14.4.11

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Latest revision as of 07:30, 20 July 2014

1.pSB1C3 다시 접종

2. 10일에 gel prep 한 srt A gene cluster 를 template로

PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol

PCR for pEKEx2-srtA gene cluster cloning into pSB1C3 with Q5 pol

<조성>

rxn buffer 10

dNTP 1

F.primer 2.5

R.primer 2.5

Template 1

Q5 pol 0.5

Q5 GC 10

DW 22.5

up to 50

<condition>

98 30

-98 10

68 15'- *30

72 10' 16 --

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