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- 07:51, 20 July 2014 14.5.2 (hist) [40 bytes] Guest (Talk | contribs) (Created page with "Oneshot-gibson product : electroporation")
- 07:50, 20 July 2014 14.5.1 (hist) [61 bytes] Guest (Talk | contribs) (Created page with "2ml 접종한것 nutrient broth 100ml에 배양 -안자라..")
- 07:50, 20 July 2014 14.4.29 (hist) [71 bytes] Guest (Talk | contribs) (Created page with "''Corynebacterium glutamicum'' comp cell 만들기 -2 ml LB 에 접종")
- 07:49, 20 July 2014 14.4.28 (hist) [43 bytes] Guest (Talk | contribs) (Created page with "25일에 gibson transformation한거 접종")
- 07:48, 20 July 2014 14.4.25 (hist) [119 bytes] Guest (Talk | contribs) (Created page with "gibson assembly 다시 (2* master mix) 후 transformation : 콜로리 떳다. pCLTon2 tranformation -안되...엉엉")
- 07:46, 20 July 2014 14.4.24 (hist) [248 bytes] Guest (Talk | contribs) (Created page with "1. streptomycin LB plate 만들기 2. gibson assembly(2* master mix) experiment : pilin 9 : pEKEx2 1 + mix 10 control(positive) : 5 + mix 5 assembly 된거 바로 ''E.coli'' ...")
- 07:41, 20 July 2014 14.4.16 (hist) [639 bytes] Guest (Talk | contribs) (Created page with "PCR srt A gene cluster for gibson assembly with Q5 pol PCR pEKEx2 for gibson assembly with Q5 pol template : genomic DNA, pEKEx2 <조성> rxn buffer 10 dNTP 1 F.primer 2.5 ...")
- 07:34, 20 July 2014 14.4.15 (hist) [320 bytes] Guest (Talk | contribs) (Created page with "PCR srt A gene cluser for gibson assembly and cloning into pSB1C3 with Q5 pol template <조성> rxn buffer 10 dNTP 1 F.primer 2.5 R.primer 2.5 Template 2 Q5 pol 0.5 Q...")
- 07:32, 20 July 2014 14.4.14 (hist) [259 bytes] Guest (Talk | contribs) (Created page with "PCR srt A gene cluster for gibson assembly with Q5 pol <조성> rxn buffer 10 dNTP 1 F.primer 2.5 R.primer 2.5 Template 2 Q5 pol 0.5 Q5 GC 10 DW 22.5 up to 50 <conditi...")
- 07:29, 20 July 2014 14.4.11 (hist) [398 bytes] Guest (Talk | contribs) (Created page with "1.pSB1C3 다시 접종 2. 10일에 gel prep 한 srt A gene cluster 를 template로 PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol PCR for pEKEx2-srtA gene cl...")
- 13:09, 24 June 2014 14.4.12 (hist) [13 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 추출")
- 08:40, 23 June 2014 14.4.10 (hist) [569 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 다시 접종 PCR for pEKEx2-srtA gene cluster gibson assembly with Q5 pol")
- 08:34, 23 June 2014 14.4.8 (hist) [378 bytes] Guest (Talk | contribs) (Created page with "보다 증폭잘된다는 TAKARA LA Taq 사용 1. srtA gene cluster for pSB1C3 cloning PCR with LA Taq <조성> LA Taq 0.5 Rxn buffer 5 MgCl2 5 dNTP 8 F,R Primer 4 Templa...")
- 08:29, 23 June 2014 14.4.7 (hist) [297 bytes] Guest (Talk | contribs) (Created page with "srtA gene cluster for pSB1C3 cloning PCR with Q5 pol <조성> Q5 rxn buffer : 10 dNTP : 1 F.R primer : 2.5 Template : 2 Q5 pol : 0.5 Q5 GC : 10 DW : 21.5 up to 50 < P...")
