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(Latest | Earliest) View (newer 20 | older 20) (20 | 50 | 100 | 250 | 500)- 07:13, 26 August 2013 8.20 (hist) [956 bytes] Guest (Talk | contribs) (Created page with "pB4가 pJL보다 발현 protein의 solubility를 높여준다. DpN2 : TEMP과 VECTOR의 항생제저항성이 같을 때만 사용한다. 항생제저항성이 다르면 쓸 ...")
- 07:02, 26 August 2013 8.19 (hist) [191 bytes] Guest (Talk | contribs) (Created page with "==pcr== 500mb 짜리 temp 1 -plasmid니깐 10x buffer 1 dNTP 1 primer 1 HQ 5 DW 36 a-Taq ...")
- 05:53, 14 August 2013 8.14 (hist) [634 bytes] Guest (Talk | contribs) (Created page with "pB4 가 transformation이 안됬다 ==Transformation== BL21(DE3) - pB4 DHA-pB4, pRBAOK")
- 07:56, 13 August 2013 8.13 (hist) [297 bytes] Guest (Talk | contribs) (Created page with "Nacrein , Nacrein CA, Nacrein DR, Nacrein R 접종 Transformation DH5a(증폭 및 컨트롤)-pB4, pB2 BL21(DE3)-pB4,pJL(T7 promoter가 있슴) BW2113-pAT(pBAD가 있슴)")
- 03:31, 12 August 2013 LIC 법 (hist) [1,850 bytes] Guest (Talk | contribs) (Created page with "http://bitesizebio.com/articles/get-your-clone-90-of-the-time-with-ligation-independent-cloning/ Ligation Independent Cloning 으로서 conventional cloning 처럼 제한효...")
- 03:08, 12 August 2013 Cloning (hist) [40 bytes] Guest (Talk | contribs) (Created page with "LIC")
- 03:31, 2 August 2013 8.2 (hist) [116 bytes] Guest (Talk | contribs) (Created page with "==TLC solvent만들기== 당 확인용 solvent 3 : 2 : 2 = n-butanol(1-butanol) : ethanol : water (9:6:6, 6:4:4)")
- 03:30, 2 August 2013 TLC (hist) [506 bytes] Guest (Talk | contribs) (Created page with "==TLC(Thin Layer Chromatography)== File:TLC.jpg 1. 이렇게 설정하고, 농도가 적은 sample은 뿌리고 말리고 다시 뿌리고를 반복 7회 정도 2. solve...")
- 02:51, 2 August 2013 8.1 (hist) [435 bytes] Guest (Talk | contribs) (Created page with "TLC(Thin Layer Chromatography)")
- 02:40, 25 July 2013 Electroporation용 (hist) [447 bytes] Guest (Talk | contribs) (Created page with "1. 2x LB에 접종해서 OD 0.6-0.8정도 까지 키우고 얼음에 20분 방치 2. sorvall tube로 옮겨 담고, 3600rpm, 4'C 5min 원심분리 3. cell down된 pellet을 gly...")
- 02:40, 25 July 2013 Gel extraction kit (hist) [398 bytes] Guest (Talk | contribs) (Created page with "원리는 그냥 DNA 추출이랑 비슷 전기영동 후 원하는 부분을 잘라서 젤을 녹인 후 실리카겔에 통과시켜 dna만 남게한 후 washing, elution 해...")
- 02:39, 25 July 2013 7.24 (hist) [910 bytes] Guest (Talk | contribs) (Created page with "==Electroporation comp cell 만들기== 1. 2x LB에 접종해서 OD 0.6-0.8정도 까지 키우고 얼음에 20분 방치 2. sorvall tube로 옮겨 담고, 3600rpm, 4'C 5min 원...")
- 02:52, 24 July 2013 PCR Clean up (hist) [239 bytes] Guest (Talk | contribs) (Created page with "1. 컬럼에 5x volume CB과 pcr한거를 넣고 원심분리 1min 2. NW 750 원심분리 1min + 1min 3. 에탄올 날라가기 기다리기 1min 4. 1.5ml tube로 갈아끼...")
- 02:51, 24 July 2013 7.9 (hist) [256 bytes] Guest (Talk | contribs) (Created page with "==PCR CLEAN UP== 1. 컬럼에 5x volume CB과 pcr한거를 넣고 원심분리 1min 2. NW 750 원심분리 1min + 1min 3. 에탄올 날라가기 기다리기 1min 4. 1.5ml ...")
- 02:46, 24 July 2013 곰팡이 rna 추출 kit (hist) [351 bytes] Guest (Talk | contribs) (Created page with "==곰팡이에서 RNA 추출 kit== 1. 액체질소를 붓고 막자사발로 cell을 grinding 2. RLC 3.lilac 컬럼 원심분리 2min 4. 걸러져 나온 거 + 0.5 volume...")
- 02:45, 24 July 2013 7.23 (hist) [386 bytes] Guest (Talk | contribs) (Created page with "==곰팡이에서 RNA 추출== 1. 액체질소를 붓고 막자사발로 cell을 grinding 2. RLC 3.lilac 컬럼 원심분리 2min 4. 걸러져 나온 거 + 0.5 volume의 1...")
- 06:39, 23 July 2013 RNA 추출 (hist) [111 bytes] Guest (Talk | contribs) (Created page with "http://en.wikipedia.org/wiki/Guanidinium_thiocyanate-phenol-chloroform_extraction")
- 07:41, 22 July 2013 라용언 introduction (hist) [12 bytes] Guest (Talk | contribs) (Created page with "으앙구닌")
- 03:22, 22 July 2013 7.19 (hist) [384 bytes] Guest (Talk | contribs) (Created page with "==PAGE gel만들기== 단백질의 크기가 클수록 낮은 %의 gel을 사용한다. 준비물 : running gel과 stocking gel stocking gel을 좀 더 늦게 만든다-APS랑 ...")
- 02:14, 19 July 2013 Ampicilin (hist) [340 bytes] Guest (Talk | contribs) (Created page with "http://en.wikipedia.org/wiki/Ampicillin 참조 페니실린계 항생제, beta-lactam antibiotic penetrate Gram-positive and some Gram-negative bacteria 세포벽을 합성...")