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(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)- 07:13, 26 August 2013 8.20 (hist) [956 bytes] Guest (Talk | contribs) (Created page with "pB4가 pJL보다 발현 protein의 solubility를 높여준다. DpN2 : TEMP과 VECTOR의 항생제저항성이 같을 때만 사용한다. 항생제저항성이 다르면 쓸 ...")
- 07:02, 26 August 2013 8.19 (hist) [191 bytes] Guest (Talk | contribs) (Created page with "==pcr== 500mb 짜리 temp 1 -plasmid니깐 10x buffer 1 dNTP 1 primer 1 HQ 5 DW 36 a-Taq ...")
- 05:53, 14 August 2013 8.14 (hist) [634 bytes] Guest (Talk | contribs) (Created page with "pB4 가 transformation이 안됬다 ==Transformation== BL21(DE3) - pB4 DHA-pB4, pRBAOK")
- 07:56, 13 August 2013 8.13 (hist) [297 bytes] Guest (Talk | contribs) (Created page with "Nacrein , Nacrein CA, Nacrein DR, Nacrein R 접종 Transformation DH5a(증폭 및 컨트롤)-pB4, pB2 BL21(DE3)-pB4,pJL(T7 promoter가 있슴) BW2113-pAT(pBAD가 있슴)")
- 03:31, 12 August 2013 LIC 법 (hist) [1,850 bytes] Guest (Talk | contribs) (Created page with "http://bitesizebio.com/articles/get-your-clone-90-of-the-time-with-ligation-independent-cloning/ Ligation Independent Cloning 으로서 conventional cloning 처럼 제한효...")
- 03:08, 12 August 2013 Cloning (hist) [40 bytes] Guest (Talk | contribs) (Created page with "LIC")
- 03:31, 2 August 2013 8.2 (hist) [116 bytes] Guest (Talk | contribs) (Created page with "==TLC solvent만들기== 당 확인용 solvent 3 : 2 : 2 = n-butanol(1-butanol) : ethanol : water (9:6:6, 6:4:4)")
- 03:30, 2 August 2013 TLC (hist) [506 bytes] Guest (Talk | contribs) (Created page with "==TLC(Thin Layer Chromatography)== File:TLC.jpg 1. 이렇게 설정하고, 농도가 적은 sample은 뿌리고 말리고 다시 뿌리고를 반복 7회 정도 2. solve...")
- 02:51, 2 August 2013 8.1 (hist) [435 bytes] Guest (Talk | contribs) (Created page with "TLC(Thin Layer Chromatography)")
- 02:40, 25 July 2013 Electroporation용 (hist) [447 bytes] Guest (Talk | contribs) (Created page with "1. 2x LB에 접종해서 OD 0.6-0.8정도 까지 키우고 얼음에 20분 방치 2. sorvall tube로 옮겨 담고, 3600rpm, 4'C 5min 원심분리 3. cell down된 pellet을 gly...")
- 02:40, 25 July 2013 Gel extraction kit (hist) [398 bytes] Guest (Talk | contribs) (Created page with "원리는 그냥 DNA 추출이랑 비슷 전기영동 후 원하는 부분을 잘라서 젤을 녹인 후 실리카겔에 통과시켜 dna만 남게한 후 washing, elution 해...")
- 02:39, 25 July 2013 7.24 (hist) [910 bytes] Guest (Talk | contribs) (Created page with "==Electroporation comp cell 만들기== 1. 2x LB에 접종해서 OD 0.6-0.8정도 까지 키우고 얼음에 20분 방치 2. sorvall tube로 옮겨 담고, 3600rpm, 4'C 5min 원...")
- 02:52, 24 July 2013 PCR Clean up (hist) [239 bytes] Guest (Talk | contribs) (Created page with "1. 컬럼에 5x volume CB과 pcr한거를 넣고 원심분리 1min 2. NW 750 원심분리 1min + 1min 3. 에탄올 날라가기 기다리기 1min 4. 1.5ml tube로 갈아끼...")
- 02:51, 24 July 2013 7.9 (hist) [256 bytes] Guest (Talk | contribs) (Created page with "==PCR CLEAN UP== 1. 컬럼에 5x volume CB과 pcr한거를 넣고 원심분리 1min 2. NW 750 원심분리 1min + 1min 3. 에탄올 날라가기 기다리기 1min 4. 1.5ml ...")
- 02:46, 24 July 2013 곰팡이 rna 추출 kit (hist) [351 bytes] Guest (Talk | contribs) (Created page with "==곰팡이에서 RNA 추출 kit== 1. 액체질소를 붓고 막자사발로 cell을 grinding 2. RLC 3.lilac 컬럼 원심분리 2min 4. 걸러져 나온 거 + 0.5 volume...")