- 08:24, 23 June 2014 14.4.2 (hist) [80 bytes] Guest (Talk | contribs) (Created page with "pSB1c3-srtA gene cluster cloning 할 때 쓸 srtA gene cluster PCR primer 주문")
- 08:22, 23 June 2014 14.4.1 (hist) [24 bytes] Guest (Talk | contribs) (Created page with "srtA gene cluster 확인")
- 08:22, 23 June 2014 14.3.31 (hist) [26 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 , pEKEx2 size check")
- 08:21, 23 June 2014 14.3.29 (hist) [21 bytes] Guest (Talk | contribs) (Created page with "pEKEx2 plasmid 추출")
- 08:19, 23 June 2014 14.3.28 (hist) [64 bytes] Guest (Talk | contribs) (Created page with "Oneshot-pEKEx2 : LB 3ml 접종 Oneshot-pCLTon2 : transformation Agar")
- 08:18, 23 June 2014 14.3.27 (hist) [86 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 plasmid 추출 Oneshot-pEKEx2 : transformation Spectinomycin 배지 만들기")
- 08:17, 23 June 2014 14.3.26 (hist) [273 bytes] Guest (Talk | contribs) (Created page with "Oneshot-pSB1C3 : LB 3ml 접종 Uniprot에서 Agarase pili에 적합한 agarase 알아보기 N,C terminal 쪽에 catabolytic domain 없이 잘 빠져나와있고, monomer에 b-...")
- 08:13, 23 June 2014 14.3.25 (hist) [50 bytes] Guest (Talk | contribs) (Created page with "pSB1C3 증폭위해 Oneshot-pSB1C3 transformation")
- 02:10, 2 April 2014 2014 ComGen Course (hist) [788 bytes] Mbnmbn00 (Talk | contribs) (Created page with "=2014 spring Comgen Course= ==Info== Textbook: :[http://www.amazon.com/Biological-Sequence-Analysis-Probabilistic-Proteins/dp/0521629713] Biological Sequence Analysis Lecture: :[...")
- 12:16, 26 March 2014 Agarse screening (hist) [144 bytes] Guest (Talk | contribs) (Created page with "http://www.uniprot.org/uniprot/?query=job%3a2014032662AJZWU6QE&format=tab&columns=id,entry%20name,reviewed,protein%20names,genes,organism,length")
- 04:18, 17 March 2014 Taq 정제방법 (hist) [220 bytes] Guest (Talk | contribs) (Created page with "sonification 해서 원심분리 후 soup만 75도에서 1시간 가열하면 Taq말고 다른 대장균단백질은 다 aggrigation되서 가라앉는다 가열 후 soup만 c...")
- 04:16, 17 March 2014 14.3.13 (hist) [64 bytes] Guest (Talk | contribs) (Created page with "호구 GFP 희석않고 reaction test 했더니 다 끌림...")
- 04:15, 17 March 2014 14.3.12 (hist) [29 bytes] Guest (Talk | contribs) (Created page with "11일에 배양한 GFP 정제")
- 04:15, 17 March 2014 14.3.11 (hist) [114 bytes] Guest (Talk | contribs) (Created page with "10일에 접종한거 AIM에 배양 호구 GFP로 reaction test 했으나 GFP가 생각보다 농도가 옅다??")
- 04:14, 17 March 2014 14.3.10 (hist) [31 bytes] Guest (Talk | contribs) (Created page with "Bl21-2*Ala:GFP만 다시 접종")
- 04:13, 17 March 2014 14.3.6 (hist) [78 bytes] Guest (Talk | contribs) (Created page with "GFP 10배 희석, mRFP 30배 희석해서 PAGE걸었는데 gfp가 이상하다")
- 04:13, 17 March 2014 14.3.5 (hist) [70 bytes] Guest (Talk | contribs) (Created page with "4일에 농축한것 정량 srtA-2.09 mg/ml GFP-25.1 mRFP-63.5-74.4")
- 04:12, 17 March 2014 14.3.4 (hist) [39 bytes] Guest (Talk | contribs) (Created page with "새로 정제한 srtA, GFP,mRFP 농축")
- 04:12, 17 March 2014 14.2.26 (hist) [204 bytes] Guest (Talk | contribs) (Created page with "25일에 induction한거 cell harvest, sonification, centrifuge 후 soup만 75도에서 1시간 가열, 원심분리 후 soup만 centricon으로 농축 - Taq 정제 25일에 ...")
