry substituents. Naphthalene is the simplest example of a PAH. PAHs occur in oil, coal, and tar deposits, and are produced as byproducts of fuel burning (whether fossil fuel or biomass). As a pollutant, they are of concern because some compounds have been identified as carcinogenic, mutagenic, and teratogenic. PAHs are also found in cooked foods. Studies have shown that high levels of PAHs are found, for example, in meat cooked at high temperatures such as grilling or barbecuing, and in smoked fish. They are also found in the interstellar medium, in comets, and in meteorites and are a candidate molecule to act as a basis for the earliest forms of life.



  • PAHs 의 구조

wikipedia:http://en.wikipedia.org/wiki/Polycyclic_aromatic_hydrocarbon

[edit] Metabolism

PAHs.png

[edit] Non - ligninolytic fungi

Class Genus
Zygomycetes cunninghamella blakesleeana , M verrucosa
C.ehinulata ,Mucor racemosus
C.elgans 외 3 Genus
Ascomycetes Cryphonectria parasitica ,Neurospora crassa 외 4 Genus
Blastomycetes Candida krusei , Rhodotorula gutinis 외 5 Genus
Hyphomycetes Aspergillus niger , Monosporium olivaceum 외 30 Genus
Coelomycetes Cinnobolus cesatii , C,sporulosum 외 3 Genus


Reference Potential of Penicillium Species in the Bioremediation

[edit] Ligninolytic fungi(nutrient-limited)

Lignolytic fungi degrade PAHs trough non specific radical oxidation by Extracellular lignolytic enzymes.

  • Extracellular lignolytic enzyme


Oxidation of polycyclic aromatic hydrocarbons by Ligninolytic fungi Ligninolytic fungi.png

reference :Fungal metabolism of polycyclic aromatic hydrocarbons: past, present and future applications in bioremediation

[edit] Known species

  • Phanerochaete chrysosprium
  • Trametes versicolor
Trametes versicolor
  • Pleurotus ostreatus
Pleurotus ostreatus