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(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)- 08:29, 23 June 2014 14.4.7 (hist) [297 bytes] Guest (Talk | contribs) (Created page with "srtA gene cluster for pSB1C3 cloning PCR with Q5 pol <조성> Q5 rxn buffer : 10 dNTP : 1 F.R primer : 2.5 Template : 2 Q5 pol : 0.5 Q5 GC : 10 DW : 21.5 up to 50 < P...")
- 08:24, 23 June 2014 14.4.2 (hist) [80 bytes] Guest (Talk | contribs) (Created page with "pSB1c3-srtA gene cluster cloning 할 때 쓸 srtA gene cluster PCR primer 주문")
- 08:22, 23 June 2014 14.4.1 (hist) [24 bytes] Guest (Talk | contribs) (Created page with "srtA gene cluster 확인")
- 08:22, 23 June 2014 14.3.31 (hist) [26 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 , pEKEx2 size check")
- 08:21, 23 June 2014 14.3.29 (hist) [21 bytes] Guest (Talk | contribs) (Created page with "pEKEx2 plasmid 추출")
- 08:19, 23 June 2014 14.3.28 (hist) [64 bytes] Guest (Talk | contribs) (Created page with "Oneshot-pEKEx2 : LB 3ml 접종 Oneshot-pCLTon2 : transformation Agar")
- 08:18, 23 June 2014 14.3.27 (hist) [86 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 plasmid 추출 Oneshot-pEKEx2 : transformation Spectinomycin 배지 만들기")
- 08:17, 23 June 2014 14.3.26 (hist) [273 bytes] Guest (Talk | contribs) (Created page with "Oneshot-pSB1C3 : LB 3ml 접종 Uniprot에서 Agarase pili에 적합한 agarase 알아보기 N,C terminal 쪽에 catabolytic domain 없이 잘 빠져나와있고, monomer에 b-...")
- 08:13, 23 June 2014 14.3.25 (hist) [50 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 증폭위해 Oneshot-pSB1C3 transformation")
- 02:10, 2 April 2014 2014 ComGen Course (hist) [788 bytes] Mbnmbn00 (Talk | contribs) (Created page with "=2014 spring Comgen Course= ==Info== Textbook: :[http://www.amazon.com/Biological-Sequence-Analysis-Probabilistic-Proteins/dp/0521629713] Biological Sequence Analysis Lecture: :[...")
- 12:16, 26 March 2014 Agarse screening (hist) [144 bytes] Guest (Talk | contribs) (Created page with "http://www.uniprot.org/uniprot/?query=job%3a2014032662AJZWU6QE&format=tab&columns=id,entry%20name,reviewed,protein%20names,genes,organism,length")
- 04:18, 17 March 2014 Taq 정제방법 (hist) [220 bytes] Guest (Talk | contribs) (Created page with "sonification 해서 원심분리 후 soup만 75도에서 1시간 가열하면 Taq말고 다른 대장균단백질은 다 aggrigation되서 가라앉는다 가열 후 soup만 c...")
- 04:16, 17 March 2014 14.3.13 (hist) [64 bytes] Guest (Talk | contribs) (Created page with "호구 GFP 희석않고 reaction test 했더니 다 끌림...")
- 04:15, 17 March 2014 14.3.12 (hist) [29 bytes] Guest (Talk | contribs) (Created page with "11일에 배양한 GFP 정제")
- 04:15, 17 March 2014 14.3.11 (hist) [114 bytes] Guest (Talk | contribs) (Created page with "10일에 접종한거 AIM에 배양 호구 GFP로 reaction test 했으나 GFP가 생각보다 농도가 옅다??")