- 02:45, 24 July 2013 7.23 (hist) [386 bytes] Guest (Talk | contribs) (Created page with "==곰팡이에서 RNA 추출== 1. 액체질소를 붓고 막자사발로 cell을 grinding 2. RLC 3.lilac 컬럼 원심분리 2min 4. 걸러져 나온 거 + 0.5 volume의 1...")
- 06:39, 23 July 2013 RNA 추출 (hist) [111 bytes] Guest (Talk | contribs) (Created page with "http://en.wikipedia.org/wiki/Guanidinium_thiocyanate-phenol-chloroform_extraction")
- 07:41, 22 July 2013 라용언 introduction (hist) [12 bytes] Guest (Talk | contribs) (Created page with "으앙구닌")
- 03:22, 22 July 2013 7.19 (hist) [384 bytes] Guest (Talk | contribs) (Created page with "==PAGE gel만들기== 단백질의 크기가 클수록 낮은 %의 gel을 사용한다. 준비물 : running gel과 stocking gel stocking gel을 좀 더 늦게 만든다-APS랑 ...")
- 02:14, 19 July 2013 Ampicilin (hist) [340 bytes] Guest (Talk | contribs) (Created page with "http://en.wikipedia.org/wiki/Ampicillin 참조 페니실린계 항생제, beta-lactam antibiotic penetrate Gram-positive and some Gram-negative bacteria 세포벽을 합성...")
- 02:11, 19 July 2013 Kanamycin (hist) [332 bytes] Guest (Talk | contribs) (Created page with "http://en.wikipedia.org/wiki/Kanamycin 참조 Kanamycin interacts with the 30S subunit of prokaryotic ribosomes. It induces substantial amounts of mistranslation and indirectly...")
- 09:38, 18 July 2013 Arabinose operon (hist) [379 bytes] Guest (Talk | contribs) (Created page with "아라비도스 대사에 관련있는 구조유전자 BAD를 KO 시킥, 원하는 유전자를 넣은 VECTOR를 만듦 아라비도스를 넣어주어 오페론이 ACTIVATION...")
- 09:30, 18 July 2013 전기영동 (hist) [1,167 bytes] Guest (Talk | contribs) (Created page with "EtBr을 녹인 액체한천을 전기영동 틀애 붓고 comb을 이용해서 14개의 wall을 만든다. 그 위에 젤이 잠기도록 버퍼를 붓는다. ×5 loading dye (lo...")
- 09:22, 18 July 2013 단백질발현실험 (hist) [3,511 bytes] Guest (Talk | contribs) (Created page with "==단백질발현실험== 5. cell harvest : large scale - 500ml bottle 두개에 옮겨 담아서 원심분리 후 냉동보관 cell harvest : small scale - 500 마이크로L...")
- 09:19, 18 July 2013 Sma1 (hist) [74 bytes] Guest (Talk | contribs) (Created page with "Sma1(20U/ul) 1 플라스미드 44 버퍼 5 up to 50")
- 09:19, 18 July 2013 Restriction enzyme (hist) [8 bytes] Guest (Talk | contribs) (Created page with "Sma1")
- 09:18, 18 July 2013 LB (hist) [470 bytes] Guest (Talk | contribs) (Created page with "==LB broth== 100ml : LB 2.5g ==LB agar== 100ml : LB 2.5g, agar 1.5g agar가 용량의 1.5%보다 작으면 묽어져서 spreading 할 때 배지가 백금이에 파일 수가...")
- 09:16, 18 July 2013 MMR (hist) [169 bytes] Guest (Talk | contribs) (Created page with "MMG Agar(100ml) Sdl 배지 2.3 % sea salt 0.1 % yeast extract 0.05 % Ammonium Cloride 50mM Tris-HCl, pH 7.4(최종) up to 100ml DW 1.5g Agar")
- 09:16, 18 July 2013 배지 (hist) [15 bytes] Guest (Talk | contribs) (Created page with "MMR")
- 09:12, 18 July 2013 플라스미드 추출 kit (hist) [692 bytes] Guest (Talk | contribs) (Created page with "=플라스미드 추출= 1. 형질전환 된 cell 3ml를 항생제가 첨가된 LB broth에 현탁 배양")
- 09:11, 18 July 2013 곰팡이 GDNA 추출 (hist) [261 bytes] Guest (Talk | contribs) (Created page with "액화질소처리 후 막자사발로 cell을 물리적으로 먼저 깨고 lysis buffer에 녹인 후 chloroform : Isoamylalchol 유기용매로 수용성(DNA)와 지용성(...")
