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(Latest | Earliest) View (newer 20 | older 20) (20 | 50 | 100 | 250 | 500)- 07:51, 20 July 2014 14.5.2 (hist) [40 bytes] Guest (Talk | contribs) (Created page with "Oneshot-gibson product : electroporation")
- 07:50, 20 July 2014 14.5.1 (hist) [61 bytes] Guest (Talk | contribs) (Created page with "2ml 접종한것 nutrient broth 100ml에 배양 -안자라..")
- 07:50, 20 July 2014 14.4.29 (hist) [71 bytes] Guest (Talk | contribs) (Created page with "''Corynebacterium glutamicum'' comp cell 만들기 -2 ml LB 에 접종")
- 07:49, 20 July 2014 14.4.28 (hist) [43 bytes] Guest (Talk | contribs) (Created page with "25일에 gibson transformation한거 접종")
- 07:48, 20 July 2014 14.4.25 (hist) [119 bytes] Guest (Talk | contribs) (Created page with "gibson assembly 다시 (2* master mix) 후 transformation : 콜로리 떳다. pCLTon2 tranformation -안되...엉엉")
- 07:46, 20 July 2014 14.4.24 (hist) [248 bytes] Guest (Talk | contribs) (Created page with "1. streptomycin LB plate 만들기 2. gibson assembly(2* master mix) experiment : pilin 9 : pEKEx2 1 + mix 10 control(positive) : 5 + mix 5 assembly 된거 바로 ''E.coli'' ...")
- 07:41, 20 July 2014 14.4.16 (hist) [639 bytes] Guest (Talk | contribs) (Created page with "PCR srt A gene cluster for gibson assembly with Q5 pol PCR pEKEx2 for gibson assembly with Q5 pol template : genomic DNA, pEKEx2 <조성> rxn buffer 10 dNTP 1 F.primer 2.5 ...")
- 07:34, 20 July 2014 14.4.15 (hist) [320 bytes] Guest (Talk | contribs) (Created page with "PCR srt A gene cluser for gibson assembly and cloning into pSB1C3 with Q5 pol template <조성> rxn buffer 10 dNTP 1 F.primer 2.5 R.primer 2.5 Template 2 Q5 pol 0.5 Q...")
- 07:32, 20 July 2014 14.4.14 (hist) [259 bytes] Guest (Talk | contribs) (Created page with "PCR srt A gene cluster for gibson assembly with Q5 pol <조성> rxn buffer 10 dNTP 1 F.primer 2.5 R.primer 2.5 Template 2 Q5 pol 0.5 Q5 GC 10 DW 22.5 up to 50 <conditi...")
- 07:29, 20 July 2014 14.4.11 (hist) [398 bytes] Guest (Talk | contribs) (Created page with "1.pSB1C3 다시 접종 2. 10일에 gel prep 한 srt A gene cluster 를 template로 PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol PCR for pEKEx2-srtA gene cl...")
- 13:09, 24 June 2014 14.4.12 (hist) [13 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 추출")
- 08:40, 23 June 2014 14.4.10 (hist) [569 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 다시 접종 PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol")
- 08:34, 23 June 2014 14.4.8 (hist) [378 bytes] Guest (Talk | contribs) (Created page with "보다 증폭잘된다는 TAKARA LA Taq 사용 1. srtA gene cluster for pSB1C3 cloning PCR with LA Taq <조성> LA Taq 0.5 Rxn buffer 5 MgCl2 5 dNTP 8 F,R Primer 4 Templa...")
- 08:29, 23 June 2014 14.4.7 (hist) [297 bytes] Guest (Talk | contribs) (Created page with "srtA gene cluster for pSB1C3 cloning PCR with Q5 pol <조성> Q5 rxn buffer : 10 dNTP : 1 F.R primer : 2.5 Template : 2 Q5 pol : 0.5 Q5 GC : 10 DW : 21.5 up to 50 < P...")
- 08:24, 23 June 2014 14.4.2 (hist) [80 bytes] Guest (Talk | contribs) (Created page with "pSB1c3-srtA gene cluster cloning 할 때 쓸 srtA gene cluster PCR primer 주문")
- 08:22, 23 June 2014 14.4.1 (hist) [24 bytes] Guest (Talk | contribs) (Created page with "srtA gene cluster 확인")
- 08:22, 23 June 2014 14.3.31 (hist) [26 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 , pEKEx2 size check")
- 08:21, 23 June 2014 14.3.29 (hist) [21 bytes] Guest (Talk | contribs) (Created page with "pEKEx2 plasmid 추출")
- 08:19, 23 June 2014 14.3.28 (hist) [64 bytes] Guest (Talk | contribs) (Created page with "Oneshot-pEKEx2 : LB 3ml 접종 Oneshot-pCLTon2 : transformation Agar")
- 08:18, 23 June 2014 14.3.27 (hist) [86 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 plasmid 추출 Oneshot-pEKEx2 : transformation Spectinomycin 배지 만들기")