- 04:10, 17 March 2014 14.2.25 (hist) [77 bytes] Guest (Talk | contribs) (Created page with "24일에 접종한거 1L large scale up 후 induction streaking한거 접종")
- 04:09, 17 March 2014 14.2.24 (hist) [93 bytes] Guest (Talk | contribs) (Created page with "Taq Pol DIY로 만들기 프로젝트 Taq 클로닝 된거 plate에 streaking lb에 접종")
- 04:08, 17 March 2014 14.2.21 (hist) [27 bytes] Guest (Talk | contribs) (Created page with "srtA,mRFP,GFP 다시 정제")
- 04:08, 17 March 2014 14.2.18 (hist) [118 bytes] Guest (Talk | contribs) (Created page with "17일 실험 다시해서 젤 내려봄 assembly 확인 위해 transformation control이 이상해서 망함..")
- 04:06, 17 March 2014 14.2.17 (hist) [102 bytes] Guest (Talk | contribs) (Created page with "Gibson assembly (one-step) 실험 - 직접 만든 master mixure 잘되나 실험 잘 모르겠어..")
- 04:05, 17 March 2014 14.2.14 (hist) [57 bytes] Guest (Talk | contribs) (Created page with "control 용량을 높여서 다시내렸지만 이상함")
- 04:05, 17 March 2014 14.2.12 (hist) [26 bytes] Guest (Talk | contribs) (Created page with "10일꺼 젤 다시 내림")
- 04:04, 17 March 2014 14.2.10 (hist) [121 bytes] Guest (Talk | contribs) (Created page with "Reaction test BSA SrtA GFP RFPwaste Marker B+G+R S+G+R(30m,1h,2h,3h) 젤이 이상해...")
- 04:03, 17 March 2014 14.2.6 (hist) [200 bytes] Guest (Talk | contribs) (Created page with "standard curve 다시 쟤서 단백질 정량 srtA : 0.76-1.05 mg/ml GFP : 20.6 mRFP : 63.48-65.14 mRFP waste : 5.8 몰비율 맞춰서 대충희석해서 다음에 1대1 r...")
- 04:01, 17 March 2014 14.2.5 (hist) [94 bytes] Guest (Talk | contribs) (Created page with "4일에 만든 standard curve로 정량 후 제대로 희석해서 PAGE 내렸으나 이상함")
- 04:00, 17 March 2014 14.2.4 (hist) [113 bytes] Guest (Talk | contribs) (Created page with "1. bradford정량으로 srtA, GFP,mRFP 확인해 보려고 일단 standard curve만듦 2. Double transformation")
- 03:57, 17 March 2014 14.2.3 (hist) [104 bytes] Guest (Talk | contribs) (Created page with "29일ㅇ[ 내린것 많아서 다 끌림 5배 희석 후 로딩 2 5 52 52 52 근데 이것도 끌림..")
- 07:06, 29 January 2014 14.1.29 (hist) [167 bytes] Guest (Talk | contribs) (Created page with "28일에 내린 15% SDS-PAGE gel 결과가 좋지 않아서 다시 내림 단백질이 너무많고, 오래 내려서 그런 것 같아서, 2람다만 내리기로함")
- 07:25, 28 January 2014 14.1.28 (hist) [360 bytes] Guest (Talk | contribs) (Created page with "30과 10의 centricon을 이용한 단백질 농축 및 buffer change 2*Ala-GFP, mRFP1-LPETAA, srtA 농축 및 srt reaction buffer change 후 15% SDS-PAGE gel로 내려봄 ;GFP...")
- 06:59, 28 January 2014 14.1.24 (hist) [81 bytes] Guest (Talk | contribs) (Created page with "23일에 정제한 16도 AIM, SDS-PAGE gel 내리기 -> 성공했다고 합니다")
- 06:58, 28 January 2014 14.1.23 (hist) [47 bytes] Guest (Talk | contribs) (Created page with "21일에 induction한 16도꺼 단백질 정제")
- 06:58, 28 January 2014 14.1.22 (hist) [144 bytes] Guest (Talk | contribs) (Created page with "21일에 induction한 것 단백질정제 전에 bugbuster로 발현확인테스트 후 25도꺼만 단백질 정제 후 SDS-PAGE gel 내리기")
- 06:56, 28 January 2014 14.1.21 (hist) [115 bytes] Guest (Talk | contribs) (Created page with "20일에 접종한 BL21-pJL-srtA, large scale up 및 induction ; 16도 LB 1개, 25도 AIM(Auto-Inducing Media) 1개")
- 06:54, 28 January 2014 14.1.20 (hist) [20 bytes] Guest (Talk | contribs) (Created page with "BL21-pJL-srtA 접종")
- 06:54, 28 January 2014 14.1.17 (hist) [400 bytes] Guest (Talk | contribs) (Created page with "16일에 induction 한 것 3가지를 단백질정제 후 PAGE gel로 내리기 -> crude, loading through, fraction, waste 이 4가지 다 해야하는데 fraction 얻었다고...")
