14.4.16
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PCR srt A gene cluster for gibson assembly with Q5 pol PCR pEKEx2 for gibson assembly with Q5 pol
template : genomic DNA, pEKEx2
<조성>
rxn buffer 10
dNTP 1
F.primer 2.5
R.primer 2.5
Template 2
Q5 pol 0.5
Q5 GC 10
DW 21.5
up to 50
<condition-srtA> <condition-pEKEx2>
98 30 98 30
-98 10' -98 10'
55,60,65 30 68 15'-*35
72 5'-*35 72 10'
72 10' 16 --
16--
실패 성공-gel prep