- 04:14, 17 March 2014 14.3.10 (hist) [31 bytes] Guest (Talk | contribs) (Created page with "Bl21-2*Ala:GFP만 다시 접종")
- 04:13, 17 March 2014 14.3.6 (hist) [78 bytes] Guest (Talk | contribs) (Created page with "GFP 10배 희석, mRFP 30배 희석해서 PAGE걸었는데 gfp가 이상하다")
- 04:13, 17 March 2014 14.3.5 (hist) [70 bytes] Guest (Talk | contribs) (Created page with "4일에 농축한것 정량 srtA-2.09 mg/ml GFP-25.1 mRFP-63.5-74.4")
- 04:12, 17 March 2014 14.3.4 (hist) [39 bytes] Guest (Talk | contribs) (Created page with "새로 정제한 srtA, GFP,mRFP 농축")
- 04:12, 17 March 2014 14.2.26 (hist) [204 bytes] Guest (Talk | contribs) (Created page with "25일에 induction한거 cell harvest, sonification, centrifuge 후 soup만 75도에서 1시간 가열, 원심분리 후 soup만 centricon으로 농축 - Taq 정제 25일에 ...")
- 04:10, 17 March 2014 14.2.25 (hist) [77 bytes] Guest (Talk | contribs) (Created page with "24일에 접종한거 1L large scale up 후 induction streaking한거 접종")
- 04:09, 17 March 2014 14.2.24 (hist) [93 bytes] Guest (Talk | contribs) (Created page with "Taq Pol DIY로 만들기 프로젝트 Taq 클로닝 된거 plate에 streaking lb에 접종")
- 04:08, 17 March 2014 14.2.21 (hist) [27 bytes] Guest (Talk | contribs) (Created page with "srtA,mRFP,GFP 다시 정제")
- 04:08, 17 March 2014 14.2.18 (hist) [118 bytes] Guest (Talk | contribs) (Created page with "17일 실험 다시해서 젤 내려봄 assembly 확인 위해 transformation control이 이상해서 망함..")
- 04:06, 17 March 2014 14.2.17 (hist) [102 bytes] Guest (Talk | contribs) (Created page with "Gibson assembly (one-step) 실험 - 직접 만든 master mixure 잘되나 실험 잘 모르겠어..")
- 04:05, 17 March 2014 14.2.14 (hist) [57 bytes] Guest (Talk | contribs) (Created page with "control 용량을 높여서 다시내렸지만 이상함")
- 04:05, 17 March 2014 14.2.12 (hist) [26 bytes] Guest (Talk | contribs) (Created page with "10일꺼 젤 다시 내림")
- 04:04, 17 March 2014 14.2.10 (hist) [121 bytes] Guest (Talk | contribs) (Created page with "Reaction test BSA SrtA GFP RFPwaste Marker B+G+R S+G+R(30m,1h,2h,3h) 젤이 이상해...")
- 04:03, 17 March 2014 14.2.6 (hist) [200 bytes] Guest (Talk | contribs) (Created page with "standard curve 다시 쟤서 단백질 정량 srtA : 0.76-1.05 mg/ml GFP : 20.6 mRFP : 63.48-65.14 mRFP waste : 5.8 몰비율 맞춰서 대충희석해서 다음에 1대1 r...")
- 04:01, 17 March 2014 14.2.5 (hist) [94 bytes] Guest (Talk | contribs) (Created page with "4일에 만든 standard curve로 정량 후 제대로 희석해서 PAGE 내렸으나 이상함")
- 04:00, 17 March 2014 14.2.4 (hist) [113 bytes] Guest (Talk | contribs) (Created page with "1. bradford정량으로 srtA, GFP,mRFP 확인해 보려고 일단 standard curve만듦 2. Double transformation")
- 03:57, 17 March 2014 14.2.3 (hist) [104 bytes] Guest (Talk | contribs) (Created page with "29일ㅇ[ 내린것 많아서 다 끌림 5배 희석 후 로딩 2 5 52 52 52 근데 이것도 끌림..")
- 07:06, 29 January 2014 14.1.29 (hist) [167 bytes] Guest (Talk | contribs) (Created page with "28일에 내린 15% SDS-PAGE gel 결과가 좋지 않아서 다시 내림 단백질이 너무많고, 오래 내려서 그런 것 같아서, 2람다만 내리기로함")
- 07:25, 28 January 2014 14.1.28 (hist) [360 bytes] Guest (Talk | contribs) (Created page with "30과 10의 centricon을 이용한 단백질 농축 및 buffer change 2*Ala-GFP, mRFP1-LPETAA, srtA 농축 및 srt reaction buffer change 후 15% SDS-PAGE gel로 내려봄 ;GFP...")