- 06:50, 28 January 2014 14.1.16 (hist) [156 bytes] Guest (Talk | contribs) (Created page with "15일에 접종한 BL21 3가지 large scale up 후 induction ; srtA는 16도에서 나머지는 37도에서 induction 13일에 했던 transformation 다시")
- 06:48, 28 January 2014 14.1.15 (hist) [180 bytes] Guest (Talk | contribs) (Created page with "14일에 접종한 것 중에 BL21 3가지 다시 접종 14일에 접종한 것 중에 DH5a 4가지 에서 plasmid 추출/확인했으나 이상해서 다시 transformation 예정")
- 06:45, 28 January 2014 14.1.13 (hist) [190 bytes] Guest (Talk | contribs) (Created page with "Filamentus bacteria phage 실험 시작 trasformation : BW25113-pATLIC-srtA; BL21(DE3)-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA; DH5a-pATLIC-srtA,-pJL-srtA,-pJL-2*Ala:GFP,-pJL-...")
- 06:48, 13 January 2014 14.1.14 (hist) [182 bytes] Guest (Talk | contribs) (Created page with "sortase 관련 실험!!! trasformation : BW25113-pATLIC-srtA; BL21(DE3)-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA; DH5a-pATLIC-srtA,-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA")
- 06:47, 13 January 2014 1.14 (hist) [175 bytes] Guest (Talk | contribs) (Created page with "sortase 관련 실험!!! trasformation : BW25113-pATLIC-srtA, BL21(DE3)-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA, DH5a-pATLIC-srtA,-pJL-srtA,-pJL-2*Ala:GFP,-pJL-mRFP1:LPETAA")
- 06:21, 29 August 2013 8.28 (hist) [74 bytes] Guest (Talk | contribs) (Created page with "transformation한거 접종 후 plasmid prep 해서 sequencing 보내기")
- 02:47, 27 August 2013 8.27 (hist) [396 bytes] Guest (Talk | contribs) (Created page with "pATLIC vector enzyme cut Nacrein pcr clean up")
- 07:36, 26 August 2013 8.26 (hist) [320 bytes] Guest (Talk | contribs) (Created page with "pATLIC : 단백질 표면발현벡터 File:PATLIC.png 근데 Nacrein은 원래 signal peptide가 달려있는 놈이라 이걸 떼고 클로닝햇어야했느데 안떼고...")
- 07:16, 26 August 2013 8.22 (hist) [159 bytes] Guest (Talk | contribs) (Created page with "cloning 한거가 진짜 plasmid 맞는지 enzyme cut으로 확인 확인용이라 plamsid 4 buffer#4 0.5 EcoR1 0.5 37'c 30min")
- 07:15, 26 August 2013 8.21 (hist) [28 bytes] Guest (Talk | contribs) (Created page with "transformation 한거 접종")
- 07:13, 26 August 2013 8.20 (hist) [956 bytes] Guest (Talk | contribs) (Created page with "pB4가 pJL보다 발현 protein의 solubility를 높여준다. DpN2 : TEMP과 VECTOR의 항생제저항성이 같을 때만 사용한다. 항생제저항성이 다르면 쓸 ...")
- 07:02, 26 August 2013 8.19 (hist) [191 bytes] Guest (Talk | contribs) (Created page with "==pcr== 500mb 짜리 temp 1 -plasmid니깐 10x buffer 1 dNTP 1 primer 1 HQ 5 DW 36 a-Taq ...")
- 05:53, 14 August 2013 8.14 (hist) [634 bytes] Guest (Talk | contribs) (Created page with "pB4 가 transformation이 안됬다 ==Transformation== BL21(DE3) - pB4 DHA-pB4, pRBAOK")
- 07:56, 13 August 2013 8.13 (hist) [297 bytes] Guest (Talk | contribs) (Created page with "Nacrein , Nacrein CA, Nacrein DR, Nacrein R 접종 Transformation DH5a(증폭 및 컨트롤)-pB4, pB2 BL21(DE3)-pB4,pJL(T7 promoter가 있슴) BW2113-pAT(pBAD가 있슴)")
- 03:31, 12 August 2013 LIC 법 (hist) [1,850 bytes] Guest (Talk | contribs) (Created page with "http://bitesizebio.com/articles/get-your-clone-90-of-the-time-with-ligation-independent-cloning/ Ligation Independent Cloning 으로서 conventional cloning 처럼 제한효...")
