14.4.16

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Revision as of 07:41, 20 July 2014 (view source) Guest (Talk | contribs) (Created page with "PCR srt A gene cluster for gibson assembly with Q5 pol PCR pEKEx2 for gibson assembly with Q5 pol template : genomic DNA, pEKEx2 <조성> rxn buffer 10 dNTP 1 F.primer 2.5 ...") ← Older edit		Revision as of 07:42, 20 July 2014 (view source) Guest (Talk | contribs) Newer edit →
Line 24:		Line 24:
	up to 50		up to 50
-	<condition-srtA>                     <condition-pEKEx2>	+	<condition-srtA>
-	98 30''                                        98 30''	+	98 30''
-	-98 10'                                     -98 10'	+	-98 10'
-	55,60,65 30''                            68 15'-*35	+	55,60,65 30''
-	72 5'-*35                                  72 10'	+	72 5'-*35
-	72 10'                                        16 --	+	72 10'
	16--		16--
-	실패                                         성공-gel prep	+	실패
		+
		+	<condition-pEKEx2>
		+
		+	98 30''
		+
		+	-98 10'
		+
		+	68 15'-*35
		+
		+	72 10'
		+
		+	16 --
		+
		+	성공-gel prep

---

Revision as of 07:42, 20 July 2014

PCR srt A gene cluster for gibson assembly with Q5 pol PCR pEKEx2 for gibson assembly with Q5 pol

template : genomic DNA, pEKEx2

<조성>

rxn buffer 10

dNTP 1

F.primer 2.5

R.primer 2.5

Template 2

Q5 pol 0.5

Q5 GC 10

DW 21.5

up to 50

<condition-srtA>

98 30

-98 10'

55,60,65 30

72 5'-*35

72 10'

16--

실패

<condition-pEKEx2>

98 30

-98 10'

68 15'-*35

72 10'

16 --

성공-gel prep