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(Latest | Earliest) View (newer 20 | older 20) (20 | 50 | 100 | 250 | 500)- 09:11, 18 July 2013 곰팡이 GDNA 추출 (hist) [261 bytes] Guest (Talk | contribs) (Created page with "액화질소처리 후 막자사발로 cell을 물리적으로 먼저 깨고 lysis buffer에 녹인 후 chloroform : Isoamylalchol 유기용매로 수용성(DNA)와 지용성(...")
- 09:10, 18 July 2013 DNA 추출 (hist) [81 bytes] Guest (Talk | contribs) (Created page with "곰팡이 GDNA 추출")
- 09:10, 18 July 2013 Prep kit 원리 (hist) [791 bytes] Guest (Talk | contribs) (Created page with "Slica gel에 DNA가 binding 하는 이유 참고) http://en.wikipedia.org/wiki/DNA_separation_by_silica_adsorption Binding to silica surfaces in the presence of certain salts an...")
- 09:09, 18 July 2013 Alpha-Taq pol (hist) [546 bytes] Guest (Talk | contribs) (Created page with "==Reaction mixture== 10x alpha-Taq reaction buffer 5 uL dNTP mix(2.5mM) 3 uL primer 2 uL templete-genomic DNA 3 u...")
- 09:06, 18 July 2013 PCR (hist) [519 bytes] Guest (Talk | contribs) (Created page with "reaction buffer는 무조건 5 dNTP mix는 1kb당 1 혹은 +1 prime도 2 Templete - copy수가 많은 plasmid DNA의 경우 1 Templete - genomic DNA의 경우 3 alp...")
- 09:03, 18 July 2013 TSS용 (hist) [1,500 bytes] Guest (Talk | contribs) (Created page with "=competent cell 만들기= 준비물 : TSS solution 50ml(85% LB 1.0625g, 10% Polyethylene glycol 5g, 5% DMSO 2.5ml, 50mM MgCl2 anhydrous 0.228g), 2×LB medium 200ml (LB 6.2g, Na...")
- 09:03, 18 July 2013 Competent cell 만들기 (hist) [34 bytes] Guest (Talk | contribs) (Created page with "TSS Electroporation용")
- 09:02, 18 July 2013 TSS (hist) [976 bytes] Guest (Talk | contribs) (Created page with "1. -80°C에서 얼렸던 competent cell을 ice에 넣고 녹인다. ice cold competent cell 50마이크로L와 8.8ng/마이크로L짜리 PUC19 플라스미드 DNA 1마이크로L...")
- 09:01, 18 July 2013 Electroporation (hist) [821 bytes] Guest (Talk | contribs) (Created page with "전기충격으로 membrane을 흐물흐물하게 만든 후 DNA를 transformation하는 방법으로, cell이 죽기 쉬우나 efficiency가 높다. 과전류가 흘러서 cell...")
- 09:01, 18 July 2013 Transformation (hist) [28 bytes] Guest (Talk | contribs) (Created page with "TSS Electroporation")
- 08:58, 18 July 2013 7.18 (hist) [841 bytes] Guest (Talk | contribs) (Created page with "==Electroporation== 전기충격으로 membrane을 흐물흐물하게 만든 후 DNA를 transformation하는 방법으로, cell이 죽기 쉬우나 efficiency가 높다. 과전...")
- 08:13, 18 July 2013 PET Expression System (hist) [458 bytes] Guest (Talk | contribs) (Created page with "http://www.bio.davidson.edu/Courses/Molbio/MolStudents/spring2003/Causey/pET.html")
- 08:06, 18 July 2013 항생제 (hist) [28 bytes] Guest (Talk | contribs) (Created page with "ampicilin kanamycin")
- 08:05, 18 July 2013 과발현법 (hist) [47 bytes] Guest (Talk | contribs) (Created page with "Arabinose operon pET Expression System")
- 08:00, 18 July 2013 공부 (hist) [80 bytes] Guest (Talk | contribs) (Created page with "항생제 과발현법")
- 05:52, 17 July 2013 7.17 (hist) [571 bytes] Guest (Talk | contribs) (Created page with "==PCR== reaction buffer는 무조건 5 dNTP mix는 1kb당 1 혹은 +1 prime도 2 Templete - copy수가 많은 plasmid DNA의 경우 1 - genomic DNA의 경우 3...")
- 05:44, 17 July 2013 7.16 (hist) [145 bytes] Guest (Talk | contribs) (Created page with "==항생제 stock 만들기== 항생제 물에 녹이고 멸균필터처리 autoclave 돌리면 항생제 으앙쥬금되니깐 멸균필터로")
- 05:33, 15 July 2013 7.15 (hist) [645 bytes] Guest (Talk | contribs) (Created page with "==colony PCR== 1. 20ul DW에 colony를 적당량 섞고 2. 95'C에서 5분 3. centrifuge maxi 3분 4. soup을 templete로 4ul 5. alpha-Taq PCR")
- 04:57, 8 July 2013 7.8 (hist) [857 bytes] Guest (Talk | contribs) (0)
- 06:06, 25 April 2013 CRISPR (hist) [417 bytes] Igchoi (Talk | contribs) (Created page with "==Readings== [http://www.sciencemag.org/content/339/6121/768.summary Science] [http://elife.elifesciences.org/content/2/e00563.abstract eLife]")