- 03:08, 12 August 2013 Cloning (hist) [40 bytes] Guest (Talk | contribs) (Created page with "LIC")
- 03:31, 2 August 2013 8.2 (hist) [116 bytes] Guest (Talk | contribs) (Created page with "==TLC solvent만들기== 당 확인용 solvent 3 : 2 : 2 = n-butanol(1-butanol) : ethanol : water (9:6:6, 6:4:4)")
- 03:30, 2 August 2013 TLC (hist) [506 bytes] Guest (Talk | contribs) (Created page with "==TLC(Thin Layer Chromatography)== File:TLC.jpg 1. 이렇게 설정하고, 농도가 적은 sample은 뿌리고 말리고 다시 뿌리고를 반복 7회 정도 2. solve...")
- 02:51, 2 August 2013 8.1 (hist) [435 bytes] Guest (Talk | contribs) (Created page with "TLC(Thin Layer Chromatography)")
- 02:40, 25 July 2013 Electroporation용 (hist) [447 bytes] Guest (Talk | contribs) (Created page with "1. 2x LB에 접종해서 OD 0.6-0.8정도 까지 키우고 얼음에 20분 방치 2. sorvall tube로 옮겨 담고, 3600rpm, 4'C 5min 원심분리 3. cell down된 pellet을 gly...")
- 02:40, 25 July 2013 Gel extraction kit (hist) [398 bytes] Guest (Talk | contribs) (Created page with "원리는 그냥 DNA 추출이랑 비슷 전기영동 후 원하는 부분을 잘라서 젤을 녹인 후 실리카겔에 통과시켜 dna만 남게한 후 washing, elution 해...")
- 02:39, 25 July 2013 7.24 (hist) [910 bytes] Guest (Talk | contribs) (Created page with "==Electroporation comp cell 만들기== 1. 2x LB에 접종해서 OD 0.6-0.8정도 까지 키우고 얼음에 20분 방치 2. sorvall tube로 옮겨 담고, 3600rpm, 4'C 5min 원...")
- 02:52, 24 July 2013 PCR Clean up (hist) [239 bytes] Guest (Talk | contribs) (Created page with "1. 컬럼에 5x volume CB과 pcr한거를 넣고 원심분리 1min 2. NW 750 원심분리 1min + 1min 3. 에탄올 날라가기 기다리기 1min 4. 1.5ml tube로 갈아끼...")
- 02:51, 24 July 2013 7.9 (hist) [256 bytes] Guest (Talk | contribs) (Created page with "==PCR CLEAN UP== 1. 컬럼에 5x volume CB과 pcr한거를 넣고 원심분리 1min 2. NW 750 원심분리 1min + 1min 3. 에탄올 날라가기 기다리기 1min 4. 1.5ml ...")
- 02:46, 24 July 2013 곰팡이 rna 추출 kit (hist) [351 bytes] Guest (Talk | contribs) (Created page with "==곰팡이에서 RNA 추출 kit== 1. 액체질소를 붓고 막자사발로 cell을 grinding 2. RLC 3.lilac 컬럼 원심분리 2min 4. 걸러져 나온 거 + 0.5 volume...")
- 02:45, 24 July 2013 7.23 (hist) [386 bytes] Guest (Talk | contribs) (Created page with "==곰팡이에서 RNA 추출== 1. 액체질소를 붓고 막자사발로 cell을 grinding 2. RLC 3.lilac 컬럼 원심분리 2min 4. 걸러져 나온 거 + 0.5 volume의 1...")
- 06:39, 23 July 2013 RNA 추출 (hist) [111 bytes] Guest (Talk | contribs) (Created page with "http://en.wikipedia.org/wiki/Guanidinium_thiocyanate-phenol-chloroform_extraction")
- 07:41, 22 July 2013 라용언 introduction (hist) [12 bytes] Guest (Talk | contribs) (Created page with "으앙구닌")
- 03:22, 22 July 2013 7.19 (hist) [384 bytes] Guest (Talk | contribs) (Created page with "==PAGE gel만들기== 단백질의 크기가 클수록 낮은 %의 gel을 사용한다. 준비물 : running gel과 stocking gel stocking gel을 좀 더 늦게 만든다-APS랑 ...")