- 06:59, 28 January 2014 14.1.24 (hist) [81 bytes] Guest (Talk | contribs) (Created page with "23일에 정제한 16도 AIM, SDS-PAGE gel 내리기 -> 성공했다고 합니다")
- 06:58, 28 January 2014 14.1.23 (hist) [47 bytes] Guest (Talk | contribs) (Created page with "21일에 induction한 16도꺼 단백질 정제")
- 06:58, 28 January 2014 14.1.22 (hist) [144 bytes] Guest (Talk | contribs) (Created page with "21일에 induction한 것 단백질정제 전에 bugbuster로 발현확인테스트 후 25도꺼만 단백질 정제 후 SDS-PAGE gel 내리기")
- 06:56, 28 January 2014 14.1.21 (hist) [115 bytes] Guest (Talk | contribs) (Created page with "20일에 접종한 BL21-pJL-srtA, large scale up 및 induction ; 16도 LB 1개, 25도 AIM(Auto-Inducing Media) 1개")
- 06:54, 28 January 2014 14.1.20 (hist) [20 bytes] Guest (Talk | contribs) (Created page with "BL21-pJL-srtA 접종")
- 06:54, 28 January 2014 14.1.17 (hist) [400 bytes] Guest (Talk | contribs) (Created page with "16일에 induction 한 것 3가지를 단백질정제 후 PAGE gel로 내리기 -> crude, loading through, fraction, waste 이 4가지 다 해야하는데 fraction 얻었다고...")
- 06:50, 28 January 2014 14.1.16 (hist) [156 bytes] Guest (Talk | contribs) (Created page with "15일에 접종한 BL21 3가지 large scale up 후 induction ; srtA는 16도에서 나머지는 37도에서 induction 13일에 했던 transformation 다시")
- 06:48, 28 January 2014 14.1.15 (hist) [180 bytes] Guest (Talk | contribs) (Created page with "14일에 접종한 것 중에 BL21 3가지 다시 접종 14일에 접종한 것 중에 DH5a 4가지 에서 plasmid 추출/확인했으나 이상해서 다시 transformation 예정")
- 06:45, 28 January 2014 14.1.13 (hist) [190 bytes] Guest (Talk | contribs) (Created page with "Filamentus bacteria phage 실험 시작 trasformation : BW25113-pATLIC-srtA; BL21(DE3)-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA; DH5a-pATLIC-srtA,-pJL-srtA,-pJL-2*Ala:GFP,-pJL-...")
- 06:48, 13 January 2014 14.1.14 (hist) [182 bytes] Guest (Talk | contribs) (Created page with "sortase 관련 실험!!! trasformation : BW25113-pATLIC-srtA; BL21(DE3)-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA; DH5a-pATLIC-srtA,-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA")
- 06:47, 13 January 2014 1.14 (hist) [175 bytes] Guest (Talk | contribs) (Created page with "sortase 관련 실험!!! trasformation : BW25113-pATLIC-srtA, BL21(DE3)-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA, DH5a-pATLIC-srtA,-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA")
- 06:21, 29 August 2013 8.28 (hist) [74 bytes] Guest (Talk | contribs) (Created page with "transformation한거 접종 후 plasmid prep 해서 sequencing 보내기")
- 02:47, 27 August 2013 8.27 (hist) [396 bytes] Guest (Talk | contribs) (Created page with "pATLIC vector enzyme cut Nacrein pcr clean up")
- 07:36, 26 August 2013 8.26 (hist) [320 bytes] Guest (Talk | contribs) (Created page with "pATLIC : 단백질 표면발현벡터 File:PATLIC.png 근데 Nacrein은 원래 signal peptide가 달려있는 놈이라 이걸 떼고 클로닝햇어야했느데 안떼고...")
- 07:16, 26 August 2013 8.22 (hist) [159 bytes] Guest (Talk | contribs) (Created page with "cloning 한거가 진짜 plasmid 맞는지 enzyme cut으로 확인 확인용이라 plamsid 4 buffer#4 0.5 EcoR1 0.5 37'c 30min")
- 07:15, 26 August 2013 8.21 (hist) [28 bytes] Guest (Talk | contribs) (Created page with "transformation 한거 접종")