- 09:10, 18 July 2013 DNA 추출 (hist) [81 bytes] Guest (Talk | contribs) (Created page with "곰팡이 GDNA 추출")
- 09:10, 18 July 2013 Prep kit 원리 (hist) [791 bytes] Guest (Talk | contribs) (Created page with "Slica gel에 DNA가 binding 하는 이유 참고) http://en.wikipedia.org/wiki/DNA_separation_by_silica_adsorption Binding to silica surfaces in the presence of certain salts an...")
- 09:09, 18 July 2013 Alpha-Taq pol (hist) [546 bytes] Guest (Talk | contribs) (Created page with "==Reaction mixture== 10x alpha-Taq reaction buffer 5 uL dNTP mix(2.5mM) 3 uL primer 2 uL templete-genomic DNA 3 u...")
- 09:06, 18 July 2013 PCR (hist) [519 bytes] Guest (Talk | contribs) (Created page with "reaction buffer는 무조건 5 dNTP mix는 1kb당 1 혹은 +1 prime도 2 Templete - copy수가 많은 plasmid DNA의 경우 1 Templete - genomic DNA의 경우 3 alp...")
- 09:03, 18 July 2013 TSS용 (hist) [1,500 bytes] Guest (Talk | contribs) (Created page with "=competent cell 만들기= 준비물 : TSS solution 50ml(85% LB 1.0625g, 10% Polyethylene glycol 5g, 5% DMSO 2.5ml, 50mM MgCl2 anhydrous 0.228g), 2×LB medium 200ml (LB 6.2g, Na...")
- 09:03, 18 July 2013 Competent cell 만들기 (hist) [34 bytes] Guest (Talk | contribs) (Created page with "TSS Electroporation용")
- 09:02, 18 July 2013 TSS (hist) [976 bytes] Guest (Talk | contribs) (Created page with "1. -80°C에서 얼렸던 competent cell을 ice에 넣고 녹인다. ice cold competent cell 50마이크로L와 8.8ng/마이크로L짜리 PUC19 플라스미드 DNA 1마이크로L...")
- 09:01, 18 July 2013 Electroporation (hist) [821 bytes] Guest (Talk | contribs) (Created page with "전기충격으로 membrane을 흐물흐물하게 만든 후 DNA를 transformation하는 방법으로, cell이 죽기 쉬우나 efficiency가 높다. 과전류가 흘러서 cell...")
- 09:01, 18 July 2013 Transformation (hist) [28 bytes] Guest (Talk | contribs) (Created page with "TSS Electroporation")
- 08:58, 18 July 2013 7.18 (hist) [841 bytes] Guest (Talk | contribs) (Created page with "==Electroporation== 전기충격으로 membrane을 흐물흐물하게 만든 후 DNA를 transformation하는 방법으로, cell이 죽기 쉬우나 efficiency가 높다. 과전...")
- 08:13, 18 July 2013 PET Expression System (hist) [458 bytes] Guest (Talk | contribs) (Created page with "http://www.bio.davidson.edu/Courses/Molbio/MolStudents/spring2003/Causey/pET.html")
- 08:06, 18 July 2013 항생제 (hist) [28 bytes] Guest (Talk | contribs) (Created page with "ampicilin kanamycin")
- 08:05, 18 July 2013 과발현법 (hist) [47 bytes] Guest (Talk | contribs) (Created page with "Arabinose operon pET Expression System")
- 08:00, 18 July 2013 공부 (hist) [80 bytes] Guest (Talk | contribs) (Created page with "항생제 과발현법")
- 05:52, 17 July 2013 7.17 (hist) [571 bytes] Guest (Talk | contribs) (Created page with "==PCR== reaction buffer는 무조건 5 dNTP mix는 1kb당 1 혹은 +1 prime도 2 Templete - copy수가 많은 plasmid DNA의 경우 1 - genomic DNA의 경우 3...")
- 05:44, 17 July 2013 7.16 (hist) [145 bytes] Guest (Talk | contribs) (Created page with "==항생제 stock 만들기== 항생제 물에 녹이고 멸균필터처리 autoclave 돌리면 항생제 으앙쥬금되니깐 멸균필터로")
- 05:33, 15 July 2013 7.15 (hist) [645 bytes] Guest (Talk | contribs) (Created page with "==colony PCR== 1. 20ul DW에 colony를 적당량 섞고 2. 95'C에서 5분 3. centrifuge maxi 3분 4. soup을 templete로 4ul 5. alpha-Taq PCR")
- 04:57, 8 July 2013 7.8 (hist) [857 bytes] Guest (Talk | contribs) (0)
- 06:06, 25 April 2013 CRISPR (hist) [417 bytes] Igchoi (Talk | contribs) (Created page with "==Readings== [http://www.sciencemag.org/content/339/6121/768.summary Science] [http://elife.elifesciences.org/content/2/e00563.abstract eLife]")