- 02:14, 19 July 2013 Ampicilin (hist) [340 bytes] Guest (Talk | contribs) (Created page with "http://en.wikipedia.org/wiki/Ampicillin 참조 페니실린계 항생제, beta-lactam antibiotic penetrate Gram-positive and some Gram-negative bacteria 세포벽을 합성...")
- 02:11, 19 July 2013 Kanamycin (hist) [332 bytes] Guest (Talk | contribs) (Created page with "http://en.wikipedia.org/wiki/Kanamycin 참조 Kanamycin interacts with the 30S subunit of prokaryotic ribosomes. It induces substantial amounts of mistranslation and indirectly...")
- 09:38, 18 July 2013 Arabinose operon (hist) [379 bytes] Guest (Talk | contribs) (Created page with "아라비도스 대사에 관련있는 구조유전자 BAD를 KO 시킥, 원하는 유전자를 넣은 VECTOR를 만듦 아라비도스를 넣어주어 오페론이 ACTIVATION...")
- 09:30, 18 July 2013 전기영동 (hist) [1,167 bytes] Guest (Talk | contribs) (Created page with "EtBr을 녹인 액체한천을 전기영동 틀애 붓고 comb을 이용해서 14개의 wall을 만든다. 그 위에 젤이 잠기도록 버퍼를 붓는다. ×5 loading dye (lo...")
- 09:22, 18 July 2013 단백질발현실험 (hist) [3,511 bytes] Guest (Talk | contribs) (Created page with "==단백질발현실험== 5. cell harvest : large scale - 500ml bottle 두개에 옮겨 담아서 원심분리 후 냉동보관 cell harvest : small scale - 500 마이크로L...")
- 09:19, 18 July 2013 Sma1 (hist) [74 bytes] Guest (Talk | contribs) (Created page with "Sma1(20U/ul) 1 플라스미드 44 버퍼 5 up to 50")
- 09:19, 18 July 2013 Restriction enzyme (hist) [8 bytes] Guest (Talk | contribs) (Created page with "Sma1")
- 09:18, 18 July 2013 LB (hist) [470 bytes] Guest (Talk | contribs) (Created page with "==LB broth== 100ml : LB 2.5g ==LB agar== 100ml : LB 2.5g, agar 1.5g agar가 용량의 1.5%보다 작으면 묽어져서 spreading 할 때 배지가 백금이에 파일 수가...")
- 09:16, 18 July 2013 MMR (hist) [169 bytes] Guest (Talk | contribs) (Created page with "MMG Agar(100ml) Sdl 배지 2.3 % sea salt 0.1 % yeast extract 0.05 % Ammonium Cloride 50mM Tris-HCl, pH 7.4(최종) up to 100ml DW 1.5g Agar")
- 09:16, 18 July 2013 배지 (hist) [15 bytes] Guest (Talk | contribs) (Created page with "MMR")
- 09:12, 18 July 2013 플라스미드 추출 kit (hist) [692 bytes] Guest (Talk | contribs) (Created page with "=플라스미드 추출= 1. 형질전환 된 cell 3ml를 항생제가 첨가된 LB broth에 현탁 배양")
- 09:11, 18 July 2013 곰팡이 GDNA 추출 (hist) [261 bytes] Guest (Talk | contribs) (Created page with "액화질소처리 후 막자사발로 cell을 물리적으로 먼저 깨고 lysis buffer에 녹인 후 chloroform : Isoamylalchol 유기용매로 수용성(DNA)와 지용성(...")
- 09:10, 18 July 2013 DNA 추출 (hist) [81 bytes] Guest (Talk | contribs) (Created page with "곰팡이 GDNA 추출")
- 09:10, 18 July 2013 Prep kit 원리 (hist) [791 bytes] Guest (Talk | contribs) (Created page with "Slica gel에 DNA가 binding 하는 이유 참고) http://en.wikipedia.org/wiki/DNA_separation_by_silica_adsorption Binding to silica surfaces in the presence of certain salts an...")
