14.4.16
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(Difference between revisions)
Line 44: | Line 44: | ||
98 30'' | 98 30'' | ||
- | + | -98 10' | |
68 15'-*35 | 68 15'-*35 |
Latest revision as of 07:42, 20 July 2014
PCR srt A gene cluster for gibson assembly with Q5 pol PCR pEKEx2 for gibson assembly with Q5 pol
template : genomic DNA, pEKEx2
<조성>
rxn buffer 10
dNTP 1
F.primer 2.5
R.primer 2.5
Template 2
Q5 pol 0.5
Q5 GC 10
DW 21.5
up to 50
<condition-srtA>
98 30
-98 10'
55,60,65 30
72 5'-*35
72 10'
16--
실패
<condition-pEKEx2>
98 30
-98 10'
68 15'-*35
72 10'
16 --
성공-gel prep