- 09:09, 18 July 2013 Alpha-Taq pol (hist) [546 bytes] Guest (Talk | contribs) (Created page with "==Reaction mixture== 10x alpha-Taq reaction buffer 5 uL dNTP mix(2.5mM) 3 uL primer 2 uL templete-genomic DNA 3 u...")
- 09:06, 18 July 2013 PCR (hist) [519 bytes] Guest (Talk | contribs) (Created page with "reaction buffer는 무조건 5 dNTP mix는 1kb당 1 혹은 +1 prime도 2 Templete - copy수가 많은 plasmid DNA의 경우 1 Templete - genomic DNA의 경우 3 alp...")
- 09:03, 18 July 2013 TSS용 (hist) [1,500 bytes] Guest (Talk | contribs) (Created page with "=competent cell 만들기= 준비물 : TSS solution 50ml(85% LB 1.0625g, 10% Polyethylene glycol 5g, 5% DMSO 2.5ml, 50mM MgCl2 anhydrous 0.228g), 2×LB medium 200ml (LB 6.2g, Na...")
- 09:03, 18 July 2013 Competent cell 만들기 (hist) [34 bytes] Guest (Talk | contribs) (Created page with "TSS Electroporation용")
- 09:02, 18 July 2013 TSS (hist) [976 bytes] Guest (Talk | contribs) (Created page with "1. -80°C에서 얼렸던 competent cell을 ice에 넣고 녹인다. ice cold competent cell 50마이크로L와 8.8ng/마이크로L짜리 PUC19 플라스미드 DNA 1마이크로L...")
- 09:01, 18 July 2013 Electroporation (hist) [821 bytes] Guest (Talk | contribs) (Created page with "전기충격으로 membrane을 흐물흐물하게 만든 후 DNA를 transformation하는 방법으로, cell이 죽기 쉬우나 efficiency가 높다. 과전류가 흘러서 cell...")
- 09:01, 18 July 2013 Transformation (hist) [28 bytes] Guest (Talk | contribs) (Created page with "TSS Electroporation")
- 08:58, 18 July 2013 7.18 (hist) [841 bytes] Guest (Talk | contribs) (Created page with "==Electroporation== 전기충격으로 membrane을 흐물흐물하게 만든 후 DNA를 transformation하는 방법으로, cell이 죽기 쉬우나 efficiency가 높다. 과전...")
- 08:13, 18 July 2013 PET Expression System (hist) [458 bytes] Guest (Talk | contribs) (Created page with "http://www.bio.davidson.edu/Courses/Molbio/MolStudents/spring2003/Causey/pET.html")
- 08:06, 18 July 2013 항생제 (hist) [28 bytes] Guest (Talk | contribs) (Created page with "ampicilin kanamycin")
- 08:05, 18 July 2013 과발현법 (hist) [47 bytes] Guest (Talk | contribs) (Created page with "Arabinose operon pET Expression System")
- 08:00, 18 July 2013 공부 (hist) [80 bytes] Guest (Talk | contribs) (Created page with "항생제 과발현법")
- 05:52, 17 July 2013 7.17 (hist) [571 bytes] Guest (Talk | contribs) (Created page with "==PCR== reaction buffer는 무조건 5 dNTP mix는 1kb당 1 혹은 +1 prime도 2 Templete - copy수가 많은 plasmid DNA의 경우 1 - genomic DNA의 경우 3...")
- 05:44, 17 July 2013 7.16 (hist) [145 bytes] Guest (Talk | contribs) (Created page with "==항생제 stock 만들기== 항생제 물에 녹이고 멸균필터처리 autoclave 돌리면 항생제 으앙쥬금되니깐 멸균필터로")
- 05:33, 15 July 2013 7.15 (hist) [645 bytes] Guest (Talk | contribs) (Created page with "==colony PCR== 1. 20ul DW에 colony를 적당량 섞고 2. 95'C에서 5분 3. centrifuge maxi 3분 4. soup을 templete로 4ul 5. alpha-Taq PCR")
- 04:57, 8 July 2013 7.8 (hist) [857 bytes] Guest (Talk | contribs) (0)
- 06:06, 25 April 2013 CRISPR (hist) [417 bytes] Igchoi (Talk | contribs) (Created page with "==Readings== [http://www.sciencemag.org/content/339/6121/768.summary Science] [http://elife.elifesciences.org/content/2/e00563.abstract eLife]")